scholarly journals ДИНАМІКА АКТИВНОСТІ ОКРЕМИХ ФЕРМЕНТІВ СИРОВАТКИ КРОВІ ЗА ЕКСПЕРИМЕНТАЛЬНОГО ІНВАЗУВАННЯ КРОЛІВ ЗБУДНИКОМ E. STIЕDAE

2012 ◽  
pp. 122-124
Author(s):  
О. Ф. Манжос ◽  
О. О. Передера ◽  
І. В. Лавріненко ◽  
Р. В. Передера ◽  
І. А. Жерносік
Keyword(s):  
Epithelial Cells ◽  
Biliary Tract ◽  
Acute Inflammation ◽  
Clinical Signs ◽  
Biochemical Changes ◽  
Cell Integrity ◽  
Early Stages ◽  
Cytolysis Syndrome

Розвиток печінкової форми еймеріозу кролів ха-рактеризується біохімічними змінами показниківсироватки крові. Показники активності фермен-тів сироватки крові − АсАТ, АлАТ, ЛДГ, ГГТП, ЛФ− на початкових стадіях захворювання не булиспецифічними, а лише вказували на компенсаторніреакції клітин печінки та жовчовивідних шляхів.На шосту добу експерименту, незважаючи на від-сутність клінічних ознак, реєстрували підвищенняактивності АлАТ, АсАТ, ГГТП, що свідчить пропорушення структури печінки. Домінуюче значен-ня АлАТ над АсАТ у хворих кроленят на шістна-дцяту добу дослідження є наслідком розвитку го-стрих запальних процесів у паренхімі печінки. Під-вищення активності ГГТП, АлАТ, АсАТ у сироват-ці крові відповідає наявності синдрому цитолізу,що розвивається після порушення цілісності клі-тин, у яких містяться дані ферменти: гепатоци-тів і епітеліальних клітин жовчовивідних шляхів. The development of hepatic form eymeriozu rabbitscharacterized by biochemical changes in serumparameters. Indicators of enzymes of serum AST, ALT,LDH HHTP, LF in the early stages of the disease werenot specific, but only pointed to the reactions of livercells and biliary tract. At 6 th day of the experiment,despite the absence of clinical signs recorded increasedactivity of ALT, AST, HHTP, indicating violation of thestructure of the liver. Preferential increase in activity ofALT over AST in patients with rabbits at the 16 th day ofresearch is the result of acute inflammation in the liverparenchyma. Higher values HHTP, ALT, AST in serumcorresponds to the presence of cytolysis syndrome thatdevelops after disruption of cell integrity, which includes data from enzymes: hepatocytes and biliary tract epithelial cells.

scholarly journals A New Genotype of Feline Morbillivirus Infects Primary Cells of the Lung, Kidney, Brain and Peripheral Blood

Viruses ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 146 ◽  
Author(s):  
Michael Sieg ◽  
Johannes Busch ◽  
Maria Eschke ◽  
Denny Böttcher ◽  
Kristin Heenemann ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Peripheral Blood ◽  
Neutralizing Antibodies ◽  
Mononuclear Cells ◽  
Clinical Signs ◽  
Primary Cells ◽  
Peripheral Blood Mononuclear ◽  
New Genotype ◽  
Genotype 2 ◽  
Permanent Cell

Paramyxoviruses comprise a large number of diverse viruses which in part give rise to severe diseases in affected hosts. A new genotype of feline morbillivirus, tentatively named feline morbillivirus genotype 2 (FeMV-GT2), was isolated from urine of cats with urinary tract diseases. Whole genome sequencing showed about 78% nucleotide homology to known feline morbilliviruses. The virus was isolated in permanent cell lines of feline and simian origin. To investigate the cell tropism of FeMV-GT2 feline primary epithelial cells from the kidney, the urinary bladder and the lung, peripheral blood mononuclear cells (PBMC), as well as organotypic brain slice cultures were used for infection experiments. We demonstrate that FeMV-GT2 is able to infect renal and pulmonary epithelial cells, primary cells from the cerebrum and cerebellum, as well as immune cells in the blood, especially CD4+ T cells, CD20+ B cells and monocytes. The cats used for virus isolation shed FeMV-GT2 continuously for several months despite the presence of neutralizing antibodies in the blood. Our results point towards the necessity of increased awareness for this virus when clinical signs of the aforementioned organs are encountered in cats which cannot be explained by other etiologies.

Extrahepatic biliary tract obstruction: a retrospective study of 45 cases (1983-1993)

1995 ◽  
Vol 31 (6) ◽  
pp. 478-482 ◽  
Author(s):  
MA Fahie ◽  
RA Martin
Keyword(s):  
Biliary Tract ◽  
Medical Records ◽  
Serum Bilirubin ◽  
Necrotizing Pancreatitis ◽  
Elevated Serum ◽  
Clinical Signs ◽  
Pathological Process ◽  
Biliary Tract Surgery ◽  
Long Term Prognosis

The medical records of 41 dogs and four cats with either surgical or postmortem confirmation of extrahepatic biliary tract obstruction were reviewed. Clinical signs of icterus, vomiting, anorexia, or a combination of these signs were exhibited by 100% of cases. Elevated serum bilirubin was documented in 37 of 41 dogs and two of four cats (86.6%). The predominant, underlying, pathological process was extraluminal obstruction of the biliary tract due to pancreatitis (19 of 45 cases; 42.2%) or neoplasia (11 of 45 cases; 24.4%). Biliary tract surgery was performed in 29 cases, of which 12 (41.3%) recovered. Animals diagnosed with extrahepatic biliary tract obstruction had a relatively good long-term prognosis, provided they were not compromised substantially due to severe necrotizing pancreatitis or neoplasia.

scholarly journals Influenza virus infection modulates the death receptor pathway during early stages of infection in human bronchial epithelial cells

2018 ◽  
Vol 50 (9) ◽  
pp. 770-779 ◽  
Author(s):  
Sreekumar Othumpangat ◽  
Donald H. Beezhold ◽  
John D. Noti
Keyword(s):  
Epithelial Cells ◽  
Early Stage ◽  
Death Receptor ◽  
Bronchial Epithelial Cells ◽  
Host Cells ◽  
Pcr Array ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Early Stages ◽  
Infected Cells

Host-viral interaction occurring throughout the infection process between the influenza A virus (IAV) and bronchial cells determines the success of infection. Our previous studies showed that the apoptotic pathway triggered by the host cells was repressed by IAV facilitating prolonged survival of infected cells. A detailed understanding on the role of IAV in altering the cell death pathway during early-stage infection of human bronchial epithelial cells (HBEpCs) is still unclear. We investigated the gene expression profiles of IAV-infected vs. mock-infected cells at the early stage of infection with a PCR array for death receptor (DR) pathway. At early stages infection (2 h) with IAV significantly upregulated DR pathway genes in HBEpCs, whereas 6 h exposure to IAV resulted in downregulation of the same genes. IAV replication in HBEpCs decreased the levels of DR pathway genes including TNF-receptor superfamily 1, Fas-associated death domain, caspase-8, and caspase-3 by 6 h, resulting in increased survival of cells. The apoptotic cell population decreased in 6 h compared with the 2 h exposure to IAV. The PCR array data were imported into Ingenuity Pathway Analysis software, resulting in confirmation of the model showing significant modulation of the DR pathway. Our data indicate that a significant transcriptional regulation of apoptotic, necrotic, and DR genes occur at early and late hours of infection that are vital in modulating the survival of host cells and replication of IAV. These data may have provided a likely roadmap for translational approaches targeting the DR pathway to enhance apoptosis and inhibit replication of the virus.

scholarly journals AUTOPLASTIC THYMUS TRANSPLANTS

1926 ◽  
Vol 44 (4) ◽  
pp. 523-532 ◽  
Author(s):  
Henry L. Jaffe
Keyword(s):  
Epithelial Cells ◽  
Early Stages ◽  
Reticulum Cells

1. Regeneration of a thymus transplant is characterized by hypertrophy and hyperplasia of the reticulum cells, leading to the formation of small and large atypical Hassall bodies during the early stages. 2. Regeneration is usually complete by the 3rd week, when the newly formed lobules show differentiation into cortical and medullary zones, and typical Hassall bodies appear. 3. Typical Hassall's corpuscles are also derived from the reticulum epithelial cells. 4. These corpuscles have no function, being aggregates of spent reticulum cells. 5. The thymus reticulum cells are actively phagocytic, and react rapidly when noxious influences are exerted on the gland.

scholarly journals Evaluation of clinical signs, parasitemia, hematologic and biochemical changes in cattle experimentally infected with Trypanosoma vivax

2016 ◽  
Vol 25 (1) ◽  
pp. 69-81 ◽  
Author(s):  
Otavio Luiz Fidelis Junior ◽  
Paulo Henrique Sampaio ◽  
Rosangela Zacarias Machado ◽  
Marcos Rogério André ◽  
Luiz Carlos Marques ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Acute Phase ◽  
Blood Cells ◽  
White Blood Cells ◽  
Clinical Signs ◽  
Chronic Phase ◽  
Diagnostic Value ◽  
Biochemical Changes ◽  
Severe Neutropenia ◽  
Trypanosoma Vivax

Abstract Infections by Trypanosoma vivax cause great losses to livestock in Africa and Central and South Americas. Outbreaks due this parasite have been occurred with increasing frequency in Brazil. Knowledge of changes caused byT. vivax during the course of this disease can be of great diagnostic value. Thus, clinical signs, parasitemia, hematologic and biochemical changes of cattle experimentally infected by this hemoparasite were evaluated. Two distinct phases were verified during the infection – an acute phase where circulating parasites were seen and then a chronic phase where fluctuations in parasitemia were detected including aparasitemic periods. A constant reduction in erythrocytes, hemoglobin and packed cell volume (PVC) were observed. White blood cells (WBC) showed pronounced changes such as severe neutropenia and lymphopenia during the acute phase of the illness. Decreases in cholesterol, albumin, aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and increases in glucose, globulin, protein, and alkaline phosphatase (ALP) were observed. The “Lins” isolate of T. vivax showed pathogenicity for cattle, and intense parasitemia was detected in the early stages of infection. Circulating parasites were detected for about two months. The most evident laboratory abnormalities were found in WBC parameters, including thrombocytopenia.

scholarly journals POTENTIALS OF MODERN ULTRASOUND TECHNIQUES IN THE DIAGNOSIS OF PSORIATIC ARTHRITIS

2021 ◽  
Vol 19 (2) ◽  
pp. 141-146
Author(s):  
Yu. Yu. Liashko ◽  
Keyword(s):  
Psoriatic Arthritis ◽  
Clinical Signs ◽  
Clinical Manifestations ◽  
Axial Skeleton ◽  
Pathological Process ◽  
Laboratory Diagnostics ◽  
Non Invasive ◽  
Early Stages ◽  
Instrumental Methods ◽  
Early Psoriatic Arthritis

Psoriatic arthritis is a heterogeneous disease characterized by the involvement of the axial skeleton and peripheral joints and / or entheses in the pathological process. The problems of diagnosing early psoriatic arthritis are not limited to the heterogeneity of clinical manifestations of the disease. Unlike rheumatoid arthritis, there are no biomarkers for the detection of early psoriatic arthritis, and therefore verification of the diagnosis depends on the identification of specific clinical signs. Finally, the absence of psoriasis in the presence of typical clinical signs of arthritis does not permit to confirm the diagnosis in the early stages. Considering numerous difficulties of clinical and laboratory diagnostics of psoriatic arthritis, great attention is paid to non-invasive instrumental methods for diagnosing the disease at early stages. The article provides up-to-date information on the potentials of ultrasound techniques in the diagnosis of early psoriatic arthritis.

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