Optimization of carbon source for hydrogen production by Bacillius licheniformis

Present study is an attempt to explore natural mix algae as a source of bio-hydrogen which is a renewable and environmental friendly clean fuel. Idea is to produce hydrogen in two stages; in first stage algae biomass is grown in light, oxygen and carbon source. In the second stage grown algae produce hydrogen in the absence of light, oxygen and sulfur. Results obtained during study showing that by using natural mix algae hydrogen up to 520 ml/l of algae biomass can be produce in one cycle. However, hydrogen gas can be produced in sufficient quantity up to three cycles. Biological hydrogen production with unicellular mix algae, found to be a promising area of research and conceptual by nature in the laboratory. This process needs to be explored more to prove the ability to use at commercial level. Still much work is required as to make process more economical and practical.

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Hydrogen Production from Synthesis Gas Using the Photosynthetic Bacterium Rhodospirillum rubrum

ASEAN Journal of Chemical Engineering ◽

10.22146/ajche.50162 ◽

2005 ◽

Vol 5 (1) ◽

pp. 35

Author(s):

Ghasem Najafpour ◽

Habibollah Younesi

Keyword(s):

Mass Transfer ◽

Carbon Source ◽

Hydrogen Production ◽

Synthesis Gas ◽

Rhodospirillum Rubrum ◽

Anaerobic Bacteria ◽

Photosynthetic Bacterium ◽

Attractive Alternative ◽

Agitation Rate ◽

Acetate Concentration

Production of biological hydrogen by anaerobic photosynthetic bacteria, specifically Rhodospirillum rubrum, from synthesis gas was successfully conducted at ambient temperature and pressure. The influence of initial acetate concentration as the substrate for microbial growth was investigated in a batch system. Series of experiments were conducted using serum bottles as bioreactor. The agitation rate and light intensity were adjusted at 200 rpm and 1,000 lux, respectively. The concentration of acetate as carbon source was varied from 0.5 to 3.0 g/I. It was observed that the increase in concentration of the carbon source from 2.5 to 3 g/l resulted in the decrease both in the growth of the microorganism and in hydrogen production rate. Experimental results showed that the optimum acetate concentration would be from 1 to 2 g/I. The resulting data also showed that in 1-2 g/I acetate, highest hydrogen formation and cell concentration were obtained. Additional acetate in the initial culture medium inhibited the growth of R. rubrum. An inverse relationship between acetate concentration and initial cell growth was observed. This article presents a method to calculate the mass transfer coefficient for gaseous substrates and the process parameters involved in a gas and liquid fermentation system. The procedure had been defined by experimental data for the bioconversion of CO to C02' while H20 is converted into hydrogen. Hence, a biologically-based water-gas shift reaction provided an attractive alternative improvement for renewable resources to achieve higher hydrogen production. Keywords: Anaerobic bacteria, batch culture, coefficient, CO uptake rate, mass transfer, photobiological hydrogen, and Rhodospirillum rubrum.

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Facile process to utilize carbonaceous waste as a carbon source for the synthesis of low cost electrocatalyst for hydrogen production

International Journal of Hydrogen Energy ◽

10.1016/j.ijhydene.2019.11.239 ◽

2020 ◽

Vol 45 (44) ◽

pp. 23908-23919

Author(s):

Rameez Ahmad Mir ◽

Gurbinder Kaur ◽

O.P. Pandey

Keyword(s):

Carbon Source ◽

Hydrogen Production ◽

Low Cost

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Characteristics of Biohydrogen Production by Ethanoligenens Sp. R3 Isolated from Continuous Stirred Tank Reactor

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.152-153.702 ◽

2010 ◽

Vol 152-153 ◽

pp. 702-707

Author(s):

An Ying Jiao ◽

Yong Feng Li ◽

Bing Liu ◽

Kun Liu ◽

Jing Li Xu

Keyword(s):

Carbon Source ◽

Hydrogen Production ◽

Nitrogen Source ◽

Ph Value ◽

Stirred Tank ◽

Continuous Stirred Tank Reactor ◽

Stirred Tank Reactor ◽

Tank Reactor ◽

Initial Ph ◽

Continuous Stirred Tank

Ethanoligenens sp. R3 isolated from anaerobic sludge in a continuous stirred tank reactor was investigated for its characteristics of fermentative hydrogen production in this study. Effects of initial pH value, fermentation temperature and proportion of carbon/nitrogen source on hydrogen production were investigated in batch experiments. The typical substrate for hydrogen production of glucose and peptone were used as the carbon source and nitrogen source, respectively. The results show that the maximum hydrogen production yield of 834 mlH2/L culture was obtained with initial pH value of 5.5 and fermentation temperature at 35°C. In addition, the maximum average hydrogen production rate of 10.87mmolH2/g cell dry weighth was achieved at the carbon/nitrogen source ratio of 3.3. The degradation efficiency of the glucose used as the carbon source ranged from 91.5% to 95.43% during the conversion of glucose to hydrogen by bacteria Ethanoligenens sp. R3.

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Effect of Pre-Treatment Palm Oil Mill Effluent POME on Biohydrogen Production by Local Isolate Clostridium Butyricum

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.236-238.2987 ◽

2011 ◽

Vol 236-238 ◽

pp. 2987-2992 ◽

Cited By ~ 3

Author(s):

Syafawati Ahmad Kamal ◽

Mariatul Fadzillah Mansor ◽

Jamaliah Mohd Jahim ◽

Nurina Anuar

Keyword(s):

Heat Treatment ◽

Carbon Source ◽

Hydrogen Production ◽

Palm Oil ◽

Reducing Sugar ◽

Palm Oil Mill Effluent ◽

Total Carbohydrate ◽

Initial Ph ◽

Palm Oil Mill ◽

Pre Treatment

Palm oil mill effluent (POME) contains approximately 6% fiber. The effectiveness of pre-treatment on POME can serve a very good feedstock for hydrogen production in fermentation process. In this research, the effectiveness of pre-treatment methods on POME treated using acid and base were analysed based total carbohydrate and reducing sugar content. By using 1M NaOH with heat treatment, 26.12% carbon source converted to reducing sugar while by using 1M H2SO4 with heat treatment, over 32.09% carbon source converted to reducing sugar. The highest increment of total carbohydrate where from acid-heat treatment with 26.1% increment from initial concentration. At the initial pH (5.5) with fermentation temperature 37°C, the highest hydrogen production rate given by acid-heat treatment was 0.5mL H2/mL POME. Different for initial pH 7.0 with the same temperature, the highest hydrogen produced rate was given by base-heat treatment with 0.59 mL H2/mL POME. The production of hydrogen in 2L bioreactor given much higher hydrogen production compare to production in serum bottle. This fermentation was run in batch mode with initial pH 7 and control at 5.5. The maximum hydrogen produce was 4304 mL H2/ L POME from acid-heat treatment.

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Anaerobic fermentation combined with low-temperature thermal pretreatment for phosphorus-accumulating granular sludge: Release of carbon source and phosphorus as well as hydrogen production potential

Bioresource Technology ◽

10.1016/j.biortech.2016.06.060 ◽

2016 ◽

Vol 218 ◽

pp. 18-26 ◽

Cited By ~ 24

Author(s):

Jinte Zou ◽

Yongmei Li

Keyword(s):

Low Temperature ◽

Carbon Source ◽

Hydrogen Production ◽

Anaerobic Fermentation ◽

Granular Sludge ◽

Thermal Pretreatment ◽

Production Potential

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Morphological Characterization of Mycobacteriophage R1

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051281 ◽

1974 ◽

Vol 32 ◽

pp. 254-255

Author(s):

B. L. Soloff ◽

T. A. Rado

Keyword(s):

Carbon Source ◽

Stationary Phase ◽

Growth Phase ◽

Minimal Medium ◽

Morphological Characterization ◽

Logarithmic Growth Phase ◽

Complete Lysis ◽

Lysogenic Culture ◽

Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

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