Study The Relationship Between Polymorphism of Melatonin Receptor Type 1A (MTNR1A) Gene with Some Reproductive Performance, in Local Iraqi Goats

2019 ◽  
Vol 43 (2) ◽  
pp. 38-41
Author(s):  
Imad Majeed Ali
Keyword(s):  
Restriction Enzyme ◽  
Reproductive Performance ◽  
Rflp Analysis ◽  
Melatonin Receptors ◽  
Receptor Type ◽  
Genotypic Frequency ◽  
Melatonin Receptor ◽  
Analysis Model ◽  
Hardy Weinberg Equilibrium ◽  
The Relationship

Melatonin is the main molecule that transmits the signal of seasonal change to the neuroendocrine system in seasonal breeding species, through specific melatonin receptors, Melatonin Receptor Type 1A. In the present study, a total of 40 local Iraqi non pregnant goats were used to study the relationship betweenPolymorphism of Melatonin Receptor Type 1A (MTNR1A) gene with some reproductive performance. Animals were reared in a farm located inAl-Yusufiya/Baghdad. Animals were divided based on breading season into two groups, with 20 animals each as follows: goats which gave birth in expected breading season (January, Febreuary,and March), and goats which gave birth in unexpected breading season (October, November, and December). All goats were reared in farm in Al-Yusufiya / Baghdad. 20 goats were given birth in January, February and March, which are representingseason, the other 0 goats which were given birth out of the breeding season during October, November and December, both tow groups were used to investigate the reproductive performance in local goats in different breeding months, the number of kids (single or twins), weight and sex of kids were record. Five milliliters of blood were collected from each goats through jugular vein puncture with EDTA coated tubes, until DNA extraction. The main part of exon II of MTNR1A gene was amplified by PCR with used specific primer and then product PCR digested with BsaI restriction enzyme. Therestriction fragment length polymorphism(RFLP) analysis used BsaI restriction enzyme, the presence of the cleavage site produces two fragments, 279 and 577 bp (C allele) while the absence of this site produces only one fragment, 856 bp (A allele).  Suggested the presence of three genotypes the homozygote CC for (279 bp, 577 bp), homozygote AA for (856 bp) and the heterozygote CA for (279 bp, 577 bp, 856 bp). In all the analyzed forty local goats’ genotypic frequency was 35.0% for CC genotype, 52.5% for CA genotype and 12.5 for AA genotype. The allele frequency of C and A was 61.25% and 38.75% respectively which was in Hardy–Weinberg equilibrium. The allele C have been associated with out of season breeding ability of goats and allele A associated with season breeding from the present study, no relationship between kids weights, sex and type of parturition (single or twin) with the genetic analysis model of MTNR1A gene in local Iraqi goats.

Association between melatonin receptor 1A (MTNR1A) gene polymorphism and the reproductive performance of Mediterranean Italian buffaloes

2012 ◽  
Vol 24 (7) ◽  
pp. 983 ◽  
Author(s):  
S. Luridiana ◽  
M. C. Mura ◽  
M. Pazzola ◽  
M. Paludo ◽  
G. Cosso ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Genetic Marker ◽  
Breeding Season ◽  
Reproductive Performance ◽  
Reproductive Activity ◽  
Allelic Frequency ◽  
Genotypic Frequency ◽  
Melatonin Receptor ◽  
Reproductive Seasonality ◽  
Long Photoperiod

A melatonin receptor 1A (MTNR1A) gene polymorphism in adult buffaloes has been reported to affect reproductive seasonality. Consequently, the aim of the present study was to assess whether this polymorphism can affect age at first conception and the interval between first and second calving in Mediterranean Italian buffaloes. The allelic frequency of the C and T alleles was 0.44 and 0.56, respectively, whereas the genotypic frequency was 26% for C/C, 40% for C/T and 34% for T/T. The average age at first mating was approximately 20 months, whereas that at calving was approximately 32 months. The largest number of calvings of primiparous buffaloes was recorded between June and October. No associations between genotype, first mating and subsequent calving date were found. The duration from first to second calving was longer in buffaloes with the C/C genotype compared with those with the T/T and C/T genotypes (P < 0.01). The period of calving for buffaloes with the C/C genotype was mainly from July to September, whereas that for buffaloes with the T/T genotype was largely from March to May. The MTNR1A gene had no effect on the age of first conception in Mediterranean Italian buffaloes. Rather, the association between the T/T genotype and reproductive activity during days with a long photoperiod indicates that this polymorphism may be considered a genetic marker to identify buffaloes that are able to reproduce out of the breeding season.

Melatonin Receptor as a Drug Target for Neuroprotection

2020 ◽  
Vol 14 (2) ◽  
pp. 150-164 ◽  
Author(s):  
Pawaris Wongprayoon ◽  
Piyarat Govitrapong
Keyword(s):  
The Body ◽  
Melatonin Receptors ◽  
Receptor Type ◽  
Substantia Nigra Pars Compacta ◽  
Melatonin Receptor ◽  
Amyloid Aβ ◽  
Disease Modifying Agents ◽  
Cerebral Ischemic ◽  
Cerebral Ischemic Reperfusion

Background:: Melatonin, a neurohormone secreted from the pineal gland, circulates throughout the body and then mediates several physiological functions. The pharmacological effects of melatonin can be mediated through its direct antioxidant activity and receptor-dependent signaling. Objective: This article will mainly review receptor-dependent signaling. Human melatonin receptors include melatonin receptor type 1 (MT1) and melatonin receptor type 2 (MT2), which are widely distributed throughout the brain. Result: Several lines of evidence have revealed the involvement of the melatonergic system in different neurodegenerative diseases. Alzheimer’s disease pathology negatively affects the melatonergic system. Melatonin effectively inhibits β-amyloid (Aβ) synthesis and fibril formation. These effects are reversed by pharmacological melatonin receptor blockade. Reductions in MT1 and MT2 expression in the amygdala and substantia nigra pars compacta have been reported in Parkinson’s disease patients. The protective roles of melatonin against ischemic insults via its receptors have also been demonstrated. Melatonin has been reported to enhance neurogenesis through MT2 activation in cerebral ischemic/reperfusion mice. The neurogenic effects of melatonin on mesenchymal stem cells are particularly mediated through MT2. Conclusion: Understanding the roles of melatonin receptors in neuroprotection against diseases may lead to the development of specific analogs with specificity and potency greater than those of the original compound. These successfully developed compounds may serve as candidate preventive and disease-modifying agents in the future.

scholarly journals Activation of MT2 melatonin receptors in rat suprachiasmatic nucleus phase advances the circadian clock

2001 ◽  
Vol 280 (1) ◽  
pp. C110-C118 ◽  
Author(s):  
Amanda E. Hunt ◽  
Walid M. Al-Ghoul ◽  
Martha U. Gillette ◽  
Margarita L. Dubocovich
Keyword(s):  
Circadian Clock ◽  
Firing Rate ◽  
Suprachiasmatic Nucleus ◽  
Neuronal Firing ◽  
Melatonin Receptors ◽  
Receptor Type ◽  
Melatonin Receptor ◽  
Specific Expression ◽  
Circadian Phase ◽  
Neuronal Firing Rate

The aim of this study was to identify the melatonin receptor type(s) (MT1 or MT2) mediating circadian clock resetting by melatonin in the mammalian suprachiasmatic nucleus (SCN). Quantitative receptor autoradiography with 2-[125I]iodomelatonin and in situ hybridization histochemistry, with either 33P- or digoxigenin-labeled antisense MT1 and MT2 melatonin receptor mRNA oligonucleotide probes, revealed specific expression of both melatonin receptor types in the SCN of inbred Long-Evans rats. The melatonin receptor type mediating phase advances of the circadian rhythm of neuronal firing rate in the SCN slice was assessed using competitive melatonin receptor antagonists, the MT1/MT2nonselective luzindole and the MT2-selective 4-phenyl-2-propionamidotetraline (4P-PDOT). Luzindole and 4P-PDOT (1 nM-1 μM) did not affect circadian phase on their own; however, they blocked both the phase advances (∼4 h) in the neuronal firing rate induced by melatonin (3 pM) at temporally distinct times of day [i.e., subjective dusk, circadian time (CT) 10; and dawn, CT 23], as well as the associated increases in protein kinase C activity. We conclude that melatonin mediates phase advances of the SCN circadian clock at both dusk and dawn via activation of MT2 melatonin receptor signaling.

The relationship between melatonin receptor 1A gene (MTNR1A) polymorphism and reproductive performance in Sarda breed sheep

2015 ◽  
Vol 171 ◽  
pp. 78-83 ◽  
Author(s):  
S. Luridiana ◽  
M.C. Mura ◽  
C. Daga ◽  
M.L. Diaz ◽  
P.P. Bini ◽  
...  
Keyword(s):  
Reproductive Performance ◽  
Melatonin Receptor ◽  
The Relationship ◽  
Breed Sheep

scholarly journals Influential Path of Social Risk Factors toward Suicidal Behavior—Evidence from Chinese Sina Weibo Users 2013–2018

2021 ◽  
Vol 18 (5) ◽  
pp. 2604
Author(s):  
Yujin Han ◽  
He Li ◽  
Yunyu Xiao ◽  
Ang Li ◽  
Tingshao Zhu
Keyword(s):  
Risk Factors ◽  
Path Analysis ◽  
Suicide Prevention ◽  
Suicidal Behavior ◽  
Empirical Support ◽  
National Bureau ◽  
Social Risk ◽  
Analysis Model ◽  
The Relationship ◽  
Path Analysis Model

(1) Purpose: The purpose of this study was to determine suicidal risk factors, the relationship and the underlying mechanism between social variables and suicidal behavior. We hope to provide empirical support for the future suicide prevention of social media users at the social level. (2) Methods: The path analysis model with psychache as the mediate variable was constructed to analyze the relationship between suicidal behavior and selected social macro variables. The data for our research was taken from the Chinese Suicide Dictionary, Moral Foundation Dictionary, Cultural Value Dictionary and National Bureau of Statistics. (3) Results: The path analysis model was an adequate representation of the data. With the mediator psychache, higher authority vice, individualism, and disposable income of residents significantly predicted less suicidal behavior. Purity vice, collectivism, and proportion of the primary industry had positive significant effect on suicidal behavior via the mediator psychache. The coefficients of harm vice, fairness vice, ingroup vice, public transport and car for every 10,000 people, urban population density, gross domestic product (GDP), urban registered unemployment rate, and crude divorce rate were not significant. Furthermore, we applied the model to three major economic development belts in China. The model’s result meant different economic zones had no influence on the model designed in our study. (4) Conclusions: Our evidence informs population-based suicide prevention policymakers that incorporating some social factors like authority vice, individualism, etc. can help prevent suicidal ideation in China.

Research on Related Model for Urban Public Crisis Handle Mechanism

2014 ◽  
Vol 989-994 ◽  
pp. 5540-5543
Author(s):  
Yong Chang Ren
Keyword(s):  
Crisis Management ◽  
Evaluation Model ◽  
Point Of View ◽  
Theoretical Point ◽  
Analysis Model ◽  
The Public ◽  
Public Crisis ◽  
Crisis Events ◽  
Response And Recovery ◽  
The Relationship

China is in a critical period of urbanization, and various social contradictions continue to be accumulated, emerged and enlarged, so public crisis management mechanism has been highly valued by governments at all levels with the public crisis events are occurred frequently. The paper conducts study for the problems in the current urban public crisis handle mechanism. First, the evaluation model of crisis management can be researched, and crisis management can be divided into four stages to evaluate respectively, they are Reduction, Readiness, Response and Recovery; then, we should research crisis prediction model to strengthen prediction, prevention and monitoring of the crisis before the crisis happened; finally, stakeholders analysis model should be studied, and scientific analyzing the interests of the various stakeholders and the relationship among them. From the theoretical point of view, the paper carries on a study for crisis handle mechanism to provide support for improving the crisis handle level.

​Identification of Single Nucleotide Polymorphism (SNP) C-2161G in the Promoter Region of Prolactin Gene and its Association with Egg Production in Tellicherry Native Chicken

2021 ◽  
Author(s):  
Azhaguraja Manoharan ◽  
S. Sankaralingam ◽  
P. Anitha ◽  
Binoj Chacko ◽  
T.V. Aravindakshan
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Promoter Region ◽  
Egg Production ◽  
Rflp Analysis ◽  
Genotypic Frequency ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Chicken Population ◽  
Native Chicken ◽  
Prolactin Gene

Background: Prolactin is a physiological candidate gene which has significant effects on egg production in poultry. Also, it plays a major role on incubation behaviour (broodiness) in birds. The main objective of the present study was identification of single nucleotide polymorphism (SNP) C-2161G in the promoter region of prolactin gene and its association with egg production in Tellicherry native chicken population. Methods: A total of 200 blood samples were collected from the randomly selected birds of Tellicherry native chicken in All India Co-ordinated Research Project on Poultry improvement (AICRP) farm, Mannuthy, Thrissur, Kerala. Isolation of Genomic DNA was done and the isolated samples were subjected to polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analysis to identify the SNP C-2161G of prolactin gene. Result: On the basis of RFLP patterns, birds were designated with three different genotypes namely CC, CG and GG. The allelic and genotypic frequency was calculated. The observed genotypic frequency at the SNP site C-2161G was CC (0.61), CG (0.160) and GG (0.23) and the frequency of allele was 0.69 for C and 0.31 for G. The egg production was shown to be statistically similar for the genotypes of SNP C-2161G.

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