scholarly journals Saliva as a Non-Invasive Diagnostic Medium in Proteomics for Oral Squamous Cell Carcinoma Detection

2019 ◽  
Vol 6 (3) ◽  
pp. 73-76 ◽  
Author(s):  
Sajad Ahmad Buch ◽  
Laxmikanth Chatra
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Non Invasive

scholarly journals Spectrophotometry of Saliva of Oral Squamous CellCarcinoma Patients

2009 ◽  
Vol 6 (2) ◽  
pp. 373-378
Author(s):  
Baghdad Science Journal
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Cancer ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Ir Spectrum ◽  
Human Saliva ◽  
Test Tube ◽  
Non Invasive ◽  
Back Ground

Back ground: Spectral analyses of solutions have long been applied to various body fluids for the purpose of clinical study as well as research .Human saliva can be easily obtained by non invasive .In this study typical spectra (for UV and IR) of saliva of oral cancer Squamous cell carcinoma patients were determined under average conditions and evaluated in relation to the spectra of normal specimens. Materials and Methods: Seventeen patients of oral cancer Squamous cell carcinoma and seventeen age matched healthy subject were included in this study .Chewing - Stimulated Saliva was collected in plastic test tube and stored at -20° C. Bach of saliva samples were used for UV and IR measurements. Results: Many differences between the IR spectrum of saliva of oral Squamous cell carcinoma patients and IR spectrum of normal saliva. Conclusion: The results in this study were detected that the use of IR spectroscopy may be useful in the diagnosis of oral Squamous cell carcinoma by using saliva samples.

scholarly journals Non-invasive early diagnosis of oral squamous cell carcinoma using piezoelectric biosensor

2020 ◽  
Vol 5 (3) ◽  
pp. 146
Author(s):  
Irene E. Rieuwpassa ◽  
Sumintarti Sumintarti ◽  
Yu-Ri Kim ◽  
Megatriani Matandung ◽  
Gabriel SA. Matongan
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Early Diagnosis ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Non Invasive

scholarly journals Salivary miR-30c-5p as Potential Biomarker for Detection of Oral Squamous Cell Carcinoma

Biomedicines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1079
Author(s):  
Nikolay Mehterov ◽  
Boyan Vladimirov ◽  
Andrea Sacconi ◽  
Claudio Pulito ◽  
Marcin Rucinski ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Target Identification ◽  
Taqman Assay ◽  
Invasive Approach ◽  
Non Invasive ◽  
Bodily Fluids ◽  
Potential Biomarker

The levels of different classes of extracellular RNAs (exRNAs) remain stable in bodily fluids. The detection of either enriched or depleted specific subsets of salivary microRNAs (miRNAs) has the potential to serve as a non-invasive approach for biomarker development. Thus, salivary miRNAs have emerged as a promising molecular tool for early diagnosis and screening of oral squamous cell carcinoma (OSCC). Total RNA was extracted from saliva supernatant of 33 OSCC patients and 12 controls (discovery set), and the differential expression of 8 cancer-related miRNAs was detected by TaqMan assay. Among the screened miRNAs, miR-30c-5p (p < 0.04) was significantly decreased in OSCC saliva. The same transcriptional behavior of miR30c-5p was observed in an additional validation set. miR-30c-5p showed a significant statistical difference between cases and controls with areas under the curve (AUC) of 0.82 (95% CI: 0.71–0.89). The sensitivity and the specificity of miR-30c-5p were 86% and 74%, respectively. The target identification analysis revealed enrichment of miR-30c-5p targets in p53 and Wnt signaling pathways in OSCC. Additionally, the miR-30c-5p targets had clinical significance related to overall survival. In conclusion, these findings show that downregulated miR-30c-5p has the potential to serve as a novel, non-invasive biomarker for early OSCC detection.

scholarly journals Raman difference spectroscopy: a non-invasive method for identification of oral squamous cell carcinoma: publisher’s note

2015 ◽  
Vol 6 (7) ◽  
pp. 2675 ◽  
Author(s):  
Christian Knipfer ◽  
Johanna Motz ◽  
Werner Adler ◽  
Kathrin Brunner ◽  
Medhaine Tesfay Gebrekidan ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Difference Spectroscopy ◽  
Non Invasive ◽  
Invasive Method

scholarly journals Secreted Protein Markers in Oral Squamous Cell Carcinoma (Oscc)

2021 ◽  
Author(s):  
Madiha Mumtaz ◽  
Irene V Bijnsdorp ◽  
Franziska Böttger ◽  
Sander R Piersma ◽  
Thang V Pham ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Protein Localization ◽  
Cancer Cell Line ◽  
P Value ◽  
Protein Markers ◽  
Mortality And Morbidity ◽  
Non Invasive

Abstract Background Oral squamous cell carcinoma (OSCC) is a main cause of oral cancer mortality and morbidity in central south Asia. To improve the clinical outcome of OSCC patients, early detection markers are needed, which are preferably non-invasive and thus independent of a tissue biopsy. Methods In the present study, we aimed to identify robust candidate protein biomarkers for early OSCC diagnosis. To this end, we measured the global protein profiles of OSCC tissue lysates to matched normal adjacent mucosa samples (n=14) and the secretomes of nine HNSCC cell lines using LC-MS/MS-based proteomics. Results A total of 5,123 tissue proteins were identified, of which 205 were robustly up- regulated (p-value <0.01, fold change ˃+2) in OSCC-tissues compared to normal tissues. The biological process “Secretion” was highly enriched in this set of proteins. Other upregulated biological pathways included “Unfolded Protein Response”, “Spliceosomal complex assembly”, “Protein localization to endosome” and “Interferon Gamma Response”. Transcription factor analysis implicated Creb3L1, ESRRA, YY, ELF2, STAT1 and XBP as potential regulators. Of the 205 upregulated tissue proteins, 132 were identified in the cancer cell line secretomes, underscoring their potential use as non-invasive biofluid markers. To further prioritize our candidate markers for non-invasive OSCC detection, we integrated our data with public biofluid datasets including OSCC saliva, yielding 25 candidate markers for further study. Conclusions We identified several key proteins and processes that are associated with OSCC tissues, underscoring the importance of altered secretion. Cancer-associated OSCC secretome proteins present in saliva have potential to be used as novel non-invasive biomarkers for the early diagnosis of OSCC.

scholarly journals Salivary Micro-RNA and Oral Squamous Cell Carcinoma: A Systematic Review

2021 ◽  
Vol 11 (2) ◽  
pp. 101
Author(s):  
Maria Menini ◽  
Emanuele De Giovanni ◽  
Francesco Bagnasco ◽  
Francesca Delucchi ◽  
Francesco Pera ◽  
...  
Keyword(s):  
Systematic Review ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cost Effective ◽  
Clinical Results ◽  
Micro Rna ◽  
Non Invasive ◽  
Cost Effective Method

Oral squamous cell carcinoma (OSCC) is a widespread malignancy with high mortality. In particular, a delay in its diagnosis dramatically decreases the survival rate. The aim of this systematic review was to investigate and summarize clinical results in the literature, regarding the potential use of salivary microRNAs (miRNAs) as diagnostic and prognostic biomarkers for OSCC patients. Twelve papers were selected, including both case–control and cohort studies, and all of them detected significantly dysregulated miRNAs in OSCC patients compared to healthy controls. Based on our results, salivary miRNAs might provide a non-invasive and cost-effective method in the diagnosis of OSCC, and also to monitor more easily its evolution and therapeutic response and therefore aid in the establishment of specific therapeutic strategies.

Non invasive imaging technologies in the early diagnosis of oral squamous cell carcinoma: An overview

2007 ◽  
Vol 82 ◽  
pp. S38-S39
Author(s):  
M. Petruzzi ◽  
L. Pastore ◽  
M. De Benedittis ◽  
M. Turco ◽  
G. Gianfranco ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Early Diagnosis ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Imaging Technologies ◽  
Non Invasive

scholarly journals Overexpression of Angiogenic Factors and Matrix Metalloproteinases in the Saliva of Oral Squamous Cell Carcinoma Patients: Potential Non-Invasive Diagnostic and Therapeutic Biomarkers

2021 ◽  
Author(s):  
Meijuan Cai ◽  
Zhichao Zheng ◽  
Zhibao Bai ◽  
Kexiong Ouyang ◽  
Qiuyu Wu ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Matrix Metalloproteinases ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Expression Patterns ◽  
Bioinformatic Analysis ◽  
Angiogenic Factors ◽  
Healthy Controls ◽  
Non Invasive

Abstract Backgound: Salivary biomarkers hold huge potential for the non-invasive diagnosis of oral squamous cell carcinoma. Angiogenic factors and matrix-metalloproteinases (MMPs) are highly expressed in OSCC tissue, but their expression in the saliva is unknown. This study aimed to analyze the levels of angiogenic factors and MMPS in tumor-tissue and saliva of OSCC patients. Methods: OSCC-tissue, adjacent normal tissue (ANT), saliva from OSCC patients, and healthy controls were obtained. The expression patterns of angiogenic factors and MMPs were analyzed by immunohistochemistry, protein chip array, and RT-qPCR. Results: Results showed higher expression of ANG, ANG-2, HGF, PIGF, VEGF, MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, and TIMP-2 in OSCC-tissues compared to the ANT. Among the overexpressed markers in OSCC-tissues, HGF, VEGF, PIGF, PDGF-BB, MMP-1, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, and TIMP-2 were significantly upregulated in the saliva of OSCC patients compared to healthy controls. Conclusions: The levels of HGF, VEGF, PIGF, PDGF-BB, MMP-1, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, and TIMP-2 were upregulated both in OSCC tissue and saliva of OSCC patients. Bioinformatic analysis revealed the correlation of these factors with patient survival and cancer functional states in head and neck cancer, indicating these factors as possible saliva-based non-invasive diagnostic/prognostic markers and therapeutic targets of OSCC.

Sign in / Sign up

Export Citation Format

Share Document