Productivity and quality of spring bread wheat of Myronivka breading depending on hydrothermal conditions of the year

2019 ◽  
Vol 9 (0) ◽  
pp. 91-97
Author(s):  
N. V. Vasylenko ◽  
I. V. Pravdziva ◽  
R. M. Blyzniuk ◽  
S. O. Khomenko
PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7082 ◽  
Author(s):  
Maral Utebayev ◽  
Svetlana Dashkevich ◽  
Nina Bome ◽  
Kulpash Bulatova ◽  
Yuri Shavrukov

Background Spring bread wheat (Triticum aestivum L.) represents the main cereal crop in Northern Kazakhstan. The quality of wheat grain and flour strongly depends on the structure of gluten, comprised of gliadin and glutenin proteins. Electrophoresis spectra of gliadins are not altered by environmental conditions or plant growth, are easily reproducible and very useful for wheat germplasm identification in addition to DNA markers. Genetic polymorphism of two Gli loci encoding gliadins can be used for selection of preferable genotypes of wheat with high grain quality. Methods Polyacrylamide gel electrophoresis was used to analyse genetic diversity of gliadins in a germplasm collection of spring bread wheat from Northern Kazakhstan. Results The highest frequencies of gliadin alleles were found as follows, in Gli1: -A1f (39.3%), -B1e (71.9%), and -D1a (41.0%); and in Gli-2: -A2q (17.8%), -B2t (13.5%), and -D2q (20.4%). The combination of these alleles in a single genotype may be associated with higher quality of grain as well as better adaptation to the dry environment of Northern Kazakhstan; preferable for wheat breeding in locations with similar conditions.


Author(s):  
O. A. Orlovskaya ◽  
S. I. Vakula ◽  
L. V. Khotyleva ◽  
A. V. Kilchevsky

Related wild and cultural wheat species are regularly involved for expanding T. aestivum genetic diversity because they contain many valuable genes. We evaluated the effect of the genetic material of tetraploid species of the genus Triticum (T. dicoccoides, T. dicoccum) on the grain quality of introgression lines of spring bread wheat. The composition of the high molecular weight glutenin subunits which play an essential role in the formation of wheat baking properties was identified in the introgression lines of bread wheat and their parental forms. The traits of grain quality (hardness, protein and gluten content, gluten quality) were also evaluated. The lines with Glu-1 loci alleles from wheat relatives T. dicoccoides and Т. dicoccum were selected. It was found that the introgression of alien genetic material into the common wheat genome had a positive effect on the parameters of grain quality such as hardness, protein and gluten content. The lines with Glu-A1 loci alleles from T. dicoccoides and Glu-B1 from T. dicoccum were at the level of a parent wheat variety or of a higher gluten quality. As a result of the research, the new lines of bread soft wheat with high grain quality were found and can be used in the crop breeding.


2021 ◽  
Vol 182 (1) ◽  
pp. 99-109
Author(s):  
M. U. Utebayev ◽  
N. A. Bome ◽  
E. C. Zemtsova ◽  
O. O. Kradetskaya ◽  
I. V. Chilimova

Background.Glutenin is a storage protein in wheat seeds, important for the quality of bread prepared from wheat. Studying glutenin polymorphism can help to identify valuable genotypes and promising new breeding lines for further crossings. The aim of this study was to identify subunits of glutenin and determine alleles at the Glu-1loci in the spring bread wheat germplasm collection.Materials and methods.A panel of 54 Russian and 76 Kazakh bread wheat germplasm accessions from various breeding centers was selected. Gliadin electrophoresis was carried out in a concentrating and separating polyacrylamide gel system following the Laemmli method. Glutenin subunits were identified according to the catalogue produced by Payne and Lawrence.Results and discussion.At the Glu-A1 locus, two alleles, band c, were identified, with different frequencies of occurrence among studied wheat accessions. The Glu-A1ballele occurred more frequently than Glu-A1сin the studied germplasm from all breeding centers. Meanwhile, six alleles, а, b, c, d, fand g, were found at theGlu-B1 locus. The most frequently occurring Glu-B1с allele encoded two subunits (7+9). The third homologous gene, Glu-D1, had only two identified alleles, aand d, with various frequencies among the studied wheat accessions. Wheat germplasm of various origin had specific combinations of glutenin subunits, providing different scores of grain quality. For example, the combination of glutenin subunits, 2*, 7+9 and 5+10, provided the highest score (= 9) of grain quality. A single change in the Glu-1subunit composition, 2*, 7+9 and 2+12, caused a reduction in grain quality (= 7 score); and the combination of Null, 7and 5+10 was accompanied by the lowest (= 6) grain quality. The analysis showed that two loci, Glu-A1 and Glu-B1, induced significant differences between wheat accessions originated from Tyumen and Chelyabinsk, while the accessions from Chelyabinsk and East Kazakhstan differed significantly at the Glu-A1and Glu-D1loci.


2021 ◽  
pp. 27-33
Author(s):  
Sergey Nikolaevich Sibikeev ◽  
Alexander Yevgenyevich Druzhin ◽  
Lyubov Vladimirovna Andreeva

The effects of the combinations 7DS-7DL-7Ae#1L (Lr19 gene) and 1BL-1RS (Lr26 gene) translocations, substitution of 6D(6Agi) (Lr6Agi gene) and 1BL-1RS translocation, as well as translocations of 7DS-7DL-7Ae#1L, 1BL-1RS and Lr39 gene on the bread making quality were evaluated in spring bread wheat introgressive lines. It was found that the genotype of recipient, the 1BL-1RS translocation donor, and the vegetation conditions have a great influence on the parameters quality of flour and bread. The role of selection within a single crosses combination for production of lines with good quality indicators is revealed. The high compensatory ability of the cultivar L505 and the synthetic hexaploid line with the pedigree Croc/Ae.squarosa(205)//Weaver (1BL-1RS translocation + Lr39 gene) for reducing the negative effect of the 1BL-1RS translocation on bread making quality was noted.


Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 895
Author(s):  
Samira El Hanafi ◽  
Souad Cherkaoui ◽  
Zakaria Kehel ◽  
Ayed Al-Abdallat ◽  
Wuletaw Tadesse

Hybrid wheat breeding is one of the most promising technologies for further sustainable yield increases. However, the cleistogamous nature of wheat displays a major bottleneck for a successful hybrid breeding program. Thus, an optimized breeding strategy by developing appropriate parental lines with favorable floral trait combinations is the best way to enhance the outcrossing ability. This study, therefore, aimed to dissect the genetic basis of various floral traits using genome-wide association study (GWAS) and to assess the potential of genome-wide prediction (GP) for anther extrusion (AE), visual anther extrusion (VAE), pollen mass (PM), pollen shedding (PSH), pollen viability (PV), anther length (AL), openness of the flower (OPF), duration of floret opening (DFO) and stigma length. To this end, we employed 196 ICARDA spring bread wheat lines evaluated for three years and genotyped with 10,477 polymorphic SNP. In total, 70 significant markers were identified associated to the various assessed traits at FDR ≤ 0.05 contributing a minor to large proportion of the phenotypic variance (8–26.9%), affecting the traits either positively or negatively. GWAS revealed multi-marker-based associations among AE, VAE, PM, OPF and DFO, most likely linked markers, suggesting a potential genomic region controlling the genetic association of these complex traits. Of these markers, Kukri_rep_c103359_233 and wsnp_Ex_rep_c107911_91350930 deserve particular attention. The consistently significant markers with large effect could be useful for marker-assisted selection. Genomic selection revealed medium to high prediction accuracy ranging between 52% and 92% for the assessed traits with the least and maximum value observed for stigma length and visual anther extrusion, respectively. This indicates the feasibility to implement genomic selection to predict the performance of hybrid floral traits with high reliability.


aBIOTECH ◽  
2021 ◽  
Author(s):  
Xiaojuan Ran ◽  
Tengfei Tang ◽  
Meiyue Wang ◽  
Luhuan Ye ◽  
Yili Zhuang ◽  
...  

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