scholarly journals PENGARUH 2,4- D TERHADAP PENINGKATAN KUALITAS DAN KUANTITAS KALUS YANG DIINDUKSI DENGAN KOMBINASI AUKSIN DAN SITOKININ PADA KULTUR DAUN RUMPUT BENGGALA ( Panicum maximum) VARIETAS TRICHOGLUME

2021 ◽  
Vol 9 (1) ◽  
pp. 27-35
Author(s):  
Marna Eoh
Keyword(s):  
Acetic Acid ◽  
Growth Regulator ◽  
Callus Induction ◽  
Panicum Maximum ◽  
Standard Medium ◽  
Leaf Culture ◽  
Friable Callus ◽  
Brown Colour ◽  
Callus Production

To determine the effect various concentration 2, 4, 6 and 8 mg/l of 2,4- D (2,4- dicloropenoxy acetic acid) on callus induction of Benggala grass variety Trichoglume leaf culture on Murashige and Skoog (MS) standard medium and its organogenesis stimulated using deffent concentration of growth regulator, namely [N6- (2- isopenteny)- adenine] or 2iP (0, 0.2 and 1.0 mg/l ) and (l- naphthalene acetic ent, wacid) or NAA (0, 0.03, 0.16 and 3.0 mg/l) were performed. Percentage of callus were measured and organogenesis from callus were subjected to description analysis . The results showed that callus induction was optimum when 2,4-D was treated at 4 mg/l, friable callus were produced. Percentage production of callus week 4 was 46.2 percent, while using 8 mg/l 2,4 –D the callus production was about 59.7 percent yellowish coloured and more compact callus were produced. Combination of 0 mg/l 2iP ( auxin) +  3mg/l   (cytokinin) at 3 weeks showed reseilted 100 % of calluses produced roots, the highest amaunt roots (9,0) was observed in combination 0 mg/l 2iP + 3 mg/l NAA, and the longest root (17,0 mm) was recorded in combination 0 mg/l 2iP + 3 mg/l NAA. Calluses yielded  varied from white, yellowish to brown colour.

scholarly journals Factors Affecting Callus Induction and Organogenesis in Saffron (Crocus sativus L.)

2014 ◽  
Vol 24 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Maryam Vahedi ◽  
Siamak Kalantari ◽  
Seyed Alireza Salami
Keyword(s):  
Shoot Regeneration ◽  
Growth Regulator ◽  
Callus Induction ◽  
Growth Parameters ◽  
Crocus Sativus ◽  
Factors Affecting ◽  
Callus Production ◽  
Planting Materials ◽  
Disease Free ◽  
Crocus Sativus L

Saffron (Crocus sativus L.) belongs to Iridaceae and is known an important native commercial plants in Iran for its high value of saffron. The best growth regulator composition for callus production from corms and sahoot regeneration from callus were determined. Saffron corms harvested from previous crops are generally used for future cropping cycles. However, this practice causes major yield losses due to the heavy attack by different pathogens. Availability of healthy disease free planting materials is of great importance for successful cultivation of saffron. By this investigation the best composition of growth regulators for callus production from corms and shoot regeneration from callus were determined. Callus induction of Crocus sativus L. was investigated by using different combinations NAA, 2, 4-D and TDZ, BA and Kn. The highest frequency of callus induction was observed in medium containing 2 mg/l 2, 4-D + 1 mg/l BA followed by 1 mg/l 2, 4-D + 0.15 mg/l Kn. However, in case of growth parameters such as diameter and the area of calli the best result was obtained in the medium supplemented with 2 mg/l 2, 4-D + 1 mg/l BA.  In some treatments, calli were transferred to organogenesis stage after two subcultures.  For sprouting of shoots transferred to medium containing 1 mg/l BAP and 1 mg/l NAA. Statistical analyses indicated that the treatment containing 5 mg/l NAA and 5 mg/l TDZ  proved to be the best growth regulator treatment for shoot regeneration from the saffron calli. Plant Tissue Cult. & Biotech. 24(1): 1-9, 2014 (June) D. O. I. http://dx.doi.org/10.3329/ptcb.v24i1.19184

scholarly journals Induksi Kalus Piper retrofractum Vahl. dengan Zat Pengatur Tumbuh Auksin dan Sitokinin

2018 ◽  
Vol 3 (2) ◽  
pp. 41-46
Author(s):  
Junairiah . ◽  
Dewi Amelia Sofiana ◽  
Yosephine Sri Wulan Manuhara ◽  
Surahmaida .
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Secondary Metabolites ◽  
Growth Regulator ◽  
Plant Tissue Culture ◽  
Callus Induction ◽  
Qualitative Data ◽  
Dry Weight ◽  
Naphthalene Acetic Acid ◽  
Piper Retrofractum

ABSTRAK Cabai Jawa (Piper retrofractum Vahl.) dikenal sebagai tanaman hias dan tanaman obat. Metabolit sekunder pada tanaman ini adalah piperin, saponin, kavisin dan minyak atsiri. Metabolit sekunder  tersebut dapat diisolasi dari bahan tanaman atau kalus hasil kultur jaringan tanaman. Pada metode kultur jaringan  tanaman untuk menginduksi kalus diperlukan media dengan konsentrasi zat pengatur tumbuh yang tepat untuk mendapatkan hasil yang optimal. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi konsentrasi zat pengatur tumbuh Naphthalene Acetic Acid (NAA) dan 6-Benzyl Amino Purin (BAP) yang paling baik untuk induksi kalus P. retrofractum. Jenis penelitian ini adalah eksperimental laboratoris. Penelitian menggunakan rancangan acak lengkap (RAL) dengan 17 perlakuan yang terdiri atas 16 perlakuan kombinasi zat pengatur tumbuh dan 1 perlakuan kontrol, Setiap perlakuan terdiri atas  6 ulangan. Eksplan daun P. retrofractum ditumbuhkan pada medium Murashige dan Skoog padat ditambah  dengan zat pengatur tumbuh dengan konsentrasi masing-masing 0; 0,5; 1; 1,5; 2 mg/L. Data yang diperoleh  berupa data kuantitatif dan kualitatif. Data kuantitatif meliputi lama waktu induksi kalus, persentase eksplan membentuk kalus, berat segar kalus dan berat kering kalus, dianalisis secara statistik dengan SPSS. Data kualitatif meliputi warna dan tekstur kalus. Hasil penelitian menunjukkan bahwa zat pengatur tumbuh NAA dan BAP berpengaruh terhadap pertumbuhan eksplan daun P. retrofractum. Penambahan kombinasi konsentrasi NAA 0,5 mg/L dan BAP 0,5 mg/L menunjukkan respon terbentuknya kalus paling cepat yaitu 11,5 hari. Penambahan kombinasi konsentrasi NAA 1 mg/L dan BAP 0,5 mg/L menghasilkan berat segar terbaik  yaitu 70,6 mg, sedangkan pada kombinasi konsentrasi  NAA 1 mg/L dan BAP 2 mg/L menghasilkan berat kering terbaik  yaitu 18 mg. Warna kalus adalah putih dan putih kecokelatan dengan tekstur friabel dan kompak.Kata kunci: BAP, induksi kalus, NAA, Piper retrofractum Vahl.ABSTRACT Chili Java (Piper retrofractum Vahl.) is known as ornamental plants and medicinal plants. Secondary metabolites in this plant are piperin, saponin, kavisin and essential oils. Secondary metabolites can be isolated from plant material or callus from plant tissue culture. In plant tissue culture method to induce callus required media with the growth regulator concentration to get optimal result. The aim of this research is to know the effect of the combination of growth regulator of Naphthalene Acetic Acid (NAA) and 6-Benzyl Amino Purin (BAP) which is best for the induction of P. retrofractum callus. The type of this study was laboratory experimental. The study used a complete randomized design (RAL) with 17 treatments consisting of 16 treatment combinations of growth regulators and 1 control treatment. Each treatment consisted of 6 replications. P. retrofractum leaf eksplan grown on Murashige and Skoog solid medium coupled with growth regulator substances with respective concentrations of 0; 0.5; 1; 1.5; 2 mg / L. The data obtained in the form of quantitative and qualitative data. Quantitative data include duration of callus induction, percentage of callus form explants, fresh callus weight and dry weight of callus, analyzed statistically with SPSS. Qualitative data include callus color and texture. The results showed that NAA and BAP growth regulator effect on growth of P. retrofractum leaf eksplan. The addition of a combination of NAA concentration of 0.5 mg / L and BAP 0.5 mg / L showed the fastest callus formation response of 11.5 days. The combination of NAA concentration of 1 mg / L and BAP 0,5 mg / L resulted in the best fresh weight of 70.6 mg, while in combination NAA concentration 1 mg / L and BAP 2 mg / L yielded the best dry weight of 18 mg. Callus color is white and white  with a friable and compact texture.Keywords:BAP,   callus     induction,             NAA,       Piper      retrofractum         Vahl

scholarly journals Peranan Zat Pengatur Tumbuh dalam Perbanyakan Tanaman melalui Kultur Jaringan

2011 ◽  
Vol 7 (1) ◽  
pp. 63 ◽  
Author(s):  
Endang Gati Lestari
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Plant Growth ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Plant Tissue ◽  
Callus Production ◽  
Control Root ◽  
Other Substances

<p>The Role of Growth Regulator in Tissue Culture Plant<br />Propagation. Endang G. Lestari. In plant tissue culture,<br />growth regulator has significant roles such as to control root<br />and shoot development in the plant formation and callus<br />induction. Cytokinin and auxin are two prominent growth<br />regulator. Cytokinin consists of BA (benzil adenin), kinetin<br />(furfuril amino purin), 2-Ip (dimethyl allyl amino purin), and<br />zeatin. While auksin covers IAA (indone acetic acid), NAA<br />(napthalene acetic acid), IBA (indole butiric acid) 2.4-D (2.4-<br />dicholophenoxy acetic acid), dicamba (3,6 dicloro-O-anisic<br />acid), and picloram (4-amino 3,5,6-tricloropicolinic acid).<br />The emphasis of plant growth purposes decide the use of<br />growth regulator. Cytokinin is applied mainly for the purpose<br />of shoot, while auxin is mainly used for the purpose of root<br />and callus. The application of growth regulator application is<br />varied, depending on the genotype and physiological<br />condition of the plant. The existence of a certain growth<br />regulating substances can enhance growth regulator activity<br />of other substances. The type and concentration of the<br />appropriate growth regulators for each plant is not the same<br />because it depends on the genotype and physiological<br />condition of plant tissue. However so often both are<br />frequently required depend on the ratio/ratio of auxin<br />cytokines or vice versa. The existence of a certain growth<br />regulating substances can enhance growth regulator activity<br />of other substances. The type and concentration of the<br />appropriate growth regulators for each plant is not the same<br />because it depends on the genotype and physiological<br />condition of plant tissue. For the propagation, multiple and<br />adventive shoots along with embriosomatic formation could<br />be applied. The seedling is obtained from one somatic cell.<br />Here, strong auxin, such as dicamba and picloram 2.4-D, is<br />utilized for callus production. For this reason, seedling per<br />unit could be produced more than that of organogenesis.</p>

Callus Induction and Plant Regeneration in Rauvolfia Serpentina (L.) Benth Ex. Kurz.

2009 ◽  
Vol 24 ◽  
pp. 82-88 ◽  
Author(s):  
Saraswoti Aryal ◽  
Sanu Devi Joshi
Keyword(s):  
Acetic Acid ◽  
Callus Induction ◽  
Coconut Milk ◽  
Ms Medium ◽  
Rauvolfia Serpentina ◽  
History Museum ◽  
Short Period ◽  
Murashige Skoog ◽  
Callus Tissue Culture

Rauvolfia serpentina (L.) ex. Kurz is an important medicinal plant. Callus induction and regeneration was studied from stem explant of in-vitro grown plant of Rauvolfia serpentina(L.) Benth. ex Kurz (Apocynaceae) on Murashige Skoog (1962) medium supplemented with 1mg/l 2,4-Dichlorophenocy acetic acid (2,4-D) and 1mg/l Kinetin (Kn). Vigorous growth of callus occurs after 4 weeks of culture. Callus was sub-cultured on Murashige and Skoog (MS) medium supplemented with different concentration of 2, 4-D (0.5-3.0 mg/l) and 10% coconut milk. Regeneration of plantlets occurred on MS medium containing 3 mg/1 of 2, 4-D and 10% coconut milk. These plantlets were rooted on MS medium supplemented with 1 mg/l IAA .The regenerated plantlets were able to grow on soil after short period ofacclimatization. Key words: Explant; In-vitro culture; MS medium;  2, 4 Dichlorophenoxy acetic acid; Kinetin; Callus; Tissue culture; Coconut milk. Journal of Natural History Museum Vol. 24, 2009 Page: 82-88

scholarly journals In vitro Regeneration of Dalbergia sissoo Roxb. and the Potential for Genetic Transformation

2012 ◽  
Vol 40 (2) ◽  
pp. 140 ◽  
Author(s):  
Hafiz Mamoon REHMAN ◽  
Iqrar Ahmad RANA ◽  
Siddra IJAZ ◽  
Ghulam MUSTAFA ◽  
Faiz Ahmad JOYIA ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Genetic Transformation ◽  
Callus Induction ◽  
In Vitro Regeneration ◽  
Induction Medium ◽  
Native Forest ◽  
Ms Medium ◽  
Dalbergia Sissoo ◽  
Callus Induction Medium

Dalbergia sissoo Roxb. ex DC. (Sissoo) is a native forest tree species in Pakistan. Many ecological and economical uses are associated with this premier timber species, but dieback disease is of major concern. The objective of this study was to develop a protocol for in vitro regeneration of Sissoo that could serve as target material for genetic transformation, in order to improve this species. Callus formation and plantlet regeneration was achieved by culturing cotyledons, immature seeds, and mature embryos on a modified Murashige and Skoog (1962) (MS) medium supplemented with plant growth regulators. Callus induction medium containing 2.71 ?M 2, 4-dichlorophenoxyacetic acid (2,4-D) and 0.93 ?M kinetin produced better callus on all explants tested compared to other treatments, such as 8.88 ?M 6-benzylaminopurine (BA) and 2.69 ?M ?-naphthalene acetic acid (NAA), or 2.71 ?M 2, 4-D and 2.69 ?M NAA. Shoot regeneration was best on MS medium containing 1.4 ?M NAA and 8.88 ?M BA compared to other treatments, such as 1.4 ?M NAA and 9.9 ?M kinetin, or 2.86 ?M indole-3-acetic acid and 8.88 ?M BA. Murashige and Skoog medium containing 1.4 NAA ?M and 8.88 ?M BA was better in general for regeneration regardless of callus induction medium and the type of explant used. Rooting was best on half-strength MS medium with 7.35 ?M indole-3-butyric acid. Regenerated plantlets were acclimatized for plantation in the field. Preliminary genetic transformation potential of D. sissoo was evaluated by particle bombardment of callus explants with a pUbiGus vector. The bombarded tissue showed transient Gus activity 1week after bombardment. Transformation of this woody tree is possible provided excellent regeneration protocols. The best combination for regeneration explained in this study is one of such protocols.

scholarly journals Role of Growth Regulators on In Vitro Callus Induction and Direct Regeneration in Physalis minima Linn

2015 ◽  
Vol 44 ◽  
pp. 38-44 ◽  
Author(s):  
H. Sandhya ◽  
Rao Srinath
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Callus Induction ◽  
Basal Medium ◽  
Direct Regeneration ◽  
Naphthalene Acetic Acid ◽  
Stem Explants ◽  
Dichlorophenoxy Acetic Acid ◽  
Indole 3 Acetic Acid

Suitable protocol for induction of callus and regeneration was developed from different explants viz., node, stem and leaves in Physalis minima. MS basal medium supplemented with various concentrations (1.0-4.0mg/l) of auxins like 2,4-Dichlorophenoxy acetic acid (2,4-D), α-naphthalene acetic acid (NAA) and Indole-3-acetic acid (IAA) and cytokinins (0.5-1.5mg/l) like BAP or Kn were used. All the three explants responded for induction of callus, however stem explants were found superior, followed by node and leaf. Callus induction was observed in all the auxins and combination of growth regulators used with varied mass (2010±1.10) and highest percentage of callus induction was observed from stem at 2.0mg/l 2,4-D (90%) followed by NAA (70%) and IAA (50%). Organogenesis was induced when nodal explants were transferred on MS medium supplemented with 2,4-D and Kn at various concentrations, maximum being on 2.0mg/l 2,4-D + 1.0mg/l Kn (90%). Regenerated shoots were elongated on 0.5mg/l GA3. The shoots were subsequently rooted on MS + 1.0mg/l IBA (95%) medium. Rooted shoots were hardened and acclimatized, later they were transferred to polycups containing soil, cocopeat and sand in the ratio 1:2:1.Keywords:Physalis minima, Node, Stem, Leaf, callus and growth regulators.

scholarly journals Effects of Plant Growth Regulators and Sugars on Ehretia asperula Zoll. et Mor. Cell Cultures

2021 ◽  
Author(s):  
P.T.M. Tram ◽  
N.K. Suong ◽  
L.T.T. Tien
Keyword(s):  
Cell Suspension ◽  
Callus Induction ◽  
Malignant Tumors ◽  
Single Cells ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Dichlorophenoxyacetic Acid ◽  
Human Immune System ◽  
Friable Callus ◽  
2,4 Dichlorophenoxyacetic Acid

Background: Belonging to the Boraginacae family, Ehretia asperula Zoll. et Mor., called “Xa den”, is a precious medicinal plant also known as the “cancer tree” by the Muong ethnic group in Hoa Binh Province, Vietnam. Xa den has been demonstrated to inhibit the development of malignant tumors, reduce oxidation and enhance the human immune system. This research focused on examining friable callus induction from young stems of Ehretia asperula Zoll. et Mor. Methods: Samples of Xa den were less than two years old. Young stems with 2 to 6 leaves served as explants for callus induction. Explants placed on autoclaved B5 nutrients incubated at 25oC, in the dark. The testing factors were concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) and Benzyl adenine (BA), types and concentrations of sugars.Result: Friable callus was induced on B5 medium with 0.4 mg/L of 2,4-D, 0.1 mg/L of BA and 30 g/L of glucose at the highest rate (100%). Additionally, callus grew best after 5 weeks of culture weighing 0.194 g. Friable callus was used as material for cell suspension cultures. After two weeks, the Xa den cell suspension cultures contained single cells and small cell clumps. The liquid medium had changed from dark yellow to light brown.

scholarly journals Organogenesis Kelapa Sawit (Elaeis guineensis Jacq.) Asal Eksplan Bunga Betina

2016 ◽  
Vol 2 (2) ◽  
pp. 104
Author(s):  
Tyas Larasati ◽  
Suci Rahayu ◽  
Fauziyah Harahap
Keyword(s):  
Plant Growth ◽  
Growth Regulator ◽  
Callus Induction ◽  
Plant Growth Regulator ◽  
Elaeis Guineensis ◽  
Female Flower ◽  
Dry Weight ◽  
Flower Plant ◽  
Elaeis Guineensis Jacq ◽  
Dichlorophenoxy Acetic Acid

The objectives of this research were to composed organ from callus culture and to found the best concentration of plant growth regulator for organ growth from female flower explant of oil palm. This research has already done from June 2014 to May 2015 at Laboratory of Plant Physiology and Tissue Culture Department of Biology Faculty of Mathematics and Science University of North Sumatera. This research used Nonfactorial Completely Random Design. Explant was treated with five concentrations of 2,4-Dichlorophenoxy acetic acid (2,4-D; 99, 110, 120, 132, and 140 mg/L) for callus induction on Y3 medium (Eeuwens 1976). The result of this research showed that organ was formed from this treatment (basal segment of female flower explant) was root organ. 2,4-D plant growth regulator positively affected to growing of the root. The best result for time of callus induction, time of root growth, the highest percentage of explants that formed the root, fresh weight and dry weight of callus that has become the root generation was resulted from 99 mg/L 2,4-D.   Key words: Elaeis guineensis Jacq., female flower, plant growth regulator 2,4-D, organogenesis

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