Effect of hydro- alcoholic extract of Heracleum persicum during pregnancy on liver enzymes (AST-ALT-ALP) and biochemical factors(Albumin and protein) of infant male rats.

Aims: Rheumatoid arthritis (RA) is characterized by the onset of oxidative stress. This study aimed to evaluate the enhancing of extra virgin olive (EVOO) and Evening primrose oil (EPO) on oxidative stress and liver enzymes in male Wistar rats and compare between them. Place and Duration: Faculty of Science biochemistry department, Between July 2018 and August 2018. Methodology: A Subcutaneous injection of 200 µl of Freund's complete adjuvant into a footpad of the right hind leg of Wistar male rats at two consecutive days induced RA. Rats received EVOO and EPO daily by oral gavage needle with gauge 18 at doses of 5 mg/kg b.wt./day. for 10 and 21 days. No loss was recorded in the experimental rats. Results: A significant depletion in serum Reduced glutathione content (GSH), glutathione peroxidase (GPX), and glutathione s transferase activities (GST) in arthritic rats compared to normal rats after 10 and 21 days of induction which improved significantly after 10 and 21 days of EPO and EVOO treatments. EPO and EVOO treatments for 21 days increased the GSH and GPX compared to 10 days treatments while no difference in GST activity. EVOO treatment improved GSH and GPX after 10 and 21 days than EPO treatment. The elevated uric acid levels in arthritic rats were markedly ameliorated as a result of EVOO and EPO treatment administration. Increased lipid peroxidation products (MDA), rheumatoid factor, and liver enzyme (Alanine transaminase ALT and Aspartate transaminase AST) were recorded in arthritic rats and they significantly progressed after EPO and EVOO treatments for 10 and 21 days but EVOO had the best effect at 21 days. Conclusion: EVOO and EPO showed significant antioxidant efficacies and improved affected liver enzymes due to rheumatoid arthritis onset. When comparing olive oil has more antioxidant properties than evening primrose oil, so we recommend more studies on olive oil combination with anti-arthritic medications to improve their efficacies with less toxicity.

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Effect of a hydro-alcoholic extract of Melissa officinalis on passive avoidance learning and memory

Journal of Herbmed Pharmacology ◽

10.15171/jhp.2019.19 ◽

2019 ◽

Vol 8 (2) ◽

pp. 120-125 ◽

Cited By ~ 1

Author(s):

Zahra Dehbani ◽

Alireza Komaki ◽

Farshid Etaee ◽

Siamak Shahidi ◽

Masoumeh Taheri ◽

...

Keyword(s):

Passive Avoidance ◽

Learning And Memory ◽

Avoidance Learning ◽

Saline Group ◽

Passive Avoidance Learning ◽

Male Rats ◽

Control Group ◽

Alcoholic Extract ◽

Melissa Officinalis ◽

Significant Difference

Introduction: Melissa officinalis (MO) or lemon balm is traditionally used as a sedative and anti-spasm herbal medicine. There is also evidence that this plant has effects on learning and memory. This study examined the effect of a hydro-alcoholic extract of MO on passive avoidance learning (PAL) and memory in male rats. Methods: A total of 40 adult male Wistar rats were randomly distributed into four groups (200 to 220 g; n = 10 per group); three dose groups (50, 100, and 200 mg/kg of the hydro-alcoholic extract of MO) and vehicle control (saline) group. Saline or doses of extract were administered daily for 14 days by oral gavage. The rats were trained to enter the shuttle box to record their behavior in the PAL task. A retrieval test was performed 24 hours following training. Results: A significant difference was seen in performance among MO groups and the control. MO administered animals had a decreased number of acquisition trials (P < 0.05). In the retention task, MO administered animals had an increased step-through latency (SLT) (P < 0.01), and a decreased latency in the dark compartment (P < 0.001) compared to the control group. Conclusion: The results of the study show that MO can improve learning and memory in the PAL task. Further investigation is needed to enhance our understanding of the neurobiological mechanisms of the MO extract and its effects on learning and memory.

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Effects Of Vitis Vinifera Leave Hydro-Alcoholic Extract On Reproductive Parameters In Adult Normal Male Rats.

Journal of Physiology and Pharmacology Advances ◽

10.5455/jppa.20130626011300 ◽

2013 ◽

Vol 3 (6) ◽

pp. 159

Author(s):

Afzalzadeh Reza ◽

Amirzargar Ashraf ◽

Ahangarpour Akram ◽

KazemiVarnamkhasti Mohammad ◽

Ganjali Hadi ◽

...

Keyword(s):

Vitis Vinifera ◽

Male Rats ◽

Normal Male ◽

Alcoholic Extract ◽

Reproductive Parameters

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Emotional, endocrine and brain anandamide response to social challenge in infant male rats

Psychoneuroendocrinology ◽

10.1016/j.psyneuen.2013.04.004 ◽

2013 ◽

Vol 38 (10) ◽

pp. 2152-2162 ◽

Cited By ~ 13

Author(s):

Eva M. Marco ◽

Maria Luisa Scattoni ◽

Cinzia Rapino ◽

Chiara Ceci ◽

Nicole Chaves ◽

...

Keyword(s):

Male Rats ◽

Social Challenge ◽

Infant Male

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The Effect of Hydro-Alcoholic Extract of Ocimum basilicum on CaCl2-Induced Cardiac Arrhythmias in Rats

Jentashapir Journal of Cellular and Molecular Biology ◽

10.5812/jjcmb.110309 ◽

2020 ◽

Vol In Press (In Press) ◽

Author(s):

Mohammad Kheradmandpour ◽

Seyed Abolfazl Aminifar ◽

Mahin Dianat

Keyword(s):

Cardiac Arrhythmias ◽

Therapeutic Potential ◽

Heart Rhythm ◽

Ocimum Basilicum ◽

Lifestyle Changes ◽

Male Rats ◽

Control Group ◽

Alcoholic Extract ◽

Sprague Dawley ◽

Tissue Samples

: Ocimum basilicum (OB) contains more than 30 plant species that are found in different regions, especially in Africa. OB contains various substances, especially Chavikol, Methyl Ether or Strangol, Linalool, and Agenol, which have antioxidant, antihypertensive, antidiabetic, and anti-lipid properties. On the other hand, along with lifestyle changes, the prevalence of cardiovascular diseases, such as arrhythmias, which cause high mortality, is increasing. Moreover, the modern drugs used for arrhythmia can also induce another type of abnormal heart rhythm. Regarding the effectiveness and fewer side effects of herbal medicine, we decided to study the preventive effects of hydro-alcoholic extract of OB on CaCl2-induced arrhythmias in rats. Male rats (Sprague-Dawley) were divided into the control group (2 weeks, normal saline, gavage) and three groups receiving different concentrations of hydro-alcoholic extract of OB (100, 200, and 400 mg/kg, gavage for 2 weeks). The arrhythmia model was established using CaCl2 (IV, 140 mg/kg). The number of ventricular fibrillation (VF), ventricular premature (PVC), and ventricular tachycardia (VT) were studied. Also, the oxidative stress parameters, such as malondialdehyde (MDA) and superoxide dismutase (SOD), were measured in heart tissue samples. Statistical analysis was done using Fisher's exact test and ANOVA, and also, P < 0.05 was considered as a significant level. The obtained results showed that administration of OB caused a decrease in MDA and an increase in SOD levels, which were associated with improved electrocardiogram parameters and reduced cardiac arrhythmias, which suggests the therapeutic potential of the plant in cardiovascular disorders.

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Toxicity of AgNPs upon Liver Function and Positive Role of Tinospora Cordifolia: In Vivo

10.53350/pjmhs211562164 ◽

2021 ◽

Vol 15 (6) ◽

pp. 2164-2166

Author(s):

Samed Abdul Jabbar Ramadhan ◽

Ozdan Akram Ghareeb

Keyword(s):

Silver Nanoparticles ◽

Liver Function ◽

Liver Dysfunction ◽

Biochemical Parameters ◽

Liver Enzymes ◽

Male Rats ◽

Tinospora Cordifolia ◽

Control Group ◽

Positive Role

This experiment was premeditated to probe the toxic impact of Silver Nanoparticles (AgNPs) upon liver function parameters in male rats, as well as to demonstrate the protective effect of Tinospora cordifolia (T.C) against liver function disorders caused by AgNPs. Twenty-four rats were classified into four packs, six ones for every pack. Rats were included by control animals without treatment, while the AgNPs group included rats treated with 50μl/kg / day AgNPs. In the AgNPs + T.C group, rats were co-administered of AgNPs at a dose of 50μl/kg with T.C at a dose of 400 mg/kg. Whereas, animals of the T.C pack were treated with 400 mg/kg of T.C. The duration of the experiment was 28 days. After blood samples were taken at the conclusion of the experiment. The levels of biochemical parameters (liver enzymes) such as alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase were evaluated to detect liver function abnormalities. Thwe Pw A t e dewoe t ste user e dnoAiwoe se dsnd disnte dnirease in the activity of serum liver enzymes in comparison to the control group (p < 0.05).While T. cordifolia co-treatment significantly reduced the levels of activities of those enzymes. Therefore, it can be considered that T. cordifolia effectively contributes to the protection against liver dysfunction caused by AgNPs in rats. Keywords : Silver Nanoparticles, liver dysfunction, biochemical parameters.

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Protective Effect of Thyme Honey against Valproic Acid Hepatotoxicity in Wistar Rats

BioMed Research International ◽

10.1155/2021/8839898 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Rezvan Omidipour ◽

Leila Zarei ◽

Mandana Beigi Boroujeni ◽

Asghar Rajabzadeh

Keyword(s):

Valproic Acid ◽

Liver Enzymes ◽

Hepatic Necrosis ◽

Male Rats ◽

Enzyme Analysis ◽

Antioxidant Compounds ◽

Tissue Samples ◽

Biochemical Evaluation ◽

Hematoxylin Eosin ◽

Thyme Honey

Introduction. Valproic acid is a medication most commonly used in the treatment of emotional and neurological depression, psychological imbalances, epilepsy, and bipolar disorder. Dark honey, like thyme honey, contains more antioxidant compounds than other samples. The purpose of this study was to evaluate the effect of thyme honey on the potential hepatic effects of valproic acid. Methods. In this study, 48 male rats were randomly divided into 8 groups ( n = 6 ): G1 (control): healthy rats (normal saline 0.9%), G2: thyme honey (1 g/kg), G3: thyme honey (2 g/kg dose), G4: thyme honey (3 g/kg dose), G5: VPA (500 mg/kg), G6: VPA (500 mg/kg) and thyme honey (1 g/kg), G7: VPA (500 mg/kg) and thyme honey (2 g/kg dose), and G8: VPA (500 mg/kg) and thyme honey (3 g/kg dose). Groups G1 to G5 received the drug for 28 days. On day 14, administration of thyme honey for G6 to G8 groups was carried out using gavage until day 28. VPA was administered one hour after honey. To carry out the biochemical evaluation, blood samples were collected from all the groups and their serums were used for MDA, TAC, and liver enzymes (AST, ALT, and GGT). Tissue samples of each rat were also removed for histological studies with hematoxylin-eosin and Masson’s trichrome staining. Results. The use of thyme honey significantly improved the histopathological parameters of the liver tissue, including hypertrophic degeneration and nucleus alteration, expansion of sinusoids, fibrosis and hepatic necrosis, and inflammation as well as hypertrophy of Kupffer cells. In the groups receiving VPA, the rate of lipid peroxidation increased, which indicates the destruction of the liver cell membrane due to drug consumption. TAC levels also increased following increase in thyme honey dosage ( p ≤ 0.05 ). The results of liver enzyme analysis showed a decrease in AST and ALT levels in the G6 group and a decrease in GGT level in the G8 group ( p ≤ 0.05 ). Conclusion. Based on the results of this study, it seems that high percentage of antioxidants in thyme honey enabled it to improve hepatic complications and reduce the rate of hepatocellular destruction.

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