BIOLOGICAL CONTROL OF POTATO SOFT ROT DISEASE (Erwinia carotovora) USING Bacillus subtilis AND Pseudomonas fluorescens

Potato (Solanum tuberosum L.) is one of the commodities of potential as a source of carbohydrates and important on Indonesia's economy. One of the constraints of potato production is soft rot disease caused by Erwinia carotovora (syn. Pectobacterium carotovorum) which is needed to be controlled. One alternative control measures is the used of bacteria Bacillus subtilis and Pseudomonas fluorescens for biological control. The purpose of this research is to know the potential of B. subtilis and P. fluorescens in single and in combination to inhibit potato soft rot diseases caused by E. carotovora. The results showed that biological control agents B. subtilis and P. fluorescens in single or in combination both of them (T3 to T17) have potential in inhibiting the growth of pathogenic E. carotovora by showing the clear zone. Biological agents provides nearly the same effect if compared with the bactericide Agrept (T2). Based on the results of the statistical analysis, the treatment 11th and treatment 14th showed a clear zone wider than the Agrept treatment (T2). The treatment 11th contain combination of biological agents B. subtilis (10 cfu/ml) and P. fluorescens (105 cfu/ml), while the treatment 14th contain combination of biological agents B. subtilis (107 cfu/ml) and P. fluorescens (105 cfu/ml)

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The biological control of soft rot disease in melon caused by Erwinia carotovora subsp.carotovora using Pseudomonas fluorescens

Microbiological Research ◽

10.1016/s0944-5013(98)80021-9 ◽

1998 ◽

Vol 153 (1) ◽

pp. 55-60 ◽

Cited By ~ 6

Author(s):

Hoda H. El-Hendawy ◽

Ibrahim M. Zeid ◽

Zeinat K. Mohamed

Keyword(s):

Biological Control ◽

Pseudomonas Fluorescens ◽

Erwinia Carotovora ◽

Soft Rot ◽

Soft Rot Disease ◽

Erwinia Carotovora Subsp.Carotovora ◽

Rot Disease

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Kemampuan Bakteri Endofit Akar dan Ubi Kentang untuk Menekan Penyakit Busuk Lunak (Erwinia carotovora pv. carotovora) pada Ubi

Agrikultura ◽

10.24198/agrikultura.v27i3.10880 ◽

2016 ◽

Vol 27 (3) ◽

Author(s):

Noor Istifadah ◽

Muhamad Salman Umar ◽

Sudarjat Sudarjat ◽

Luciana Djaya

Keyword(s):

Biological Control ◽

Potato Tuber ◽

Endophytic Bacteria ◽

Erwinia Carotovora ◽

Soft Rot ◽

Soft Rot Disease ◽

Post Harvest ◽

Rot Disease

ABSTRACTThe abilities of endophytic bacteria from potato roots and tubers to suppress soft rot disease (Erwinia carotovora pv. carotovora) in potato tuberSoft rot disease caused by Erwinia carotovora pv. carotovora is one of limiting factors in cultivation and post harvest of potato. The eco-friendly control measure that can be developed for controlling the diseases is biological control. Microbes that are potential as biological control agents include endophytic bacteria. This paper discussed the results of study examining the potential of endophytic bacteria isolated from roots and tubers of potato to inhibit the growth of E. carotovora pv. carotovora in vitro and suppress soft rot disease in potato tuber. The results showed that among 24 isolates examined, four isolates of endophytic bacteria (one isolate from potato tuber and three isolates from potato roots) inhibited the growth of E. carotovora pv. carotovora in vitro with inhibition zone 3.5-6.8 mm. In the in vivo test, the isolates inhibited the soft rot disease in potato tuber by 71.5-86.4%. The isolate that tended to show relatively better inhibition in vitro and in vivo was isolate from potato tuber which is CK U3 (Lysinibacillus sp.)Keywords: Biological control, Endophytic bacteria, Post-harvest, Potato, Soft rot diseaseABSTRAKPenyakit busuk lunak yang disebabkan bakteri Erwinia carotovora pv. carotovora, merupakan salah satu kendala dalam budidaya dan pascapanen kentang. Cara pengendalian ramah lingkungan yang dapat dikembangkan untuk menekan penyakit tersebut adalah pengendalian biologi. Kelompok mikroba yang berpotensi sebagi agens pengendali biologi adalah bakteri endofit. Artikel ini mendiskusikan potensi isolat bakteri endofit yang berasal dari ubi dan akar kentang untuk menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dan menekan perkembangan penyakit busuk lunak pada ubi kentang. Hasil percobaan menunjukkan bahwa diantara 24 isolat bakteri yang diuji, terdapat empat isolat bakteri endofit (satu isolat dari ubi kentang dan tiga isolat dari akar kentang) yang dapat menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dengan zona penghambatan sebesar 3,5-6,8 mm. Pada pengujian secara in vivo, isolat-isolat tersebut dapat menekan perkembangan penyakit busuk lunak pada ubi kentang sebesar 71,5-86,4%. Isolat yang cenderung menunjukkan penghambatan relatif lebih baik secara in vitro dan in vivo adalah isolat bakteri endofit asal ubi kentang yaitu isolat CK U3 (Lysinibacillus sp.).Kata Kunci: Pengendalian biologi, Bakteri endofit, Pascapanen, Kentang, Penyakit busuk basah

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Biological Control Efficacy of Bacillus Licheniformis Hg03 Against Soft Rot Disease of Postharvest Peach

SSRN Electronic Journal ◽

10.2139/ssrn.4000312 ◽

2022 ◽

Author(s):

Yunpeng Wang ◽

Xiaoli Wang ◽

Jingfeng Zhu ◽

Huan Wei ◽

Zhipeng Ding ◽

...

Keyword(s):

Biological Control ◽

Bacillus Licheniformis ◽

Soft Rot ◽

Control Efficacy ◽

Soft Rot Disease ◽

Rot Disease

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Erwinia carotovora pv. carotovora, causal agent of soft rot disease in some crops in Puerto Rico

The Journal of Agriculture of the University of Puerto Rico ◽

10.46429/jaupr.v74i1.6532 ◽

1969 ◽

Vol 74 (1) ◽

pp. 83-92

Author(s):

Amelia Cortés-Monllor

Keyword(s):

Puerto Rico ◽

Erwinia Carotovora ◽

Soft Rot ◽

Causal Agent ◽

Bacterial Isolates ◽

Soft Rot Disease ◽

Calla Lily ◽

Pathogenicity Tests ◽

Rot Disease ◽

Antibiotic Response

The bacterium Erwinia carotovora pv. carotovora (Jones) was identified from bacterial isolates from decaying material of tobacco, tomato, tanier, head lettuce, cabbage, Dracaena spp, Pothos spp, and calla lily as the causal agent of soft rot disease. Physiological characteristics, biochemical reactions and antibiotic response were similar among strains. The ability of the isolates to produce soft rot symptoms on healthy plants when artificially inoculated was demonstrated by pathogenicity tests.

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Evaluation of the efficiency of bacteria Pseudomonas fluorescens and Azotobacter chroococcum in the control of bacterial soft rot disease caused by bacteria Pectobacterium carotovorum

Muthanna Journal for Agricultural Sciences ◽

10.52113/mjas04/8.1/5 ◽

2020 ◽

Vol 8 (1) ◽

pp. 41-50

Author(s):

Firas T. Rasheed ◽

Abdul naby A. Matrod

Keyword(s):

Pseudomonas Fluorescens ◽

Injury Rate ◽

Soft Rot ◽

Azotobacter Chroococcum ◽

The Other ◽

Average Weight ◽

Pectobacterium Carotovorum ◽

Soft Rot Disease ◽

Significant Difference ◽

Rot Disease

"The results showed the efficacy of bacteria Pseudomonas fluorescens and Azotobacter chroococcum and their mixture in inhibiting the soft rot disease caused by the bacteria Pectobacterium carotovorum and reducing the percentage of the disease, as the treatment of using pathogen and the mixture of the two types of bacteria A. chroococcum and P. fluorescens and significantly different from the other treatments as the injury rate reached 26.6%, and the results showed that the use of A.chroococcum treatment was superior to the other treatments in increasing the chlorophyll content, reaching 46.59 mg. L-1, The treatment of using bacteria A. chroococcum showed a superiority in the number of marketable tubers, reaching 4.5 tuber / plant, and the lowest number of non-marketable tubers, reaching 1.09 tuber / plant, and the highest average weight of the marketable tubers was 947.05 g / plant. in the treatment of using a mixture of A. chroococcum and P. fluorescens and the treatment of using A. chroococcum, which amounted to 928.51 g / plant, with a significant difference from all other treatments, while the treatment of using bacteria A. chroococcum gave the lowest weight in the yield of a single unmarketable plant reached 41.95 g / plant"

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Screening, Identification, Improvement, and Genetics of Resistance to Bacteria Soft Rot (Erwinia carotovora) Disease of Brassica Vegetables

HortScience ◽

10.21273/hortsci.32.3.451b ◽

1997 ◽

Vol 32 (3) ◽

pp. 451B-451

Author(s):

Jianping Ren ◽

Michael H. Dickson

Keyword(s):

Recurrent Selection ◽

Field Tests ◽

Erwinia Carotovora ◽

Soft Rot ◽

Mature Plant ◽

Disease Score ◽

Resistance Level ◽

Narrow Sense Heritability ◽

Soft Rot Disease ◽

Rot Disease

More than 700 accessions of Brassica vegetables were screened for resistance to bacterial (Erwinia) soft rot disease using a newly developed testing procedure. Dipping a needle in 2-day-old bacterial culture and pricking petioles of plants gave the most-consistent and distinguishable results in both seedling greenhouse and mature plant field tests. High humidity (100%) and warm temperature (higher than 23°C) are the two essential conditions for this test to be successful. So far, immune material has not been found. In B. rapa, less than 7% of the accessions showed some degree of resistance. High correlation was found between seedling greenhouse tests and mature plant field tests. Genetic study showed that soft rot resistance in B. rapa is a quantitative trait. The broad-sense heritability was 60% and narrow-sense heritability was 42% in the tested population. Following three cycles of recurrent selection, the resistance level in cycle three population was improved by 2.4 disease score points (1–9 scale) compared to the original parental population and the disease score of the best line in cycle 3 was 2.7 compared to a susceptible check on which the disease score was 8 under greenhouse conditions. Under field conditions, the best cycle three line scored 2.0 in comparison to the susceptible check, which scored 7. From our study, the recurrent selection works well for improving the resistance level to the soft rot disease in B. rapa.

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001 Establishment of Methods in Evaluating the Susceptibility of Chinese Cabbage (Brassica campestris spp.) to Soft Rot Disease by Erwinia

HortScience ◽

10.21273/hortsci.34.3.440 ◽

1999 ◽

Vol 34 (3) ◽

pp. 440-441

Author(s):

Won Jun ◽

Soo-Seong Lee ◽

Jongkee Kim

Keyword(s):

Chinese Cabbage ◽

Large Scale ◽

Erwinia Carotovora ◽

Soft Rot ◽

Experimental Plant ◽

Bacterial Soft Rot ◽

Breeding Lines ◽

Inoculation Methods ◽

Soft Rot Disease ◽

Rot Disease

Three inoculation methods, including cutting of a leaf, drenching, and point inoculation, were compared in an effort to screen the susceptibility of Chinese cabbage to soft rot disease caused by Erwinia carotovora subsp. carotovora. Three- to 4-week-old seedlings from 10 lines of cabbage with 16-h-old bacterial culture were routinely used. Inoculated seedlings were kept at 25.0 ± 10.0 °C for 48 h with saturated water vapor using a plastic tunnel in a greenhouse. Sixty-day-old mature plants were produced and inoculated in a greenhouse. Severity of symptoms, which were observed from percentage of plant infected was scored as from 1 to 9, representing resistant to susceptible, respectively. The correlation between seedlings and mature plants from ten lines was evaluated among the three different inoculation methods. Point inoculation gave the most significant correlation (r = 0.843, P < 0.05) between seedlings and mature plants. A good correlation was also observed between point inoculation of seedlings and drenching of mature plants (r = 0.609, P < 0.05). Cutting of a leaf of seedlings was also correlated with point inoculation of mature plants (r = 0.609, P < 0.05). This method provides the advantage of being able to keep the experimental plant alive, as only one leaf is detached. The point inoculation method is simple and relatively sensitive, so it could be used for large-scale screening for this bacterial soft rot disease. From three different evaluation assays, it was concluded that the breeding lines, C3-28, C3-29 from Cornell Univ. (Geneva, N.Y.) and the cultivar Kweonsim319 were relatively resistant to bacterial soft rot, while the Cornell breeding line CC-25 and the `Rang-no' cultivar were relatively susceptible.

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