scholarly journals Effect of NPK Fertilization Method on the Physiology and Fruit Products of Myristica fragrans in North Maluku, Indonesia

2021 ◽  
pp. 19-29
Author(s):  
Wawan Sulistiono ◽  
Abubakar Ibrahim ◽  
Bayu Suwitono ◽  
Himawan Bayu Aji ◽  
Abdul Wahab ◽  
...  
Keyword(s):  
Plant Physiology ◽  
Chlorophyll A ◽  
Reductase Activity ◽  
Block Design ◽  
Fertilizer Application ◽  
Proline Content ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Npk Fertilizer ◽  
Npk Fertilization

Aims: The objective of this study was to determine the optimal fertilizing method and NPK fertilizer dosage for plant physiology and nutmeg yield. Study Design: The experimental design was a completely randomized block design (CRBD) in a 4x3 factorial arrangement with three replicates. The first factor was four NPK fertilizer doses: 1, 1.5, 2.0, and 2.5kg NPK (15:15:15)/tree/year. The second factor was the manner of fertilizer application: (1) fertilized in spot, (2) fertilized in-furrow trenches trunked and (3) fertilized in hole. Place and Duration of Study: The research was conducted in Tobelo North Halmahera (1°44'45.3"-1°44'54.4" N, 127°59'54.4"-127°59'53.2" E), North Maluku, Indonesia from January 2019 to May 2020. Methodology: The nutmeg trees used was 30 years old. The population of trees plants in the study area was 40 trees. Observation parameters on leaves are chlorophyll a, b and total, nitrate reductase activity (NRA), N, P, K, proline content, number of young and old fruits. Results: The fertilization treatment with the fertilizer application method into the crack/hole fertilizer with a dose of 2.5 kg NPK significantly produced high levels of chlorophyll a, chlorophyll b,         total chlorophyll of leaves and old fruit. The number of old fruits is positively correlated with chlorophyll a content (0.615*), chlorophyll b (0.663*), total chlorophyll (0.634*) and number of young fruits (0.748 **). The application of fertilizer hole produces plants that recover quickly from drought stress, as evidenced by the highest proline content. Conclusion: NPK fertilization using the hole method and at doses of 2.0-2.5 kg/tree/year are recommended for improving plant physiology and yield.

scholarly journals Sorbitol-induced changes in various growth and biochemici parameters in maize

2010 ◽  
Vol 56 (No. 6) ◽  
pp. 263-267 ◽  
Author(s):  
M. Jain ◽  
S. Tiwary ◽  
R. Gadre
Keyword(s):  
Nitrate Reductase ◽  
Chlorophyll A ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Proline Content ◽  
Shoot Length ◽  
Carotenoid Content ◽  
Dependent Manner ◽  
Chlorophyll B ◽  
Maize Seedlings

Treatment of maize seedlings with different concentrations of sorbitol decreased the rate of germination substantially. Root and shoot length was also reduced by sorbitol treatment, however, decrease in root length was lower than shoot length. Incubation of leaf segments from maize seedlings grown in continuous light with sorbitol decreased the fresh weight and increased the dry weight in a concentration-dependent manner. Sorbitol treatment also reduced the total chlorophylls, chlorophyll a as well as chlorophyll b; the decrease in chlorophyll 'b' being more prominent than chlorophyll 'a', however, carotenoid content was declined marginally. Supply of sorbitol decreased the protein and RNA content; however, proline content and in vivo nitrate reductase activity (NRA) were increased. The results demonstrate an inhibitory effect of sorbitol-induced stress on overall growth in maize. Amongst the biochemical parameters analysed, chlorophyll, protein and RNA contents were declined, while proline content and nitrate reductase activity were enhanced with sorbitol treatment.

scholarly journals Pengaruh Ukuran Partikel dan Lama Ekstraksi terhadap Karakteristik Ekstrak Pewarna Alami Daun Pandan Wangi (Pandanus amaryllifolius R.)

2020 ◽  
Vol 8 (2) ◽  
pp. 234
Author(s):  
Made Hary Sayoga ◽  
Ni Made Wartini ◽  
Lutfi Suhendra
Keyword(s):  
Particle Size ◽  
Chlorophyll A ◽  
Leaf Extract ◽  
Block Design ◽  
Extraction Process ◽  
Extraction Time ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Randomized Block Design ◽  
Pandanus Amaryllifolius

 Fragrant pandan leaves can be used as a natural coloring because they contain chlorophyll obtained through the extraction process. The purposes of this study were to determine the effect of particle size and extraction time on the characteristics of natural pandanus fragrance leaf extract and determine the best treatment of particle size and extraction time to produce natural pandanus leaf extract dye. This study used a 2 factorial factorial randomized block design. The first factor is the particle size which consists of 3 levels, namely 40, 60, 80 mesh. The second factor is the extraction time which consists of 3 levels, namely 24, 36, and 48 hours. The results showed that the treatment of particle size and extraction time affected the yield, chlorophyll a, chlorophyll b, total chlorophyll, brightness (L *), redness (a *) and yellowish level (b *). The treatment of 60 mesh particle size and 36 hour extraction time are the best treatment to produce natural pandanus fragrance extract coloring with yield characteristics of 8.81% chlorophyll a level of 23.66%, chlorophyll b level of 3.92%, total chlorophyll content of 27.57%, the brightness level (L *) 21.06, the redness level (a *) 4.92, the yellowish level (b *) 6.21. Keywords: Pandanus amaryllifolius R., chlorophyll, color, extraction

scholarly journals Pengaruh Perbandingan Bahan Pelarut dan Lama Ekstraksi terhadap Karakteristik Ekstrak Pewarna Daun Pandan Wangi (Pandanus amaryllifolius Roxb.)

2019 ◽  
Vol 7 (4) ◽  
pp. 571
Author(s):  
I Made Dwipayana ◽  
Ni Made Wartini ◽  
Luh Putu Wrasiati
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Block Design ◽  
Extraction Time ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
Brightness Level ◽  
Extract Yield ◽  
Pandanus Amaryllifolius

Dyes from fragrant pandan leaves contain chlorophyll obtained through the extraction process. The purpose of this study were to determine the effect of the ratio of ingredients to solvents and the length of extraction on the characteristics of natural coloring extracts of fragrant pandan leaves and determine the best treatment from the comparison of ingredients with solvents and extraction time to produce natural coloring extracts of fragrant pandan leaves. This study used a 2 factor factorial randomized block design. The first factor is the comparison of materials with solvents consisting of 3 levels, namely 1:13, 1:15, and 1:17. The results showed that the comparative treatment of the material with the solvent and the extraction time greatly influenced the extract yield, chlorophyll a levels, chlorophyll b levels, total chlorophyll levels, brightness level L *, redness level a *, yellowish level b *, interactions greatly influenced the levels chlorophyll a, chlorophyll b level, total chlorophyll content, redness level (a *), yellowish level (b *) but have no effect on extract yield and brightness level (L *). Comparison of material treatment with solvent 1:15 and extraction time for 36 hours, is the best treatment to produce pandanus fragrant leaf extract coloring with extract yield characteristics of 10.32%, chlorophyll a levels of 3402.04 ppm, chlorophyll b levels of 612.23 ppm, total chlorophyll content of 4013.42 ppm, brightness level (L *) 35.27, redness level (a *) 17.15, yellowish level (b *) 30.43. Keywords: Pandanus amaryllifolius Roxb., chlorophyll, nature color, extraction.

scholarly journals Karakteristik Ekstrak Pewarna Alami Daun Singkong (Manihot esculenta) pada Perlakuan Jenis Pelarut dan Suhu Maserasi

2021 ◽  
Vol 9 (1) ◽  
pp. 53
Author(s):  
Rian Lamhot Parasian Hutabarat ◽  
Ni Made Wartini ◽  
Nyoman Semadi Antara
Keyword(s):  
Chlorophyll A ◽  
Block Design ◽  
Natural Dye ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Cassava Leaves ◽  
Randomized Block Design ◽  
Solvent Type ◽  
Leaf Powder ◽  
Effect On Yield

This study aims to determine the effect of type of solvent and maceration temperature on the characteristics of cassava leaves extracts as a natural dyes leaves and determine the type of solvent for cassava leaf powder and the best maceration temperature to obtain the extract of natural cassava leaves. This study used a factorial randomized block design with 2 factors, namely the type of solvent (85% ethanol, 85% acetone and mixture (85% ethanol: 85% acetone) and maceration temperature (35 ° C, 45 ° C and 55 ° C). The data obtained were analyzed with variance and if the treatment had a significant effect, then continued with the Tukey test. The results showed that the treatment interactions had a very significant effect on yield, chlorophyll b, brightness (L *), yellowish level (b *), total chlorophyll, chlorophyll a. , redness (a *), but had no significant effect on the interaction at the level of redness (a *). The treatment of 85% acetone solvent and 55 ° C maceration temperature was the best treatment to produce acetone extract of natural dye of cassava leaves with yield characteristics of 14, 04%, total chlorophyll 12.10%, chlorophyll a 4.39%, chlorophyll b 7.71%, brightness (L *) 16.46, redness (a *) 9.77 yellowish (b *) of 5.70.brightness level (L*) of 11.03, redness level (a*) of 10.29, yellowish level (b*) of 2.83. Key words: cassava leaves, dye extract, maceration temperature, natural dye, solvent type.

scholarly journals Evaluation of Yield and Pigment Content of Eleven Genotypes of Green and Purple Pod Yard Long Bean (Vigna unguiculata (L.) Walp.)

2019 ◽  
Vol 6 (01) ◽  
pp. 30-40
Author(s):  
Okky Tiara Sari Dewi ◽  
Muhamad Syukur
Keyword(s):  
Chlorophyll A ◽  
Vigna Unguiculata ◽  
Block Design ◽  
Pigment Content ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Single Factor ◽  
Randomized Complete Block Design

The consumption of yard long bean in Indonesia is high and it has been increasing continuously, but the production and harvest area has been decreasing. New, superior long bean varieties with higher productivity are required to meet the increasing demands. The aim of this study was to evaluate the morphological and yield characters of selected purple and green long bean genotypes. The research was conducted from February to May 2018 in the Madiun district, East Java, Indonesia. The experiment was laid out in a randomized complete block design with genotypes as the single factor treatment, replicated three times. Eleven yard long bean genotypes were tested, consisting of four new genotypes, F7-013014- 4U-16-1-1, “F7-013014-4U-16-1-2”, “F7-013014- 4U-16-1-3”, and “F7-013014-7P-4-1-1”, and seven control genotypes, “KP13”, “KP14”, “KP Putih China”, “KP Putih China”, “Borneo”, “Sabrina” and “Parade”. The new genotypes, “F7-013014-4U-16-1-1, “F7- 013014-4U-16-1-2”, “F7-013014-4U-16-1-3”, and “F7-013014-7P-4-1-1” had at least one superior characters compared to the control genotypes, i.e. earlier fl owering and time to harvest, longer leaves, greater pod weight, longer pods, larger pod diameter, and higher contents of anthocyanin, carotene, chlorophyll a, chlorophyll b, and total chlorophyll. Keywords: anthocyanin, genotype, pigment, carotene, chlorophyll

scholarly journals Karakteristik Ekstrak Aseton Pewarna Alami Daun Singkong (Manihot Esculenta C.) pada Perlakuan Ukuran Partikel Bahan dan Lama Maserasi

2020 ◽  
Vol 5 (2) ◽  
pp. 49
Author(s):  
Ezra Elkana Karo Sekali ◽  
Ni Made Wartini ◽  
Lutfi Suhendra
Keyword(s):  
Particle Size ◽  
Chlorophyll A ◽  
Block Design ◽  
Acetone Extract ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Brightness Level ◽  
Cassava Leaves ◽  
Randomized Block Design ◽  
Two Factors

Penelitian ini bertujuan untuk  mengetahui pengaruh ukuran partikel dan lama maserasi terhadap karakteristik ekstrak aseton pewarna alami daun singkong dan menentukan ukuran partikel dan lama maserasi terbaik untuk mendapatkan ekstrak aseton pewarna alami daun singkong. Penelitian ini menggunakan Rancangan Acak Kelompok Pola Faktorial dengan 2 Faktor yaitu Ukuran Partikel (40 mesh, 60 mesh, 80 mesh) dan Lama Maserasi (24 jam, 36 jam, 48 jam). Data yang diperoleh dianalisis dengan sidik ragam dan apabila perlakuan berpengaruh nyata dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa interaksi perlakuan berpengaruh sangat nyata terhadap rendemen, klorofil b, tingkat kecerahan (L*), tingkat kekuningan (b*) tetapi berpengaruh tidak nyata terhadap klorofil total, klorofil a, kemerahan (a*). Perlakuan ukuran partikel 80 mesh dan lama maserasi 36 jam merupakan perlakuan terbaik untuk menghasilkan ekstrak aseton pewarna alami daun singkong dengan karakteristik rendemen sebesar 8,08%, klorofil total sebesar 3,83%, klorofil a sebesar 2,05%, klorofil b sebesar 1,77%, tingkat kecerahan (L*) sebesar 11,03, tingkat kemerahan (a*) sebesar 10,29, tingkat kekuningan (b*) sebesar 2,83.   This study aims to know the effect of particle size and maceration time on the characteristics of the natural dye acetone extract of cassava leaves and determine the best particle size and maceration time to obtain the acetone extract of natural coloring cassava leaves.This research used a randomized block design with factorial pattern with two factors namely, particle size (40 mesh, 60 mesh, 80 mesh) and maceration time (24 hours, 36 hours, 48 hours). The data obtained were analyzed by analysis of variance. If the treatment had a significant effect, it would be followed by the Duncan test. The result showed that interactions had significant effect on theextract yield, chlorophyll b levels, brightness level L*, yellowish level b*, but had no effect on total chlorophyll levels, chlorophyll a levels, redness level a*. Treatment of particle size 80 mesh and maceration time 36 hours, is the best treatment to produce aceton extract natural dyes of cassava leaves with extract yield characteristics of 8.08%,total chlorophyll levels of 3.83, chlorophyll a levels of 2.05%,chlorophyll b levels of 1.77%,brightness level (L*) of 11.03,redness level (a*) of 10.29,yellowish level (b*) of 2.83.

scholarly journals The effect of drought stress and nitrogen fertilization on the active ingredient, chlorophyll A and B, and chlorophyll index of henna

2020 ◽  
Vol 33 (02) ◽  
pp. 694-706
Author(s):  
Hassan Sarhadi ◽  
Jahanfar Daneshian ◽  
Seyyed Alireza Valadabadi ◽  
Hossein Heidary Sharafabad ◽  
Hossein Heidary Sharafabad ◽  
...  
Keyword(s):  
Drought Stress ◽  
Chlorophyll A ◽  
Active Ingredient ◽  
Block Design ◽  
N Fertilization ◽  
Water Requirement ◽  
Plant Water ◽  
Chlorophyll B ◽  
Chlorophyll A And B ◽  
Chlorophyll Index

The response of active ingredient, chlorophyll a and b contents, and chlorophyll index of henna to different rates of N fertilization and drought stress was explored in a split-split-plot experiment based on a randomized complete block design with four replications in the research farm of Jiroft Branch, Islamic Azad University, Jiroft, Iran in the 2016-2017 growing season. In the current study, the main plot was assigned to drought stress at three levels (including irrigation to supply 100%, 75% or 50% of plant water requirement), the sub-plot was assigned to N fertilization at three rates (50, 100 or 150 kg ha-1 pure N), and the sub-sub-plot was assigned to ecotype at three levels (Bami, Bushehri, and Rudbari). The results showed that the effect of drought level was significant on active ingredient and chlorophyll index at the p < 0.01 level and on chlorophyll a, total chlorophyll, and chlorophyll a/b ratio at the p < 0.05 level.  But, it could not influence chlorophyll b significantly. The effect of N fertilization was significant on most studied traits at the p< 0.01 level, but its effect was significant on the chlorophyll index at the p < 0.05 level and insignificant on dye and chlorophyll b. According to the results, it seems that the application of 100 kg ha-1 N and the irrigation to supply 75% of plant water requirement can contribute to having more fresh plants with the higher active ingredient and dye contents.

scholarly journals Pemanfaatan Pupuk Organik Kulit Pisang Dan Azolla Terhadap Pertumbuhan Kangkung Darat Dengan Sistem Akuaponik

2019 ◽  
Vol 12 (01) ◽  
pp. 1-10
Author(s):  
Maria Sastriana ◽  
Ngadiani Ngadiani
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Organic Fertilizer ◽  
Control Treatment ◽  
Banana Peel ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Azolla Pinnata ◽  
Randomized Design ◽  
Weight Test

The aim of this study was to compare the organic fertilizer of azolla pinnata and banana peel and a combination of azolla pinnata and banana peel with the same concentration of 800 ppm to the weight of harvest and the chlorophyll content of ground spinach (Ipomoea reptans Poir.). The experiment was carried out experimentally using a completely randomized design with control treatment, 800 ppm azolla pinnata, 800 ppm banana skin and a combination of azolla pinnata and banana peels 800 ppm which were repeated 6 times each. The application was carried out by pouring 800 ppm of azolla pinnata, 800 ppm of banana peel and a combination of azolla pinnata and 800 ppm banana peel on each gutter. The results showed that azolla pinnata and banana peel nutrition had a significant effect (P <0.05) on harvest weight and chlorophyll content of ground spinach plants (Ipomoea reptans Poir.). Nutrition azolla pinnata and banana peel with a concentration of 800 ppm can increase the weight of the land spinach (Ipomoea reptans Poir.) Which has the highest average. In testing the chlorophyll content, the results of chlorophyll a content, chlorophyll b content and the highest total chlorophyll content was found in azolla pinnata 800 ppm nutrition. The results of this study concluded that 800 ppm azolla pinnata, 800 ppm banana peel and 800 ppm combination of azolla pinnata and banana peel in the growth of ground spinach plants (Ipomoea reptans Poir.) Were effective to increase harvest weight and increase chlorophyll content of land spinach (Ipomoea reptans Poir.) Namely chlorophyll a, chlorophyll b and total chlorophyll of land spinach plants (Ipomoea reptans Poir.).   Keywords: ground kale, azolla pinnata, banana peel, harvest weight, test of chlorophyll content, chlorophyll a, chlorophyll b, total chlorophyll.

scholarly journals Biochemical Response of Hybrid Maize (Zea mays L.) to NPK Fertilization Based on Spent Bleaching Earth in Field Scale

2020 ◽  
Vol 142 ◽  
pp. 01004
Author(s):  
Cahya Anugrah ◽  
Didik Indradewa ◽  
Eka Tarwaca Susila Putra
Keyword(s):  
Clay Minerals ◽  
Reductase Activity ◽  
Block Design ◽  
Bleaching Earth ◽  
Oil Quality ◽  
Biochemical Response ◽  
Npk Fertilizers ◽  
Npk Fertilizer ◽  
Hybrid Maize ◽  
Brown Clay

Indonesia is one of the largest crude palm oil (CPO) producing countries in the world. To improve oil quality, CPO purification is carried out including bleaching earth. The process produced spent bleaching earth (SBE) and deoiled spent bleaching earth (DSBE) classified as hazardous and toxic materials waste. SBE and DSBE have the potential as an adhesive material in the manufacture of NPK fertilizers. This study aimed to compare the effects of SBE and DSBE on replacing clay minerals in terms of biochemical response. The study was arranged in randomized completly block design with three treatments and blocks. The treatment consisted of NPK fertilizer containing 10% brown clay (control), NPK fertilizer containing 5% pure clay minerals + 5% SBE, and NPK fertilizer containing 5% pure clay minerals + 5% DSBE. Data were analyzed using ANOVA and tested using LSD test at a 95% confidence level. The results showed that NPK containing DSBE was able to increase nitrate reductase activity (NRA) (41.9%), chlorophyll b content (4.7%), glycine betaine (GB) (2.5%), decreasing electrolite leakage (19%), malondialdehyde (MDA) (23%) compared NPK containing brown clay. Thus the use of NPK containing DSBE and NPK containing SBE to be able to replace NPK containing brown clay.

scholarly journals Salinity stress affects growth and physiology of mulberry (Morus sp.)

2021 ◽  
Vol 948 (1) ◽  
pp. 012049
Author(s):  
Y R E Wulandari ◽  
T Triadiati ◽  
Y C Sulistyaningsih ◽  
A Suprayogi ◽  
M Rahminiwati
Keyword(s):  
Salt Stress ◽  
Plant Height ◽  
Block Design ◽  
Proline Content ◽  
Photosynthesis Rate ◽  
High Salt ◽  
Stem Cuttings ◽  
Total Chlorophyll ◽  
Shoot Number ◽  
High Salt Stress

Abstract Mulberry (Morus sp.) plant is used to feed silkworms, and the leaves contain compounds with medicinal properties of secondary metabolites. However, the content of these compounds tends to increase under stress conditions, for instance, salt stress. This study, therefore, aimed to determine the accessions of mulberry with tolerance for salt stress. The stem cuttings of seven accessions from 5 regions, Bogor, Pati, Situbondo, Bali, and Gowa, were planted following a factorial randomized block design with 3 replications. Subsequently, the first factor using the accessions, and the second factor using NaCl solution (0.0%, 0.2%, 0.3%, and 0.4% concentrations) were performed. The variables observed were growth (leaves number, plant height, and shoots number), photosynthesis rate, total chlorophyll, and proline content. The results showed that the M6 accession exhibited tolerance under high salt stress, based on the leaves number, plant height, shoot number, photosynthesis rate, and proline content. Furthermore, an increase in salt concentration was discovered to cause a decrease in growth, photosynthesis rate, and total chlorophyll content. Also, proline accumulation stimulated by high salt stress possibly plays an important role in salinity tolerance.

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