scholarly journals Evaluation of Yield and Pigment Content of Eleven Genotypes of Green and Purple Pod Yard Long Bean (Vigna unguiculata (L.) Walp.)

2019 ◽  
Vol 6 (01) ◽  
pp. 30-40
Author(s):  
Okky Tiara Sari Dewi ◽  
Muhamad Syukur

The consumption of yard long bean in Indonesia is high and it has been increasing continuously, but the production and harvest area has been decreasing. New, superior long bean varieties with higher productivity are required to meet the increasing demands. The aim of this study was to evaluate the morphological and yield characters of selected purple and green long bean genotypes. The research was conducted from February to May 2018 in the Madiun district, East Java, Indonesia. The experiment was laid out in a randomized complete block design with genotypes as the single factor treatment, replicated three times. Eleven yard long bean genotypes were tested, consisting of four new genotypes, F7-013014- 4U-16-1-1, “F7-013014-4U-16-1-2”, “F7-013014- 4U-16-1-3”, and “F7-013014-7P-4-1-1”, and seven control genotypes, “KP13”, “KP14”, “KP Putih China”, “KP Putih China”, “Borneo”, “Sabrina” and “Parade”. The new genotypes, “F7-013014-4U-16-1-1, “F7- 013014-4U-16-1-2”, “F7-013014-4U-16-1-3”, and “F7-013014-7P-4-1-1” had at least one superior characters compared to the control genotypes, i.e. earlier fl owering and time to harvest, longer leaves, greater pod weight, longer pods, larger pod diameter, and higher contents of anthocyanin, carotene, chlorophyll a, chlorophyll b, and total chlorophyll. Keywords: anthocyanin, genotype, pigment, carotene, chlorophyll

2020 ◽  
Vol 8 (2) ◽  
pp. 234
Author(s):  
Made Hary Sayoga ◽  
Ni Made Wartini ◽  
Lutfi Suhendra

 Fragrant pandan leaves can be used as a natural coloring because they contain chlorophyll obtained through the extraction process. The purposes of this study were to determine the effect of particle size and extraction time on the characteristics of natural pandanus fragrance leaf extract and determine the best treatment of particle size and extraction time to produce natural pandanus leaf extract dye. This study used a 2 factorial factorial randomized block design. The first factor is the particle size which consists of 3 levels, namely 40, 60, 80 mesh. The second factor is the extraction time which consists of 3 levels, namely 24, 36, and 48 hours. The results showed that the treatment of particle size and extraction time affected the yield, chlorophyll a, chlorophyll b, total chlorophyll, brightness (L *), redness (a *) and yellowish level (b *). The treatment of 60 mesh particle size and 36 hour extraction time are the best treatment to produce natural pandanus fragrance extract coloring with yield characteristics of 8.81% chlorophyll a level of 23.66%, chlorophyll b level of 3.92%, total chlorophyll content of 27.57%, the brightness level (L *) 21.06, the redness level (a *) 4.92, the yellowish level (b *) 6.21. Keywords: Pandanus amaryllifolius R., chlorophyll, color, extraction


2019 ◽  
Vol 7 (4) ◽  
pp. 571
Author(s):  
I Made Dwipayana ◽  
Ni Made Wartini ◽  
Luh Putu Wrasiati

Dyes from fragrant pandan leaves contain chlorophyll obtained through the extraction process. The purpose of this study were to determine the effect of the ratio of ingredients to solvents and the length of extraction on the characteristics of natural coloring extracts of fragrant pandan leaves and determine the best treatment from the comparison of ingredients with solvents and extraction time to produce natural coloring extracts of fragrant pandan leaves. This study used a 2 factor factorial randomized block design. The first factor is the comparison of materials with solvents consisting of 3 levels, namely 1:13, 1:15, and 1:17. The results showed that the comparative treatment of the material with the solvent and the extraction time greatly influenced the extract yield, chlorophyll a levels, chlorophyll b levels, total chlorophyll levels, brightness level L *, redness level a *, yellowish level b *, interactions greatly influenced the levels chlorophyll a, chlorophyll b level, total chlorophyll content, redness level (a *), yellowish level (b *) but have no effect on extract yield and brightness level (L *). Comparison of material treatment with solvent 1:15 and extraction time for 36 hours, is the best treatment to produce pandanus fragrant leaf extract coloring with extract yield characteristics of 10.32%, chlorophyll a levels of 3402.04 ppm, chlorophyll b levels of 612.23 ppm, total chlorophyll content of 4013.42 ppm, brightness level (L *) 35.27, redness level (a *) 17.15, yellowish level (b *) 30.43. Keywords: Pandanus amaryllifolius Roxb., chlorophyll, nature color, extraction.


2021 ◽  
Vol 9 (1) ◽  
pp. 53
Author(s):  
Rian Lamhot Parasian Hutabarat ◽  
Ni Made Wartini ◽  
Nyoman Semadi Antara

This study aims to determine the effect of type of solvent and maceration temperature on the characteristics of cassava leaves extracts as a natural dyes leaves and determine the type of solvent for cassava leaf powder and the best maceration temperature to obtain the extract of natural cassava leaves. This study used a factorial randomized block design with 2 factors, namely the type of solvent (85% ethanol, 85% acetone and mixture (85% ethanol: 85% acetone) and maceration temperature (35 ° C, 45 ° C and 55 ° C). The data obtained were analyzed with variance and if the treatment had a significant effect, then continued with the Tukey test. The results showed that the treatment interactions had a very significant effect on yield, chlorophyll b, brightness (L *), yellowish level (b *), total chlorophyll, chlorophyll a. , redness (a *), but had no significant effect on the interaction at the level of redness (a *). The treatment of 85% acetone solvent and 55 ° C maceration temperature was the best treatment to produce acetone extract of natural dye of cassava leaves with yield characteristics of 14, 04%, total chlorophyll 12.10%, chlorophyll a 4.39%, chlorophyll b 7.71%, brightness (L *) 16.46, redness (a *) 9.77 yellowish (b *) of 5.70.brightness level (L*) of 11.03, redness level (a*) of 10.29, yellowish level (b*) of 2.83. Key words: cassava leaves, dye extract, maceration temperature, natural dye, solvent type.


2019 ◽  
Vol 47 (1) ◽  
pp. 61-67
Author(s):  
Helvi Ardana Reswari ◽  
Muhamad Syukur ◽  
Dan Willy Bayuardi Suwarno

Breeding improved yardlong bean varieties is not only aimed for yield, but also for enhancing nutritional content such as anthocyanin and carotenoids concentrations. The objectives of this research were to elucidate the anthocyanin and carotenoids concentrations as well as yield components in several yard long bean genotypes. This research was conducted at the Leuwikopo Experimental Station and Post Harvest Laboratory of Department of Agronomy and Horticulture IPB from November 2017 to January 2018. The experiment was arranged in a single-factor randomized complete block design with 4 replicates, involving 15 yardlong bean genotypes. The results showed that Fagiola 2 had relatively high anthocyanin and carotenoids contents. Peleton had higher yield than Kinaya 1, Pangeran Anvi, Parade, and Fagiola 2. Anthocyanin content was not correlated with yield components, whereas carotenoids content was positively correlated with the length of pod stalks, length of pods, weight per pod, chlorophyll a, chlorophyll b, and total chlorophyll. Pod weights per plant were positively correlated with the number of pods per plant (r = 0.81, P<0.01), and can be predicted by a linear model involving number of pods per plant and days to harvest (R2 = 0.75, P<0.01).Keywords: biofortification, chlorophyll, correlation, stepwise regression, Vigna unguiculata var. sesquipedalis


Author(s):  
Wawan Sulistiono ◽  
Abubakar Ibrahim ◽  
Bayu Suwitono ◽  
Himawan Bayu Aji ◽  
Abdul Wahab ◽  
...  

Aims: The objective of this study was to determine the optimal fertilizing method and NPK fertilizer dosage for plant physiology and nutmeg yield. Study Design: The experimental design was a completely randomized block design (CRBD) in a 4x3 factorial arrangement with three replicates. The first factor was four NPK fertilizer doses: 1, 1.5, 2.0, and 2.5kg NPK (15:15:15)/tree/year. The second factor was the manner of fertilizer application: (1) fertilized in spot, (2) fertilized in-furrow trenches trunked and (3) fertilized in hole. Place and Duration of Study: The research was conducted in Tobelo North Halmahera (1°44'45.3"-1°44'54.4" N, 127°59'54.4"-127°59'53.2" E), North Maluku, Indonesia from January 2019 to May 2020. Methodology: The nutmeg trees used was 30 years old. The population of trees plants in the study area was 40 trees. Observation parameters on leaves are chlorophyll a, b and total, nitrate reductase activity (NRA), N, P, K, proline content, number of young and old fruits. Results: The fertilization treatment with the fertilizer application method into the crack/hole fertilizer with a dose of 2.5 kg NPK significantly produced high levels of chlorophyll a, chlorophyll b,         total chlorophyll of leaves and old fruit. The number of old fruits is positively correlated with chlorophyll a content (0.615*), chlorophyll b (0.663*), total chlorophyll (0.634*) and number of young fruits (0.748 **). The application of fertilizer hole produces plants that recover quickly from drought stress, as evidenced by the highest proline content. Conclusion: NPK fertilization using the hole method and at doses of 2.0-2.5 kg/tree/year are recommended for improving plant physiology and yield.


2020 ◽  
Vol 5 (2) ◽  
pp. 49
Author(s):  
Ezra Elkana Karo Sekali ◽  
Ni Made Wartini ◽  
Lutfi Suhendra

Penelitian ini bertujuan untuk  mengetahui pengaruh ukuran partikel dan lama maserasi terhadap karakteristik ekstrak aseton pewarna alami daun singkong dan menentukan ukuran partikel dan lama maserasi terbaik untuk mendapatkan ekstrak aseton pewarna alami daun singkong. Penelitian ini menggunakan Rancangan Acak Kelompok Pola Faktorial dengan 2 Faktor yaitu Ukuran Partikel (40 mesh, 60 mesh, 80 mesh) dan Lama Maserasi (24 jam, 36 jam, 48 jam). Data yang diperoleh dianalisis dengan sidik ragam dan apabila perlakuan berpengaruh nyata dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa interaksi perlakuan berpengaruh sangat nyata terhadap rendemen, klorofil b, tingkat kecerahan (L*), tingkat kekuningan (b*) tetapi berpengaruh tidak nyata terhadap klorofil total, klorofil a, kemerahan (a*). Perlakuan ukuran partikel 80 mesh dan lama maserasi 36 jam merupakan perlakuan terbaik untuk menghasilkan ekstrak aseton pewarna alami daun singkong dengan karakteristik rendemen sebesar 8,08%, klorofil total sebesar 3,83%, klorofil a sebesar 2,05%, klorofil b sebesar 1,77%, tingkat kecerahan (L*) sebesar 11,03, tingkat kemerahan (a*) sebesar 10,29, tingkat kekuningan (b*) sebesar 2,83.   This study aims to know the effect of particle size and maceration time on the characteristics of the natural dye acetone extract of cassava leaves and determine the best particle size and maceration time to obtain the acetone extract of natural coloring cassava leaves.This research used a randomized block design with factorial pattern with two factors namely, particle size (40 mesh, 60 mesh, 80 mesh) and maceration time (24 hours, 36 hours, 48 hours). The data obtained were analyzed by analysis of variance. If the treatment had a significant effect, it would be followed by the Duncan test. The result showed that interactions had significant effect on theextract yield, chlorophyll b levels, brightness level L*, yellowish level b*, but had no effect on total chlorophyll levels, chlorophyll a levels, redness level a*. Treatment of particle size 80 mesh and maceration time 36 hours, is the best treatment to produce aceton extract natural dyes of cassava leaves with extract yield characteristics of 8.08%,total chlorophyll levels of 3.83, chlorophyll a levels of 2.05%,chlorophyll b levels of 1.77%,brightness level (L*) of 11.03,redness level (a*) of 10.29,yellowish level (b*) of 2.83.


1973 ◽  
Vol 37 ◽  
Author(s):  
N. Lust

Pigment content of ashes grown up under different circumstances - The pigment content (chlorophyll a, chlorophyll b,  xanthophyll and carotene) has been researched with ashes grown up under  different light circumstances and varying in age and height.     The results prove that the general laws concerning the influence of light  on the pigment content, don’t always work.     The phenomen is very complex. The light quantity is very important in some  cases, but insignificant in others. It seems origin and height of plants have  a strong influence. The results prove also the influence of the environment  is much higher on small plants as on big ones.     The research indicates finally the correlation between the green pigments,  the yellow pigments, and between the green pigments on the one side and the  yellow ones on the other side.


2020 ◽  
Vol 47 (3) ◽  
pp. 326-332
Author(s):  
Syahdin Launuru ◽  
Ade Wachjar ◽  
Dan Ani Kurniawati

Application of right dosage of fertilizer and concentration of plant growth regulator are supporting factor of clove growth. This study was aimed to obtain the best combination of organic-inorganic fertilizer, the best triacontanol concentration and both interactions for increasing clove growth. This study used a Randomized Complete Block Design (RCBD) with two factors. The first factor was the organic-inorganic fertilizer dosage package that consisted of 6 levels, i.e no fertilizer, 100% recommended dosage of inorganic fertilizer (r.d.i.f), 25% recommended dosage of organic fertilizer (r.d.o.f) + 75% r.d.i.f, 50% r.d.o.f + 50% r.d.i.f, 75% r.d.o.f + 25% r.d.i.f, 100% r.d.o.f. The second factor was the concentration of triacontanol that consisted of 3 levels, i.e no triacontanol, 2 mg L-1 and 4 mg L-1. The results showed that the application of 25% r.d.o.f + 75% r.d.i.f produced the best clove growth, but it was not significantly different from the combination treatment of the two other types of fertilizer as indicated by the greatest improvement on stem diameter, branches number, leaf area, chlorophyll a, chlorophyll b, total chlorophyll and carotenoids. Clove plants did not respond to the application of triacontanol at a concentration of 2 mg L-1, but gave a negative response to the application of triacontanol 4 mg L-1 on the plant height and leaf area variables. The interaction of the two factors did not have significant effects on all variables. Keywords: chlorophyll, morphology, nutrient dynamics, physiology, vegetative growth  


2020 ◽  
Vol 33 (02) ◽  
pp. 694-706
Author(s):  
Hassan Sarhadi ◽  
Jahanfar Daneshian ◽  
Seyyed Alireza Valadabadi ◽  
Hossein Heidary Sharafabad ◽  
Hossein Heidary Sharafabad ◽  
...  

The response of active ingredient, chlorophyll a and b contents, and chlorophyll index of henna to different rates of N fertilization and drought stress was explored in a split-split-plot experiment based on a randomized complete block design with four replications in the research farm of Jiroft Branch, Islamic Azad University, Jiroft, Iran in the 2016-2017 growing season. In the current study, the main plot was assigned to drought stress at three levels (including irrigation to supply 100%, 75% or 50% of plant water requirement), the sub-plot was assigned to N fertilization at three rates (50, 100 or 150 kg ha-1 pure N), and the sub-sub-plot was assigned to ecotype at three levels (Bami, Bushehri, and Rudbari). The results showed that the effect of drought level was significant on active ingredient and chlorophyll index at the p < 0.01 level and on chlorophyll a, total chlorophyll, and chlorophyll a/b ratio at the p < 0.05 level.  But, it could not influence chlorophyll b significantly. The effect of N fertilization was significant on most studied traits at the p< 0.01 level, but its effect was significant on the chlorophyll index at the p < 0.05 level and insignificant on dye and chlorophyll b. According to the results, it seems that the application of 100 kg ha-1 N and the irrigation to supply 75% of plant water requirement can contribute to having more fresh plants with the higher active ingredient and dye contents.


Author(s):  
Maria Sastriana ◽  
Ngadiani Ngadiani

The aim of this study was to compare the organic fertilizer of azolla pinnata and banana peel and a combination of azolla pinnata and banana peel with the same concentration of 800 ppm to the weight of harvest and the chlorophyll content of ground spinach (Ipomoea reptans Poir.). The experiment was carried out experimentally using a completely randomized design with control treatment, 800 ppm azolla pinnata, 800 ppm banana skin and a combination of azolla pinnata and banana peels 800 ppm which were repeated 6 times each. The application was carried out by pouring 800 ppm of azolla pinnata, 800 ppm of banana peel and a combination of azolla pinnata and 800 ppm banana peel on each gutter. The results showed that azolla pinnata and banana peel nutrition had a significant effect (P <0.05) on harvest weight and chlorophyll content of ground spinach plants (Ipomoea reptans Poir.). Nutrition azolla pinnata and banana peel with a concentration of 800 ppm can increase the weight of the land spinach (Ipomoea reptans Poir.) Which has the highest average. In testing the chlorophyll content, the results of chlorophyll a content, chlorophyll b content and the highest total chlorophyll content was found in azolla pinnata 800 ppm nutrition. The results of this study concluded that 800 ppm azolla pinnata, 800 ppm banana peel and 800 ppm combination of azolla pinnata and banana peel in the growth of ground spinach plants (Ipomoea reptans Poir.) Were effective to increase harvest weight and increase chlorophyll content of land spinach (Ipomoea reptans Poir.) Namely chlorophyll a, chlorophyll b and total chlorophyll of land spinach plants (Ipomoea reptans Poir.).   Keywords: ground kale, azolla pinnata, banana peel, harvest weight, test of chlorophyll content, chlorophyll a, chlorophyll b, total chlorophyll.


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