scholarly journals Production of Bioethanol from Dragon Fruit Wastes by Using Aspergillus niger and Saccharomycetes cerevisae

2022 ◽  
Author(s):  
Selvia Sarungu ◽  
Karnila Willard ◽  
Hamriani Ryka ◽  
Simon Tampang ◽  
Junaesar Tangke Tasik ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Dragon Fruit ◽  
Fruit Wastes

scholarly journals Screening of Fruit Wastes as Pectin Source

2017 ◽  
Vol 10 (1) ◽  
pp. 65-70
Author(s):  
R Begum ◽  
YA Yusof ◽  
MG Aziz ◽  
MB Uddin
Keyword(s):  
Natural Resources ◽  
Fruit Pulp ◽  
Waste Generation ◽  
Fruit Type ◽  
Pectin Content ◽  
Dragon Fruit ◽  
Calcium Pectate ◽  
Fruit Waste ◽  
Potential Sources ◽  
Fruit Wastes

This study was carried out on estimation of inedible part (waste) of fruit and screening for potential pectin content (calcium pectate) in fruit wastes with respect to type, location and maturity. Fruit waste generation was significantly different in different fruit type, variety and maturity. In a whole fruit, amount of fruit waste (peel & core) was significantly higher than amount of fruit pulp. Among the waste evaluated the overall calcium pectate producing potential in order of merit were lemon, dragon fruit, guava, mango, jackfruit, banana, pomegranate, papaya, watermelon and pineapple. Unused fruit waste could be potential sources of pectin production.J. Environ. Sci. & Natural Resources, 10(1): 65-70 2017

scholarly journals Bioethanol Levels of Dragon Fruit (Hylocereus polyrhizus) Peel with the Addition of Blend Crude Cellulase Enzyme from Trichoderma reesei and Aspergillus niger

2020 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Trianik Widyaningrum ◽  
Masreza Parahadi
Keyword(s):  
Aspergillus Niger ◽  
Trichoderma Reesei ◽  
Alternative Energy ◽  
Cellulose Degradation ◽  
Fruit Peel ◽  
Yeast Saccharomyces Cerevisiae ◽  
Fermentation Time ◽  
Dragon Fruit ◽  
Sugar Levels ◽  
Hylocereus Polyrhizus

The petroleum fuel crisis shows that Indonesia's fossil energy reserves are limited. It is necessary to develop an environmentally, friendly and sustainable alternative energy, one of which is bioethanol. This study aims to determine the bioethanol levels of dragon fruit (Hylocereus polyrhizus) peel with the treatment of cellulase enzymes from Trichoderma reesei and Aspergillus niger. This research was an experimental study that uses steps such as making dragon fruit peel substrate and filtrate, cellulose degradation with enzymes from Trichoderma reesei and Aspergillus niger and inoculating with yeast (Saccharomyces cerevisiae) with a fermentation time of 96 hours and then measured reducing sugar levels with the method of DNS, distillation, and the measurement of bioethanol levels using alcohol meters. The results have shown that using enzymes from Trichoderma reesei and Aspergillus niger can increase the reduction of 49.68 %  sugar levels in the treatment of T.reesei: A.niger (3: 1) and produce the highest bioethanol level, which is 2.46 % in the treatment of T.reesei: A.niger (2: 1)

scholarly journals Utilization of Fruit Waste for Bioethanol Production by Co-cultures of Aspergillus niger and Saccharomyces cerevisiae

2019 ◽  
pp. 63-72 ◽  
Author(s):  
Nutchanat Chamchoi Chamchoi
Keyword(s):  
Aspergillus Niger ◽  
Ethanol Yield ◽  
Maximum Yield ◽  
Banana Peel ◽  
Control Analysis ◽  
Erlenmeyer Flask ◽  
Pomelo Peel ◽  
Fermentation Temperature ◽  
Fruit Waste ◽  
Fruit Wastes

The purpose of this research was to study the effectiveness of simultaneous fermentation offruit waste with co-cultures of Aspergillus niger TISTR 3063 and Saccharomyces cerevisiaeTISTR 5606 in production of ethanol. The effect of fermentation temperature on ethanol yield was also observed. Pomelo and banana peels were selected as substrates and prepared by chopping into small rectangular pieces. Fermentation of batches of fruit waste was carriedout using a 250mL Erlenmeyer flask with glucose as a control. Analysis of the composition of the fruit waste included sugar, pH, TS, VS, ash, moisture, COD and TKN. From the results, it was found that maximum yields of 90.71% and 104.90% for pomelo and banana peel, respectively, were achieved at a temperature of 40°C within 24 h. The analysis also showed that fermentation temperature affected ethanol yield. When the fermentation temperature was raised from 30°C to 40°C, maximum ethanol yield from pomelo peel fermentation with10% of inoculum was increased from 73.86% to 90.71%, significant at p-0.05 Maximum yield from banana peel fermentation showed a similar trend. This study establishes the potential for upgrading fruit wastes such as pomelo and banana peels as high value substrates for ethanol production. Pomelo peel in particular shows high potential as a substrate for ethanol fermentation at 40°C for 24 h, with inoculum of 10% (w/w) of each fungus and yeast.

Fruit Wastes as Substrate For the Production of Amylase by Aspergillus niger

2017 ◽  
Vol 1 (4) ◽  
pp. 182-185
Author(s):  
Cyprian Oshoma ◽  
◽  
Sophia Eguakun-Owie ◽  
Solomon Omonigho ◽  
Henrietta Obueh ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Fruit Wastes

BIOLOGICAL CONTROL OF FUSARIUM WILT OF MUSKMELON BY FORMULATIONS OF ASPERGILLUS NIGER

1998 ◽  
Vol 46 (1) ◽  
pp. 67-72 ◽  
Author(s):  
Krishanu Mukherjee ◽  
Bineeta Sen
Keyword(s):  
Aspergillus Niger ◽  
Fusarium Wilt ◽  
Mass Production ◽  
Alternative Source ◽  
Colony Forming Units ◽  
Short Period ◽  
Cheap Alternative ◽  
Fruit Wastes ◽  
Fruit Pomace ◽  
Coating Formulation

Aspergillus niger van Tiegh. (An 27) was shown to be a promising biocontrol agent against Fusarium oxysporum Schlecht f. sp. melonis Sny. and Hans., which causes muskmelon wilt. Fruit wastes, such as carrot waste, citrus peels and pomace, mixed fruit pomace, and pineapple peels and pomace, proved to be a promising and cheap alternative source for mass multiplication of A. niger. These wastes are readily available throughout the year and can replace some of the highly priced media used for mass production of biocontrol organisms. Citrus pomace extract (20%) was found to be an excellent liquid medium for mass production. In solid fermentation, fruit wastes were superior to other media used as substrates. A short period of 10 days of incubation was sufficient for the production of 108 colony forming units (cfu) per gram of the material. The extent of control achieved by the seed coating formulation (FSC) was at par with Carbendazim. Formulation for soil application (FSO), a new contribution to the formulation science, could reduce Fusarium wilt disease by 78.31%, when applied at the rate of 8 g/kg of soil. Both FSC and FSO formulations can be stored at room temperature (14–35 °C) for more than a year.

Fungus ball paranasal sinuses: pattern of histopathology and culture characteristics

2019 ◽  
Vol 49 (20) ◽  
Author(s):  
Nugroho Suharsono
Keyword(s):  
Aspergillus Niger ◽  
Paranasal Sinus ◽  
Paranasal Sinuses ◽  
Histopathological Examination ◽  
Morphological Characteristics ◽  
Sinus Surgery ◽  
Nasal Discharge ◽  
Fungus Ball ◽  
Methenamine Silver ◽  
Fungus Balls

Background: Fungal infection of the nose and paranasal sinuses is an uncommon condition which is now being increasingly recognized. The clinical presentation is not specific with various symptoms such as nasal obstruction, purulent nasal discharge, facial pain, and chronic cough. Only unilaterality may alert the clinician. Purpose: To find the morphological characteristics of the fungus in patients with paranasal sinus fungus ball. Methods: A retrospective study of 13 paranasal sinus fungus balls cases which underwent endoscopic sinus surgery at Department of Otorhinolaryngology Head and Neck Surgery St. Vincentius A Paulo Hospital Surabaya from March, 2012 until December, 2013. Age, sex, histopathology and fungal cultur were analysed. Histopathologic sections of all the patients were stained with hematoxylin and eosin (H&E), and Gomori methenamine silver (GMS). The specimens were then cultured on Sabouraud dextrose agar plates and incubated at 30°C for 1 month. At the end of the incubation period, the samples were evaluated microscopically to detect fungi and identify their species. Results: The age reported of the 13 patients, was ranging from 36 to 63 years old. There was a significant female predominance, 10 female patients (76.92%) and 3 male patients (23.08%). Histopathological examination showed that most causative agents were Aspergillus species 92.31% (12/13). Culture test was positive for 69.23% (9/13). Aspergillus niger (61.54%, 8/13) is the most frequent fungus reported to cause fungus balls. Conclusion: Pattern of histopathologic on HE and GMS is very helpful and sensitive to identify fungi. The most common isolated mould in our study was Aspergillus niger.Keywords: fungus ball, histopathology and culture, Aspergillus nigerABSTRAK Latar Belakang: Infeksi jamur di hidung dan sinus paranasal merupakan kondisi yang jarang terjadi, namun kini lebih sering ditemukan. Gejala klinisnya tidak spesifik dapat berupa obstruksi hidung, sekret dari hidung, nyeri wajah, dan batuk kronis. Bila terjadi unilateral, patut diwaspadai oleh para klinisi. Tujuan: Untuk mengetahui karakteristik morfologi fungus yang didapati pada pasien sinusitis jamur yang kami teliti. Metode: Dilakukan penelitian retrospektif pada 13 pasien sinusitis jamur yang menjalani bedah sinus endoskopi di Departemen Otorinolaringologi-Kepala Leher Rumah Sakit St. Vincentius A Paulo Surabaya dari bulan Maret 2012 sampai dengan Desember 2013. Dilakukan analisis usia, jenis kelamin, histopatologi dan kultur jamur. Pewarnaan preparat histopatologi menggunakan Hematoxylin dan eosin (H&E) dan Gomori Methenamine Silver (GMS). Kemudian spesimen diletakkan pada piring agar Sabouraud dextrose, dan dilakukan inkubasi pada suhu 30°C selama satu bulan. Pada akhir masa inkubasi, sampel dievaluasi dengan mikroskop untuk mendeteksi jamur dan spesiesnya. Hasil: Didapati usia 13 penderita berkisar dari 36-63 tahun. Wanita lebih dominan sebanyak 10 penderita (76,92 %) dan 3 penderita laki-laki (23,08%). Hasil pemeriksaan histopatologi menunjukkan spesies Aspergillus sebagai penyebab utama (92,31%) pada 12 penderita (12/13).Tes kultur positif pada 69,23% (9/13). Jamur yang paling sering menyebabkan bola jamur pada sinus adalah Aspergillus niger (61,54%, 8/13). Kesimpulan: Pewarnaan preparat histopatologi menggunakan Hematoxylin dan eosin (H&E) dan Gomori Methenamine Silver (GMS) sangat berguna dan sensitif dalam mendeteksi adanya jamur. Jenis jamur yang paling banyak ditemukan pada penelitian kami adalah Aspergillus niger.

The Utilization of Red Dragon Fruit Peel Extract (Hylocereus costaricencis) As A Natural Colorant of Dried Noodle

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Silaturahmi Silaturahmi ◽  
Zaidiyah Zaidiyah ◽  
Tengku Mia Rahmiati
Keyword(s):  
Product Diversification ◽  
Fruit Peel ◽  
Physical Quality ◽  
Natural Colorant ◽  
Randomized Design ◽  
Solid Loss ◽  
Dragon Fruit ◽  
Completely Randomized Design ◽  
Peel Extract

The use of colorants in the dried noodle manufactures is an effort of product diversification. Besides of being used as a natural coloring agent, this peel extract is also used to improve nutritional value of the expected product. The purpose of this study was to determine the effect of red dragon fruit peel extract on the physical quality of dried noodle.  The study was conducted by using Completely Randomized Design (CRD) method with one factor, namely concentration of red dragon fruit peel extract (N1 = 10 ml, N2 = 15 ml, N3 = 20 ml, N4 = 25 ml, N5 = 30 ml).  The observations of its physical quality consisted of water absorption, solid loss during cooking, and organoleptic tests (aroma and color).  The best quality of dried noodle was obtained by using 30 ml red dragon fruit peel extract (N5) with physical properties, namely DSA levels of 351.92%, solid loss during cooking 4.78%, aroma 3.79 (like) and color 3.89 (like).

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