Carbonaceous and Protein Constituents in Dairy Wastewater Lead to a Differentiated Current Generation in Microbial Fuel Cells (MFCs)

The effect of real dairy wastewater (DWW) additions on the current density generated by a bioanode was evaluated in a half cell configuration under potentiostatic control, thus simulating the anodic chamber of a Microbial Fuel Cell. Low substrate additions increased current density up to 1655 ± 136 mA m-2, forming a two-current peak pattern. Then the system was tested with a casein-lactose synthetic media. A high protein concentration reduced the current density; individual compounds led to the highest current (330.5 mA m-2 with casein; 1276 mA m-2 with lactose). Moreover, the protein reduced the current start up time.

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Reduction of start-up time through bioaugmentation process in microbial fuel cells using an isolate from dark fermentative spent media fed anode

Water Science & Technology ◽

10.2166/wst.2015.174 ◽

2015 ◽

Vol 72 (1) ◽

pp. 106-115 ◽

Cited By ~ 12

Author(s):

Soumya Pandit ◽

Santimoy Khilari ◽

Shantonu Roy ◽

M. M. Ghangrekar ◽

Debabrata Pradhan ◽

...

Keyword(s):

Current Density ◽

Microbial Fuel Cells ◽

Electrochemical Impedance ◽

Pseudomonas Sp ◽

Ionization Time ◽

Start Up ◽

Electrochemically Active ◽

Electrochemical Impedance Spectroscopic ◽

Spent Media ◽

Isolated Species

Abstract An electrochemically active bacteria Pseudomonas aeruginosa IIT BT SS1 was isolated from a dark fermentative spent media fed anode, and a bioaugmentation technique using the isolated strain was used to improve the start-up time of a microbial fuel cell (MFC). Higher volumetric current density and lower start-up time were observed with the augmented system MFC-PM (13.7 A/m3) when compared with mixed culture MFC-M (8.72 A/m3) during the initial phase. This enhanced performance in MFC-PM was possibly due to the improvement in electron transfer ability by the augmented strain. However, pure culture MFC-P showed maximum volumetric current density (17 A/m3) due to the inherent electrogenic properties of Pseudomonas sp. An electrochemical impedance spectroscopic (EIS) study, along with matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) analysis, supported the influence of isolated species in improving the MFC performance. The present study indicates that the bioaugmentation strategy using the isolated Pseudomonas sp. can be effectively utilized to decrease the start-up time of MFC.

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Bioelectricity Generation from Wastewaters in Microbial Fuel Cells

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.512-515.1456 ◽

2012 ◽

Vol 512-515 ◽

pp. 1456-1460 ◽

Cited By ~ 1

Author(s):

Nipon Pisutpaisal ◽

Ubonrat Sirisukpoca

Keyword(s):

Fuel Cells ◽

Microbial Fuel Cells ◽

Oxygen Demand ◽

Half Cell ◽

Current Output ◽

Bioelectricity Generation ◽

Operation Temperature ◽

Anodic Chamber ◽

Cathodic Chamber ◽

Circuit Resistance

The study investigated bioelectricity generation from three types of wastewaters including artificial (AW), buffered brewery (BW) and buffered canteen (CW), in double chamber microbial fuel cells (MFCs). The biochemical oxygen demand (BOD) concentrations of influent were varied in the range of 125 - 1000 mg L-1. Influent pH and operation temperature were fixed at 7 and 30oC. 0.35 mL min-1wastewater was fed into a half-cell anodic chamber, while 5 mL min-1 oxygen-saturated distilled water was fed into a half-cell cathodic chamber. The circuit resistance was fixed at 10 ohms. The results showed that maximum current output obtained from AW, BW and CW with the initial BOD concentration of 1000 mg L-1were 0.92, 0.78 and 0.70 mA, respectively. The currents were directly proportional to the BOD concentrations in the influent for all wastewaters. The maximum BOD removal of AW, BW and CW was 90, 65 and 75%, respectively.

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Industrial wastewater treatment with a bioelectrochemical process: assessment of depuration efficiency and energy production

Water Science & Technology ◽

10.2166/wst.2017.532 ◽

2017 ◽

Vol 77 (1) ◽

pp. 134-144 ◽

Cited By ~ 20

Author(s):

Daniele Molognoni ◽

Stefania Chiarolla ◽

Daniele Cecconet ◽

Arianna Callegari ◽

Andrea G. Capodaglio

Keyword(s):

Microbial Fuel Cells ◽

Renewable Energy Sources ◽

Industrial Effluents ◽

Dairy Wastewater ◽

Innovative Technology ◽

Organic Loading Rate ◽

Research Issues ◽

Anodic Chamber ◽

Cathodic Chamber ◽

Better Than

Abstract Development of renewable energy sources, efficient industrial processes, energy/chemicals recovery from wastes are research issues that are quite contemporary. Bioelectrochemical processes represent an eco-innovative technology for energy and resources recovery from both domestic and industrial wastewaters. The current study was conducted to: (i) assess bioelectrochemical treatability of industrial (dairy) wastewater by microbial fuel cells (MFCs); (ii) determine the effects of the applied organic loading rate (OLR) on MFC performance; (iii) identify factors responsible for reactor energy recovery losses (i.e. overpotentials). For this purpose, an MFC was built and continuously operated for 72 days, during which the anodic chamber was fed with dairy wastewater and the cathodic chamber with an aerated mineral solution. The study demonstrated that industrial effluents from agrifood facilities can be treated by bioelectrochemical systems (BESs) with >85% (average) organic matter removal, recovering power at an observed maximum density of 27 W m−3. Outcomes were better than in previous (shorter) analogous experiences, and demonstrate that this type of process could be successfully used for dairy wastewater with several advantages.

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Cellulose Derived Graphene/Polyaniline Nanocomposite Anode for Energy Generation and Bioremediation of Toxic Metals via Benthic Microbial Fuel Cells

Polymers ◽

10.3390/polym13010135 ◽

2020 ◽

Vol 13 (1) ◽

pp. 135

Author(s):

Asim Ali Yaqoob ◽

Mohamad Nasir Mohamad Ibrahim ◽

Khalid Umar ◽

Showkat Ahmad Bhawani ◽

Anish Khan ◽

...

Keyword(s):

Fuel Cells ◽

Current Density ◽

Toxic Metals ◽

Microbial Fuel Cells ◽

Organic Substrate ◽

Synthetic Wastewater ◽

Multiple Parameters ◽

Pani Composite ◽

Industrial Level ◽

Modified Graphene

Benthic microbial fuel cells (BMFCs) are considered to be one of the eco-friendly bioelectrochemical cell approaches nowadays. The utilization of waste materials in BMFCs is to generate energy and concurrently bioremediate the toxic metals from synthetic wastewater, which is an ideal approach. The use of novel electrode material and natural organic waste material as substrates can minimize the present challenges of the BMFCs. The present study is focused on cellulosic derived graphene-polyaniline (GO-PANI) composite anode fabrication in order to improve the electron transfer rate. Several electrochemical and physicochemical techniques are used to characterize the performance of anodes in BMFCs. The maximum current density during polarization behavior was found to be 87.71 mA/m2 in the presence of the GO-PANI anode with sweet potato as an organic substrate in BMFCs, while the GO-PANI offered 15.13 mA/m2 current density under the close circuit conditions in the presence of 1000 Ω external resistance. The modified graphene anode showed four times higher performance than the unmodified anode. Similarly, the remediation efficiency of GO-PANI was 65.51% for Cd (II) and 60.33% for Pb (II), which is also higher than the unmodified graphene anode. Furthermore, multiple parameters (pH, temperature, organic substrate) were optimized to validate the efficiency of the fabricated anode in different environmental atmospheres via BMFCs. In order to ensure the practice of BMFCs at industrial level, some present challenges and future perspectives are also considered briefly.

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The Native Monomer of Bacillus Pumilus Ribonuclease Does Not Exist Extracellularly

BioMed Research International ◽

10.1155/2018/4837623 ◽

2018 ◽

Vol 2018 ◽

pp. 1-7 ◽

Cited By ~ 7

Author(s):

Olga Ilinskaya ◽

Vera Ulyanova ◽

Irina Lisevich ◽

Elena Dudkina ◽

Nataliya Zakharchenko ◽

...

Keyword(s):

Conformational Changes ◽

Protein Concentration ◽

Bacillus Pumilus ◽

Polyacrylamide Gel Electrophoresis ◽

Culture Fluid ◽

Size Exclusion ◽

High Protein Concentration ◽

Exclusion Chromatography ◽

Native Polyacrylamide Gel Electrophoresis ◽

Hypochromic Effect

Supported by crystallography studies, secreted ribonuclease of Bacillus pumilus (binase) has long been considered to be monomeric in form. Recent evidence obtained using native polyacrylamide gel electrophoresis and size-exclusion chromatography suggests that binase is in fact dimeric. To eliminate ambiguity and contradictions in the data we have measured conformational changes, hypochromic effect, and hydrodynamic radius of binase. The immutability of binase secondary structure upon transition from low to high protein concentration was registered, suggesting the binase dimerization immediately after translocation through the cell membrane and leading to detection of binase dimers only in the culture fluid regardless of ribonuclease concentration. Our results made it necessary to take a fresh look at the binase stability and cytotoxicity towards virus-infected or tumor cells.

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Challenges in the development of high protein concentration formulations

Journal of Pharmaceutical Sciences ◽

10.1002/jps.20079 ◽

2004 ◽

Vol 93 (6) ◽

pp. 1390-1402 ◽

Cited By ~ 565

Author(s):

Steven J. Shire ◽

Zahra Shahrokh ◽

Jun Liu

Keyword(s):

Protein Concentration ◽

High Protein ◽

High Protein Concentration

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AAC Richard Soybean

Canadian Journal of Plant Science ◽

10.1139/cjps-2021-0261 ◽

2022 ◽

Author(s):

Kangfu Yu ◽

Lorna Woodrow ◽

M. Chun Shi

Keyword(s):

Glycine Max ◽

Research And Development ◽

Soybean Cyst Nematode ◽

Protein Concentration ◽

Heterodera Glycines ◽

Cyst Nematode ◽

Processing Quality ◽

Food Grade ◽

High Protein Concentration ◽

Glycine Max L

AAC Richard is a food grade soybean [Glycine max (L.) Merr] cultivar with yellow hilum, high protein concentration, and good processing quality for foreign and domestic soymilk, tofu, and miso markets. It has resistance to SCN (soybean cyst nematode) (Heterodera Glycines Ichinohe). AAC Richard was developed at the Agriculture and Agri-Food Canada (AAFC) Harrow Research and Development Centre (Harrow-RDC), Harrow, Ontario and is adapted to areas of southwest Ontario with 3100 or more crop heat units and has a relative maturity of 2.3 (MG 2.3).

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