scholarly journals Utilization of Fruit Waste for Bioethanol Production by Co-cultures of Aspergillus niger and Saccharomyces cerevisiae

2019 ◽  
pp. 63-72 ◽  
Author(s):  
Nutchanat Chamchoi Chamchoi

The purpose of this research was to study the effectiveness of simultaneous fermentation offruit waste with co-cultures of Aspergillus niger TISTR 3063 and Saccharomyces cerevisiaeTISTR 5606 in production of ethanol. The effect of fermentation temperature on ethanol yield was also observed. Pomelo and banana peels were selected as substrates and prepared by chopping into small rectangular pieces. Fermentation of batches of fruit waste was carriedout using a 250mL Erlenmeyer flask with glucose as a control. Analysis of the composition of the fruit waste included sugar, pH, TS, VS, ash, moisture, COD and TKN. From the results, it was found that maximum yields of 90.71% and 104.90% for pomelo and banana peel, respectively, were achieved at a temperature of 40°C within 24 h. The analysis also showed that fermentation temperature affected ethanol yield. When the fermentation temperature was raised from 30°C to 40°C, maximum ethanol yield from pomelo peel fermentation with10% of inoculum was increased from 73.86% to 90.71%, significant at p-0.05 Maximum yield from banana peel fermentation showed a similar trend. This study establishes the potential for upgrading fruit wastes such as pomelo and banana peels as high value substrates for ethanol production. Pomelo peel in particular shows high potential as a substrate for ethanol fermentation at 40°C for 24 h, with inoculum of 10% (w/w) of each fungus and yeast.

2019 ◽  
Vol 9 (4-A) ◽  
pp. 9-14
Author(s):  
Subramaniyan Satheeshkumar ◽  
Paramasivam Sivagurunathan ◽  
Kannaiyan Muthulakshmi ◽  
Chinnaiyan Uma

The main organic acids in industrial used were citric, acetic, tartaric, malic, lactic and gluconic acid. The most utilized organic acid is citric acid or tricarboxylic acid. Chemical synthesis of citric acid is more costly than fermentation. The citrus processing industry generates tons of waste such as peel and segment membranes resulting from the extraction of citrus juice in industrial plants. About 6 isolates were obtained from the fruit waste dumped soil and they were subjected to screening for citric acid production. A. niger over other potential citric acid-producing organism towards efficient utilization of agroindustry residues and by-products for citric acid production. It was grown on PDA plates at a temperature of 28°C for 5 to 7 days. Fruit wastes like orange peel, sweet lime peel, banana peel and pineapple peel as a potential substrate for the production of citric acid using A. niger isolates. The nitrogen source of the fermentation medium will have a direct effect on the yield of citric acid. Among the four fruit wastes, citric acid production was maximum from orange peel followed by sweet lime peel, pineapple peel and banana peel respectively. Orange peel contains soluble sugars and pectin as the main components. Study the impact of different carbon sources on citric acid., the basal media were supplemented with glucose, sucrose, fructose, maltose and the fermentation were carried up to 5 days at 30°C. Nitrogen sources on the fermentation were studied by incorporating ammonium phosphate, potassium hydrogen phosphate and peptone. The effect of lower alcohol incorporation on the fermentation of citric acid was studied.


2019 ◽  
Vol 49 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Holly Hooper ◽  
Matthew J Grieshop

Abstract The arrival of spotted wing Drosophila, Drosophila suzukii (Matsumura), to the United States has caused many berry and cherry growers to replace IPM programs with calendar-based broad-spectrum insecticide programs. Alternative management tactics are urgently needed to mitigate the current dependency on chemical control. Postharvest burial is a cultural crop sanitation strategy that has the potential to reduce D. suzukii reproductive habitat and eliminate infested fruit wastes. This study revealed that D. suzukii rarely pupate on the soil’s surface or below 1 cm, but are capable of unburying themselves from depths up to 48 cm. Although zero emergence was not obtained in the field, adult emergence decreased exponentially with deeper burial depths. A burial depth of 24 cm reduced D. suzukii emergence by 97%, although soil texture may influence this optimal burial depth. Soils that had a higher concentration of sand had a negative impact on D. suzukii survival at shallower burial depths. The mechanism behind the reduction in adult emergence from differing burial depths remains unclear as the lipid concentration between emerging D. suzukii was the same regardless of burial depth.


2009 ◽  
Vol 75 (18) ◽  
pp. 5743-5749 ◽  
Author(s):  
Changhao Bi ◽  
Xueli Zhang ◽  
Lonnie O. Ingram ◽  
James F. Preston

ABSTRACT Dilute acid pretreatment is an established method for hydrolyzing the methylglucuronoxylans of hemicellulose to release fermentable xylose. In addition to xylose, this process releases the aldouronate methylglucuronoxylose, which cannot be metabolized by current ethanologenic biocatalysts. Enterobacter asburiae JDR-1, isolated from colonized wood, was found to efficiently ferment both methylglucuronoxylose and xylose in acid hydrolysates of sweet gum xylan, producing predominantly ethanol and acetate. Transformation of E. asburiae JDR-1 with pLOI555 or pLOI297, each containing the PET operon containing pyruvate decarboxylase (pdc) and alcohol dehydrogenase B (adhB) genes derived from Zymomonas mobilis, replaced mixed-acid fermentation with homoethanol fermentation. Deletion of the pyruvate formate lyase (pflB) gene further increased the ethanol yield, resulting in a stable E. asburiae E1(pLOI555) strain that efficiently utilized both xylose and methylglucuronoxylose in dilute acid hydrolysates of sweet gum xylan. Ethanol was produced from xylan hydrolysate by E. asburiae E1(pLOI555) with a yield that was 99% of the theoretical maximum yield and at a rate of 0.11 g ethanol/g (dry weight) cells/h, which was 1.57 times the yield and 1.48 times the rate obtained with the ethanologenic strain Escherichia coli KO11. This engineered derivative of E. asburiae JDR-1 that is able to ferment the predominant hexoses and pentoses derived from both hemicellulose and cellulose fractions is a promising subject for development as an ethanologenic biocatalyst for production of fuels and chemicals from agricultural residues and energy crops.


2012 ◽  
Vol 47 (1) ◽  
pp. 99-104 ◽  
Author(s):  
G Dharani ◽  
NS Kumaran

The purpose of this work is to study the optimized cultural conditions for the production of amylase by Aspergillus niger in solid state and submerged liquid fermentation. Four solid substrates banana peel, corn, potato and tapioca with different moisture conditions were taken for solid state fermentation (SSF). Basal medium was used for submerged liquid fermentation (SLF) with different pH (3 to 8), temperature (25, 30, 35 and 40ºC), carbon concentration (1, 2 and 3 g) and nitrogen source (0.1, 0.2 and 0.3 g). In SSF, tapioca yielded highest amylase activity and specific activity (4.43U/ml and 4.58U/mg) at 50% moisture content. In SLF, 2 g starch and 0.3 g peptone concentration showed 0.78 and 1.23 U/ml amylase activities under the optimum pH (5) and temperature (30ºC) the amylase activities reached to 0.86 U/ml and 0.76 U/ml respectively. In SSF using tapioca as substrate the enzyme yield is about five times higher than that achieved with submerged liquid culture. DOI: http://dx.doi.org/10.3329/bjsir.v47i1.7310 Bangladesh J. Sci. Ind. Res. 47(1), 99-104, 2012


Author(s):  
Ahmet Yusuf Şengül ◽  
Ömer Şengül ◽  
Aydın Daş

Particularly in the fruit juice industry, the remaining peels and pulp parts are not available for human consumption after the juices are used. It is possible to evaluate these wastes by adding them to poultry feeds. A number of studies have been conducted by researchers on the nutritional value of many fruit wastes and on the performance of poultry. Natural antioxidant content has been reported to have a positive effect on growth, microbial and immunological parameters when high fruit peels are used as feed additive in poultry feeding.


2020 ◽  
Vol 4 (2) ◽  
pp. 281-288
Author(s):  
O. O. Vinyukov ◽  
A. P. Dudkina ◽  
T. V. Shevchenko

Barley is a crop, requires the availability of available nutrients in the soil, especially at the be-ginning of the growing season. The aim of the research was to determine the effectiveness of the aftereffect of using vermicompost when growing spring barley on different backgrounds in the Donetsk region. The studies were carried out according to the methodology of the field experiment of B. A. Dospehova. Research methods: field, supplemented by analytical studies, measurements, calculations and observations. The studies were conducted in 2017–2019 by laboratory-field method in field crop rotation in the experimental sections of the Donetsk SSES NAAS. The repetition in the experiments is 3-fold. The location of the plots is systematic. The soil is alkaline-chernozemic carbonate, having an average supply of mineral nitrogen and mobile phosphorus, low – potassium. The humus content of 4,2 % indicates a high potential soil fertility, but to realize the potential of the culture, additional application of phosphorus-potassium fertilizers is necessary. The technology for growing crops is generally accepted for the farms of the region, with the ex- ception of the factors studied. Variety of barley spring Avers. The experience scheme provides for: control (without fertilizers); background – saturation of crop rotation with vermicompost granular 2 t/ha; background + N30P30K30; background + N60P60K60; N30P30K30. The use of organic fertilizers under the predecessor in crop rotation provided improve nutriti-on conditions for plants, which positively affected the growth and development of spring barley plants. So, on the variants with the use of mineral fertilizers, the highest indices of the number of productive stems and the structure of the crop were noted in the background. The variant where N60P60K60 was added in the background produced the largest mass of 1000 grains – 49,8 g, which is 4,6 % higher than the control. Analysis of the study indicates the positive effect of the aftereffect of organic fertilizer (ver-micompost) on the yield of spring barley. The maximum yield of spring barley was obtained against the background of aftereffect of vermicompost 2 t/ha and the application of mineral fertilizers N60P60K60 – 5,05 t/ha, but due to high production costs, the profitability level is 114,9 %. The application of organic fertilizers under the background provides a yield of 3,67 t/ha and the highest level of profitability of 140,6 %. The optimal technological option provides for the introduction of mineral fertilizers in the norm N30P30K30 amid predecessor saturation with vermicompost. This allows increasing the yield up to 4,90 t/ha (by 1,46 t/ha or 42,4 % to the control) and obtaining a profitability level of 136,1 %. The use only of mineral fertilizer N30P30K30 in severely arid weather conditions of the steppe zone allows increasing the yield by 0,70 t/ha compared to the control but, due to the high cost of pro-duction, leads to a low production profitability of 99,6 %. Key words: spring barley, background, vermicompost, mineral fertilizers, yield, economic effici-ency.


2022 ◽  
Author(s):  
Selvia Sarungu ◽  
Karnila Willard ◽  
Hamriani Ryka ◽  
Simon Tampang ◽  
Junaesar Tangke Tasik ◽  
...  

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