scholarly journals Growth and survival of transfection and non-transfection tiger shrimp (Penaeus monodon Fabricius) broodstock candidate in pond cultivation

2021 ◽  
Vol 890 (1) ◽  
pp. 012038
Author(s):  
Suwardi ◽  
H S Suwoyo

Abstract Tiger shrimp (Penaeus monodon Fab.) is one of important commercial species in Indonesia. Low quality of shrimp fry due to the lack of good quality broodstock availability is shrimp cultivation problem that need to be solved. The study was aimed to evaluate growth and survival rate of broodstock candidate of transfection and non-transfection tiger shrimp. The study was conducted using four ponds with 2000 m2 in size in Takalar Regency. South Sulawesi. The treatment were A = transfection tiger shrimp broodstock candidate and B = non-transfection tiger shrimp broodstock candidate. Approximately 19.0-21.9 g/ind of shrimp were reared in 60 days in 0.25 individu/m2 (500 individu/2000 m2) stocking density. Commercial pelleted feed which content 38% of protein was fed three times a day in 5-3% of body weight dosage. Measured variables were growth performance, size distribution and survival rate. The result showed that shrimp growth were no significantly different (P>0.05), while survival rate and size distribution were significantly different (P<0.05). The dominant size of transfection shrimp was 41-50 g/ind. Whereas, non-transfection shrimp was dominated by 31-40 g/ind in size (48.39%). Survival rate of tiger shrimp broodstock candidate in this study ranged from 30.80 to 64.10%.

2017 ◽  
Vol 9 (1) ◽  
pp. 185-199 ◽  
Author(s):  
Hidayat Suryanto Suwoyo ◽  
. Sahabuddin

Diseases resistant genes assemblage for tiger shrimp has been initiated by The Research Institute for Coastal Aquaculture in collaboration with Bogor Agricultural Institute, through transgenesis approach under anti-virus genes transfection. The  study aimed to evaluate the growth performance of broodstock candidates of tiger shrimp at different generati on (F0 and F1). This research was  conducted at 2000 m2size of four ponds in Takalar Regency, South Sulawesi..  The treatment was  different generations of broodstocks, which were: broodstocks originated from F0generation, (A) and F1 (B). The 22.63 to 28.57 g of broodstock candidates were stocked 0.5 ind.m-2 and then reared for 128 days. During rearing period, these shrimp were fed usingcommercial pelleted feed with content 36-38% of protein in dosage of 10-4%/  body weight. Feeding frequency was applied in the morning and in the evening. Measured variables were growth, size distribution, survival rate  and water quality The results indicated that the performances of these shrimps, growth, size distributions as well as survival rates between these F0 and F1 were not significantly different (p>0.05). The growth pattern was relatively equal between treatment  during rearing period. Survival rate of tiger  shrimp in this study ranged from 51.7 to 73.35%. This study have implications on the provision of superior broodstock shrimp in ponds in order to support the sustainability of shrimp seed production in hatchery. Keywords: broodstock, production, transgenic,  tiger shrimp


Author(s):  
Ali Usman ◽  
Rochmady Rochmady

One of the factors that affect the growth of tiger shrimp is the quality of feed. Nutrient content in feed such as protein, carbohydrate and fat, generally used as a parameter of feed quality. Feed consumed by shrimp is not all digested, one of which depends on the enzymatic ability of the shrimp digestive tract. The use of probiotic bacteria in addition to having the ability to reduce colonies, inhibit the growth of pathogenic bacteria, and neutralize water quality, probiotic bacteria can help digestibility of the intestine. The aim of this research is to know the growth and survival of post larvae of shrimp (Penaeus monodon Fabr.) Through the provision of probiotics with different doses. The research was conducted at Balai Fish Fishing Hall of Muna Regency, Southeast Sulawesi, Indonesia. Post larva shrimp tiger (PL-20) of 120 tails, maintained for 28 days. The tested treatment was probiotic dose 5 ml (treatment A), dose 10 ml treatment B), dose 15 ml (treatment C), and without probiotics (control). Probiotics are given every 7 days on water maintenance media using a pipette (0.5-20 ml scale). Weighing results showed relatively high post-larva shrimp larvae growth of 400.00% (treatment B); 291.67% (treatment C); of 233.33% (treatment A) and equal to 58.33% (Control). Variation analysis result showed that giving of probiotic with different dose gave real effect (P<0,01) to relative growth of postlarva prawn weights (P. monodon Fabr.). Provision of probiotics has no significant effect on postlarva tiger prawn survival rate (P. monodon Fabr.). The survival rate of post larvae of tiger shrimp during the study was in the range 66.67-83.33%.


2018 ◽  
Vol 6 (2) ◽  
pp. 699
Author(s):  
Muhammad Ridho Taris ◽  
Limin Santoso ◽  
Esti Harpeni

Availability of good-quality shrimp feed is an important factor of shrimp increased production. High protein feed can be generated by adding raw materials that contains high protein. One of the raw materials that contains high protein is earthworm meal (Lumbricus sp.). This research goal was to study the influence of the substitution of fish meal by earthworm feed with different proportions toward growth and survival rate of tiger shrimp larvae (Penaeus monodon). The experimental design used Completely Randomized Design (RAL) consists of five treatments with three replications: Feed test A (25%  earthworm meal + 75% fish meal), feed test B (50% earthworm meal + 50% fish meal), feed test C (75% earthworms meal + 25% fish meal), feed test D (100% earthworms meal) and feed test E (commercial feed). Results showed that the tiger shrimp which given feed test E is the best result for tiger shrimp growth with 0.480 ± 0.076 grams/individual, while feed test B was the best result for tiger shrimp survival rate with 82 ± 3.46%., and on the feed test D was the best result for tiger shrimp feed conversion rate with 4.0 ± 0.17. Based on results of statistical tests showed that the substitution of fish meal with earthworm meal did not give significant effect on growth and survival rate of tiger shrimp.


2014 ◽  
Vol 12 (1) ◽  
pp. 79 ◽  
Author(s):  
. Widanarni ◽  
Yani Hadiroseyani ◽  
Asri Sutanti

<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p class="NoParagraphStyle">Application of probiotic bacteria is an alternative technology to increase shrimp production in an environmentally friendly aquaculture. Administration of probiotic bacteria can be conducted through artificial feed or live food such as <em>Artemia</em>. This study was done to examine the effectiveness of various doses of probiotic <em>Vibrio</em> SKT-b through <em>Artemia</em> on the growth and survival of post-larval shrimp. Tiger shrimp at a stage of PL 10 was reared in glass jars filled with 2 L of sea water at a density of 10 larva/L. The study consisted of five probiotic concentrations control (0 cfu/mL), A (10<sup>3</sup> cfu/mL), B (10<sup>4</sup> cfu/mL), C (10<sup>5</sup> cfu/mL), and D (10<sup>6</sup> cfu/mL). Administration of various doses of probiotic bacteria <em>Vibrio</em> SKT-b through <em>Artemia</em> significantly increased the growth rate in term of the length and weight, but had no effect on survival. The results found that treatment D (10<sup>6</sup> cfu/mL) gave an increase in body weight, length and survival rate of 22.53%/day, 0.080 cm/day and 95%, respectively.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: probiotic, <em>Artemia</em>, tiger shrimp</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Aplikasi bakteri probiotik merupakan salah satu alternatif teknologi untuk meningkatkan produksi budidaya udang yang ramah lingkungan. Pemberian bakteri probiotik dapat dilakukan melalui pakan buatan atau pakan alami seperti <em>Artemia</em>. Penelitian ini bertujuan untuk menguji efektivitas pemberian berbagai dosis bakteri probiotik <em>Vibrio</em> SKT-b melalui <em>Artemia</em> terhadap pertumbuhan dan kelangsungan hidup pascalarva udang windu. Stadia udang windu yang digunakan adalah pascalarva (PL) 10. Udang dipelihara dalam wadah kaca volume 3 L yang diisi air laut 2 L dengan kepadatan 10 ekor/L. Penelitian ini terdiri atas lima perlakuan yaitu kontrol (dosis bakteri probiotik 0 cfu/mL), A (10<sup>3</sup> cfu/mL), B (10<sup>4</sup> cfu/mL), C (10<sup>5</sup> cfu/mL), dan D (10<sup>6</sup> cfu/mL). Hasil penelitian menunjukkan bahwa pemberian berbagai dosis bakteri probiotik <em>Vibrio</em> SKT-b melalui <em>Artemia</em> berpengaruh nyata terhadap laju pertumbuhan panjang dan bobot, namun tidak berpengaruh terhadap nilai kelangsungan hidup. Hasil terbaik diperoleh pada perlakuan D (10<sup>6</sup> cfu/mL) dengan pertumbuhan bobot 22,53%/hari, pertumbuhan panjang 0,08 cm/hari, dan sintasan 95%.</p><p class="NoParagraphStyle"> </p><p>Kata kunci: probiotik, <em>Artemia</em>, udang windu</p>


2016 ◽  
Vol 1 (1) ◽  
pp. 55
Author(s):  
Syarifuddin Tonnek ◽  
Markus Mangampa ◽  
Muslimin Muslimin

Produksi tokolan udang windu PL 54 menggunakan hapa pada unit keramba jaring apung (KJA) di laut telah dilakukan di Teluk Labuange, Kabupaten Barru dari bulan Mei—Juli 2002. Penelitian ini bertujuan mendapatkan padat penebaran terbaik untuk pendederan benur udang windu dalam KJA di laut. Perlakuan yang dicobakan adalah padat penebaran benur PL 12 sebanyak padat tebar 2.000 ekor/m2 (A), 3.000 ekor/m2 (B), dan 4.000 ekor/m2 (C) masing-masing 3 kali ulangan. Hasil penelitian menunjukkan bahwa pertumbuhan tokolan pada perlakuan 2.000 dan 3.000 ekor/m2 tidak berbeda nyata (0,57—0,65 g/ekor) (P>0,05), tetapi berbeda nyata pada padat penebaran 4.000 ekor/m2 (0,39 g/ekor) (P<0,05). Demikian pula hasil sintasan tampak tidak berbeda nyata pada padat penebaran 2.000 dan 3.000 ekor/m2 (56,8%—66%), tetapi menunjukkan perbedaan nyata pada padat penebaran 4.000 ekor/m2 (45,1%). Karena itu, padat penebaran maksimal benur PL 12 adalah 3.000 ekor/m2 untuk pendederan dalam KJA di laut.Juvenile production of tiger shrimps of post larvae was conducted in floating net cages in Labuange Bay, Barru Regency from May - July 2002. The aim of this research is to know the best stocking density of tiger shrimp post larvae in nursery rearing of floating net cages in the sea. The treatments were stocking density PL 12 (2,000 psc/m2; 3,000 psc/m2; and 4,000 psc/m2), with three replicates each. The results showed that growth of juvenile shrimps was not significantly different between stocking density 2,000 pcs/m2 and 3,000 pcs/m2 (0.57--0.65 g/pcs) (P>0.05), but significantly different with stocking density of 4,000 pcs/m2 (P<0.05). Nevertheless average survival rate of juvenile shrimps was not significant on stocking density 2,000 pcs and 3,000 pcs/m2, but significant different with stocking density of 4,000 pcs/me.Therefore, for juvenill production in floating net cages, the best (maximum) stocking density of potensial 12 is 3,000 pcs/m2.


2020 ◽  
Vol 67 (4) ◽  
Author(s):  
N. Felix ◽  
P. Kalaimani Arasi ◽  
K. Manikandan

A 45-days feeding trial was conducted on juvenile tiger shrimps Penaeus monodon of size 0.997±0.098 g with diets containing Mannan oligosaccharides (Mos) at three graded levels (0.1, 0.2 and 0.3%). Maximum weight gain (1.353 g) was achieved in shrimps fed with 0.3% Mos concentration, followed by 0.2% Mos (1.224 g). Best feed conversion ratio (FCR) of 2.406 was registered in 0.3% Mos groups. Results indicate that there was a significant difference (p<0.01) in weight gain and FCR among the treatments. However, no statistical differences were observed on the specific growth rate (SGR), mean feed intake and survival rate. Feeding trial was followed by a 10 days challenge study with Vibrio harveyi by intramuscular injection and white spot syndrome virus (WSSV) by feeding WSSV infected tissues. Significantly higher survival rate was observed in the shrimp groups fed with 0.3% Mos (66.67%) than the other treatments when challenged with V. harveyi. Tiger shrimps fed with 0.3% Mos challenged with WSSV showed a significantly higher survival rate (66.67%), followed by 0.2% Mos (50%), 0.1% Mos (25%) and control (25%) on day 5 post-challenge. However On day 10 post-challenge with WSSV, except in the groups fed with 0.3% Mos (16.67% survival), 100% mortality was noticed in all other diet groups. Therefore, it is concluded that dietary administration of Mos at 0.3% would enhance the growth and survival of tiger shrimp against V. harveyi. With respect to WSSV challenge, it appears that, though Mos at 0.3% had a positive effect on the shrimp, higher survival was not observed. Whether additional increment in Mos supplementation in the diet would positively affect the survival of tiger shrimps post-WSSV challenge, needs to be investigated further.


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