Vernolide-A, a sesquiterpene lactone fromVernonia cinerea,induces apoptosis in B16F-10 melanoma cells by modulating p53 and caspase-3 gene expressions and regulating NF-κB-mediated bcl-2 activation

2011 ◽  
Vol 34 (3) ◽  
pp. 261-270 ◽  
Author(s):  
Poyil Pratheeshkumar ◽  
Girija Kuttan
Keyword(s):  
Sesquiterpene Lactone ◽  
Caspase 3 ◽  
Melanoma Cells ◽  
Gene Expressions

scholarly journals N-Acetylcysteine Alleviated the Deltamethrin-Induced Oxidative Cascade and Apoptosis in Liver and Kidney Tissues

2022 ◽  
Vol 19 (2) ◽  
pp. 638
Author(s):  
Ali Allam ◽  
Ahmed Abdeen ◽  
Hari Prasad Devkota ◽  
Samar S. Ibrahim ◽  
Gehan Youssef ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Caspase 3 ◽  
Kidney Injury ◽  
Renal Tissue ◽  
Health Issues ◽  
Significant Protection ◽  
Gene Expressions ◽  
Molecular Alterations ◽  
Liver And Kidney ◽  
Antioxidant Markers

Deltamethrin (DLM) is a synthetic pyrethroid with anti-acaricide and insecticidal properties. It is commonly used in agriculture and veterinary medicine. Humans and animals are exposed to DLM through the ingestion of polluted food and water, resulting in severe health issues. N-acetylcysteine (NAC) is a prodrug of L-cysteine, the precursor to glutathione. It can restore the oxidant-antioxidant balance. Therefore, this research aimed to examine whether NAC may protect broiler chickens against oxidative stress, at the level of biochemical and molecular alterations caused by DLM intoxication. The indicators of liver and kidney injury in the serum of DLM-intoxicated and NAC-treated groups were examined. Furthermore, lipid peroxidation, antioxidant markers, superoxide dismutase activity, and apoptotic gene expressions (caspase-3 and Bcl-2) were investigated. All parameters were significantly altered in the DLM-intoxicated group, suggesting that DLM could induce oxidative damage and apoptosis in hepato-renal tissue. The majority of the changes in the studied parameters were reversed when NAC therapy was used. In conclusion, by virtue of its antioxidant and antiapoptotic properties, NAC enabled the provision of significant protection effects against DLM-induced hepato-renal injury.

scholarly journals Modulatory effect of Persea Americana oil against diethylnitrosamine-induced hepatotoxicity in rats: a proposed mechanism

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Omayma A. R. Abozaid ◽  
Lobna M. Anees ◽  
Gehan R. Abdel-Hamed
Keyword(s):  
Nadph Oxidase ◽  
Caspase 3 ◽  
Persea Americana ◽  
Gene Expressions ◽  
Apoptosis Pathway ◽  
Mitochondrial Apoptosis Pathway ◽  
Apoptotic Effect ◽  
Oxide Synthase ◽  
Parp 1 ◽  
Inducible Nitric Oxide

Abstract Background The purpose of this study was to investigate the effectiveness of Persea Americana (avocado) oil against diethylnitrosamine (DEN)-induced hepatotoxicity in rats. Methods For the induction of hepatotoxicity, DEN was administrated orally in a dose of 20 mg/kg B.wt for 6 successive weeks, and then the animals were gavaged with Persea Americana oil in a dose of 4 mL/kg b.wt. daily for another 6 weeks. Serum caspase-3 activity and poly (ADP-ribose) polymerase-1 (PARP-1) levels were estimated; in addition to gene expressions for NADPH oxidase, inducible nitric oxide synthase (iNOS), Bcl-2, and Bax were detected. Results The DEN-intoxicated group exhibited a remarkable increase in NADPH oxidase and iNOS expression combined with over-activation of PARP-1 and increased antiapoptotic Bcl-2 gene expression, whereas the expression of apoptotic biomarkers significantly decreased. On the other hand, treatment with Persea Americana oil significantly suppressed the elevated levels of hepatic enzymes and improved histopathological alterations in the liver. Furthermore, these groups displayed marked downregulation in NADPH oxidase and iNOS expressions. Persea Americana oil suppressed the expression of the antiapoptotic Bcl-2, activated the intrinsic mitochondrial apoptosis pathway through upregulation of pro-apoptotic Bax, and induced an obvious increase in caspase-3 activity. Moreover, Persea Americana oil administration markedly inhibited the activity of PARP-1. Conclusions This study indicated the promising potential of Persea Americana oil against DEN-induced hepatic injury through its anti-oxidative activity and pro-apoptotic effect via caspase activation and PARP-1 inhibition.

scholarly journals Development of Postoperative Pain in Patients with End-Stage Knee Osteoarthritis Is Associated with Upregulation of Genes Related to Extracellular Matrix Degradation, Inflammation, and Apoptosis Measured in the Peripheral Blood before Knee Surgery

Life ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 224
Author(s):  
Elena V. Tchetina ◽  
Kseniya E. Glemba ◽  
Galina A. Markova ◽  
Evgeniy A. Naryshkin ◽  
Elena A. Taskina ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Postoperative Pain ◽  
Joint Replacement ◽  
Peripheral Blood ◽  
Caspase 3 ◽  
Mononuclear Cells ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Gene Expressions ◽  
Joint Replacement Surgery ◽  
End Stage

Osteoarthritis (OA) pain implies an indication for joint replacement in patients with end-stage OA. However, chronic postoperative pain is observed in 10–40% of patients with OA. Here, we identified genes whose expression in the peripheral blood before surgery could denote the risk of postoperative pain development. We examined the peripheral blood of 26 healthy subjects and 50 patients with end-stage OA prior to joint replacement surgery. Pain was evaluated before surgery using the visual analog scale (VAS) index and neuropathic pain questionnaires, Douleur Neuropathique 4 Questions (DN4) and PainDETECT questionnaires. Functional activity was assessed using the Western Ontario and McMaster Universities osteoarthritis index (WOMAC). Three and six months after surgery, pain indices according to VAS of 30% and higher were considered. Metalloproteinase (MMP)-9 and tissue inhibitor of metalloproteinase (TIMP)1 protein levels were measured using ELISA in the peripheral blood mononuclear cells (PBMCs). Total RNA isolated from whole blood was analysed using quantitative real-time RT-PCR for caspase-3, MMP-9, TIMP1, cathepsins K and S, tumour necrosis factor (TNF)α, interleukin (IL)-1β, and cyclooxygenase (COX)-2 gene expression. Seventeen patients reported post-surgical pain. Expression of cathepsins K and S, caspase-3, TIMP1, IL-1β, and TNFα genes before surgery was significantly higher in these patients compared to pain-free patients with OA. Receiver-operating characteristic (ROC) curve analyses confirmed significant associations between these gene expressions and the likelihood of pain development after arthroplasty. High baseline expression of genes associated with extracellular matrix destruction (cathepsins S and K, TIMP1), inflammation (IL-1β, TNFα), and apoptosis (caspase-3) measured in the peripheral blood of patients with end-stage OA before knee arthroplasty might serve as an important biomarker of postoperative pain development.

Biological Species and Environment Study in Microsystins Causing Apotosis in Heart

2014 ◽  
Vol 886 ◽  
pp. 341-344
Author(s):  
Tong Qiu
Keyword(s):  
Caspase 3 ◽  
Apoptotic Cell ◽  
Cell Number ◽  
Biological Species ◽  
Freshwater Ecosystems ◽  
Cyanobacterial Blooms ◽  
Caspase 9 ◽  
Gene Expressions ◽  
Genes Expression ◽  
Environment Study

The occurrence of heavy cyanobacterial blooms in eutrophic freshwater ecosystems has been a worldwide problem. Microcystins, the predominant toxins of cyanobacterial blooms, are associated with mortality and illness in both animals and human. In present study, we monitored the apoptosis of heart from MCs intoxication, and evaluated the roles of main apoptosis-related genes expression in cardiotoxic effects. The results revealed that MCs exposure led to the gradually rise in apoptotic cell number. Meanwhile, Bax, Bcl-2, p53, Caspase-3 and Caspase-9 gene expressions were significantly elevated simultaneously with the extension of the time. It suggested that MCs can cause damage to heart directly.

Ursolic acid induces apoptosis through mitochondrial intrinsic pathway and caspase-3 activation in M4Beu melanoma cells

2005 ◽  
Vol 114 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Pierre-Olivier Harmand ◽  
Rapha�l Duval ◽  
Christiane Delage ◽  
Alain Simon
Keyword(s):  
Ursolic Acid ◽  
Caspase 3 ◽  
Melanoma Cells ◽  
Intrinsic Pathway

scholarly journals Fucose-Containing Sulfated Polysaccharides from Brown Seaweeds Inhibit Proliferation of Melanoma Cells and Induce Apoptosis by Activation of Caspase-3 in Vitro

Marine Drugs ◽  
2011 ◽  
Vol 9 (12) ◽  
pp. 2605-2621 ◽  
Author(s):  
Marcel Tutor Ale ◽  
Hiroko Maruyama ◽  
Hidekazu Tamauchi ◽  
Jørn D. Mikkelsen ◽  
Anne S. Meyer
Keyword(s):  
Caspase 3 ◽  
Melanoma Cells ◽  
Sulfated Polysaccharides ◽  
Brown Seaweeds

scholarly journals Apoptosis Induced by Ziziphora tenuior Essential Oil in Human Colorectal Cancer Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Mohammadreza Azimi ◽  
Jalil Mehrzad ◽  
Armita Ahmadi ◽  
Elnaz Ahmadi ◽  
Ali Ghorbani Ranjbary
Keyword(s):  
Colorectal Cancer ◽  
Essential Oil ◽  
Mtt Assay ◽  
Caspase 3 ◽  
Rna Extraction ◽  
Gastrointestinal Diseases ◽  
Control Group ◽  
Gene Expressions ◽  
Ht 29

Ziziphora (Cacotti in Persian) belongs to the Lamiaceae family (mint group) and is vastly found in Iran and Asia. This traditional medicinal plant is normally used as analgesic and for treatment of particular gastrointestinal diseases. Since colorectal cancer is one of the most common causes of death in the world and the second leading cause of cancer death among adults, there is a pressing need to inhibit this malignancy by using methods with minimal side effects. One of these methods is the use of natural resources such as medical plants. This study is aimed at investigating the expression of apoptosis-related genes in the adjacent culture of colorectal cancer epithelial cells (HT-29) with Ziziphora essential oil (ZEO). The essential oil was extracted from Ziziphora leaves, and its compounds were determined and then added to the HT-29 culture medium at different concentrations. After 24 hours, the HT-29 cells were harvested from the medium and cytotoxicity was analyzed by MTT assay. After MTT assay and determination of the percentage of apoptosis by flow cytometry, RNA extraction was performed and the expression levels of Bax, Bcl-2, caspase 3 (C3), and caspase 9 (C9) were analyzed using newly designed primers by reverse transcription (RT) qPCR method and GeniX6 software. Also, specific antibodies were used for western blot analyses of those molecules. GC analysis revealed 42 different compounds in the ZEO, including pulegone (26.65%), menthone (5.74%), thymol (5.51%), and menthol (1.02%). MTT assay showed that the concentration of 200 μg/ml of ZEO had the highest HT-29 cell death during 24 hours. After incubation with the concentration of 50 μg/ml of ZEO for 24 and 48 hours, caspase 3 and 9 gene expressions in the treated group increased compared to those in the control group ( P < 0.001 ), while the Bcl-2 expression decreased. The results showed that having anticancer compounds, ZEO can increase C3 and C9 and decrease Bcl-2 expressions, causing apoptosis in HT-29 cells in vitro. This can lead to the use of ZEO as a factor for colorectal cancer treatment.

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