Features of solubility and cytocompatibility in vitro of bone cements based on calcium sulfate containing phosphate ions

2021 ◽  
pp. 39-48
Author(s):  
D.R. Khairutdinova ◽  
◽  
M.A. Goldberg ◽  
P.A. Krokhicheva ◽  
O.S. Antonova ◽  
...  
Keyword(s):  
Calcium Sulfate ◽  
Human Blood Plasma ◽  
Bone Cements ◽  
Phosphate Ions ◽  
Calcium Phosphate Layer ◽  
Matrix Properties ◽  
Sbf Solution ◽  
Phosphate Buffered Saline ◽  
Phosphate Groups

Cement materials have based on calcium sulfate were obtained, which have introduced up to 20 mol. % of phosphate groups to improve their cytocompatibility and solubility. It was found that during exposure of cement materials based on calcium sulfate, containing of 0, 5, 10 and 20 mol. % phosphate - groups, in model solutions SBF (Kokubo solution, simulating the composition of human blood plasma) and DPBS (Dulbecco's phosphate-buffered saline) in dynamics up to 28 days of were formation of a calcium-phosphate layer on the surface of the samples. At the same time, in SBF solution, the process of recrystallization of sulfate and phosphate phases occurs more pronounced than in DPBS solution. In vitro studies on the cell culture of the human osteosarcoma line MG-63, cement samples showed cytocompatibility with the presence of moderate surface matrix properties

scholarly journals Comparison of Borate Bioactive Glass and Calcium Sulfate as Implants for the Local Delivery of Teicoplanin in the Treatment of Methicillin-Resistant Staphylococcus aureus-Induced Osteomyelitis in a Rabbit Model

2015 ◽  
Vol 59 (12) ◽  
pp. 7571-7580 ◽  
Author(s):  
Wei-Tao Jia ◽  
Qiang Fu ◽  
Wen-Hai Huang ◽  
Chang-Qing Zhang ◽  
Mohamed N. Rahaman
Keyword(s):  
Staphylococcus Aureus ◽  
Bioactive Glass ◽  
Calcium Sulfate ◽  
Rabbit Model ◽  
Local Delivery ◽  
Methicillin Resistant ◽  
Content Type ◽  
Borate Bioactive Glass ◽  
Phosphate Buffered Saline

ABSTRACTThere is growing interest in biomaterials that can cure bone infection and also regenerate bone. In this study, two groups of implants composed of 10% (wt/wt) teicoplanin (TEC)-loaded borate bioactive glass (designated TBG) or calcium sulfate (TCS) were created and evaluated for their ability to release TECin vitroand to cure methicillin-resistantStaphylococcus aureus(MRSA)-induced osteomyelitis in a rabbit model. When immersed in phosphate-buffered saline (PBS), both groups of implants provided a sustained release of TEC at a therapeutic level for up to 3 to 4 weeks while they were gradually degraded and converted to hydroxyapatite. The TBG implants showed a longer duration of TEC release and better retention of strength as a function of immersion time in PBS. Infected rabbit tibiae were treated by debridement, followed by implantation of TBG or TCS pellets or intravenous injection with TEC, or were left untreated. Evaluation at 6 weeks postimplantation showed that the animals implanted with TBG or TCS pellets had significantly lower radiological and histological scores, lower rates of MRSA-positive cultures, and lower bacterial loads than those preoperatively and those of animals treated intravenously. The level of bone regeneration was also higher in the defects treated with the TBG pellets. The results showed that local TEC delivery was more effective than intravenous administration for the treatment of MRSA-induced osteomyelitis. Borate glass has the advantages of better mechanical strength, more desirable kinetics of release of TEC, and a higher osteogenic capacity and thus could be an effective alternative to calcium sulfate for local delivery of TEC.

scholarly journals Degradation and In Vivo Response of Hydroxyapatite-Coated Mg Alloy

Coatings ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 375 ◽  
Author(s):  
Yevheniia Husak ◽  
Oleksandr Solodovnyk ◽  
Anna Yanovska ◽  
Yevhenii Kozik ◽  
Iryna Liubchak ◽  
...  
Keyword(s):  
Surface Morphology ◽  
Fibrous Tissue ◽  
Human Blood Plasma ◽  
Citrate Buffer ◽  
Physiological Conditions ◽  
Hydroxyapatite Coatings ◽  
Sbf Solution ◽  
New Generation

Nowadays there is a need for new generation of biodegradable implants, which should be able to stimulate the healing responses of injured tissues at the molecular level. Magnesium alloys attract great attention as perspective bone implants due to their biocompatibility, physical properties and ability to degrade completely under physiological conditions. The main purpose of this research was assessment of in vitro corrosion and surface morphology after short term in vivo implantation of Mg based implant covered by hydroxyapatite (HA). Mg alloys with the addition of Zr (0.65%), Al (1.85%) and Nd (1.25%) were used. In our work, we propose dipping method for hydroxyapatite coatings formation which has been shown to reduce the corrosion rate of magnesium implants in vivo. Simulated body fluid (SBF; pH 7.4) with ion concentrations approximately equal to those of human blood plasma resembling physiological conditions and citrate buffer with pH 5—simulating inflammation were selected as modelling environments for in vitro degradation test. The rod samples were implanted into the tibia bone of rats and after 1 day and 5 days of implantation were taken out to observe cells adhesion on surface samples. SEM was used to assess surface morphology after in vitro and in vivo tests. SBF solution causes some cracks on the surface of HA coatings, while citrate solution at pH 2 caused complete dissolving of the coating. The HA coating favoured cell adhesion and rapid fibrous tissue formation.

The Effect of Phosphate Groups on the Structure and Properties of Bone Cements Based on Calcium Sulfate

2020 ◽  
Vol 493 (2) ◽  
pp. 117-120
Author(s):  
D. R. Khayrutdinova ◽  
O. S. Antonova ◽  
M. A. Golgberg ◽  
S. V. Smirnov ◽  
P. A. Krokhicheva ◽  
...  
Keyword(s):  
Calcium Sulfate ◽  
Bone Cements ◽  
Structure And Properties ◽  
Phosphate Groups

scholarly journals In vitro degradability, bioactivity and primary cell responses to bone cements containing mesoporous magnesium–calcium silicate and calcium sulfate for bone regeneration

2015 ◽  
Vol 12 (111) ◽  
pp. 20150779 ◽  
Author(s):  
Yueting Ding ◽  
Songchao Tang ◽  
Baoqing Yu ◽  
Yonggang Yan ◽  
Hong Li ◽  
...  
Keyword(s):  
Vitamin D ◽  
Bone Regeneration ◽  
Calcium Silicate ◽  
Calcium Sulfate ◽  
Degradation Products ◽  
Setting Time ◽  
Bone Cements ◽  
Release Ratio ◽  
Acidic Degradation

Mesoporous calcium sulfate-based bone cements (m-CSBC) were prepared by introducing mesoporous magnesium–calcium silicate (m-MCS) with specific surface area (410.9 m² g −1 ) and pore volume (0.8 cm³ g −1 ) into calcium sulfate hemihydrate (CSH). The setting time of the m-CSBC was longer with the increase of m-MCS content while compressive strength decreased. The degradation ratio of m-CSBC increased from 48.6 w% to 63.5 w% with an increase of m-MCS content after soaking in Tris–HCl solution for 84 days. Moreover, the m-CSBC containing m-MCS showed the ability to neutralize the acidic degradation products of calcium sulfate and prevent the pH from dropping. The apatite could be induced on m-CSBC surfaces after soaking in SBF for 7 days, indicating good bioactivity. The effects of the m-CSBC on vitamin D 3 sustained release behaviours were investigated. It was found that the cumulative release ratio of vitamin D 3 from the m-CSBC significantly increased with the increase of m-MCS content after soaking in PBS (pH = 7.4) for 25 days. The m-CSBC markedly improved the cell-positive responses, including the attachment, proliferation and differentiation of MC3T3-E1 cells, suggesting good cytocompatibility. Briefly, m-CSBC with good bioactivity, degradability and cytocompatibility might be an excellent biocement for bone regeneration.

scholarly journals Formation and Evolution of Nanoscale Calcium Phosphate Precursors Under Biomimetic Conditions

2021 ◽  
Author(s):  
ludovica epasto ◽  
tristan georges ◽  
albina selimovic ◽  
Jean-Michel Guigner ◽  
Thierry Azaïs ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Building Blocks ◽  
Human Blood Plasma ◽  
Spherical Building ◽  
Ionic Clusters ◽  
Research Fields ◽  
Phosphate Ions ◽  
Formation And Evolution ◽  
Conformational Rearrangements

Simulated body fluids that mimic human blood plasma are widespread media for in-vitro studies in an extensive array of research fields, from biomineralization to surface and corrosion sciences. We show that these solutions undergo dynamic nanoscopic conformational rearrangements on the timescale of minutes to hours, even though they are commonly considered stable or metastable. In particular, we find and characterize nanoscale inhomogeneities made of calcium phosphate (CaP) aggregates that emerge from homogeneous SBF within a few hours and evolve into prenucleation species (PNS) that act as precursors in CaP crystallization processes. These ionic clusters consist of about 2 nm large spherical building units that can aggregate into supra-structures with sizes of over 200 nm. We show that the residence times of phosphate ions in the PNS depend critically on the total PNS surface. These findings are particularly relevant for understanding non-classical crystallization phenomena, in which PNS are assumed to act as building blocks for the final crystal structure.<br>

scholarly journals In-vitro bioactivity evaluation of titanium layers manufactured on implants with LENS method

Mechanik ◽  
2017 ◽  
Vol 90 (1) ◽  
pp. 48-49
Author(s):  
Bartłomiej Wysocki ◽  
Agata Supeł ◽  
Tomasz Durejko ◽  
Wojciech Święszkowski
Keyword(s):  
Body Fluid ◽  
Hip Prosthesis ◽  
Calcium Phosphates ◽  
Human Blood Plasma ◽  
In Vitro Bioactivity ◽  
Laser Engineering ◽  
Sbf Solution ◽  
Net Shaping ◽  
Scanning Electron

Laser Engineering Net Shaping (LENS), which is one of the 3DP techniques, allows for both, the fabrication and the modification of surface layer of the product previously prepared. In the current work, using LENS technique, highly developed bioactive layers, were prepared on the surface of the hip prosthesis. Both, fabricated layers and hip prosthesis, were fabricated from Ti-6Al-4V alloy. The tests were carried out using Simulated Body Fluid (SBF) solution, having all the necessary components on the inorganic human blood plasma. Scanning Electron Microscopy (SEM) showed the nucleation of calcium phosphates even after 2 days of immersion. Based on the results obtained in SEM and EDX tests, it can be claimed that Ti-6Al-4V layers manufactured using LENS technique exhibit high bioactivity in in-vitro, in the SBF solution.

scholarly journals Formation and Evolution of Nanoscale Calcium Phosphate Precursors Under Biomimetic Conditions

2021 ◽  
Author(s):  
ludovica epasto ◽  
tristan georges ◽  
albina selimovic ◽  
Jean-Michel Guigner ◽  
Thierry Azaïs ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Building Blocks ◽  
Human Blood Plasma ◽  
Spherical Building ◽  
Ionic Clusters ◽  
Research Fields ◽  
Phosphate Ions ◽  
Formation And Evolution ◽  
Conformational Rearrangements

Simulated body fluids that mimic human blood plasma are widespread media for in-vitro studies in an extensive array of research fields, from biomineralization to surface and corrosion sciences. We show that these solutions undergo dynamic nanoscopic conformational rearrangements on the timescale of minutes to hours, even though they are commonly considered stable or metastable. In particular, we find and characterize nanoscale inhomogeneities made of calcium phosphate (CaP) aggregates that emerge from homogeneous SBF within a few hours and evolve into prenucleation species (PNS) that act as precursors in CaP crystallization processes. These ionic clusters consist of about 2 nm large spherical building units that can aggregate into supra-structures with sizes of over 200 nm. We show that the residence times of phosphate ions in the PNS depend critically on the total PNS surface. These findings are particularly relevant for understanding non-classical crystallization phenomena, in which PNS are assumed to act as building blocks for the final crystal structure.<br>

scholarly journals AnIn VitroComparison of PMMA and Calcium Sulfate as Carriers for the Local Delivery of Gallium(III) Nitrate to Staphylococcal Infected Surgical Sites

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Rebecca A. Garcia ◽  
David J. Tennent ◽  
David Chang ◽  
Joseph C. Wenke ◽  
Carlos J. Sanchez
Keyword(s):  
Antimicrobial Activity ◽  
Calcium Sulfate ◽  
Bacterial Colonization ◽  
Release Kinetics ◽  
Bacterial Biofilms ◽  
Bone Cements ◽  
Planktonic Bacteria ◽  
Orthopaedic Infections ◽  
Potential Use

Antibiotic-loaded bone cements, including poly(methyl methacrylate) (PMMA) and calcium sulfate (CaSO4), are often used for treatment of orthopaedic infections involvingStaphylococcusspp., although the effectiveness of this treatment modality may be limited due to the emergence of antimicrobial resistance and/or the development of biofilms within surgical sites. Gallium(III) is an iron analog capable of inhibiting essential iron-dependent pathways, exerting broad antimicrobial activity against multiple microorganisms, includingStaphylococcusspp. Herein, we evaluated PMMA and CaSO4as carriers for delivery of gallium(III) nitrate (Ga(NO3)3) to infected surgical sites by assessing the release kinetics subsequent to incorporation and antimicrobial activity againstS. aureusandS. epidermidis. PMMA and to a lesser extent CaSO4were observed to be compatible as carriers for Ga(NO3)3, eluting concentrations with antimicrobial activity against planktonic bacteria, inhibiting bacterial growth, and preventing bacterial colonization of beads, and effective against established bacterial biofilms ofS. aureusandS. epidermidis. Collectively, ourin vitroresults indicate that PMMA is a more suitable carrier compared to CaSO4for delivery of Ga(NO3)3; moreover they provide evidence for the potential use of Ga(NO3)3with PMMA as a strategy for the prevention and/or treatment for orthopaedic infections.

In vitro Behaviour of Alumina-Hydroxiapatite Composites Coatings

2018 ◽  
Vol 69 (6) ◽  
pp. 1416-1418
Author(s):  
Alexandru Szabo ◽  
Ilare Bordeasu ◽  
Ion Dragos Utu ◽  
Ion Mitelea
Keyword(s):  
Pure Titanium ◽  
Titanium Substrate ◽  
High Strength ◽  
Biological Properties ◽  
Commercially Pure Titanium ◽  
Hvof Spraying ◽  
Before And After ◽  
Sbf Solution ◽  
Oxygen Fuel

Hydroxyapatite (HA) is a very common material used for biomedical applications. Usually, in order to improve its poor mechanical properties is combined or coated with other high-strength materials.The present paper reports the manufacturing and the biocompatibility behaviour of two different biocomposite coatings consisting of alumina (Al2O3) and hydroxyapatite (HA) using the high velocity oxygen fuel (HVOF) spraying method which were deposited onto the surface of a commercially pure titanium substrate. The biological properties of the Al2O3-HA materials were evaluated by in vitro studies. The morphology of the coatings before and after their immersing in the simulated body fluid (SBF) solution was characterized by scanning electron microscopy (SEM). The results showed an important germination of the biologic hydroxyapatite crystallite on the surface of both coatings.

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