scholarly journals In vitro antibacterial activity of shrimp chitosan against Salmonela paratyphi and Staphylococcus aureus

1970 ◽  
Vol 24 (2) ◽  
pp. 185-190 ◽  
Author(s):  
Md Monarul Islam ◽  
Shah Md Masum ◽  
Khandaker Rayhan Mahbub
Keyword(s):  
Staphylococcus Aureus ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Antimicrobial Properties ◽  
Chitosan Solution ◽  
Antimicrobial Activities ◽  
Processing Waste ◽  
Bacterial Strains

Antimicrobial properties of chitosan extracted from indigenous shrimp processing waste were determined against one gram-negative (Salmonella Paratyphi) and one gram-positive bacterium (Staphylococcus aureus) in vitro. The antimicrobial activities of chitosan were explored by calculation of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) in media supplemented with 128, 138, 168, 192, 240, 288, 300 and 320 ppm chitosan solution adjusted to pH 6 or 7. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the prepared chitosan was 288 and 300 ppm for both bacterial strains.  These results indicate that chitosan from indigenous shrimp processing waste could be used as an effective antibacterial agent in the food industry. DOI: http://dx.doi.org/10.3329/jbcs.v24i2.9707 Journal of Bangladesh Chemical Society, Vol. 24(2), 185-190, 2011

scholarly journals Comparative Study of Phenolic Compound Antioxidant and Antimicrobial Activities of Fruits Peel and Cladodes from Tunisian Opuntia Stricta

2021 ◽  
Vol 7 (5) ◽  
pp. 1-7
Author(s):  
Lazhar Zourgui ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Minimum Inhibitory Concentration ◽  
Comparative Study ◽  
Fusarium Oxysporum ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Antimicrobial Activities ◽  
Bacterial Strains ◽  
Inhibition Zones ◽  
Antioxidant And Antimicrobial Activities

Similarly, the bacterial strains tested were most sensitive to fruit peels than especially against Staphylococcus aureus with inhibition zones (DIZ), minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 15.22±2.14 mm, 4.68 mg/mL and 37.5 mg/mL respectively. However, aqueous extract of cladodes have high antifongical activity particularly against Fusarium oxysporum with DIZ (20±0.2 mm), MIC (4.68mg/mL) and MFC (75 mg/mL) value

scholarly journals Antibacterial Properties of Local Malaysian Trigona Sp. Honey Towards Different of Pathogenic Bacteria in Vitro

2021 ◽  
Vol 13 (1) ◽  
pp. 1-9
Author(s):  
Fawzia Jassim Shalsh ◽  
Ahmed Mohammed Khalaf ◽  
Mohammed Hafiz ◽  
Imad Ibrahim Ali
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Therapeutic Effects ◽  
Disc Diffusion ◽  
Diffusion Test

Honey is one of the oldest natural medicines known with a very high therapeutic value. Nowadays, in the medical field, several important therapeutic effects of honey have been elucidated. This study was conducted to reveal the antimicrobial activity of the commercially available local Malaysian Trigona sp. honey towards different pathogenic bacteria specifically Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, and Salmonella typhimurium. The Minimum inhibitory concentration and Minimum bactericidal concentration were determined by the disc-diffusion test and agar well diffusion test. Different concentration of the honey was tested in the disc-diffusion and agar well diffusion test. The results of these tests were in terms of Inhibition zone diameter. The results obtained from the current study are the dilution of different concentration of honey from Trigona sp. are very significant because the only net concentration of both of honey Trigon a sp. possessed antimicrobial properties in term of Minimum inhibitory concentration) and Minimum bactericidal concentration. The result also can say that Trigona sp. honey possessed antibacterial properties and can be used as alternative medicine in the veterinary field in the future.

Efek Antibakteri Ekstrak Aquades Buah Kurma (Phoenix dactylifera L.) Varietas Ajwa terhadap Staphylococcus aureus Secara In Vitro

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Lu'lu Ulul Albab ◽  
Usep Abdullah Husin ◽  
Buti Azfiani Azhali ◽  
Titik Respati ◽  
Ratna Dewi Indi Astuti
Keyword(s):  
Staphylococcus Aureus ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Antibacterial Properties ◽  
Phoenix Dactylifera ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Phoenix Dactylifera L

Penemuan antimikrob menjadi hal yang sangat penting dalam pengobatan dan kemajuan medis. Tantangan dalam pengobatan menggunakan antibiotik adalah resistensi bakteri terhadap antibiotik yang digunakan. Salah satu bakteri yang mengalami resistensi adalah Staphylococcus aureus. Oleh karena itu, diteliti alternatif pengobatan dengan menggunakan tumbuhan yang berpotensi memiliki kemampuan antibakteri. Buah kurma varietas Ajwa telah diteliti memiliki beberapa senyawa aktif yang berpotensi sebagai antibakteri dan antioksidan. Kurma Ajwa juga telah disebutkan di dalam Al-Quran dan Al-Hadits. Tujuan penelitian ini adalah mengetahui efek antibakteri ekstrak akuades buah kurma Ajwa dengan melihat luas zona hambat serta konsentrasi hambat minimum (KHM) dan konsentrasi bunuh minimum (KBM) pada Staphylococcus aureus. Penelitian ini menggunakan metode ekperimental secara in vitro dan bersifat deskriptif observasional dengan pendekatan kuantitatif. Metode yang digunakan adalah metode difusi dengan menggunakan sumuran dan metode dilusi padat. Penelitian ini dilakukan di Laboratorium Terpadu Mikrobiologi Poltekkes Bandung. Didapatkan rerata hasil zona hambat adalah 5,87 mm yang termasuk kategori sedang, KHM pada konsentrasi 50%, dan KBM belum dapat ditemukan. Berdasar atas uji statistik, hasil penelitian uji difusi menunjukkan bahwa ekstrak akuades buah kurma (Phoenix dactylifera L.) varietas Ajwa berpengaruh menghambat pertumbuhan bakteri Staphylococcus aureus. ANTIBACTERIAL EFFECT OF EXTRACT AQUADES AJWA DATE (PHOENIX DACTYLIFERA L.) AGAINST STAPHYLOCOCCUS AUREUS IN VITROThe discovery of antimicrobials has been significantly important for current treatments and the future developments in the medical field. One of the challenges faced in the usage of antibiotics is the resistance imposed by the bacteria towards the antibiotics itself. An example of such bacteria that experiences thisresistance is Staphylococcus aureus. As a result, studies regarding alternatives of treatments, specifically those utilizing various plants that may potentially have antibacterial properties are conducted. Previous studies have already shown that date palms of the Ajwa variety, known as Phoenix dactylifera, contains several active compounds that are potential antioxidants and exhibit antibacterial properties. The Ajwa date palm has also been mentioned in several scriptures of the Al-Quran and Al-Hadits. The objective of this study was to understand the antibacterial properties of aquadest-extracted Phoenix dactylifera through the measurement of the area of the zone of inhibition, the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC) of Staphylococcus aureus. This study involves the use of an experimental method, known as in vitro, and is also a descriptive observational study with a quantitative approach. The methodology used consists of the well diffusion method and the solid dilution method. This study was conducted at Laboratorium Terpadu Mikrobiologi Poltekkes Bandung. The results of this study show an average zone of inhibition of 5.87 mm which is categorized as medium, a minimum inhibitory concentration of 50%, and a minimum bactericidal concentration that is yet to be found. Based on statistical tests, the result of the diffusion method shows that the aquadest-extracted Phoenix dactylifera is influential in inhibiting the growth of the Staphylococcus aureus bacteria.

scholarly journals Antimicrobial Activity of the Dichloromethane Extract from in vitro Cultured Roots of Morinda royoc and Its Main Constituents

2010 ◽  
Vol 5 (5) ◽  
pp. 1934578X1000500
Author(s):  
Janetsy Borroto ◽  
Ricardo Salazar ◽  
Alejandro Pérez ◽  
Yemeys Quiros ◽  
Martha Hernandez ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Bacterial Strains ◽  
Yeast Strains ◽  
Mic Value

The dichloromethane extract and seven anthraquinones isolated from in vitro cultured roots of Morinda royoc L. were tested for their antimicrobial activity against seven yeast and seven bacterial strains. The extract showed a minimum inhibitory concentration (MIC) value of 15.6 μg/m against all species of Candida tested; except C. glabrata (MIC 1.95 μg/mL), and it inhibited the growth of oxacillin-resistant Staphylococcus aureus (MIC 31.2 μg/mL). Only morindone showed activity against all yeast strains (MIC 1.9 μg/mL), and against oxacillin-resistant Staphylococcus aureus (MIC 15 μg/mL).

scholarly journals Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of mangosteen (Garcinia mangostana Linn.) rind extract against Aggregatibacter actinomycetemcomitans

2020 ◽  
Vol 32 (2) ◽  
Author(s):  
Ina Hendiani ◽  
Agus Susanto ◽  
Dyah Nindita Carolina ◽  
Rahmat Ibrahim ◽  
Felisha Febriane Balatif
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Periodontal Diseases ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Aggressive Periodontitis ◽  
Garcinia Mangostana

Introduction: The main contents of mangosteen (Garcinia mangostana Linn.) is xanthone with antibacterial activity. Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) was a gram-negative, facultative anaerobic bacillus that causes periodontal diseases such as localised aggressive periodontitis (LAP). Mangosteen rind extract could inhibit the growth of Porphyromonas gingivalis and A. actinomycetemcomitans. This study was aimed to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of mangosteen rind extract against A. actinomycetemcomitans. Methods: This study was an experimental and in-vitro study of antimicrobial properties of mangosteen (Garcinia mangostana Linn.).The extract of mangosteen was made by the maceration method using 70% ethanol solvent. A. actinomycetemcomitans (ATCC 43718) obtained from Laboratorium of Microbiology Airlangga University Surabaya. This study used a microdilution method and analysed using the Enzyme Linked Immune Sorbent Assay (ELISA). The determination of the MIC was measured using the formula for cell inhibition percentage. Three concentrations higher than the MIC standard were cultured on the Mueller Hinton agar. The lowest concentration on agar which was not found any growth of the bacterial colonies was determined as the MBC. Results: The results of this study indicated that the MIC and MBC of mangosteen rind extract against A. actinomycetemcomitans were 195.3 ppm and 1562.5 ppm, respectively. Conclusion: Mangosteen rind extract can inhibit and bactericide the growth of A. actinomycetemcomitans.

scholarly journals THE EFFECT OF PROPOLIS EXTRACT AND PROPOLIS CANDIES ON THE GROWTH OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS ATCC 43718

2017 ◽  
Vol 10 (17) ◽  
pp. 26
Author(s):  
Khodijah Khodijah ◽  
Ratna Farida ◽  
Nurtami Soedarsono
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Spectrophotometric Analysis ◽  
Propolis Extract ◽  
Post Exposure ◽  
Colony Forming Units

Objective: This experiment aimed to analyze the effect of propolis extract and propolis containing candies on the growth of Aggregatibacter actinomycetemcomitans using spectrophotometric analysis and colony-forming units (CFU) counts.Methods: After A. actinomycetemcomitans were exposed to propolis extract and candies, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined with spectrophotometry and post-exposure colony counting.Results: The MIC of propolis extract against A. actinomycetemcomitans was determined to be 10%, and the MBC was 20%. A decrease in the total CFU count of A. actinomycetemcomitans was observed after propolis extract and candy exposure.Conclusions: Propolis extract and propolis candies were effective in inhibiting the growth of A. actinomycetemcomitans ATCC 43718 in vitro.

In-Vitro Studies on the Sensitivity of Staphylococcus aureus to Some Ethno-Medicinal Preparations Sold around Kano, Nigeria

2011 ◽  
Vol 4 (1) ◽  
pp. 22-25 ◽  
Author(s):  
M Bashir ◽  
I Yusuf ◽  
AS Kutama
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Test Organism ◽  
Ethanolic Extract ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Herbal Preparations

Five traditional herbal preparations were sampled between May-June, 2009 in Kano. The samples were investigated for invitro antibacterial activities against clinical isolates of Staphylococcus aureus. Likewise, phytochemical screening tests were conducted to determine some of the phytochemicals present in the ethanolic and water extracts of the samples. Various concentrations of the extracts were prepared using serial doubling dilutions (5000=l/ml, 2500=g/ml, 1250=g/ml, 625=g/ml and 312.5=g/ml). All the test extracts showed slight antibacterial activity against the test organism, with ethanolic extract of sample E having the highest zone diameter of inhibition, while sample H had the lowest diameter of inhibition. The standard antibiotic disc (Gentamicin) had demonstrated the highest activity on the test organisms. The results of the Phytochemical screening revealed the presence of steroid in all the samples, tannin in samples A, C, D and E, reducing sugars in sample A, D and E respectively. The result of the minimum inhibitory concentration (MIC) was found to be above 312.5=g/ml for samples C, D and E. Keywords: Staphylococcus aureus, Herbal preparations, antibacterial activity, Phytochemical screening and minimum inhibitory concentration.

scholarly journals The PHYTOCHEMICALS, ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF Senna alata AND Senna tora LEAF EXTRACTS AGAINST BACTERIAL STRAINS CAUSING SKIN INFECTIONS

2019 ◽  
Vol 2 (1) ◽  
pp. 19
Author(s):  
Murni Halim
Keyword(s):  
Leaf Extract ◽  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Natural Drugs ◽  
Senna Alata

A study was carried out to screen for phytochemical constituents and assess the antioxidant and antimicrobial activities of Senna alata and Senna tora leaf extracts. The leaves were first dried at room temperature and 50°C in an oven prior to solvent extraction using ethanol and methanol. The in-vitro qualitative assays showed that both S. alata and S. tora leaf extracts contained bioactive and secondary metabolites components such as tannins, steroids, saponin, terpenoids, glycosides, flavonoids and phenols. The antioxidant activity and capacity test were carried out by conducting free radical of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and Ferric reduction antioxidant plasma (FRAP) assays. Both assays showed S. tora leaf extract has higher antioxidant capacity than S. alata leaf extract. The efficacy of these leaf extracts were tested against skin pathogens through agar well diffusion method. S. alata extract showed an inhibition zone (1.15 – 1.59 mm) against Pseudomonas aeruginosa while S. tora extracts exhibited a strong antimicrobial activity against S. epidermidis (inhibition zone of 12 – 16.94 mm) followed by P. aeruginosa (inhibition zone of 1 – 1.59 mm). Nonetheless, no inhibition zone was observed for S. aureus by both leaf extracts. The phytochemicals and antioxidant constituents as well as inhibitory potential on skin pathogens possessed by S. alata and S. tora leave highlighted their potential utilization in the development of natural drugs or cosmetics to treat skin related diseases or infections.

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