Efek Antibakteri Ekstrak Aquades Buah Kurma (Phoenix dactylifera L.) Varietas Ajwa terhadap Staphylococcus aureus Secara In Vitro

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Lu'lu Ulul Albab ◽  
Usep Abdullah Husin ◽  
Buti Azfiani Azhali ◽  
Titik Respati ◽  
Ratna Dewi Indi Astuti
Keyword(s):  
Staphylococcus Aureus ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Antibacterial Properties ◽  
Phoenix Dactylifera ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Phoenix Dactylifera L

Penemuan antimikrob menjadi hal yang sangat penting dalam pengobatan dan kemajuan medis. Tantangan dalam pengobatan menggunakan antibiotik adalah resistensi bakteri terhadap antibiotik yang digunakan. Salah satu bakteri yang mengalami resistensi adalah Staphylococcus aureus. Oleh karena itu, diteliti alternatif pengobatan dengan menggunakan tumbuhan yang berpotensi memiliki kemampuan antibakteri. Buah kurma varietas Ajwa telah diteliti memiliki beberapa senyawa aktif yang berpotensi sebagai antibakteri dan antioksidan. Kurma Ajwa juga telah disebutkan di dalam Al-Quran dan Al-Hadits. Tujuan penelitian ini adalah mengetahui efek antibakteri ekstrak akuades buah kurma Ajwa dengan melihat luas zona hambat serta konsentrasi hambat minimum (KHM) dan konsentrasi bunuh minimum (KBM) pada Staphylococcus aureus. Penelitian ini menggunakan metode ekperimental secara in vitro dan bersifat deskriptif observasional dengan pendekatan kuantitatif. Metode yang digunakan adalah metode difusi dengan menggunakan sumuran dan metode dilusi padat. Penelitian ini dilakukan di Laboratorium Terpadu Mikrobiologi Poltekkes Bandung. Didapatkan rerata hasil zona hambat adalah 5,87 mm yang termasuk kategori sedang, KHM pada konsentrasi 50%, dan KBM belum dapat ditemukan. Berdasar atas uji statistik, hasil penelitian uji difusi menunjukkan bahwa ekstrak akuades buah kurma (Phoenix dactylifera L.) varietas Ajwa berpengaruh menghambat pertumbuhan bakteri Staphylococcus aureus. ANTIBACTERIAL EFFECT OF EXTRACT AQUADES AJWA DATE (PHOENIX DACTYLIFERA L.) AGAINST STAPHYLOCOCCUS AUREUS IN VITROThe discovery of antimicrobials has been significantly important for current treatments and the future developments in the medical field. One of the challenges faced in the usage of antibiotics is the resistance imposed by the bacteria towards the antibiotics itself. An example of such bacteria that experiences thisresistance is Staphylococcus aureus. As a result, studies regarding alternatives of treatments, specifically those utilizing various plants that may potentially have antibacterial properties are conducted. Previous studies have already shown that date palms of the Ajwa variety, known as Phoenix dactylifera, contains several active compounds that are potential antioxidants and exhibit antibacterial properties. The Ajwa date palm has also been mentioned in several scriptures of the Al-Quran and Al-Hadits. The objective of this study was to understand the antibacterial properties of aquadest-extracted Phoenix dactylifera through the measurement of the area of the zone of inhibition, the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC) of Staphylococcus aureus. This study involves the use of an experimental method, known as in vitro, and is also a descriptive observational study with a quantitative approach. The methodology used consists of the well diffusion method and the solid dilution method. This study was conducted at Laboratorium Terpadu Mikrobiologi Poltekkes Bandung. The results of this study show an average zone of inhibition of 5.87 mm which is categorized as medium, a minimum inhibitory concentration of 50%, and a minimum bactericidal concentration that is yet to be found. Based on statistical tests, the result of the diffusion method shows that the aquadest-extracted Phoenix dactylifera is influential in inhibiting the growth of the Staphylococcus aureus bacteria.

scholarly journals EFEKTIVITAS EKSTRAK ETANOL BIJI KURMA MESIR (Phoenix dactylifera L.) SEBAGAI ANTIBAKTERI TERHADAP Streptococcus pyogenes SECARA IN VITRO

2019 ◽  
Vol 19 (3) ◽  
Author(s):  
Lilia Putri Pratama
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Streptococcus Pyogenes ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Agar Plate ◽  
Ethanol Extract ◽  
Phoenix Dactylifera ◽  
Blood Agar Plate ◽  
Phoenix Dactylifera L

Abstrak. Kurma (Phoenix dactylifera L.) telah dikonsumsi secara luas di seluruh duniadan diketahui memiliki banyak manfaat di bidan kesehatan. Salah satu mafaatnya yaitu sebagai obat terhadap faringitis yang disebabkan oleh bakteri Streptococcus pyogenes. Terdapat ±517.000 kematian pertahun yang disebabkan oleh infeksi Streptococcus pyogenes dan komplikasinya. Sejauh ini telah banyak studi mengenai manfaat daging kurma, namun manfaat terhadap biji kurma belum banyak diteliti. Maka dari itu penelitian ini bertujuan untuk menguji efek antibakteri ekstrak etanol biji kurma Mesir (Phoenix dactylifera L.) pada Streptococus pyogenes  secara in vitro dengan uji Konsentrasi Hambat Minimal (KHM) menggunakan metode dilusi serial dan uji Konsentrasi Bunuh Minimal (KBM) dengan inokulasi pada media agar darah. Konsentrasi yang digunakan dalam penelitian ini adalah 1428.57 mg/ml, 714,29 mg/ml, 357.14 mg/ml, 178.57 mg/ml, 89.29 mg/ml, 44,64 mg/ml, 22,32 mg/ml, dan 11,16 mg/ml. Dari penelitian tersebut didapatkan bahwa Konsentrasi Hambat Minimal (KHM) tidak dapat ditentukan dan nilai Konsentrasi Bunuh Minimal (KBM) sebesar 1428.57 mg/ml. Ekstrak etanol biji kurma Mesir (Phoenix dactylifera L.) efektif sebagai antibakteri terhadap Streptococcus pyogenes dengan nilai signifikan 0,002 (p0,05) Kata Kunci: Biji Kurma Mesir, Phoenix dactylifera L., Streptococcus pyogenes, faringitis Abstract. Dates (Phoenix dactylifera L.) are widely consumed in various parts of the world and are known to have many benefits in the health sector. One of them is pharyngitis medication caused by Streptococcus pyogenes. There are ±517.000 deaths per year which caused by Streptococcus pyogenes infection and its complication. So far there have been many studies on the benefits of date flesh, but the benefits of the seeds have not been widely studied. The aim of this study is to test the anti-bacterial effect of the ethanol extract of Egyptian Phoenix dactylifera L. seed on Streptococcus pyogenes in vitro by Minimum Inhibitory Concentration test using serial dilution method and Minimum Bactericidal Concentration (MBC) in blood agar plate inoculates.  The concentration used in this study are 1428.57 mg/ml, 714,29 mg/ml, 357.14 mg/ml, 178.57 mg/ml, 89.29 mg/ml, 44,64 mg/ml, 22,32 mg/ml, and 11,16 mg/ml. From this study the Minimum Inhibitory Concentration (MIC) can not be determined and the Minimum Bactericidal Concentration is 1428.57 mg/ml. Ethanol extract of Egyptian Phoenix dactylifera L. seed effective as antibacteri against Streptococcus pyogenes with a significant value of 0,002 (p 0,05).    Keywords: date palm seeds, Phoenix dactylifera L, Streptococcus pyogenes, pharyngitis. 

scholarly journals Efektivitas ekstrak etanol biji kurma mesir (Phoenix dactylifera L.) sebagai antibakteri terhadap Streptococcus pyogenes secara in vitro

2019 ◽  
Vol 19 (3) ◽  
Author(s):  
Lilia Putri Pratama ◽  
Marijam Purwanta ◽  
Ema Qurnianingsih
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Streptococcus Pyogenes ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Agar Plate ◽  
Ethanol Extract ◽  
Phoenix Dactylifera ◽  
Blood Agar Plate ◽  
Phoenix Dactylifera L

Abstrak. Kurma (Phoenix dactylifera L.) telah dikonsumsi secara luas di seluruh duniadan diketahui memiliki banyak manfaat di bidan kesehatan. Salah satu mafaatnya yaitu sebagai obat terhadap faringitis yang disebabkan oleh bakteri Streptococcus pyogenes. Terdapat ±517.000 kematian pertahun yang disebabkan oleh infeksi Streptococcus pyogenes dan komplikasinya. Sejauh ini telah banyak studi mengenai manfaat daging kurma, namun manfaat terhadap biji kurma belum banyak diteliti. Maka dari itu penelitian ini bertujuan untuk menguji efek antibakteri ekstrak etanol biji kurma Mesir (Phoenix dactylifera L.) pada Streptococus pyogenes  secara in vitro dengan uji Konsentrasi Hambat Minimal (KHM) menggunakan metode dilusi serial dan uji Konsentrasi Bunuh Minimal (KBM) dengan inokulasi pada media agar darah. Konsentrasi yang digunakan dalam penelitian ini adalah 1428.57 mg/ml, 714,29 mg/ml, 357.14 mg/ml, 178.57 mg/ml, 89.29 mg/ml, 44,64 mg/ml, 22,32 mg/ml, dan 11,16 mg/ml. Dari penelitian tersebut didapatkan bahwa Konsentrasi Hambat Minimal (KHM) tidak dapat ditentukan dan nilai Konsentrasi Bunuh Minimal (KBM) sebesar 1428.57 mg/ml. Ekstrak etanol biji kurma Mesir (Phoenix dactylifera L.) efektif sebagai antibakteri terhadap Streptococcus pyogenes dengan nilai signifikan 0,002 (p0,05)Kata Kunci: Biji Kurma Mesir, Phoenix dactylifera L., Streptococcus pyogenes, faringitis Abstract. Dates (Phoenix dactylifera L.) are widely consumed in various parts of the world and are known to have many benefits in the health sector. One of them is pharyngitis medication caused by Streptococcus pyogenes. There are ±517.000 deaths per year which caused by Streptococcus pyogenes infection and its complication. So far there have been many studies on the benefits of date flesh, but the benefits of the seeds have not been widely studied. The aim of this study is to test the anti-bacterial effect of the ethanol extract of Egyptian Phoenix dactylifera L. seed on Streptococcus pyogenes in vitro by Minimum Inhibitory Concentration test using serial dilution method and Minimum Bactericidal Concentration (MBC) in blood agar plate inoculates.  The concentration used in this study are 1428.57 mg/ml, 714,29 mg/ml, 357.14 mg/ml, 178.57 mg/ml, 89.29 mg/ml, 44,64 mg/ml, 22,32 mg/ml, and 11,16 mg/ml. From this study the Minimum Inhibitory Concentration (MIC) can not be determined and the Minimum Bactericidal Concentration is 1428.57 mg/ml. Ethanol extract of Egyptian Phoenix dactylifera L. seed effective as antibacteri against Streptococcus pyogenes with a significant value of 0,002 (p 0,05).Keywords: date palm seeds, Phoenix dactylifera L, Streptococcus pyogenes, pharyngitis.

scholarly journals Antibacterial Properties of Local Malaysian Trigona Sp. Honey Towards Different of Pathogenic Bacteria in Vitro

2021 ◽  
Vol 13 (1) ◽  
pp. 1-9
Author(s):  
Fawzia Jassim Shalsh ◽  
Ahmed Mohammed Khalaf ◽  
Mohammed Hafiz ◽  
Imad Ibrahim Ali
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Therapeutic Effects ◽  
Disc Diffusion ◽  
Diffusion Test

Honey is one of the oldest natural medicines known with a very high therapeutic value. Nowadays, in the medical field, several important therapeutic effects of honey have been elucidated. This study was conducted to reveal the antimicrobial activity of the commercially available local Malaysian Trigona sp. honey towards different pathogenic bacteria specifically Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, and Salmonella typhimurium. The Minimum inhibitory concentration and Minimum bactericidal concentration were determined by the disc-diffusion test and agar well diffusion test. Different concentration of the honey was tested in the disc-diffusion and agar well diffusion test. The results of these tests were in terms of Inhibition zone diameter. The results obtained from the current study are the dilution of different concentration of honey from Trigona sp. are very significant because the only net concentration of both of honey Trigon a sp. possessed antimicrobial properties in term of Minimum inhibitory concentration) and Minimum bactericidal concentration. The result also can say that Trigona sp. honey possessed antibacterial properties and can be used as alternative medicine in the veterinary field in the future.

scholarly journals Anti-Shigellosis Activity of Cola anomala Water/Ethanol Pods Extract on Shigella flexneri-Induced Diarrhea in Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Henri Wambe ◽  
Paul Aimé Noubissi ◽  
Michel Archange Fokam Tagne ◽  
Angèle Foyet Fondjo ◽  
Gaëtan Olivier Fankem ◽  
...  
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Shigella Flexneri ◽  
White Blood Cells ◽  
Diffusion Method ◽  
Albino Rats ◽  
Disk Diffusion Method

This study was undertaken to evaluate the activities of water/ethanol Cola anomala pods extract. In vitro antimicrobial susceptibility was determined by the disk diffusion method; the minimum inhibitory concentration and minimum bactericidal concentration were determined by agar dilution technique. In vivo, shigellosis was induced in healthy Wistar albino rats by oral administration of Shigella flexneri inoculum, 12 × 108 CFU/mL. At the onset of diarrhea, infected and normal control animals were subdivided into various groups treated with distilled water, with water/ethanol Cola anomala pods extract at 25, 50, or 100 mg/kg, or with ciprofloxacin, 2.5 mg/kg. After one-week treatment, rats were sacrificed, and blood and colon were collected. Blood was used for blood cell count. A portion of the colon served for histological studies while homogenate from the remaining part was centrifuged and the supernatant was collected for the determination of NO, PGE2, IL-1β, and TNF-α levels. In vitro, water/ethanol Cola anomala pods extract showed to be bactericidal, with a minimum inhibitory concentration of 2.0 mg/mL and a minimum bactericidal concentration of 3.0 mg/mL. In diarrheic rats, the extract significantly (P<0.01) increased the white blood cells and significantly (P<0.01) decreased stool Shigella density from the first to the seventh day of treatment. It partially restored the structure of eroded intestine epithelium and prevented weight loss; the dose dependently and significantly (P<0.001) decreased NO, IL-1β, and TNF-α production in the colon and was found to have no significant effect on PGE2 production. These results support the use of this plant in traditional medicine in the treatment of gastrointestinal ailments.

scholarly journals Antibacterial Activity and Cytotoxic Effect of Pelargonium peltatum (Geranium) against Streptococcus mutans and Streptococcus sanguinis

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Samantha Coronado-López ◽  
Stefany Caballero-García ◽  
Miguel Angel Aguilar-Luis ◽  
Fernando Mazulis ◽  
Juana del Valle-Mendoza
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Streptococcus Mutans ◽  
Inhibitory Concentration ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Root Extract ◽  
Streptococcus Sanguinis ◽  
Pelargonium Peltatum ◽  
High Concentrations

Objective. To evaluate the in vitro antibacterial and cytotoxic properties of the methanolic extract of Pelargonium peltatum (geranium) against Streptococcus mutans (ATCC 25175) and Streptococcus sanguinis (ATCC 10556). Methods. Three extracts of P. peltatum were prepared using the leaf, stem, and root. Nine independent assays were prepared for each type of extract with chlorhexidine at 0.12% as the positive control. The agar diffusion method was performed to determine the antibacterial properties of each extract. The minimum inhibitory concentration (MIC) was determined using the microdilution method, and the cytotoxicity was analyzed by means of the MTT reduction test using a MDCK cell line. Results. The root extract had the highest antibacterial effect with a mean result of (27.68 ± 0.97) mm and (30.80 ± 0.55) mm against S. mutans and S. sanguinis, respectively. The minimum inhibitory concentration for the leaf and root extracts was 250 mg/mL for S. mutans and 125 mg/mL for S. sanguinis. Cytotoxicity assays showed that both extracts had a low cytotoxicity at high concentrations. The cellular viability was highest for the root extract at 95.3% followed by the stem extract at 80.8% and finally the leaf extract with 75.4%. Conclusions. These findings show the antibacterial properties of the methanolic extracts of P. pelargonium against S. mutans and S. sanguinis. These extracts were not cytotoxic at high concentrations.

scholarly journals In vitro antibacterial activity of shrimp chitosan against Salmonela paratyphi and Staphylococcus aureus

1970 ◽  
Vol 24 (2) ◽  
pp. 185-190 ◽  
Author(s):  
Md Monarul Islam ◽  
Shah Md Masum ◽  
Khandaker Rayhan Mahbub
Keyword(s):  
Staphylococcus Aureus ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Antimicrobial Properties ◽  
Chitosan Solution ◽  
Antimicrobial Activities ◽  
Processing Waste ◽  
Bacterial Strains

Antimicrobial properties of chitosan extracted from indigenous shrimp processing waste were determined against one gram-negative (Salmonella Paratyphi) and one gram-positive bacterium (Staphylococcus aureus) in vitro. The antimicrobial activities of chitosan were explored by calculation of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) in media supplemented with 128, 138, 168, 192, 240, 288, 300 and 320 ppm chitosan solution adjusted to pH 6 or 7. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the prepared chitosan was 288 and 300 ppm for both bacterial strains.  These results indicate that chitosan from indigenous shrimp processing waste could be used as an effective antibacterial agent in the food industry. DOI: http://dx.doi.org/10.3329/jbcs.v24i2.9707 Journal of Bangladesh Chemical Society, Vol. 24(2), 185-190, 2011

scholarly journals Antibacterial Property of Extract of Erveniastrum nepalense (Edible Lichen) Collected from Hilly Regions of Eastern Nepal

2019 ◽  
Vol 6 ◽  
pp. 51-58
Author(s):  
Saraswati Yonghang ◽  
Suman Rai ◽  
Shiv Nandan Sah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Antibacterial Property ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Klebsiella Spp

Objectives: To evaluate the antibacterial property of Everniastrum nepalense(edible lichen)collected from eastern part of Nepal and determine Minimum Inhibitory Concentration (MIC) of lichen extract. Methods: Everniastrum nepalense was collected from local Rai and Limbu community in a plastic bag from four hilly districts (Pachthar, Taplejung, Dhankuta and Bhojpur) of Eastern, NepaMoisture content in lichen samples were removed by air drying and grinded to powdery form. The methanolic extracts of lichens of different places were prepared by solvent extraction process using Soxhlet apparatus and tested against human pathogenic bacteria by disc diffusion method. Then, Minimum Inhibitory Concentration (MIC) of lichen extracts was determined using well diffusion method. Results: The lichens extract against the test bacterial isolates were performed by agar well diffusion method. Overall, it was observed that all these lichen extracts from Eastern Nepal had inhibitory effect on both gram-positive and gram-negative bacteria. Out of seven bacterial samples used, all bacterial samples were inhibited by the lichen extracts except Escherichia coli. The effect of lichen extract collected from Bhojpur district had maximum zone of inhibition against Staphylococcus aureus, Salmonella Typhi and Klebsiella spp were found to be 13 mm, 14 mm and 14 mm respectively. Similarly, zone of inhibition against Bacillus subtilis was found to be 17 mm from samples of Dhankuta, which was highest value than other 3 districts viz; Bhojpur, Taplejung and Pachthar District. Likewise, Pseudomonas aeruginosa showed highest inhibition value i.e. 18 mm on Pachthar sample whereas, Shigella showed 15 mm on Dhankuta sample. This study revealed that inhibitory capacity of edible lichen (Everniastrum nepalense) against test bacteria was not the same. It varied from place to place. Conclusion: This study has concluded that Everniastrum nepalensehas potential antibacterial property against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Salmonella spp, Shigella spp, Klebsiella spp and Pseudomonas aeruginosa except E. coli along with nutritional value and can be used as safe alternative and economic herbal medicine to treat infectious diseases.

scholarly journals THE EFFECT OF PROPOLIS EXTRACT AND PROPOLIS CANDIES ON THE GROWTH OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS ATCC 43718

2017 ◽  
Vol 10 (17) ◽  
pp. 26
Author(s):  
Khodijah Khodijah ◽  
Ratna Farida ◽  
Nurtami Soedarsono
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Spectrophotometric Analysis ◽  
Propolis Extract ◽  
Post Exposure ◽  
Colony Forming Units

Objective: This experiment aimed to analyze the effect of propolis extract and propolis containing candies on the growth of Aggregatibacter actinomycetemcomitans using spectrophotometric analysis and colony-forming units (CFU) counts.Methods: After A. actinomycetemcomitans were exposed to propolis extract and candies, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined with spectrophotometry and post-exposure colony counting.Results: The MIC of propolis extract against A. actinomycetemcomitans was determined to be 10%, and the MBC was 20%. A decrease in the total CFU count of A. actinomycetemcomitans was observed after propolis extract and candy exposure.Conclusions: Propolis extract and propolis candies were effective in inhibiting the growth of A. actinomycetemcomitans ATCC 43718 in vitro.

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