scholarly journals Investigation of Leishmania cutanenous Antigens in blood Specimens

2021 ◽  
Vol 26 (2) ◽  
pp. 1-8
Author(s):  
Aliaa Alnaimy ◽  
Ali B. M. Al-Waaly
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Teaching Hospital ◽  
Leishmania Major ◽  
Skin Lesions ◽  
Immunoglobulin M ◽  
Leishmania Parasite ◽  
Major Type ◽  
Serum Samples ◽  
Serological Tests ◽  
Blood Specimens

The study was conducted on 444 patients suffering from skin lesions of different ages and genders that were diagnosed by dermatologists as cutaneous leishmaniasis for people reviewing and throughout the days of the week for the department of epidemiology and transitional diseases who attended to hospitals in the city of Baghdad, namely (Al-Shaheed Dari Al Fayyad Teaching Hospital , Al-Shaheed Al Sadr Hospital , Al-Numan Teaching Hospital, Al-Karama Teaching Hospital) for the study period from October 1, 2019 until the end of March 2020. The methods of molecular diagnostics showed that multiplex pcr examined40 random samples of the parasite  that were collected throughthis technique from blood samples taken from the ulcers of infectedpeople to reveal the DNA parts of the Leishmania parasite. Specialprefixes for the Leishmania parasite species showed that 28 positive samplescontain the parasite's DNA and 12 negative samples, of which 11 are forthe Leishmania tropica type (27.5%) and 17 samples for the Leishmania major type(42.5%) at the molecular weight 1150 base pair. 30 serum samples were taken from patients diagnosed with cutaneous leishmaniasis for serological tests. The current study showed a significant decrease in immunoglobulin G for patients with cutaneous leishmaniasis by 1205.100 ± 210.155 , The current results also showed a significant decrease in the level of immunoglobulin M in patients with cutaneous leishmaniasis by 90.366 ± 26.181

scholarly journals Expression of Inducible Nitric Oxide Synthase in Skin Lesions of Patients with American Cutaneous Leishmaniasis

2002 ◽  
Vol 70 (8) ◽  
pp. 4638-4642 ◽  
Author(s):  
Muna Qadoumi ◽  
Inge Becker ◽  
Norbert Donhauser ◽  
Martin Röllinghoff ◽  
Christian Bogdan
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Cutaneous Leishmaniasis ◽  
Leishmania Donovani ◽  
Leishmania Major ◽  
Parasite Burden ◽  
Skin Lesions ◽  
And Function ◽  
Oxide Synthase

ABSTRACT Cytokine-inducible (or type 2) nitric oxide synthase (iNOS) is indispensable for the resolution of Leishmania major or Leishmania donovani infections in mice. In contrast, little is known about the expression and function of iNOS in human leishmaniasis. Here, we show by immunohistological analysis of skin biopsies from Mexican patients with local (LCL) or diffuse (DCL) cutaneous leishmaniasis that the expression of iNOS was most prominent in LCL lesions with small numbers of parasites whereas lesions with a high parasite burden (LCL or DCL) contained considerably fewer iNOS-positive cells. This is the first study to suggest an antileishmanial function of iNOS in human Leishmania infections in vivo.

scholarly journals Serodiagnosis of Anthroponotic Cutaneous Leishmaniasis (ACL) Caused by Leishmania tropica in Sanliurfa Province, Turkey, Where ACL Is Highly Endemic

2007 ◽  
Vol 14 (11) ◽  
pp. 1409-1415 ◽  
Author(s):  
Fadile Yildiz Zeyrek ◽  
Metin Korkmaz ◽  
Yusuf Özbel
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Western Blotting ◽  
Blood Donors ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Serological Tests ◽  
Western Blot Test ◽  
Conventional Elisa ◽  
Negative Controls ◽  
Positive Controls

ABSTRACT In this study, we aimed to evaluate the validity of the conventional enzyme-linked immunosorbent assay (ELISA) and the Western blotting test for the diagnosis of anthroponotic cutaneous leishmaniasis (ACL) using serum samples obtained from 51 patients with parasitologically proven nontreated CL (NonT-CL patients) and 62 patients under treatment for CL (UT-CL patients). Additionally, 29 serum samples obtained from patients with parasitologically and serologically proven visceral leishmaniasis (VL) were also used as positive controls, and serum samples from 43 blood donors were used as negative controls. All sera were diluted to the same dilution (1/100). Leishmania infantum MON-1 was used as the antigen in the conventional ELISA. The sera of 27 (93.1%) of 29 VL patients were seropositive by ELISA, while the sera of 40 (78.4%) of 51 NonT-CL patients and 43 (69.3%) of 62 UT-CL patients were seropositive by the conventional ELISA. The absorbance values of the CL patients' sera were significantly lower than the absorbance values of the VL patients' sera. Bands between 15 and 118 kDa were detected in two groups of CL patients. Among all bands, the 63-kDa band was found to be more sensitive (88.5%). When we evaluated the Western blotting results for the presence of at least one of the diagnostic antigenic bands, the sensitivity was calculated to be 99.1%. By using serological tests, a measurable antibody response was detected in most of the CL patients in Sanliurfa, Turkey. It is also noted that this response can be changed according to the sizes, types, and numbers of lesions that the patient has. The Western blot test was found to be more sensitive and valid than the conventional ELISA for the serodiagnosis of ACL. In some instances, when it is very difficult to demonstrate the presence of parasites in the smears, immunodiagnosis can be a valuable alternative for the diagnosis of ACL.

Isolation and Molecular Diagnosis of Leishmania major and Study Activity of Aqueous Extract of Plant Nigella sativa Against the Parasite In Vitro

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Enas Abdul Kareem Jabbar ◽  
Bassad A. AL-Aboody ◽  
Basim Abdulhussein Jarullah ◽  
Nada Noori
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Molecular Diagnosis ◽  
Aqueous Extract ◽  
Teaching Hospital ◽  
Exposure Time ◽  
Leishmania Major ◽  
Nigella Sativa ◽  
Study Activity ◽  
Leishmania Spp

The current study included isolating and diagnosing the cutaneous leishmaniasis parasite from patients with Baghdad boil and the reviewers of Al Hussein Teaching Hospital in Thi-Qar province for the period from November to December 2016 and then studying the effect of aqueous extract of the plant Nigella sativa in the vitality of isolated parasite in vitro. Through the results, the isolated parasite was diagnosed depend on lesions appearance and characterization then confirm the diagnosis by KDNA- PCR which revealed that leishmania spp. was Leishmania major . In this study three different concentrations from aqueous extract of the Nigella sativa plant were used (50, 100 and 150 mg / ml), the parasite growing in NNN medium (3 replicates used)It was observed that the percentage of parasite destruction increased with prolong exposure time of the extract and increased concentration of the extract, results also exhibited that the percentage of the destruction was 100% after four hours of exposure to the concentration of 150 mg / ml which was the highly killed percentage compared to control, which was 5. 98%.

First Autochthonous Case of Emerged Zoonotic Cutaneous Leishmaniasis in Mazandaran Province, Northern Iran: kDNA-PCR Evidence Base

2020 ◽  
Vol 20 ◽  
Author(s):  
Fatemeh Niksolat ◽  
Rabeeh Tabaripour ◽  
Lotfollah Davoodi ◽  
Mahdi Fakhar
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Leishmania Major ◽  
Skin Lesions ◽  
Needle Aspiration ◽  
Evidence Base ◽  
Mazandaran Province ◽  
Northern Iran ◽  
Zoonotic Cutaneous Leishmaniasis ◽  
Smear Preparation ◽  
History Of

: In the last decade, several cases of cutaneous leishmaniasis (CL) have been recorded by the provincial health center of Mazandaran Province, northern Iran. However, there is no documented report of autochthonous CL in the province yet. The patient, a 59-year-old female, known case of diabetes mellitus, lived in rural area of Kiasar district (in eastern part of the Mazandaran) with 5 skin lesions suspected of having vasculitis, without history of traveling to the endemic areas of CL, was admitted to Imam Khomeini teaching hospital, Sari. To rule out CL, fine needle aspiration (FNA) procedure was used aseptically for the patient. Laboratory investigations showed Leishman bodies (amastigotes) by direct smear preparation and species identification confirmed Leishmania major (L. major) by polymerase chain reaction (PCR) molecular method. Accordingly, this is the first documented report of autochthonous CL in Mazandaran Province. Overall, due to the abundance of probable rodent reservoir hosts and confirmation of infection of sandflies (Phlebotomus papatasi) with L. major in the Kiasar district, where our patient was living there, it seems that CL emerged in the eastern part of the province. Therefore, further studies on rodents and sandflies fauna in terms of emerging leishmanial infection in the area is recommended.

scholarly journals Imported cutaneous leishmaniasis: molecular investigation unveils Leishmania major in Bangladesh

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Md Anik Ashfaq Khan ◽  
Rajashree Chowdhury ◽  
Rupen Nath ◽  
Sören Hansen ◽  
Progga Nath ◽  
...  
Keyword(s):  
Skin Biopsy ◽  
Cutaneous Leishmaniasis ◽  
Leishmania Donovani ◽  
Leishmania Major ◽  
Migrant Worker ◽  
Human Mobility ◽  
Skin Lesions ◽  
Careful Consideration ◽  
Molecular Tests ◽  
Imported Cases

Abstract Background The main clinical forms of leishmaniasis in Bangladesh are visceral leishmaniasis and post-kala-azar dermal leishmaniasis, which are caused by Leishmania donovani. Imported cutaneous leishmaniasis (CL) is emerging globally due mainly to increased human mobility. In recent years, several imported CL cases have also been reported in Bangladesh. Sporadic atypical cases of CL can be challenging for diagnosis and clinical management, while occurrence of infection on a frequent basis can be alarming. We report of a case of a Bangladeshi temporary-migrant worker who, upon return, presented development of skin lesions that are characteristic of CL. Methods A serum sample was collected and tested with an rK39 immunochromatographic test. Nucleic acid from skin biopsy derived culture sample was extracted and screened with a real-time PCR assay which targets the conserved REPL repeat region of L. donovani complex. The internal transcribed spacer 2 region of the ribosomal RNA gene cluster was amplified and sequenced. Results The suspect had a history of travel in both CL and VL endemic areas and had a positive rK39 test result. Based on clinical presentation, travel history and demonstration of the parasite in the skin biopsy, CL was diagnosed and the patient underwent a combination therapy with Miltefosine and liposomal amphotericin B. While typical endemic species were not detected, we identified Leishmania major, a species that, to our knowledge, has never been reported in Bangladesh. Conclusions Proper monitoring and reporting of imported cases should be given careful consideration for both clinical and epidemiological reasons. Molecular tests should be performed in diagnosis to avoid dilemma, and identification of causative species should be prioritized.

scholarly journals Sandfly Fever Sicilian Virus-Leishmania major co-infection modulates innate inflammatory response favoring myeloid cell infections and skin hyperinflammation

2021 ◽  
Vol 15 (7) ◽  
pp. e0009638
Author(s):  
Ellen Heirwegh ◽  
Emily MacLean ◽  
Jinlei He ◽  
Shaden Kamhawi ◽  
Selena M. Sagan ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Leishmania Major ◽  
Parasite Burden ◽  
Myeloid Cell ◽  
Skin Lesions ◽  
Health Concern ◽  
Global Public Health ◽  
Knock Out

Background The leishmaniases are a group of sandfly-transmitted diseases caused by species of the protozoan parasite, Leishmania. With an annual incidence of 1 million cases, 1 billion people living in Leishmania-endemic regions, and nearly 30,000 deaths each year, leishmaniasis is a major global public health concern. While phlebotomine sandflies are well-known as vectors of Leishmania, they are also the vectors of various phleboviruses, including Sandfly Fever Sicilian Virus (SFSV). Cutaneous leishmaniasis (CL), caused by Leishmania major (L. major), among other species, results in development of skin lesions on the infected host. Importantly, there exists much variation in the clinical manifestation between individuals. We propose that phleboviruses, vectored by and found in the same sandfly guts as Leishmania, may be a factor in determining CL severity. It was reported by our group that Leishmania exosomes are released into the gut of the sandfly vector and co-inoculated during blood meals, where they exacerbate CL skin lesions. We hypothesized that, when taking a blood meal, the sandfly vector infects the host with Leishmania parasites and exosomes as well as phleboviruses, and that this viral co-infection results in a modulation of leishmaniasis. Methodology/Principal findings In vitro, we observed modulation by SFSV in MAP kinase signaling as well as in the IRF3 pathway that resulted in a pro-inflammatory phenotype. Additionally, we found that SFSV and L. major co-infection resulted in an exacerbation of leishmaniasis in vivo, and by using endosomal (Toll-like receptor) TLR3, and MAVS knock-out mice, deduced that SFSV’s hyperinflammatory effect was TLR3- and MAVS-dependent. Critically, we observed that L. major and SFSV co-infected C57BL/6 mice demonstrated significantly higher parasite burden than mice solely infected with L. major. Furthermore, viral presence increased leukocyte influx in vivo. This influx was accompanied by elevated total extracellular vesicle numbers. Interestingly, L. major displayed higher infectiveness with coincident phleboviral infection compared to L. major infection alone. Conclusion/Significance Overall our work represents novel findings that contribute towards understanding the causal mechanisms governing cutaneous leishmaniasis pathology. Better comprehension of the potential role of viral co-infection could lead to treatment regimens with enhanced effectiveness.

scholarly journals Cutaneous Leishmaniasis – Dermoscopic Findings And Cryotherapy

Folia Medica ◽  
2015 ◽  
Vol 57 (1) ◽  
pp. 65-68 ◽  
Author(s):  
Hristo P. Dobrev ◽  
Desislava G. Nocheva ◽  
Dimitar Iv. Vuchev ◽  
Rumyana D. Grancharova
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Skin Lesions ◽  
Serological Tests ◽  
Non Invasive ◽  
Dermatological Examination ◽  
Invasive Method ◽  
Vascular Structures ◽  
Central Ulceration ◽  
The Right ◽  
Southern Greece

AbstractWe present a 60-year-old male patient who, three months after a holiday in Southern Greece, found a small ‘pimple’ on his back, which gradually got as big as a small walnut, the central part becoming ulcerated and scabby. Dermatological examination found an erythematous-to-livid nodular lesion on the right shoulder; it was 16 mm in diameter with central ulceration, covered with brownish crust which discharged pus-like secretion upon pressure. Microscope examination of Romanowsky-Giemsa stained lesion material detected amastigote forms ofLeishmania tropica. The culture investigation and serological tests for leishmaniasis were negative. Dermoscopy of the lesion found the following features: erythema, hyperkeratosis, central ulceration covered with brownish crust, “yellow tears-like” structures and “white starburst-like” patterns, and various vascular structures (including dotted vessels, comma-shaped vessels, hairpin- and glomerular-like vessels). The patient was diagnosed with cutaneous leishmaniasis and underwent four cryotherapy sessions every other week with excellent therapeutic results - complete resolution of infiltrate with subsequent gentle hypopigmented scarring. In conclusion, dermoscopy is an easily accessible non-invasive method which can be useful for the diagnosis of cutaneous leishmaniasis. Cryotherapy is the treatment of choice for single skin lesions.

scholarly journals Metabolite profiling of experimental cutaneous leishmaniasis lesions demonstrates significant perturbations in tissue phospholipids

2020 ◽  
Author(s):  
Adwaita R. Parab ◽  
Diane Thomas ◽  
Sharon Lostracco-Johnson ◽  
Jair Lage de Siqueira-Neto ◽  
James McKerrow ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Drug Targets ◽  
Leishmania Major ◽  
Neglected Tropical Diseases ◽  
Treatment Options ◽  
Skin Lesions ◽  
Wide Distribution ◽  
Tropical Diseases ◽  
Lesion Site

AbstractEach year 700,000 to 1.2 million new cases of cutaneous leishmaniasis (CL) are reported and yet CL remains one of thirteen diseases classified as neglected tropical diseases (NTDs). Leishmania major is one of several different species of that same genus that can cause CL. Current CL treatments are limited by adverse effects and rising resistance. Studying disease metabolism at the site of infection can lead to new drug targets. In this study, samples were collected from mice infected in the ear and footpad with L. major and analyzed by untargeted liquid chromatography-tandem mass spectrometry (LC-MS/MS). Significant differences in overall metabolite profiles were noted in the ear at the site of the lesion. Interestingly, lesion-adjacent, macroscopically healthy sites also showed alterations in specific metabolites, including select phosphocholines (PCs). Host-derived PCs in the lower m/z range (m/z 200-799) showed an increase with infection in the ear at the lesion site, while those in the higher m/z range (m/z 800-899) were decreased with infection at the lesion site. Overall, our results expanded our understanding of the mechanisms of CL pathogenesis through the host metabolism and may lead to new curative measures against infection with Leishmania.Author summaryCutaneous leishmaniasis (CL) is one of thirteen neglected tropical diseases in the world today. It is an infectious disease with a wide distribution spanning five continents, with increasing distribution expected due to climate change. CL manifests as skin lesions and ulcers that are disabling and stigmatized. With the current treatment options being limited, studying host-pathogen metabolism can uncover mechanisms of disease pathogenesis that may lead to new curative measures against infection. In this paper we used untargeted metabolomics to address molecular-level changes occurring in vivo in experimental skin lesions of Leishmania major. Distinct global metabolic profiles were observed. Total phosphocholines (PCs) and those in the lower m/z ranges were significantly higher at the site of the skin lesion in the ear. In addition, specific PCs as well as PCs of varied m/z ranges were also affected at healthy-appearing lesion-adjacent sites, indicating that infection-induced metabolic perturbations are not restricted to the lesion site. Ultimately, these results provide essential clues to the metabolic pathways affected by CL.

scholarly journals Cytokines, Signaling Pathways, and Effector Molecules Required for the Control of Leishmania (Viannia) braziliensis in Mice

2007 ◽  
Vol 75 (8) ◽  
pp. 3823-3832 ◽  
Author(s):  
F. Janaina Soares Rocha ◽  
Ulrike Schleicher ◽  
Jochen Mattner ◽  
Gottfried Alber ◽  
Christian Bogdan
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Leishmania Major ◽  
Skin Lesions ◽  
Interleukin 12 ◽  
Self Healing ◽  
Effector Molecules ◽  
Essential Components ◽  
Pathogen Control ◽  
Oxide Synthase ◽  
Signaling Components

ABSTRACT Cutaneous leishmaniasis is caused by protozoan parasites of the genus Leishmania. The mechanisms of pathogen control have been established primarily in the mouse model of Leishmania major infection, but they might not hold true for other Leishmania species associated with cutaneous disease. Here, we analyzed the role of cytokines, signaling components, and effector molecules in the control of New World cutaneous leishmaniasis due to L. braziliensis. Unlike L. major, L. braziliensis caused small, nonulcerative, and self-healing skin swelling in C57BL/6 mice, as well as BALB/c mice. In contrast to the results obtained for L. mexicana, mice deficient for interleukin-12 or its key signaling molecule, signal transducer and activator of transcription 4, rapidly succumbed to severe visceral leishmaniasis. Infection of tumor necrosis factor knockout mice with L. braziliensis led to progressive, nonhealing skin lesions with erosions and hemorrhagic ulcerations, but in contrast to the results with L. major, only 20 to 30% of the mice developed fatal visceral disease. As seen with L. major, mice with a deleted inducible nitric oxide synthase gene (iNOS−/−) were unable to contain L. braziliensis in the skin, whereas the control of the parasite in the spleen remained unimpaired. Unlike what happens in L. major infections, NADPH oxidase had no impact on the course of disease in L. braziliensis-infected mice. These results not only define essential components of a protective immune response to L. braziliensis but also illustrate that the requirements for the control of cutaneous leishmaniasis vary between different parasite species.

TBE in Sweden

2020 ◽  
Keyword(s):  
Genetic Characterization ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Second Phase ◽  
Serum Samples ◽  
Tick Borne Encephalitis ◽  
Specific Immunoglobulin ◽  
Tick Borne Encephalitis Virus ◽  
First Time

Tick-borne encephalitis virus (TBEV) was isolated for the first time in Sweden in 1958 (from ticks and from 1 tick-borne encephalitis [TBE] patient).1 In 2003, Haglund and colleagues reported the isolation and antigenic and genetic characterization of 14 TBEV strains from Swedish patients (samples collected 1991–1994).2 The first serum sample, from which TBEV was isolated, was obtained 2–10 days after onset of disease and found to be negative for anti-TBEV immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA), whereas TBEV-specific IgM (and TBEV-specific immunoglobulin G/cerebrospinal fluid [IgG/CSF] activity) was demonstrated in later serum samples taken during the second phase of the disease.

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