scholarly journals In-vitro bioactivity evaluation of titanium layers manufactured on implants with LENS method

Mechanik ◽  
2017 ◽  
Vol 90 (1) ◽  
pp. 48-49
Author(s):  
Bartłomiej Wysocki ◽  
Agata Supeł ◽  
Tomasz Durejko ◽  
Wojciech Święszkowski
Keyword(s):  
Body Fluid ◽  
Hip Prosthesis ◽  
Calcium Phosphates ◽  
Human Blood Plasma ◽  
In Vitro Bioactivity ◽  
Laser Engineering ◽  
Sbf Solution ◽  
Net Shaping ◽  
Scanning Electron

Laser Engineering Net Shaping (LENS), which is one of the 3DP techniques, allows for both, the fabrication and the modification of surface layer of the product previously prepared. In the current work, using LENS technique, highly developed bioactive layers, were prepared on the surface of the hip prosthesis. Both, fabricated layers and hip prosthesis, were fabricated from Ti-6Al-4V alloy. The tests were carried out using Simulated Body Fluid (SBF) solution, having all the necessary components on the inorganic human blood plasma. Scanning Electron Microscopy (SEM) showed the nucleation of calcium phosphates even after 2 days of immersion. Based on the results obtained in SEM and EDX tests, it can be claimed that Ti-6Al-4V layers manufactured using LENS technique exhibit high bioactivity in in-vitro, in the SBF solution.

Bioactive Composite Materials for Bone Tissue Applications

2006 ◽  
Vol 514-516 ◽  
pp. 985-989
Author(s):  
B.J.M. Leite Ferreira ◽  
M.G.G.M. Duarte ◽  
M. Helena Gil ◽  
Rui N. Correia ◽  
J. Román ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Bone Tissue ◽  
Tissue Repair ◽  
Body Fluid ◽  
In Vitro Bioactivity ◽  
X Ray Diffraction ◽  
X Ray ◽  
Biodegradable Composite ◽  
Scanning Electron

Two materials with potential application in bone tissue repair have been developed: 1) a non-biodegradable composite based in a new methacrylic-co-acrylic matrix; and 2) a biodegradable composite based in a chitosan (Ch) matrix. Both matrices were reinforced with glass-ceramic particles of composition (mol%) 70 SiO2 – 30 CaO. The in vitro bioactivity of composites was assessed by soaking in simulated body fluid (SBF) for periods of up to 7 days at 37º C. X-ray diffraction (XRD) and scanning electron microscopy coupled with X-ray energy dispersive spectroscopy (SEM-EDS) were used for deposit identification after different soaking periods. Calcium phosphate particulate deposits were detected after 3 days of immersion, followed by growth and maturation towards apatite.

Carbonate Substitution and Preferred Growth Orientation of Biomimetic Apatites

2007 ◽  
Vol 330-332 ◽  
pp. 725-728 ◽  
Author(s):  
Frank A. Müller ◽  
Lenka Müller ◽  
Daniel Caillard ◽  
Egle Conforto
Keyword(s):  
Titanium Surface ◽  
Calcium Phosphates ◽  
Human Blood Plasma ◽  
In Vitro Bioactivity ◽  
Cross Sectional ◽  
Growth Orientation ◽  
Carbonated Apatite ◽  
Carbonate Substitution ◽  
Type Substitution

Simulated body fluid (SBF) solutions are widely used for in vitro bioactivity tests and to coat bioinert materials with biomimetic calcium phosphates. In this study SBF solutions with varying HCO3 - content were used to precipitate hydroxy carbonated apatite (HCA) on a bioactive titanium surface. XRD as well as cross-sectional TEM analyses revealed that the biomimetically derived crystallites show a preferred growth orientation in direction of their c-axis and perpendicular to the surface of the substrate. FTIR and Raman analyses revealed that, as long as the HCO3 - concentration in the testing solutions is below 20 mmol/l, only B-type HCA precipitates. Using SBF with a HCO3 - concentration equal to human blood plasma (27 mmol/l) leads to a AB-type substitution where, in accordance with bone mineral, CO3 2- substitutes PO4 3- as well as OH-.

scholarly journals Preparation, characterization and in vitro bioactivity of polyvinyl alcohol-hydroxyapatite biphasique membranes

2013 ◽  
Vol 6 (1) ◽  
pp. 8-14 ◽  
Author(s):  
Zuzana Balgová ◽  
Martin Palou ◽  
Jaromír Wasserbauer ◽  
Gabriela Lutišanová ◽  
Jana Kozánkováb
Keyword(s):  
Hydrogen Bond ◽  
Fourier Transform ◽  
Polyvinyl Alcohol ◽  
Weight Percent ◽  
Surface Microstructure ◽  
In Vitro Bioactivity ◽  
Before And After ◽  
Sbf Solution ◽  
Scanning Electron

Abstract Six membranes of polyvinyl alcohol (PVA) with various weight percent - 0 %, 10 %, 20 %, 30 %, 40 % and 50 % of hydroxyapatite (HA) were prepared. Fourier Transform Infrared (FTIR) spectroscopy was used to identify the different functional groups in membrane composites. The surface morphology was examined through scanning electron microscope. The in vitro bioactivity tests in Simulated Blood Fluid (SBF) have been performed up to 28 days, especially for membrane containing 50 wt. % HA. SEM was used to characterize surface microstructure of biocomposite membranes before and after immersion in SBF. It was observed the formation of clusters within membranes with increasing amount of HA particles due to hydrogen bond and also the agglomeration and crystal growth of HA particles during drying of membranes. The bioactivity was found increasing with time immersion of biocomposite materials in SBF solution.

Study of Phosphocalcic Glasses from SiO2-CaO-P2O5 System with and Without Silver II. The bioactivity analysis by FTIR, SEM methods and microbiological study of silver-doped glasses

2017 ◽  
Vol 68 (6) ◽  
pp. 1188-1192
Author(s):  
Daniela Avram ◽  
Nicolae Angelescu ◽  
Dan Nicolae Ungureanu ◽  
Ionica Ionita ◽  
Iulian Bancuta ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Body Fluid ◽  
Pathogenic Bacteria ◽  
De Novo ◽  
Microbiological Examination ◽  
Doped Glasses ◽  
P2o5 System ◽  
Scanning Electron

The study in vitro of the glass powders bioactivity was performed by soaking them in simulated body fluid for 3 to 21 days at a temperature of 37�C and pH = 7.20. The synthesis de novo of hydroxyapatite, post soaking was confirmed by Fourier Transform Infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The study of the antimicrobial activity was performed by microbiological examination on two strains of pathogenic bacteria involved in postoperative nosocomial infections.

Mn Blended Hydroxyapatite Nanoceramic: Bioactivity, Dielectric and Luminescence Studies

2013 ◽  
Vol 18 ◽  
pp. 43-59
Author(s):  
Megha Mahabole ◽  
Manjushree Bahir ◽  
Rajendra Khairnar
Keyword(s):  
Dielectric Constant ◽  
Calcium Ions ◽  
Nucleation And Growth ◽  
Partial Replacement ◽  
Apatite Structure ◽  
Manganese Ions ◽  
Photoluminescence Properties ◽  
In Vitro Bioactivity ◽  
Sbf Solution

Abstract: In this study, in-vitro bioactivity of manganese blended hydroxyapatite (Mn-HAp) pellets is carried out using simulated body fluid (SBF) solution. The incubated Mn-HAp samples are characterized by XRD, FTIR and SEM/EDAX. Dielectric and photoluminescence properties of Mn-HAp samples are studied as a function of incubation period in SBF. XRD profiles show that hexagonal apatite structure remains intact after partial replacement of calcium ions by manganese ions and even after incubation. The change in absorption due to phosphate group, depicted in FTIR spectra, for incubated samples confirms growth of apatite on Mn-HAp surface. SEM/ EDAX studies suggest that Mn-HAp surface promotes the growth of apatite without changing its structure due to apatite nucleation and growth on the surface of Mn-HAp. The value of dielectric constant of Mn-HAp increases after incubation. Increase in period of immersion in m-SBF leads to decrease in dielectric constant of manganese exchanged hydroxyapatite. The photoluminescence (PL) study reveals that the Mn-HAp can be used stable and efficient blue luminescent material.

scholarly journals Effects of Mullite, Maghemite, and Silver Nanoparticles Incorporated in β-Wollastonite on Tensile Strength, Magnetism, Bioactivity, and Antimicrobial Activity

Materials ◽  
2021 ◽  
Vol 14 (16) ◽  
pp. 4643
Author(s):  
Hamisah Ismail ◽  
Farah ‘Atiqah Abdul Azam ◽  
Zalita Zainuddin ◽  
Hamidun Bunawan ◽  
Muhamad Afiq Akbar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Tensile Strength ◽  
Body Fluid ◽  
Rice Husk Ash ◽  
Antibacterial Properties ◽  
Bone Defects ◽  
Antibacterial Efficacy ◽  
In Vitro Bioactivity ◽  
Defect Recovery

β-wollastonite (βW) has sparked much interest in bone defect recovery and regeneration. Biomaterial-associated infections and reactions between implants with human cells have become a standard clinical concern. In this study, a green synthesized βW, synthesized from rice husk ash and a calcined limestone precursor, was incorporated with mullite, maghemite, and silver to produce β wollastonite composite (βWMAF) to enhance the tensile strength and antibacterial properties. The addition of mullite to the βWMAF increased the tensile strength compared to βW. In vitro bioactivity, antibacterial efficacy, and physicochemical properties of the β-wollastonite and βWMAF were characterized. βW and βWMAF samples formed apatite spherules when immersed in simulated body fluid (SBF) for 1 day. In conclusion, βWMAF, according to the tensile strength, bioactivity, and antibacterial activity, was observed in this research and appropriate for the reconstruction of cancellous bone defects.

scholarly journals The effect of calcinated hydroxyapatite and magnesium doped hydroxyapatite as fillers on the mechanical properties of a model BisGMA/TEGDMA dental composite initially and after aging

10.30544/403 ◽  
2018 ◽  
Vol 24 (4) ◽  
Author(s):  
Tamara Matic ◽  
Maja Ležaja Zebić ◽  
Ivana Cvijović-Alagić ◽  
Vesna Miletić ◽  
Rada Petrović ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Body Fluid ◽  
Control Sample ◽  
Dental Composite ◽  
In Vitro Bioactivity ◽  
Significant Difference ◽  
Nanosized Hydroxyapatite ◽  
Glass Fillers ◽  
Mg Doped

The aim of this study was to investigate the possibility of modifying model BisGMA/TEGDMA dental composite by substituting 10 wt. % of conventional glass fillers with bioactive fillers based on calcinated nanosized hydroxyapatite (HAp) and Mg doped hydroxyapatite (Mg-HAp). HAp and Mg-HAp powders were synthesized hydrothermally. Mechanical properties: hardness by Vickers (HV) and flexural strength (Fs) were tested initially and after being stored for 28 days in simulated body fluid (SBF). The experimental composites with HAp and Mg-HAp particles showed no statistically significant difference in HV compared to the control (p>0.05) either initially or after storage. Although mean Fs values of modified composites tested initially were lower (62 MPa) than those of the control (72 MPa), after 28 days of storage in SBF Fs values were greater for modified composites (42 MPa control sample, 48 MPa HAp and Mg-HAp samples). In vitro bioactivity of BisGMA/TEGDMA composites with HAp and Mg-HAp particles after 28 days in SBF was not detected. Keywords: hydroxyapatite; magnesium; dental composite; mechanical properties;

Synthesis of sol-gel derived glass powder and in vitro bioactivity property tested in simulated body fluid

2016 ◽  
Author(s):  
S. A. Syed Nuzul Fadzli ◽  
S. Roslinda ◽  
Firuz Zainuddin ◽  
Hamisah Ismail
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Glass Powder ◽  
Sol Gel ◽  
In Vitro Bioactivity

scholarly journals Organic/Inorganic bioactive materials Part III: in vitro bioactivity of gelatin/silicocarnotite hybrids

2009 ◽  
Vol 7 (4) ◽  
pp. 721-730 ◽  
Author(s):  
Lachezar Radev ◽  
Maria Fernandes ◽  
Isabel Salvado ◽  
Daniela Kovacheva
Keyword(s):  
Calcium Phosphate ◽  
Hybrid Materials ◽  
Calcium Phosphates ◽  
Ceramic Powder ◽  
Structure Evolution ◽  
In Vitro Test ◽  
In Vitro Bioactivity ◽  
Bioactive Materials ◽  
Vitro Test

AbstractIn this work we present our experimental results on synthesis, structure evolution and in vitro bioactivity assessment of new gelatin/silicocarnotite hybrid materials. The hybrids were obtained by diluting gelatin (G) and silicocarnotite (S) ceramic powder with G:S ratios of 75:25 and 25:75 wt.% in hot (40°C) water. The hybrids were characterized using XRD, FTIR, SEM/EDS and XPS. FTIR depicts that the “red shift” of amide I and COO− could be attributed to the fact that the gelatin prefers to chelate Ca2+ from S. The growth of calcium phosphates on the surface of the hybrids synthesized and then immersed in 1.5 SBF for 3 days was studied by using of FTIR, XRD and SEM/EDS. According to FTIR results, after an immersion of 3 days, A and B-type CO3HA can be observed on the surface. XRD results indicate the presence of hydroxyapatite with well defined crystallinity. SEM/EDS of the precipitated layers show the presence of CO3HA and amorphous calcium phosphate on the surface of samples with different G/S content when immersed in 1.5 SBF. XPS of the G/S hybrid with 25:75 wt.% proved the presence of Ca-deficient hydroxyapatite after an in vitro test for 3 days.

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