GROWTH  PERFORMANCE OF TRANSFECTION TIGER SHRIMP  BROODSTOCK Penaeus monodon  IN DIFFERENT GENERATION

Diseases resistant genes assemblage for tiger shrimp has been initiated by The Research Institute for Coastal Aquaculture in collaboration with Bogor Agricultural Institute, through transgenesis approach under anti-virus genes transfection. The study aimed to evaluate the growth performance of broodstock candidates of tiger shrimp at different generati on (F0 and F1). This research was conducted at 2000 m2size of four ponds in Takalar Regency, South Sulawesi.. The treatment was different generations of broodstocks, which were: broodstocks originated from F0generation, (A) and F1 (B). The 22.63 to 28.57 g of broodstock candidates were stocked 0.5 ind.m-2 and then reared for 128 days. During rearing period, these shrimp were fed usingcommercial pelleted feed with content 36-38% of protein in dosage of 10-4%/ body weight. Feeding frequency was applied in the morning and in the evening. Measured variables were growth, size distribution, survival rate and water quality The results indicated that the performances of these shrimps, growth, size distributions as well as survival rates between these F0 and F1 were not significantly different (p>0.05). The growth pattern was relatively equal between treatment during rearing period. Survival rate of tiger shrimp in this study ranged from 51.7 to 73.35%. This study have implications on the provision of superior broodstock shrimp in ponds in order to support the sustainability of shrimp seed production in hatchery. Keywords: broodstock, production, transgenic, tiger shrimp

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GROWTH PERFORMANCE OF TRANSFECTION TIGER SHRIMP BROODSTOCK Penaeus monodon IN DIFFERENT GENERATION

Jurnal Ilmu dan Teknologi Kelautan Tropis ◽

10.29244/jitkt.v9i1.17932 ◽

2017 ◽

Vol 9 (1) ◽

pp. 185-199 ◽

Cited By ~ 1

Author(s):

Hidayat Suryanto Suwoyo ◽

. Sahabuddin

Keyword(s):

Survival Rate ◽

Growth Performance ◽

Survival Rates ◽

Penaeus Monodon ◽

Size Distributions ◽

Feeding Frequency ◽

South Sulawesi ◽

Tiger Shrimp ◽

Coastal Aquaculture ◽

Pelleted Feed

Diseases resistant genes assemblage for tiger shrimp has been initiated by The Research Institute for Coastal Aquaculture in collaboration with Bogor Agricultural Institute, through transgenesis approach under anti-virus genes transfection. The study aimed to evaluate the growth performance of broodstock candidates of tiger shrimp at different generati on (F0 and F1). This research was conducted at 2000 m2size of four ponds in Takalar Regency, South Sulawesi.. The treatment was different generations of broodstocks, which were: broodstocks originated from F0generation, (A) and F1 (B). The 22.63 to 28.57 g of broodstock candidates were stocked 0.5 ind.m-2 and then reared for 128 days. During rearing period, these shrimp were fed usingcommercial pelleted feed with content 36-38% of protein in dosage of 10-4%/ body weight. Feeding frequency was applied in the morning and in the evening. Measured variables were growth, size distribution, survival rate and water quality The results indicated that the performances of these shrimps, growth, size distributions as well as survival rates between these F0 and F1 were not significantly different (p>0.05). The growth pattern was relatively equal between treatment during rearing period. Survival rate of tiger shrimp in this study ranged from 51.7 to 73.35%. This study have implications on the provision of superior broodstock shrimp in ponds in order to support the sustainability of shrimp seed production in hatchery. Keywords: broodstock, production, transgenic, tiger shrimp

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Growth of tiger shrimp Penaeus monodon post-larvae fed on Artemia containing Vibrio SKT-b probiotic

Jurnal Akuakultur Indonesia ◽

10.19027/jai.12.79-85 ◽

2014 ◽

Vol 12 (1) ◽

pp. 79 ◽

Cited By ~ 1

Author(s):

. Widanarni ◽

Yani Hadiroseyani ◽

Asri Sutanti

Keyword(s):

Growth Rate ◽

Body Weight ◽

Survival Rate ◽

Sea Water ◽

Penaeus Monodon ◽

Environmentally Friendly ◽

Probiotic Bacteria ◽

Growth And Survival ◽

Tiger Shrimp ◽

Shrimp Production

ABSTRACT Application of probiotic bacteria is an alternative technology to increase shrimp production in an environmentally friendly aquaculture. Administration of probiotic bacteria can be conducted through artificial feed or live food such as Artemia. This study was done to examine the effectiveness of various doses of probiotic Vibrio SKT-b through Artemia on the growth and survival of post-larval shrimp. Tiger shrimp at a stage of PL 10 was reared in glass jars filled with 2 L of sea water at a density of 10 larva/L. The study consisted of five probiotic concentrations control (0 cfu/mL), A (103 cfu/mL), B (104 cfu/mL), C (105 cfu/mL), and D (106 cfu/mL). Administration of various doses of probiotic bacteria Vibrio SKT-b through Artemia significantly increased the growth rate in term of the length and weight, but had no effect on survival. The results found that treatment D (106 cfu/mL) gave an increase in body weight, length and survival rate of 22.53%/day, 0.080 cm/day and 95%, respectively. Keywords: probiotic, Artemia, tiger shrimp ABSTRAK Aplikasi bakteri probiotik merupakan salah satu alternatif teknologi untuk meningkatkan produksi budidaya udang yang ramah lingkungan. Pemberian bakteri probiotik dapat dilakukan melalui pakan buatan atau pakan alami seperti Artemia. Penelitian ini bertujuan untuk menguji efektivitas pemberian berbagai dosis bakteri probiotik Vibrio SKT-b melalui Artemia terhadap pertumbuhan dan kelangsungan hidup pascalarva udang windu. Stadia udang windu yang digunakan adalah pascalarva (PL) 10. Udang dipelihara dalam wadah kaca volume 3 L yang diisi air laut 2 L dengan kepadatan 10 ekor/L. Penelitian ini terdiri atas lima perlakuan yaitu kontrol (dosis bakteri probiotik 0 cfu/mL), A (103 cfu/mL), B (104 cfu/mL), C (105 cfu/mL), dan D (106 cfu/mL). Hasil penelitian menunjukkan bahwa pemberian berbagai dosis bakteri probiotik Vibrio SKT-b melalui Artemia berpengaruh nyata terhadap laju pertumbuhan panjang dan bobot, namun tidak berpengaruh terhadap nilai kelangsungan hidup. Hasil terbaik diperoleh pada perlakuan D (106 cfu/mL) dengan pertumbuhan bobot 22,53%/hari, pertumbuhan panjang 0,08 cm/hari, dan sintasan 95%. Kata kunci: probiotik, Artemia, udang windu

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Growth and survival of transfection and non-transfection tiger shrimp (Penaeus monodon Fabricius) broodstock candidate in pond cultivation

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/890/1/012038 ◽

2021 ◽

Vol 890 (1) ◽

pp. 012038

Author(s):

Suwardi ◽

H S Suwoyo

Keyword(s):

Survival Rate ◽

Size Distribution ◽

Penaeus Monodon ◽

Stocking Density ◽

Growth And Survival ◽

South Sulawesi ◽

Tiger Shrimp ◽

Commercial Species ◽

Pelleted Feed

Abstract Tiger shrimp (Penaeus monodon Fab.) is one of important commercial species in Indonesia. Low quality of shrimp fry due to the lack of good quality broodstock availability is shrimp cultivation problem that need to be solved. The study was aimed to evaluate growth and survival rate of broodstock candidate of transfection and non-transfection tiger shrimp. The study was conducted using four ponds with 2000 m2 in size in Takalar Regency. South Sulawesi. The treatment were A = transfection tiger shrimp broodstock candidate and B = non-transfection tiger shrimp broodstock candidate. Approximately 19.0-21.9 g/ind of shrimp were reared in 60 days in 0.25 individu/m2 (500 individu/2000 m2) stocking density. Commercial pelleted feed which content 38% of protein was fed three times a day in 5-3% of body weight dosage. Measured variables were growth performance, size distribution and survival rate. The result showed that shrimp growth were no significantly different (P>0.05), while survival rate and size distribution were significantly different (P<0.05). The dominant size of transfection shrimp was 41-50 g/ind. Whereas, non-transfection shrimp was dominated by 31-40 g/ind in size (48.39%). Survival rate of tiger shrimp broodstock candidate in this study ranged from 30.80 to 64.10%.

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PERFORMA BUDIDAYA UDANG WINDU (Penaeus monodon) PADA MUSIM KEMARAU DI TAMBAK KECAMATAN MARUSU KABUPATEN MAROS

Media Akuakultur ◽

10.15578/ma.16.1.2021.45-56 ◽

2021 ◽

Vol 16 (1) ◽

pp. 45

Author(s):

Andi Akhmad Mustafa ◽

Andi Indra Jaya Asaad ◽

Dominggus Linthin

Keyword(s):

Survival Rate ◽

Penaeus Monodon ◽

Dry Season ◽

Shrimp Farming ◽

Average Weight ◽

Feed Conversion ◽

Shrimp Culture ◽

South Sulawesi ◽

Tiger Shrimp ◽

Aquaculture Development

Salah satu program dari Gubernur Sulawesi Selatan periode 2018-2023 adalah mengembalikan kejayaan udang windu di Sulawesi Selatan. Telah dilakukan kegiatan yang bertujuan mengetahui performa budidaya udang windu di tambak pembudidaya yang dilaksanakan pada musim kemarau tahun 2019 di Kecamatan Marusu Kabupaten Maros. Kegiatan budidaya udang windu teknologi tradisional plus dilaksanakan di tambak Dusun Kurilompo, Desa Nisombalia Kecamatan Marusu Kabupaten Maros. Sebanyak enam petak tambak pembudidaya ditebari tokolan udang windu dengan padat penebaran dari 10.000 sampai 30.000 ekor/ha. Setelah dipelihara selama 69 sampai 80 hari dilakukan panen dan selanjutnya dilakukan penentuan sintasan, bobot rata-rata, produksi udang windu, dan rasio konversi pakan (RKP). Data yang diperoleh dianalisis secara deskriptif. Hasil kegiatan menunjukkan bahwa kualitas air di tambak Kecamatan Marusu, Kabupaten Maros pada musim kemarau dicirikan dengan salinitas yang berkisar antara 36,63-69,40 ppt; suatu kondisi yang tidak optimal untuk budidaya udang windu. Pada saat panen didapatkan sintasan udang windu dari 27,57% sampai 66,67%; bobot rata-rata dari 14,29-40,00 g/ekor; produksi dari 90,1-800,0 kg/ha/musim; dan RKP dari 0,54:1 sampai 2,10:1. Disimpulkan bahwa budidaya udang windu di tambak bersalinitas tinggi di daerah ini merupakan penyebab utama rendahnya sintasan, bobot rata-rata, produksi udang windu, dan RKP. Direkomendasikan bahwa perbaikan budidaya udang dengan menjaga tingkat salinitas dalam nilai optimal harus disosialisasikan kepada pembudidaya udang windu di daerah tersebut.One of the main aquaculture development targeted by the South Sulawesi Province for the period 2018-2023 is to restore the glory of tiger shrimp farming in South Sulawesi. The recent study was carried out to uncover redevelopment opportunities of tiger shrimp culture and determine the performance of tiger shrimp culture in the farmers’ ponds. The study was performed during the dry season in Marusu Subdistrict Maros District, in 2019. Traditional plus tiger shrimp farming activities were carried out in the ponds of Kurilompo Subvillage, Nisombalia Village, Marusu Subdistrict, Maros District. A total of six brackishwater ponds were stocked with tiger shrimp fingerling with stocking densities of 10,000-30,000 ind./ha. After being cultured for 69 to 80 days, the shrimp were harvested and measured their survival rate, average weight, production of tiger shrimp, and feed conversion ratio (FCR). The data obtained were analyzed with descriptive statistics. The results showed that water quality in brackishwater ponds of Marusu Subdstrict Maros District, in the dry season is characterized by salinity ranging from 36.63 to 69.40 ppt, a condition that is not optimal for tiger shrimp culture. At harvest, the survival rate, average weight, production, and FCR of the farmed average tiger shrimp were 27.57%-66.67%, 14.29-40.00 g/ind., 90.1-800.0 kg/ha/season, and 0.54:1-2.10:1, respectively. It is concluded that the culture of tiger shrimp in the brackishwater ponds with high salinity in this area is the primary cause of poor survival rate, average weight, production of tiger shrimp, and FCR. It is recommended that culture shrimp improvement via maintaining salinity level within the optimal values has to be disseminated to the fish farmers in the area.

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Improved pond productivity through integrated cultivation of red tilapia (Oreochromis niloticus), tiger shrimp (Penaeus monodon) and seaweed (Gracilaria verrucosa) in Maros, South Sulawesi, Indonesia

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/521/1/012001 ◽

2020 ◽

Vol 521 ◽

pp. 012001

Author(s):

B Pantjara ◽

A Sarijanah ◽

M Hermawan

Keyword(s):

Oreochromis Niloticus ◽

Penaeus Monodon ◽

Gracilaria Verrucosa ◽

Red Tilapia ◽

South Sulawesi ◽

Tiger Shrimp

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RESPONS IMUN UDANG WINDU Penaeus monodon TERHADAP VAKSIN dsRNA VP-24 PADA DOSIS BERBEDA

Jurnal Riset Akuakultur ◽

10.15578/jra.13.1.2018.77-84 ◽

2018 ◽

Vol 13 (1) ◽

pp. 77

Author(s):

Sri Redjeki Hesti Mulyaningrum ◽

Andi Parenrengi ◽

Bunga Rante Tampangallo ◽

Ike Trismawanti

Keyword(s):

Immune Response ◽

Survival Rate ◽

Saline Solution ◽

Penaeus Monodon ◽

Challenge Test ◽

Injection Method ◽

Tiger Shrimp ◽

Total Hemocyte Count ◽

Hemocyte Count

Peningkatan produksi udang windu Penaeus monodon terus diupayakan, salah satunya dengan peningkatan respons imun udang terhadap infeksi penyakit WSSV. Penelitian ini bertujuan untuk mengetahui respons imun udang terhadap pemberian vaksin dsRNA VP-24 pada berbagai dosis. Konstruksi vaksin dsRNA VP-24 dilakukan menggunakan Megascript kit dengan DNA genom VP-24 sebagai template. Vaksinasi dilakukan dengan metode injeksi pada udang windu yang berukuran rata-rata 15,88 ± 3,50 g; dosis vaksin yang diujikan adalah 0,02 µg; 0,2 µg; 2 µg; dan sebagai kontrol adalah udang yang tidak diberi vaksin. Penelitian terdiri atas empat perlakuan dosis vaksin dengan masing-masing dua ulangan dan dipelihara selama lima hari. Uji tantang dilakukan selama enam hari dengan menginjeksi virus WSSV dalam saline solution (1:3 v/v). Pengamatan terhadap sintasan udang windu dilakukan setiap hari, sedangkan penghitungan total hemocyte (THC) dan ProPO diamati pada hari I, III, dan VI setelah diinfeksi WSSV. Pada akhir pengujian dilakukan pengambilan jaringan hepatopankreas untuk analisis histopatologi. Analisis data dilakukan secara statistik dengan analisis ragam (ANOVA). Hasil yang diperoleh memperlihatkan bahwa injeksi vaksin dsRNA VP-24 dengan dosis 0,2 µg memiliki pengaruh yang signifikan terhadap sintasan dan respons imun udang windu (P<0,05). Vaksin dsRNA VP-24 dengan dosis 0,2 µg mampu memberikan sintasan udang windu P. monodon sebesar 65% dan meningkatkan respons imun udang dengan THC (1.550 x 10t sel/mL) dan ProPO (0,042 Abs).One of the efforts to increase the production of tiger shrimp Penaeus monodon is increasing the immune response against WSSV disease. This study aims to evaluate shrimp immune response to dsRNA VP-24 vaccination at various doses. The construction of dsRNA VP-24 vaccine was performed using Megascript kit with the VP-24 DNA genome as a template. The vaccination was done by injection method on shrimp sized 15.88 ± 3.50 g. The tested vaccine doses (treatments) were 0.02 ¼g; 0.2 ¼g; 2 ¼g; and unvaccinated shrimp as the control. The study consisted of four treatments of vaccine doses with two replicates for each treatment. The challenge test was performed by injecting the WSSV virus in saline solution (1:3 v/v). The observation on shrimp survival rate was done daily, while the total hemocyte count (THC) and ProPO observation were performed on the 1st day, 3rd day, and 6th day after WSSV infection. At the end of the experiment, samplings of hepatopancreas for analysis were performed. Data were statistically analyzed using ANOVA. The present study indicated that the injection of 0.2 ¼ g dsRNA VP-24 vaccine had a significant effect on the survival rate and immune response of shrimp (P<0.05). The dose of 0.2 ¼g dsRNA VP-24 had resulted in 65% of survival rate and increased immune response of P. monodon with THC (1,550 x 10t cell/mL) and ProPO (0.042 Abs).

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APLIKASI INSEMINASI BUATAN PADA UDANG WINDU, Penaeus monodon ALAM MENGGUNAKAN SUMBER DAN JUMLAH SPERMATOFOR YANG BERBEDA

Jurnal Riset Akuakultur ◽

10.15578/jra.11.3.2016.271-280 ◽

2017 ◽

Vol 11 (3) ◽

pp. 271

Author(s):

Samuel Lante ◽

Asda Laining

Keyword(s):

Reproductive Performance ◽

Artificial Insemination ◽

Similar Condition ◽

Penaeus Monodon ◽

Hatching Rate ◽

Black Tiger Shrimp ◽

Wild Male ◽

South Sulawesi ◽

Tiger Shrimp ◽

Different Sources

Salah satu kendala utama dalam domestikasi udang windu adalah rendahnya tingkat perkawinan secara alami dalam wadah budidaya. Hal yang sama terjadi pada udang windu alam yang digunakan di unit pembenihan. Salah satu upaya untuk mendapatkan telur fertil adalah melalui inseminasi buatan (IB). Inseminasi buatan merupakan teknik mentransfer spermatofor dari induk jantan dengan cara memasukkannya ke dalam telikum udang betina. Penelitian ini bertujuan untuk mengetahui performa reproduksi udang windu betina alam pasca-inseminasi menggunakan sumber dan jumlah spermatofor induk jantan alam yang berbeda. Penelitian dilakukan dua tahap yaitu 1) IB menggunakan spermatofor induk jantan dari perairan Sulawesi Selatan (SS) dan spermatofor induk jantan dari Aceh (SA) dan 2) IB menggunakan jumlah spermatofor berbeda yaitu satu spermatofor (S-1) dan dua spermatofor (S-2) pada udang windu betina alam. Inseminasi spermatofor dilakukan pada induk udang windu betina setelah dua hari moulting. Hasil yang diperoleh pada IB tahap pertama menunjukkan bahwa daya tetas telur udang windu betina alam lokal tidak dipengaruhi oleh sumber (lokasi) asal udang jantan, di mana daya tetas telur relatif sama pada kedua perlakuan, yaitu 61,6% pada SS dan 61,7% pada SA. IB pada tahap kedua menunjukkan bahwa daya tetas telur fertil yang diperoleh pada S-2 sebesar 40,5%; lebih rendah dari S-1 sebesar 44%.One of the main constraints in the domestication of black tiger shrimp is very low natural mating in the tank. Similar condition have been happened in commercial hatcheries. An effort to improve the eggs fertility is through artificial insemination (AI). This study aimed to know reproductive performance of wild black tiger shrimp after insemination with different sources and numbers of spermatophore. This study consisted of two trials.The first one was AI using spermatophores of wild male obtained from two different locations, namely from South Sulawesi (SS) and Aceh (SA). The second trial was AI using different numbers of spermatophore namely one spermatophore (S-1) and two spermatophores (S-2). AI was applied to the females at two days post-moulting. The results of the first trial showed that the hatching rate (HR) was not affected by the source of the male which was 61.6% for SS and 61.7% for SA. The second trial indicated that female inseminated S-2 had lower HR than S-1 (40.5% vs 44%).

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Response of hemocytes profile in the black tiger shrimp (Penaeus monodon) against Vibrio harveyi induced by Xylocarpus granatum leaves extract

Veterinary World ◽

10.14202/vetworld.2020.751-757 ◽

2020 ◽

Vol 13 (4) ◽

pp. 751-757 ◽

Cited By ~ 3

Author(s):

Gina Saptiani ◽

A. Syafei Sidik ◽

Fikri Ardhani ◽

Esti Handayani Hardi

Keyword(s):

Survival Rate ◽

Vibrio Harveyi ◽

Penaeus Monodon ◽

Water Extract ◽

Negative Control ◽

Distilled Water ◽

Black Tiger Shrimp ◽

Xylocarpus Granatum ◽

Tiger Shrimp ◽

Immersing Method

Aim: The present study investigated hemocytes profile of black tiger shrimp (Penaeus monodon) induced with Xylocarpus granatum leaves extract to protect against Vibrio harveyi infection. Materials and Methods: X. granatum leaves were chopped into small size, air-dried, and extracted with one of the following solvents: Ethanol, distilled water, and seawater, whereas each solvent was given in three different concentrations (750 ppm, 1.000 ppm, and 1.250 ppm, respectively). Extracts were induced to 60 post-larvae shrimp in each treatment (three replicates, 20 shrimp for each) by immersing method and subsequently challenged with V. harveyi. Results: This study demonstrated different effectiveness among solvents used to extract X. granatum leaves, in which distilled water showed the most effective solvent as can be seen from the lowest percentage on anorexia, lethargic, and weakened reflex of shrimp compared with another solvent, positive and negative controls. Pathological symptoms for shrimp induced by X. granatum leaves extract were minimum with the highest survival rate compared with those of positive and negative control. Total hemocyte cells and its cell constituents such as semi-granular, granular, and hyaline cells on treatment group at 1.250 ppm were higher than controls. Conclusion: Leaves extract of X. granatum extract effectively inhibited V. harveyi infection, increased survival rate, and hemocytes cell of the experimental shrimp. Distilled water extract of X. granatum at 1.250 ppm demonstrated the highest protective effect toward V. harveyi infection on P. monodon.

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Peningkatan Respon Imun Non-Spesifik Benih Kerapu Bebek, Cromileptes altivelis dengan Imunostimulan dan Bakterin terhadap Infeksi Viral Nervous Necrosis (VNN)

Jurnal Perikanan Universitas Gadjah Mada ◽

10.22146/jfs.160 ◽

2006 ◽

Vol 8 (1) ◽

pp. 25

Author(s):

Des Roza ◽

Fris Johnny ◽

Tridjoko Tridjoko

Keyword(s):

Immune Response ◽

Body Weight ◽

Survival Rate ◽

Intramuscular Injection ◽

Survival Rates ◽

Control Parameters ◽

Treatment Results ◽

Viral Nervous Necrosis ◽

Randomized Design ◽

Completely Randomized Design

An experiment to evaluate the effectiveness immunostimulant and bacterin on humpback grouper have been conducted at the Disease Laboratory of Research Institute for Mariculture, Gondol, Bali. The experiment was designed in completely randomized design with four treatments in duplicates. Two hundred of humpback grouper juveniles (15-18 cm of total length, 55-65 g of body weight) were injected intraperitoneally with (A) bacterine at 107 cfu/kg body weight (BW), (B) peptidoglycan at 100 mg/kg BW, and (C) immuno star at 100 ml/kg BW, (D) control. The fish were then challenged with VNN by intramuscular injection at 10 days post treatment. Results showed that survival rates of juvenile after challenged with VNN were 60.00% (B & C), 53.34% (A), and 1.67% (control). Parameters of non-spesific immune respons showed that phagocytic activities were 17.56% (B), 17.55% (C), 13.11% (A), and 9.33% (control). In addition, lisozyme activities were 1.64 cm (B), 1.58 cm (C), 1.55 cm (A), and 1.46 cm (control). Immunostimulant and bacterin stimulated non-specific immune response, and increased survival rate of humpback grouper juvenile.

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Genetic Evaluation of Body Weight and Survival of Black Tiger Shrimp (Penaeus monodon) fed on Different Dietary Levels of Fish Meal Protein

Pakistan Journal of Zoology ◽

10.17582/journal.pjz/20200423140440 ◽

2020 ◽

Author(s):

Song Jiang

Keyword(s):

Body Weight ◽

Fish Meal ◽

Penaeus Monodon ◽

Genetic Evaluation ◽

Black Tiger Shrimp ◽

Tiger Shrimp ◽

Meal Protein

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