Exploration of Methanol Utilizing Microbes From Soil and Dung, as Source of Single Cell Protein

The objectives of this research were to explore methanol utilizing microbes by isolation. selection and collection ofmethanol utilizing microbes from soil and dung. further it will be produced as single cell protein.The experiment covers two steps. i.e. I) Sampling of soil and dungfrom Bogor. Cianjur and Karawang. 2) Laboratoryactivity. consisted of: isolation. selection. identification and collection. The microbes were isolated by using the medium ofTani. et al (/982) and its modification. whereas the methanol utilizing microbes were identified by using standard method ofBergeys Manual of Determinative Bacteriology edition Ijh (/994) and Balow et al. (/99 I). The microbes were then proliferatedby using Medium of Mimura et al. (1978) and its modification. Furthermore the cell were harvested and measured itsnitrogen content. The collection of methanol utilizing microbes was conducted with the standard procedure of soilmicrobiology.This research indicated that from 72 samples of soil and dung were obtained 56 isolates of methanol utilizing microbesthat diverse in number and types. Most of methanol utili:ing microbes were isolated from soil and dung by methanol medium 1% and /.5 % in pH 5 and 7. From 56 isolates were chosen 12 isolates to be identified and made as a data base. and then upt as culture collection at Indonesian Center for Biodiversity and Biotechnology (ICBB). Laboratory of Microbiology and Environmental Biotechnology PPLH. and Laboratory of Soil Biology. IPB. From the 12 isolates. 2 isolates were classified into methylotrophic group and they have big potency to be exploited in producing single cell protein. i.e. MelhylococCJIS capsulatus and Acidomonas methanolica. Between 2 isolates. Methylococcus capsulatus (T2M 1 P 1 Cianjur) have a big potency to be used as a source of single cell protein. due to their high content of protein. i.e. 6.4%. Both of the microbes were not pathogenic for human and animal.

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Heterotrophic bacteria growing in association with Methylococcus capsulatus (Bath) in a single cell protein production process

Applied Microbiology and Biotechnology ◽

10.1007/s00253-002-0964-1 ◽

2002 ◽

Vol 59 (1) ◽

pp. 33-39 ◽

Cited By ~ 54

Author(s):

Harald Bothe ◽

K. Møller Jensen ◽

Mergel A. ◽

Larsen J. ◽

Jørgensen C. ◽

...

Keyword(s):

Production Process ◽

Single Cell ◽

Protein Production ◽

Heterotrophic Bacteria ◽

Single Cell Protein ◽

Cell Protein ◽

Methylococcus Capsulatus ◽

Protein Production Process ◽

Single Cell Protein Production

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Single Cell Protein (Scp) Production from Marine Lactobacillus spp.

Legume Research - An International Journal ◽

10.18805/ijar.b-3858 ◽

2020 ◽

Author(s):

A. Rathipriya ◽

B. Kannan ◽

A. Dhinakaran ◽

A. Hema

Keyword(s):

Single Cell ◽

Tamil Nadu ◽

Fish Meal ◽

Standard Procedure ◽

Single Cell Protein ◽

Lactobacillus Fermentum ◽

Diffusion Method ◽

Cell Protein ◽

Scale Production ◽

Single Strain

Background: The Single Cell Protein (SCP) production technologies arose as a promising way to solve the problem of protein shortage in worldwide, which mainly used in human foods or animal feeds. For future success, the animal proteins are expensive protein source in fish feeds and there has been considerable interest in replacing all or part of the fish meal in aquaculture feeds with SCPs. Collectively, studies indicated that SCPs were suitable ingredients for farmed fishes. The Lactobacillus fermentum strain used in this study was surprising to note a single strain contains 20 amino acids. In this study the strain used and seemed to be ideal in the above respects. Further research on industrial scale production of SCP using this strain using cheaper sources (or) sewage is warranted. Methods: In this field – laboratory investigation during 2015-2016, the soil samples were collected from the Velar estuary, Tamil Nadu. About 25 colonies were selected and inoculated in to MRS broth. The completed preparation was observed under microscope for motility of the bacterium. Different biochemical characteristics test analyzed by using standard protocol. The antimicrobial effects were determined by the agar diffusion method. Finally the biochemical parameters were analyzed by using standard procedure. Result: Our investigation is evident that, the strain very well can be used a probiotic for human and animal nutrition. The isolated strains were found to be non-motile and non-spore farming. Lactobacillus fermentum contains 60-80% of protein, 7-8% of carbohydrate, 2-3% of lipid and 8-9% of nucleic acid contents. The Lactobacillus fermentum strain used in this study showed a high level of inhibitory activity against all the pathogens tested. SCP cannot compete with soya, alfalfa or fish meal. Mushroom production from lignocellulosics seems to be one economical and promising use for SCP.

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