scholarly journals Effect of Several Types and Concentrations of Complex Organic Compounds on Growth of Pineapple In Vitro

Akta Agrosia ◽  
2019 ◽  
Vol 22 (1) ◽  
pp. 22-28
Author(s):  
Mangara W Sianipar ◽  
Rustikawati Rustikawati ◽  
Yudhy H Bertham ◽  
Catur Herison ◽  
Mukhtasar Mukhtasar
Keyword(s):  
Organic Compounds ◽  
Coconut Water ◽  
Shoot Height ◽  
Bean Sprout ◽  
Culture Techniques ◽  
Average Value ◽  
Planting Material ◽  
Randomized Design ◽  
Complex Organic

Pineapple plants are usually propagated vegetatively by utilizing the base of the fruit (ratoone), stem buds (sucker), fruit buds (slip) and crown (crown). Propagation of plants by utilizing parts of the plant is less effective in meeting the needs because the planting material produced is small and requires a long time. An alternative to the propagation of pineapple to increase the amount of planting material is by tissue culture techniques. This study aims to obtain the best types of complex organic compounds and the right concentration for the growth of pineapple shoots in vitro. The research was conducted in June 2017 until October 2017 at the Laboratory of Agronomy at the Faculty of Agriculture, University of Bengkulu. Complete randomized design with 3 replications was used to allocate 9 treatments of complex organic compounds. The basic media used are the composition of Murashige and Skoog (MS). Treatment consists of 1) Coconut water 100 ml / l, 2) Coconut water 150 ml / l, 3) Coconut water 200 ml / l, 4) Banana porridge 100 gl / l, 5) Banana pulp 150 g / l, 6) Banana slurry 200 g / l, 7) Bean sprout extract 100 g / l, 8) Bean sprout extract 150 g / l, 9) Bean sprout extract 200 g / l. The results showed that at 16 weeks after MS media planting the addition of 100 g / l banana pulp was able to produce the highest plant height with an average of 3.45 cm. MS media with the addition of 200 ml coconut water resulted in the highest shoot height of 1.5 cm and followed by MS with the addition of 100 g / l media of banana pulp with an average of 0.9 cm. MS media with the addition of 100 ml / l of coconut water is suitable for the growth of pineapple roots with the average value of the number of roots and the highest length of roots. MS media with the addition of bean sprout extract with various concentrations is not able to stimulate shoot growth and has a growth response that tends to be slow. Keywords: Pineapple, In Vitro, Complex Organic Compounds.

scholarly journals The effect of coconut water and benzyl amino purine (BAP) addition to the growth of pineapple from Sipahutar North Sumatera in vitro condition

2018 ◽  
Vol 6 (2) ◽  
pp. 29
Author(s):  
Harifah Insani ◽  
Fauziyah Harahap ◽  
Diky Setya Diningrat
Keyword(s):  
Tissue Culture ◽  
Leaf Length ◽  
Coconut Water ◽  
Basic Medium ◽  
Shoot Height ◽  
Culture Techniques ◽  
Base Medium ◽  
Randomized Design ◽  
Benzyl Amino Purine

Background: Pineapple fruit used in this study was obtained from Sipahutar sub-district, North Tapanuli regency, North Sumatra which was a superior commodity fruit. Propagation of the Sipahutar pineapple plant was conducted vegetatively using bud stems and crown shoots, but the amount was limited to be planted in large areas. Therefore, propagation through tissue culture techniques could be used as an alternative solution.Objective: Explants source were taken from plantlets in vitro aged 3 months were grown on Murashige and Skoog (MS) basic medium with the addition of Indole Acetic Acid (IAA) 1 ppm.Methods: Sipahutar pineapple tissue culture work has been done in YAHDI Plant Tissue Culture Laboratory, Medan. The study design used a factorial completely randomized design. The first factor is coconut water with concentration of 0%, 10%, 20%, and 30%, second factor is Benzyl Amino Purine (BAP) with concentration 0 ppm, 2 ppm and 4 ppm, so there were 12 combination with 3 repetition.Result: The results showed that the addition of coconut water on MS base medium with IAA of 1 ppm gave a significant effect on the number of leaf yield and shoot height. The provision of BAP on basic MS medium with IAA of 1 ppm gave a significant effect on the number of shoots produced, the number of leaves produced, the length of the leaves, the width ofleaves and the length of shoots. The interaction of coconut water and BAP addition on MS base medium with IAA of 1 ppm gave significant effect on leaf length, leaf width and shoot length as well.Conclusions: MS + IAA basic media combined with coconut water + BAP could stimulate the growth of Sipahutar pineapple in vitro.  

scholarly journals The effect of NAA and coconut water combination on garlic (Allium sativum L.) tissue culture

2021 ◽  
Vol 905 (1) ◽  
pp. 012036
Author(s):  
Nandariyah ◽  
L S Mahmudah ◽  
R B Arniputri ◽  
A T Sakya
Keyword(s):  
Tissue Culture ◽  
Coconut Water ◽  
Emergence Time ◽  
Shoot Height ◽  
Culture Techniques ◽  
Randomized Design ◽  
Two Factors ◽  
Number Of Leaves ◽  
Tissue Culture Techniques ◽  
Number Of Shoots

Abstract Tissue culture techniques can increase the number of garlic seedlings. The purpose of this research is to determine the effect of NAA and coconut water in increasing the number of garlic seeds. This research used a Completely Randomized Design of two factors. The treatment used is NAA with concentrations of 0 ppm, 0.5 ppm, 1 ppm, 1.5 ppm, and coconut water concentrations of 0%, 10%, 20%. The variables observed were shoot emergence time, root emergence time, number of shoots, number of roots, number of leaves, shoot height, root length, and number of plantlets. The results showed that the addition of coconut water 20% without the addition of NAA in 1 bulb can produce 3.33 planlets and the results of explant propagation in 1 bulb can produce the number of shoots as many as 15.33 shoots. Giving coconut water with concentrations of 10% and 20% can increase the number of leaves, shoot height, and some planlets. The concentration of NAA 0.5 ppm can accelerate the root emergence time on garlic explant.

scholarly journals Multiplikasi Eksplan Tunas Anggrek Hitam (Coelogyne pandurata Lindl.) dengan Penambahan NAA (Naphthalene Acetic Acid) dan Ekstrak Biji Jagung (Zea mays) Secara In Vitro

2021 ◽  
Vol 11 (2) ◽  
pp. 114
Author(s):  
Nurkapita Nurkapita ◽  
Riza Linda ◽  
Zulfa Zakiah
Keyword(s):  
Tissue Culture ◽  
Zea Mays ◽  
Acetic Acid ◽  
Seed Extract ◽  
Naphthalene Acetic Acid ◽  
Shoot Height ◽  
Culture Techniques ◽  
Corn Seed ◽  
Randomized Design

(Article History: Received February 18, 2021; Revised April 27, 2021; Accepted May 19, 2021) ABSTRAKPerkembangbiakan anggrek secara generatif alami membutuhkan bantuan jamur mikoriza untuk perkecambahan biji, sedangkan usaha perbanyakan konvensional memerlukan waktu lama untuk memperoleh tanaman dalam jumlah banyak. Salah satu alternatif untuk perbanyakan anggrek hitam (Coelogyne pandurata Lindl.) adalah melalui multiplikasi tunas anggrek secara in vitro. Tujuan penelitian adalah untuk membuktikan pengaruh pemberian NAA (Naphthalene Acetic Acid) dan ekstrak biji jagung (Zea mays) terhadap multiplikasi tunas anggrek hitam. Penelitian dilakukan di Laboratorium Kultur Jaringan Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura Pontianak. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan dua faktor perlakuan. Faktor pertama adalah NAA terdiri dari 5 taraf konsentrasi yaitu A0 (0 M/ kontrol) A1 (10-7 M), A2 (10-6 M), A3 (5x10-7 M) dan A4 (5x10-6 M ) dan faktor ekstrak biji jagung (B) dengan 5 taraf konsentrasi yaitu B0 (0%), B1 (2,5%), B2 (5%); B3 (7,5%) dan B4 (10%). Pemberian kombinasi NAA dan ekstrak biji jagung berpengaruh nyata terhadap semua parameter yaitu jumlah tunas, jumlah daun, dan tinggi tunas. Hasil terbaik rerata jumlah tunas pada perlakuan A4+B4 yaitu 5x10-6M NAA+10% ekstrak biji jagung. Hasil terbaik pada rerata jumlah daun pada perlakuan A2+B2 yaitu 5x10-7M NAA+5% ekstrak biji jagung dan hasil terbaik pada rerata tinggi tunas pada perlakuan A1+B1 yaitu 10-7M NAA+2,5% ekstrak biji jagung.Kata Kunci: multiplikasi; tunas anggrek hitam; ekstrak biji jagung; NAA. ABSTRACTGenerative reproduction of orchid plants it takes a requires the help of mycorriza mushrooms for seed germination, whereas conventional propagation business takes a long time to obtain large quantities of plants. One alternative to the propagation black orchids (Coelogyne pandurata Lindl.) is required through tissue culture techniques. The purpose of this study is to find the influence and concentration corn seed extract (Zea mays) and NAA (Naphthalene Acetic Acid) on the multiplication black orchids. This research was conducted in the tissue culture laboratory Biology Department Faculty of Mathematics and Natural Sciences Tanjungpura University Pontianak. The study used a Complete Randomized Design (RAL) of factorial patterns with two treatment factors. The first factor is that the NAA consists of 5 concentration levels  A0 (0 M) A1 (10-7 M), A2 (10-6 M), A3 (5x10-7 M) and A4 (5x10-6 M ) and the second factor is that corn seed extract of 5 levels concentratio B0(0%), B1 (2,5%), B2 (5%); B3 (7,5%) and B4 (10%). The administration NAA and corn seed extract in combination has a real effect on all parameters namely the number shoots, the number leaves, and the height shoots. The best results where the average number of shoots in the treatment of A2+B2 namely 5x10-6M NAA + 10% corn seed extract. The best results average number of leaves in the treatment  A2+B2 namely 5x10-7M NAA + 5% corn seed extract and in the best results for shoot height in the treatment of A1+B1 namely 10-7M NAA + 2.5% corn seed extract.Keywords: Multiplication; black orchid’s shoot; corn  seed extract; NAA

scholarly journals Coconut Water and IAA Effect on the In Vitro Growth of Tribulus terrestris L.

2019 ◽  
Vol 67 (1) ◽  
pp. 9-18
Author(s):  
Enik Akhiriana ◽  
Samanhudi ◽  
Ahmad Yunus
Keyword(s):  
Water Concentration ◽  
Coconut Water ◽  
Tribulus Terrestris ◽  
Emergence Time ◽  
Shoot Height ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Indole 3 Acetic Acid ◽  
Number Of Shoots

Tribulus (Tribulus terrestrisL.) is a medicinal plant with considerable implementation such as aphrodisiac and anti‑inflammation drugs. This research was conducted to study the effectivity of coconut water and IAA (Indole‑3‑acetic acid) application on various concentration as the growth regulator for tribulus growth on thein vitroand its effect toward observation variables. The material used were cotyledon from tribulus embryo and MS (Murashige and Skoog) medium. The research method used a Completely Randomized Design with first factor of coconut water concentration of 0 mL L-1, 50 mL L-1, 100 mL L-1, and 150 mL L-1and IAA concentration of 0 ppm, 0.15 ppm, 0.20 ppm, and 0.25 ppm as the second factor. Observed variables were shoots emergence time, number of shoots, shoot height, number of leaves, roots emergence time, number of roots and regression between observation variables. The result showed that the combination of 150 mL L-1coconut water and 0.25 ppm IAA gave the highest shoot height, roots emergence time, and number of roots. Coconut water treatment on 150 mL L-1concentration alone gave the best result on shoots emergence time, number of shoots, and number of leaves, while IAA concentration of 0.25 ppm independently gave the highest number of leaves. Regression analysis result indicate that the number of roots has a positive correlation with shoots height, number of shoots and number of leaves.

scholarly journals Optimasi Konsentrasi Kinetin dan Benzyl Amino Purine Pada Kultur Tunas Vanili (Vanilla planifolia)

2021 ◽  
Vol 21 (1) ◽  
pp. 54-57
Author(s):  
Dyah Nuning Erawati ◽  
Yusriatul Mawaddah ◽  
Siti Humaida ◽  
Irma Wardati
Keyword(s):  
Tissue Culture ◽  
Shoot Multiplication ◽  
Culture Techniques ◽  
Planting Material ◽  
Randomized Design ◽  
Wet Weight ◽  
The Mean ◽  
Completely Randomized Design ◽  
Tissue Culture Techniques

Vanilla has a potential to be developed through tissue culture techniques to anticipate the limitations of the parent plant as a source of planting material. The in vitro propagation ability of vanilla shoots needs to be controlled with the regulation of Kinetin and Benzyl Amino Purines. The interests of this study are 1) analysis of the response of vanilla explants at several Kinetin concentrations; 2) analysis of the response of vanilla explants at several concentrations of BAP and 3) analysis of the interaction of Kinetin and BAP on the response of vanilla explants to form shoot multiplication. The research was conducted at the Tissue Culture Laboratory Politeknik Negeri Jember from June to December 2020 using a factorial Completely Randomized Design (CRD). Factor 1 was the Kinetin concentration of 0.0, 1.0, 2.0 mg.L-1 and the second factor was the concentration of BAP 0.5, 1.5, 2.5 mg.L-1. The results proved that the fastest shoot multiplication occurred on MS medium + Kinetin 2 mg.L-1 with a mean of 8.7 days after inoculation. The mean number of shoots was 7.6 shoots/explant with the highest average wet weight of 0.9 grams/explant at the addition of BAP 1.5 mg. L-1 at measurement 70 days after inoculation.

scholarly journals PRODUK BENANG SUTRA BERKUALITAS MELALUI TEKNIK SERIKULTUR DENGAN PAKAN YANG DIKEMBANGKAN SECARA IN VITRO

2018 ◽  
Vol 5 (2) ◽  
pp. 151
Author(s):  
Faradilla Faradilla ◽  
Sulfianto Alias
Keyword(s):  
Germination Rate ◽  
Production Costs ◽  
Raw Material ◽  
Shoot Height ◽  
Silk Industry ◽  
Culture Techniques ◽  
Mulberry Leaf ◽  
Best Response ◽  
Randomized Design

Samarinda woven sarong is typical sarong of Samarinda. This sarong is still using silk spun silk raw material imported from Tiongkok. The development of aquaculture is needed to obtain local silk threads in order to help to reduce production costs in the samarinda silk industry. The high quality mulberry plants are needed to support silkworm cultivation (sericulture). The qualified mulberry plants are obtained by in vitro culture techniques. The objective of this study is to obtain mulberry leaf (Morus Sp) free from disease, uniform, and to obtain quality silk threads through sericulture techniques with feeds that were propagated in vitro. The research stages consist of sterilization, Murashige and Skoog (MS) media, sub culture, observation of data analysis fund. The design used is Completely Randomized Design with single factor, ie BAP concentration (control, 0.5 mg / l, 1 mg / l and 2 mg / l). Each treatment is repeated 8 times. The results showed that administration of ZPT 2 mg / l at age 4 MST gives the best response for all observed variables. The use of ZPT BAP with various concentrations produces the germination rate, shoot height, number of shoots and number of different leaves. All treatments are unsuccessful in inducing roots.Keywords: in vitro; Murbei; SericultureSarung tenun samarinda adalah sarung khas kota Samarinda. Sarung ini masih menggunakan bahan baku sutera jenis spun silk yang diimpor dari Tiongkok. Pengembangan serikultur diperlukan untuk mendapatkan benang sutera lokal. Sehingga membantu mengurangi biaya produksi dalam industri persuteraan samarinda. Tanaman murbei yang berkualitas diperlukan untuk menunjang budidaya ulat sutera (serikultur). Tanaman murbei yang berkualitas diperoleh dengan teknik kultur in vitro. Tujuan penelitian adalah memperoleh daun murbei (Morus Sp) yang bebas penyakit dan seragam serta memperoleh benang sutera berkualitas melalui teknik serikultur dengan pakan yan diperbanyak secara in vitro. Tahapan penelitian terdiri dari sterilisasi, pembuatan media Murashige dan Skoog (MS), sub kultur, Pengamatan dana analisis data. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan faktor tunggal yaitu berbagai konsentrasi BAP (kontrol, 0,5 mg/l, 1 mg/l dan 2 mg/l) setiap perlakuan diulang 8 kali. Hasil penelitian menunjukkan bahwa pemberian ZPT 2 mg/l pada umur 4 MST memberikan respon terbaik untuk semua variabel yang diamati, Penggunaan ZPT BAP dengan berbagai konsentrasi menghasilkan waktu kecepatan bertunas, tinggi tunas dan jumlah tunas dan jumlah daun yang berbeda. Semua perlakuan tidak berhasil dalam menginduksi akarKata kunci : in vitro; murbei; serikultur

scholarly journals RESPON EKSPLAN PISANG KLUTUK (Musa paradisiaca L.) TERHADAP KONSENTRASI EKSTRAK BIJI PINANG MUDA DAN AIR KELAPA MUDA SECARA IN VITRO

2021 ◽  
Vol 35 (3) ◽  
pp. 135-142
Author(s):  
Zulkifli ◽  
Selvia Sutriana
Keyword(s):  
Tissue Culture ◽  
Culture Technique ◽  
Coconut Water ◽  
Areca Nut ◽  
Planting Material ◽  
Randomized Design ◽  
Significant Difference ◽  
Single Response ◽  
Number Of Shoots

Nurseries play an important role in banana development. The tissue culture technique is an alternative to produce quality seeds in large quantities, uniform and produced in a short time and free of pathogens. Areca nut extract is an organic material that contains tannin and can replace bicycling used as a disinfectant in tissue culture activities. Coconut water is a food reserve that contains vitamins and growth substances, so it can stimulate germination. The purpose of the study was to examine the interaction and single response of klutuk banana explants to the concentration of areca nut extract and young coconut water in vitro. This research was carried out at the Biotechnology Laboratory, Faculty of Agriculture, the Islamic University of Riau with the planting material used Pisang Klutuk weevil with the method used in a factorial Completely Randomized Design (CRD) with 4 x 4.  The first factor was seed extract Young areca nut and the second factor was Young Coconut Water. The parameters observed were the percentage of live explants, the percentage of contaminated explants, the age of shoot emergence, and the number of shoots. The observation results were analyzed statistically and continued with the further test of Honest Significant Difference (HSD) at the 5% level. It was found that the best treatment was P2K2 (20% young betel nut extract (200 ml + 800 ml distilled water) and young coconut water 20 ml/liter media), where the percentage of life was 100%, the percentage of contamination was 0%, the age of shoots appeared was 37.67 days, and the number of shoots was 5 strands.

scholarly journals Regenerasi Sorgum (Sorghum bicolor) melalui Kultur In Vitro

2021 ◽  
Vol 17 (1) ◽  
pp. 68-75
Author(s):  
Mega Silvia ◽  
Muhammad Hazmi ◽  
Hidayah Murtiyaningsih ◽  
Laras S Arum
Keyword(s):  
In Vitro Regeneration ◽  
Water Concentration ◽  
Coconut Water ◽  
F Test ◽  
Shoot Height ◽  
Garlic Powder ◽  
Randomized Design ◽  
Shoot Number ◽  
Shoot Emergence

Sorghum has great potential to be developed as a food source in Indonesia. This study aims to regenerate sweet sorghum in vitro, by adding coconut water and garlic powder to tissue culture. The experiment used a completely randomized design with two treatment factors, and with 2 replications. The first factor was the concentration of coconut water (A), consisting of: A0 (0 mL/L), A1 (50 mL/L), A2 (100 mL/L), A3 (150 mL/L), and A4 (200 mL/L). The second factor was the concentration of garlic powder (B), consisting of : B0 (0 g/L), B1 (10 g/L), B2 (20 g/L), B3 (30 g/L), and B4 (40 g/L). Data were analysis by the analysis of variance wth the F test and DMRT follow-up test at a 0,05 level. The results of the F test showed that the variables of the time of shoot emergence, shoot height at the age of 3, 12, 15, and 18 days after initiation (DAI) were not significantly affected by treatments A, B, and their interaction (A×B), also the percentage of shoot emergence in treatments A, B, and shoot height in treatments B. Variables of shoot number and shoot height at 6 DAI were affected by treatments A, B, and A×B, also the percentage of shoot emergence in treatment A, and shoot height 9 DAI in treatments A and B. DMRT test results showed that the A4 treatment gave the best results on shoot percentage, shoot height at 6 and 9 DAI, and treatment B2 on shoot number. The addition of 200 mL/L coconut water increased shoot height and 20 g/l garlic powder increased the number of shoots in this study. Keywords: coconut water, concentration, garlic, in vitro regeneration, sorghum.   ABSTRAK Sorgum memiliki potensi yang besar untuk dikembangkan sebagai sumber pangan di Indonesia. Penelitian ini bertujuan untuk meregenerasi sorgum manis secara in vitro, dari kultur jaringan. Percobaan menggunakan Rancangan Acak Lengkap dengan dua faktor perlakuan, dan dengan 2 ulangan. Faktor pertama adalah konsentrasi air kelapa (A), terdiri dari: A0 (0 mL/L), A1 (50 mL/L), A2 (100 mL/L), A3 (150 mL/L), dan A4 (200 mL/L). Faktor kedua adalah konsentrasi bubuk bawang putih (B), terdiri B0 (0 g/L), B1 (10 g/L), B2 (20 g/L), B3 (30 g/L), dan B4 (40 g/L). Data dianalisis dengan analisis ragan dengan uji F dan uji lanjut DMRT pada taraf 0,05. Hasil uji F menunjukkan bahwa variabel saat munculnya tunas, tinggi tunas pada umur 3, 12, 15, dan 18 hari setelah inisiasi (HSI) tidak nyata dipengaruhi oleh perlakuan A, B, maupun interaksinya (A×B), demikian juga persentase munculnya tunas pada perlakuan A, B, serta tinggi tunas pada perlakuan B. Variabel-variabel jumlah tunas, tinggi tunas 6 HSI dipengaruh secara nyata oleh perlakuan A, B, maupun A×B, demikian juga persentase munculnya tunas pada perlakuan A, serta tinggi tunas 9 HSI pada perlakuan A dan B. Hasil uji DMRT menunjukkan bahwa perlakuan A4 memberikan hasil terbaik pada persentase tunas, tinggi tunas 6 dan 9 HSI, serta faktor B2 pada jumlah tunas. Penambahan air kelapa 200 mL/L meningkatkan tinggi tunas dan 20 g/L bubuk bawang putih meningkatkan jumlah tunas pada penelitian ini. Kata kunci: air kelapa, bawang putih, konsentrasi, regenerasi in vitro, sorgum

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Wulan Dari Neng Gumiwang ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Root Length ◽  
Cocos Nucifera ◽  
Water Concentration ◽  
Coconut Water ◽  
Culture Bottle ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%)  dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan 

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