Effectiveness of Clove Nano Biopesticides Against Mosaic Virus in Patchouli

Clove oil has the potential to suppress the development of the mosaic virus in patchouli plants, but its effectiveness in the field has not been studied. This study aimed to evaluate the effect of clove nano biopesticide on controlling patchouli mosaic disease. The research was conducted at the Manoko Experimental Garden, Bandung, West Java from March to November 2018. The patchouli used was Patchoulina-2 variety, which originated from the Seed Breeder Garden in Lembang, Bandung. This study was arranged in a Randomized Block Design (RBD), consisting of five treatments and ten replications within each treatment, with one hundred plants in each replication. The results obtained showed that nano biopesticides of citronella, clove, and commercial citronella (Asimbo) were able to reduce the incidence and intensity of mosaic diseases in patchouli plants, showing the efficacy levels of 14.68%, 9.06%, and 5.83%, respectively. The application of citronella and clove biopesticides on Patchoulina-2 every month could increase plant fresh weight, when compared to the plants without treatment. Patchoulina-2 plants treated with nano biopesticides of clove and commercial citronella (Asimbo) showed higher value of fresh weight compared to those treated with citronella nano biopesticide. The clove nano biopesticide can also be developed to control mosaic diseases in patchouli plants.

The Occupancy of Barn Owl in the Artificial Nest Box to Control Rice Field Rat in Yogyakarta Indonesia

One component of integrated rat management in rice fields is barn owl as biological control. The study was conducted to evaluate the occupancy rate of barn owl nest boxes, the local rat population, and rat damage to rice crops. It was conducted in three locations in Yogyakarta by observing 10 nest boxes per site. Assessment of their occupancy was monitored by barn owl presence in nest box (egg, chick, and adult) and natural nests in villages nearby. In comparison with control village, the local rat population was observed by the active burrow count method and linear trap barrier systems. Rat damage intensity is estimated by sampling 150 tillers using a stratified sampling approach. The result indicated that 1-4 nest boxes were occupied per location. The owls also nested within buildings nearby. Active burrows ranged from 4 to 25 burrows per 150 m. The rat damage area ranged from 33.33% - 48.57% with 6.33% - 14.86% damage intensity was significantly lower than the control site. Artificial nest box installation for owls in rice fields were only occupied for breeding. The use of barn owls for biological control of rice field rats should be combined with other methods in an integrated approach.

Response of Corn-Soybean Intercropping to Fertilizer Packages in Dry Land with Dry Climate

Intercropping soybean with corn on dry land with dry climate (DLDC) is an alternative program to expand the soybean cultivation harvested area. This study evaluated the effectiveness of fertilization performance in the intercropping of soybean-corn in DLDC. The experiment in this study was arranged in a randomized block design, consisting of seven fertilizer package treatments with four replications. The spacing between corn (Pertiwi 3) and soybean (Dena 1) was (50 cm x 200 cm) x 40 cm (2 plants/clump) and between soybeans (Dena 1) was 40 cm x 15 cm (2-3 plants/clumps). The observations consisted of soil analysis (pH, organic matter, total N (Kjeldahl), available P, Ca-dd, Mg-dd, K-dd, and Na-dd), soybean leaf chlorophyll index (45 and 60 days after planting/dap), plant height at (45 daps and harvest), number and weight of root nodules (45 daps); Corn: chlorophyll index (56 daps), plant height (harvest), analysis of corn and soybean plant tissue (60 daps), yield, and yield components of dry seeds of soybean and corn per hectare. The results showed that effective fertilization for the intercropped crops was 53 kg N + 1,500 kg of manure per hectare in corn plant and 7 kg N + 22 kg P2O5 + 18 kg K2O + 1,500 kg/ha manure + Rhizobium Iletrisoy/Agrisoy in soybean crops.

Response of Growth and Yield of Soybean (Glycine max l. Merril) to the Method and Dose of Leachate Liquid Organic Fertilizer Application

The high demand for soybeans cannot yet be met by domestic production. Thus, a way to increase soybean production is necessary, one of which is to use liquid organic fertilizer derived from landfill leachate. This study aimed to examine the application method of liquid organic fertilizer (LOF) from landfill leachate to the soil and leaves and to determine the concentration giving the best growth and yield of soybean plants. The study was a field-research arranged in a completely randomized design (CRD) consisting of six treatments and four replications, which was carried out in a greenhouse. The treatments include A (0 LOF/L water), B (20 ml LOF/L water), C (30 ml LOF/L water, D (40 ml LOF/L water), E (50 ml LOF/L water) and F (60 ml LOF/L water). The liquid organic fertilizer was applied through the soil and leaves at a dose according to the treatments. The results showed that the application of liquid organic fertilizer from landfill leachate was better applied to the soil than to the leaves. The liquid organic fertilizer given had a significant effect on the leaf area (cm2), crop weight (g), and plant seed weight (g), but not on the plant height (cm) and stem diameter (cm). Liquid organic fertilizer application is better given to the soil than to the leaves based on the independent T test. The liquid organic fertilizer from landfill leachate at a concentration of 40 ml/L of water had the best effect on the growth and yield of soybean plants.

The Potential of Biogas Slurry and Palm Oil Mill Effluent Slurry as Slow-Release Fertilizer Pellet Through Densification

Organic fertilizer can yield higher production compared to regular fertilizer if properly applied. Thus, it can be a solution to improve nutrient content of soil. The biggest source of bio slurry in plantation is from Palm Oil Mill Effluent (POME) and cow dung biogas. This research aimed to analyze the residue’s potential from the result of biogas processing and bio slurry from POME as slow-release fertilizer pellet. Bio slurry was processed into pellet through densification process using pellet mill. The research was arranged in a Randomized Block Design method with five slurry compositions as treatments, including 70:30, 60:40, 50:50, 40:60, and 30:70 (ratio of biogas slurry and POME slurry), each consisted of three replications. According to the data obtained, fertilizer pellets had characteristics of 25 – 29 mm of length, 5.23 – 5.85 mm of diameter, 0.44 – 0.53 g/cm3 of density, 54.78% - 81.96% of durability, and 7.81% - 8.57% of moisture content. Based on density and durability aspects, 30:70 composition was the higher. Macronutrient content of the five compositions were 1.88% - 2.72%, in which on day 22, N, P, and K release was 0.36 – 1.01%, 73.51 – 97.48%, and 3.19 – 7.85%, respectively. Meanwhile, on day 17, the nutrition solution conductivity of all compositions had already reached 0.80 – 1 mS/cm.

Exploration of Arbuscular Mycorrhizal Fungi from Sugarcane Rhizosphere in Marginal Land

The exploration of arbuscular mycorrhiza fungi from sugarcane plantation in marginal land in South Sulawesi was carried out to find the source of inoculums showing effective infection. Soil samples were taken from four area with different characteristic of marginal land, namely land with low organic matter content, clay texture, limited irrigation, and undulating land. Mycorrhizae contained in the soil samples were then observed, and the spores obtained were used as the source of isolation by a single spore culture. The mycorrhizal spores were isolated by wet sieving and centrifugation method with 48% sucrose, which were observed under a compound microscope for spore details (100-1000x). Sugarcane root samples were taken to observe mycorrhizal infection in sugarcane root tissue by root staining method. The results of the study showed that the greatest diversity of mycorrhizal genera was found in soil samples of Jambua Block (Glomus, Gigaspora, and Sclerocistis) and AJ-5 Block area (Glomus, Acaulospora, and Sclerocistis). Single-spore isolates obtained were Glomus sp. and Acaulospora sp. Infection test result on four sugarcane varieties commonly grown in Takalar Sugar Factory showed that infectivity of mycorrhizal isolate of Acaulospora sp. was the highest (75%) and significantly different (LSD’test, p 0,05) compared to that of Glomus sp. (66%).

Sterilisasi dan Induksi Daun Muda Durian (Durio zibethinus) Dalam Medium MS Dengan Penambahan Kinetin dan IAA Secara In Vitro

A research to study the sterilization method and application of Kinetin and IAA to induce the Durian young leaf (Durio zibethinus) in MS medium was conducted in Balai Benih Induk Hortikultura in Salaman Magelang district of Central Java started on September until December 2003. The Laboratory experiment was arranged in two phases, which were the optimation phase of sterilization and induction phase. At the first phase, the sterilization method used was the modification of Mulya (2001) method. The modification use of sterilant, vitamin C antioxidant, Alcohol 70 %, Benlate, Agrept, Tween-20 and Betadine were done to obtain effectiveness of the sterilization. Explants planted then in MS medium for two weeks. Contamination time, percentage of contamination and viabilitas (percentage of living explants) were observed then. At the second phase, the treatments were arranged in a 3 x 3 factorial completely randomized design (CRD) to observed the influence of Kinetin and IAA combination. The concentration of Kinetin observed were 2, 4, and 6 mg/I, where as the IAA concentration were 0.5, 1.0, and 1.5 mg/I. All treatments were repeated three times, with three samples on each replication. The percentage of browning explants, percentage of contaminated explants, site of contamination and percentage of explants live were observed at the end of incubation. The results showed that sterilization of Durian young leaves explants with 1 g/l deterjent for 15 minutes then by 2 g/l Benlate and Agrept for 10 minutes, then by 1 g/200 mg Vitamin C, then by Alcohol 70 % for 1 minute, then by 20% Clorox, then by 2 drip of Tween-20 for 10 minute and then by Betadine decreased the contamination down to 50 %, and this kind of sterilization was relatively better than the other kinds. Application of growth regulators were not able to induce explants growth, but stimulated callus formation at the cutting surface though, in the application of Kinetin 4 mg/1 + IAA 0,5 mg/I, Kinetin 4 mg/1 + IAA 1,5 mg/1, Kinetin 6 mg/I+ IAA 0,5 mg/1 and Kinetin 6 mg/l+IAA 1,0 mg/I.

Pengaruh Pemberian Zeolite dan Dosis Pupuk Nitrogen Terhadap Pertumbuhan Vegetatif Jagung (Zea mays, L.) di Media Pasir Pantai

A research to study the effects of Zeolite and nitrogen fertilizer application on vegetative growth of maize crop in coastal sandy soil was conducted on the research field of Agriculture Faculty, Muhammadiyah University of Yogyakarta at Tamantirto, Bantul District of Yogyakarta during October until February, 2003. This research was aimed on the study of Zeolite application in order to enhance the nitrogen uptake in the coastal sandy soil, and its effects on vegetative growth of CP I variety of maize. The pot experiment was arranged in 7 x 3 factorial completely randomized design, with 4 replications. The first factor was Zeolite dosage consisted of 7 levels, i.e: 0, 3, 4, 5, 6, 7, and 8 %; and the second one was 3 levels nitrogen fertilizer dosage which consisted of: 75, 110, and 145 kg/ ha. The nitrogen fertilizer was applied on the medium mixed with Zeolite granules. Observations on plant height, stem diemeter, leaves number, plant fresh and dry weight were done during the vegetative growth of maize. The results showed that Zeolite application significantly enhanced the vegetative growth of CPI maize. The 5,2 - 5,6 % Zeolite was optimum to gave the maximum growth of maize crop. Nitrogen fertilizer application was also significantly increased the plant height and biomass weight, and the 145 kg/ha was the optimum dosage. There was no interaction between Zeolite and nitrogen fertilizer application in order to affected the maize growth.

Kepadatan Populasi Wereng Coklat (Nilaparvata lugens, Stal.) dan Musuh Alaminya Pada Berbagai Varietas Padi di Bantul Yogyakarta

A research to observe the population density of brown planthoppers (Nilaparvata lugens, Stal.) and their predator and parasitoid natural enemies has done on the rice field in Bantul regency of Yogyakarta. The research was conducted to observe the hoppers and its natural enemies on three local varieties and one superior brown planthoppers resistant variety of rice. The three local varieties of rice were Rejosari, Sri Kuning, and Sri Ayu, while the hopper resistant variety was IR 64. The observation were replicated three times. The result showed that population of brown planthoppers in the three local varieties of rice were higher than in the resistant IR 64 hybrid variety. The population of predators and parasitoid were relatively high in order to control the development of brown planthopper population. The predator Lycosa sp. and the parasitoids Anagrus sp. were commonly found in the ecosystem.

Pengaruh Berbagai Macam Pendinginan dan Pengemasan Terhadap Umur Simpan Jagung Manis (Zea mays saccharata)

This research was aimed to study the influence of kinds of cooling and packing on the storage life of sweetcorn in order to obtain the simple postharvest technology and easy to practice. The research was done during the February - March of 2005 in the Research Laboratory of Agriculture Faculty, Muhammadiyah University of Yogyakarta. The reducing sugar analysis was done in the Agriculture Technology Faculty of Gadjah Mada University. The laboratory experiment was arranged ini 2 x 3 factorial completely randomized design with three replications. The cooling first factor was refrigerated in refrigerator to be compared to the hydro-cooling. Meanwhile, the polypropilene and polyethylene packaging were tested and compared to the unpackaged one. The flavor; reducing sugar, water content, percentage of nonconsumable part of sweetcorn, fresh weight of ears, and organoleptic properties were observed. The result showed that there was no significantly interaction between cooling and packaging on the storage life and quality of sweet corn. The cooling treatment was significantly influenced the starch and reducing sugar level as well as the packaging treatment. Refrigeration storage could significantly keep the level of reducing sugar to remain higher than the hydro-cooling, this treatment was also decreased the starch content, decreased the proportion of unconsumable part, and maintained the flavor. The polyethylene packaging significantly keep the level of reducing sugar to remain higher and relatively maintained the flavor than the polypropilene packaging or unpackaged, but decreased the portion of unconsumable part.