High positive frequency of antibodies to metallothionein and heat shock protein 70 in sera of patients with metal allergy

2020 ◽  
Author(s):  
Lungwani Muungo
Keyword(s):  
Heat Shock ◽  
Human Serum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Strong Positive Correlation ◽  
Metal Allergy ◽  
Serum Samples ◽  
Group Iii ◽  
Group I ◽  
Group Ii ◽  
Positive Frequency

Two principal types of stress protein, heat shock proteins (hsps) and metallothionein (MT), are inducedin cells responding to a variety of stresses. They play an important role in protecting cells from thesestresses. However, many reports indicate that antibodies to hsps are present in human serum and areassociated with several autoimmunity diseases. Metals, which are commonly allergenic to humans,induce both MT and hsp70 (one of the hsps family). Until now, there has been no report of any antibodyto MT in human serum. In the present study, serum samples from healthy controls (Group I), andpatients suffering from atopic dermatitis without (Group II) or with (Group III) metal allergy, weremeasured for antibodies to MT and hsp70, using an enzyme-linked immunosorbent assay (ELISA).Metal allergy was confirmed by patch testing. We first found that antibody to MT exists in human serum.We also found a high positive frequency of antibody to MT (51·3%) and to hsp70 (43·6%) in the seraof Group III, compared to those of Group I (3·8% and 5·1%) or Group II (6·4% and 5·1%). Furthermore,there was a strong positive correlation between antibody to MT and antibody to hsp70 in GroupIII (P = 0·0013), but not in Group I and Group II. Our results indicate that antibody to MT exists inhuman serum, as do antibodies to hsps, and suggest

scholarly journals Detection of Immunoglobulin M Antibodies to P35 Antigen of Toxoplasma gondii for Serodiagnosis of Recently Acquired Infection in Pregnant Women

2000 ◽  
Vol 38 (11) ◽  
pp. 3967-3970 ◽  
Author(s):  
Yasuhiro Suzuki ◽  
Raymund Ramirez ◽  
Cindy Press ◽  
Shuli Li ◽  
Stephen Parmley ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Pregnant Women ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Group Iii ◽  
Igm Antibodies ◽  
Group I ◽  
Igm Elisa ◽  
Group Ii

We examined the efficiency of detection of immunoglobulin M (IgM) antibodies to a 35-kDa antigen (P35) of Toxoplasma gondiifor serodiagnosis of acute infection in pregnant women. A double-sandwich enzyme-linked immunosorbent assay (ELISA) with recombinant P35 antigen (P35-IgM-ELISA) was used for this purpose. On the basis of the clinical history and the combination of results from the toxoplasma serological profile (Sabin-Feldman dye test, conventional IgM and IgA ELISAs, and the differential agglutination test), the patients were classified into three groups: group I, status suggestive of recently acquired infection; group II, status suggestive of infection acquired in the distant past; group III, status suggestive of persisting IgM antibodies. Eighteen (90.0%) of 20 serum samples from group I patients were positive by the P35-IgM-ELISA, whereas none of the 33 serum samples from group II patients were positive. Only 4 (25.0%) of 16 serum samples from group III patients were positive by the P35-IgM-ELISA, whereas all these serum samples were positive by the conventional IgM ELISA. These results indicate that demonstration of IgM antibodies against P35 by the P35-IgM-ELISA is more specific for the acute stage of the infection than demonstration of IgM antibodies by the ELISA that uses a whole-lysate antigen preparation. Studies with sera obtained from four pregnant women who seroconverted (IgG and IgM antibodies) during pregnancy revealed that two of them became negative by the P35-IgM-ELISA between 4 and 6 months after seroconversion, whereas the conventional IgM ELISA titers remained highly positive. The P35-IgM-ELISA appears to be useful for differentiating recently acquired infection from those acquired in the distant past in pregnant women.

scholarly journals An Evaluation and Correlation of Leptin in Gingival Crevicular Fluid and Serum in Health, Gingivitis and Periodontitis

2012 ◽  
Vol 1 (2) ◽  
pp. 93-97
Author(s):  
Vinay Vadvadgi ◽  
Neeta Padmawar
Keyword(s):  
Periodontal Disease ◽  
Gingival Crevicular Fluid ◽  
Gingival Tissue ◽  
Enzyme Linked Immunosorbent Assay ◽  
Group Iii ◽  
Group I ◽  
The Mean ◽  
Group Ii ◽  
Crevicular Fluid ◽  
Mean Concentration

ABSTRACT Background and objective Plasma leptin is associated in patients with inflammatory diseases. A high concentration of leptin is associated with healthy gingival tissue. The purpose of this study was to assess the concentration of human leptin in gingival crevicular fluid (GCF) and serum within healthy and diseased gingiva, further to explore the possibility of using the levels of leptin in GCF and serum as a biochemical marker of periodontal disease progression. Materials and methods Ninety subjects were selected with age (30-39 years) and sex (15 males and 15 females) matched, to eliminate age and sex as confounders. The subjects were divided into three groups consisting of 30 subjects in each group based on the clinical and radiological parameters; healthy (group I), gingivitis (group II), periodontitis (group III), from whom the GCF samples were collected with Periopaper GCF collection strips (Proflow, Amityville, NY, USA) for 30 seconds and blood samples with 20-gauge needle syringe respectively. Leptin concentration was determined from individual GCF and serum samples by enzyme-linked immunosorbent assay (ELISA). Results The highest mean leptin concentration in GCF was observed in group I (2,664.30 pg/ml ± 324.73) and least mean leptin concentration was obtained in group III (1,309.43 pg/ml ± 202.45). The mean concentration of group II (1,639.43 pg/ml ± 344.46) was intermediate between the highest and lowest values. In contrast, the highest mean leptin concentration in serum was obtained for group III (12,086.57 pg/ml ± 1,698.23) and least mean leptin concentration was obtained for group I (8,715.09 pg/ml ± 1,649.19). The mean concentration of the group II (10,694.01 pg/ml ± 1,777.72) were intermediate between the highest and lowest values. Conclusion The results indicated a statistically significant decrease in the GCF leptin concentration and increase in serum leptin concentration as the periodontal disease progressed. How to cite this article Vadvadgi VH, Saini R, Padmawar N. An Evaluation and Correlation of Leptin in Gingival Crevicular Fluid and Serum in Health, Gingivitis and Periodontitis. Int J Experiment Dent Sci 2012;1(2):93-97.

Effect of different temperature humidity indices onantioxidant parameters in Surti buffaloes

2016 ◽  
Author(s):  
Sandhya S. Chaudhary ◽  
Arjun B. Odedara ◽  
Virendra Kumar Singh ◽  
Pankaj A. Patel ◽  
Gopal Puri ◽  
...  
Keyword(s):  
Management Practices ◽  
Total Antioxidant Status ◽  
Dry Season ◽  
Control Group ◽  
Serum Samples ◽  
Group Iii ◽  
Group I ◽  
Hot Dry Season ◽  
Antioxidant Parameters ◽  
Group Ii

The present study was conducted on 20 Surti buffalo heifers of 16 to 21 months age maintained under standard feeding and management practices. They were categorized based on their exposure to natural environment as Group I (Hot dry season: THI1), Group II- (Hot humid season: THI2) and Group III as Control group (Comfortable season: THI3). Whole blood and serum samples were collected from all the groups and analyzed for antioxidant parameters, viz., superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase, lipid peroxidation (LPO) and total antioxidant status (TAS). The temperature humidity index (THI) calculated for Group I, Group II and Group III were 81.70 (THI1), 80.30 (THI2) and 70.00 (THI3). For Group I, Group II and Group III, concentration of SOD (U) was 5.29±0.06, 4.65±0.04 and 2.94±0.09; GPx (U/ml) was 43.63±0.71, 36.40±0.56 and 25.84±1.00; catalase (µmoles/min/ml) was 1,609.17±45.93, 1,322.00±18.65 and 1,106.35±45.07; LPO (nM of MDA/ml of packed cells) was 6.00±0.13, 3.45±0.09 and 2.87±0.14 and TAS (mmol/l) was 5.18±0.11, 2.90±0.09 and 2.37±0.13, respectively. Significantly (P is less than 0.01) higher levels of SOD, GPx, catalase, TAS and LPO were observed in hot dry season (THI1) as compared to hot humid (THI2) and comfortable (THI3) seasons. Increased levels of SOD, GPx, catalase, TAS and LPO indicated that hot dry season is more stressful for animals as compared to hot humid season in Surti buffaloes as they are inhabitants of hot humid region

scholarly journals The Role of Visfatin (Adipocytokine) Biomarker in Oral Health and Diseases among Nonobese Indian Population: A Proteomic Assay

2021 ◽  
Author(s):  
Amita Coutinho ◽  
Neethu Reddy ◽  
Anirban Chatterjee ◽  
Mahamad Irfanulla Khan
Keyword(s):  
Enzyme Linked Immunosorbent Assay ◽  
Oral Diseases ◽  
Pocket Depth ◽  
Nicotinamide Phosphoribosyltransferase ◽  
Inflammatory Biomarker ◽  
Group Iii ◽  
Group I ◽  
Potential Biomarker ◽  
Group Ii

AbstractVisfatin is an adipocytokine and a potential biomarker encoded by the nicotinamide phosphoribosyltransferase gene. It belongs to the nicotinic acid phosphoribosyltransferase family and involved in various metabolic processes and aging. The aim of this study was to evaluate the role of visfatin biomarker in oral diseases like periodontitis. A total of 60 patients (20–50 years) were included in this study, and they were divided into three groups. Group I consisted of 20 subjects with healthy periodontium, group II consisted of 20 subjects with generalized moderate gingivitis, and group III consisted of 20 subjects with generalized periodontitis. The clinical periodontal parameters, including plaque index, gingival index, probing pocket depth, and clinical attachment levels, were recorded, and saliva samples were collected. Salivary visfatin concentrations were assessed using standard enzyme-linked immunosorbent assay. The results of the study showed that the visfatin concentrations were higher in patients with gingivitis and periodontitis compared with those of healthy individuals. Visfatin was found highest in group III (38.22 ± 3.38 ng/mL) followed by group II (26.66 ± 2.24 ng/mL) and the group I (25.60 ± 2.19 ng/mL). Thus, salivary visfatin is a potential inflammatory biomarker and acts as a mediator in the pathogenesis of periodontal disease and, might serve as a diagnostic and therapeutic biomarker in oral diseases like periodontitis.

scholarly journals Validation of Bartonella henselae Western Immunoblotting for Serodiagnosis of Bartonelloses in Dogs

2020 ◽  
Vol 58 (4) ◽  
Author(s):  
Pradeep Neupane ◽  
Sindhura Sevala ◽  
Nandhakumar Balakrishnan ◽  
Henry Marr ◽  
James Wilson ◽  
...  
Keyword(s):  
Bartonella Henselae ◽  
Clinical Importance ◽  
Serum Samples ◽  
Content Type ◽  
Group Iii ◽  
Rickettsia Rickettsii ◽  
Group I ◽  
Antibody Assays ◽  
Group Ii ◽  
Etiological Agents

ABSTRACT Bartonella spp. are etiological agents of life-threatening zoonotic diseases in dogs worldwide. Due to the poor sensitivity of immunofluorescent-antibody assays (IFAs), a reliable serodiagnostic test for canine bartonelloses is of clinical importance. The utility of Western blotting (WB) for the serodiagnosis of canine bartonelloses has not been critically investigated. The objective of this study was to characterize WB immunodominant proteins that could be used to confirm a serodiagnosis of bartonelloses. Using agar-grown Bartonella henselae San Antonio type 2 (SA2) whole-cell proteins, sera derived from four dog groups were tested by WB to assess immunodominant protein recognition patterns: group I consisted of 92 serum samples (10 preexposure and 82 postexposure serum samples) from 10 adult beagles experimentally inoculated with Bartonella spp., group II consisted of 36 serum samples from Bartonella PCR-positive naturally infected dogs, group III consisted of 26 serum samples from Bartonella PCR-negative and IFA-negative dogs, and group IV consisted of serum samples from 8 Brucella canis IFA-positive and 10 Rickettsia rickettsii IFA-positive dogs. Following experimental inoculation, 9 (90%) group I dogs were variably seroreactive to one or more of six specific immunodominant proteins (13, 17, 29, 50, 56, and 150 kDa). There was a strong but variable recognition of these proteins among 81% of group II dogs. In contrast, 24/26 group III dogs were not reactive to any immunodominant protein. In this study, the sensitivity and diagnostic accuracy of B. henselae SA2 WB were higher than those of B. henselae SA2 IFA testing. Some B. henselae SA2 immunodominant proteins were recognized by dogs experimentally and naturally infected with Bartonella spp. other than B. henselae. Additional research is necessary to more fully define the utility of WB for the serodiagnosis of canine bartonelloses.

scholarly journals Study of serum Ceruloplasmin, Malondialdehyde (MDA) and hs-CRP as biomarkers of severity in Psoriasis

2021 ◽  
Vol 8 (3) ◽  
pp. 70-73
Author(s):  
Prashant K. ◽  
MI Khan
Keyword(s):  
General Medicine ◽  
Sampling Technique ◽  
Strong Positive Correlation ◽  
Positive Correlation ◽  
Serum Ceruloplasmin ◽  
Group Iii ◽  
Medical College ◽  
Group I ◽  
Group Ii ◽  
Hs Crp

Background : Psoriasis is one of the common diseases diagnosed in dermatology clinics. It is characterized by erythematous and sharply demarcated papules covered by silvery micaceous scales. Although, etiology is an unclear genetic predisposition seen in 50% of cases. This study aimed to determine the role of malondialdehyde (MDA), ceruloplasmin, and C-Reactive Protein (hs-CRP) as markers of severity of the disease. Methods: N= 75 patients were selected and divided into three categories of different severity based on PASI scores. Those with PASI score of < 10 were mild cases included in group I and with PASI Scores of 11-15 were moderate cases included in group II and PASI scores >15 were in group III. N=25 age and sex-matched taken from healthy people were included in group IV controls. Results: correlation coefficient (r) values were calculated between PASI and biomarkers. Of the biomarkers, a strong positive correlation was shown by serum ceruloplasmin levels in group I and group II (r = + 0.9). Ironically the values in group III of ceruloplasmin were positive the correlation was not strong. The Malondialdehyde values in group III also showed a strong positive correlation with r values of + 0.93. In the hsCRP group, a strong positive correlation was found in group III however, a weak positive correlation was found in group I and group II. Materials and Methods: A Hospital-based Prospective study was conducted in the Department of Medicine, Santhiram medical college & general hospital for a 2 year period.Universal Sampling Technique was used for the selection of study subjects.The study population included patients admitted with fever, flank pain, and positive urine or blood cultures in the department of general medicine in Santhiram medical college and general hospital.The final sample size was 50 subjects. Conclusion: Serum ceruloplasmin was positively correlated with PASI scores and serum MDA levels were highly correlated in severe cases of psoriasis. The hs-CRP was also found to be elevated in the psoriasis cases as compared to controls however, a significant positive correlation was found in severe cases. Therefore, psoriasis severity can be mo

scholarly journals Performances of two commercially available Newcastle disease vaccines in Bangladesh: A case-control study

2012 ◽  
Vol 28 (2) ◽  
pp. 88-91
Author(s):  
M A Kashem ◽  
M A Hashem ◽  
M H B Kabir ◽  
M I Uddin ◽  
B Hasan
Keyword(s):  
Case Control Study ◽  
Control Group ◽  
Risk Of Infection ◽  
Serum Samples ◽  
Live Vaccines ◽  
Group Iii ◽  
Group I ◽  
The Mean ◽  
Group Ii ◽  
Control Study

The study was conducted to test the performances of two commercially available live vaccines named BCRDV1(VG/GA strain) and BCRDV2 (F-strain). A total of 90 day-old broiler birds were divided into three different  groups and the serum samples were collected at day 1, 7, 14, 21, 28 and 35; mean±SD of Haemgglutination  Inhibition (HI) titres (log2) were found as 8±0.00, 7.2±0.89, 6.1±0.9, 7.1±0.75, 5.7±0.72, 4.1±0.68 and 8±0.00, 6.0±0.76, 4.8±0.81, 5.8±0.83, 4.9±0.86, 3.8±0.77 in group I and group II respectively, which was significant (P< 0.01). In control (group III) the mean±SD was found to be 8±0.00, 5.8±0.87, 4.9±0.69, 4.1±0.48, 3.0±0.56 and  <2±0 respectively. The titres of control group infer that the maternal antibody usually had a tendency to decline and may pose a risk of infection. In protection test, 100% mortality were found in control group (III) but in group I and group II the mortality of birds were 6.67% and 10%, respectively. The analysis of HI titres with the target to determine the performance of two vaccines revealed that BCRDV1 vaccinated groups was able to maintain significantly higher HI titres than BCRDV2 vaccinated group. DOI: http://dx.doi.org/10.3329/bjm.v28i2.11823 Bangladesh J Microbiol, Volume 28, Number 2, December 2011, pp 88-91

scholarly journals Heterologous antigen extract in ELISA for the detection of human IgE anti-Strongyloides stercoralis

2003 ◽  
Vol 45 (5) ◽  
pp. 265-268 ◽  
Author(s):  
Julia Maria Costa-Cruz ◽  
Joaquina Madalena ◽  
Deise Aparecida de Oliveira Silva ◽  
Mônica Camargo Sopelete ◽  
Dulcinéa Maria Barbosa Campos ◽  
...  
Keyword(s):  
Intestinal Parasites ◽  
Strongyloides Stercoralis ◽  
Specific Ige ◽  
Serum Samples ◽  
Heterologous Antigen ◽  
Total Ige ◽  
Group Iii ◽  
Group I ◽  
Group Ii

Strongyloides ratti larval extract was used for the standardization of ELISA to detect genus-specific IgE in human strongyloidiasis. Forty serum samples from monoinfected patients shedding S. stercoralis larvae (Group I), 40 from patients with other intestinal parasites (Group II), and 40 from copronegative healthy subjects (Group III) were analyzed. Genus-specific IgE levels (ELISA Index: EI) were significantly higher in the group I (EI = 1.43) than groups II (EI = 0.70) and III (EI = 0.71), showing positivity rates of 55%, 2.5% and 0%, respectively. Similarly, sera from copropositive patients had significantly higher levels of total IgE (866 IU/mL) as compared to those from group II (302 IU/mL) and III (143 IU/mL). A significant positive correlation was found between levels of Strongyloides specific-IgE and total IgE in sera from patients with strongyloidiasis. In conclusion, S. ratti heterologous extract showed to be a useful tool for detecting genus-specific IgE by ELISA, contributing for a better characterization of the immune response profile in human strongyloidiasis.

scholarly journals MEMBRANE FRACTIONS FROM Strongyloides venezuelensis IN THE IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS

2015 ◽  
Vol 57 (1) ◽  
pp. 77-80 ◽  
Author(s):  
Marcelo Andreetta Corral ◽  
Fabiana Martins Paula ◽  
Maiara Gottardi ◽  
Dirce Mary Correia Lima Meisel ◽  
Pedro Paulo Chieffi ◽  
...  
Keyword(s):  
Parasitic Nematode ◽  
Enzyme Linked Immunosorbent Assay ◽  
Parasitic Diseases ◽  
Serum Samples ◽  
Group Iii ◽  
Group I ◽  
Strongyloides Venezuelensis ◽  
Immunological Diagnosis ◽  
Heterologous Antigens ◽  
I 32

Strongyloides venezuelensis is a parasitic nematode of rodents frequently used to obtain heterologous antigens for the immunological diagnosis of human strongyloidiasis. The aim of this study was to evaluate membrane fractions from S. venezuelensis for human strongyloidiasis immunodiagnosis. Soluble and membrane fractions were obtained in phosphate saline (SS and SM) and Tris-HCl (TS and TM) from filariform larvae of S. venezuelensis. Ninety-two serum samples (n = 92) were obtained from 20 strongyloidiasis patients (Group I), 32 from patients with other parasitic diseases (Group II), and 40 from healthy individuals (Group III), and were analyzed by enzyme-linked immunosorbent assay (ELISA). Soluble fractions (SS and TS) showed 90.0% sensitivity and 88.9% specificity, whereas the membrane fractions (SM and TM) showed 95.0% sensitivity and 94.4% specificity. The present results suggest the possible use of membrane fractions of S. venezuelensis as an alternative antigen for human strongyloidiasis immunodiagnosis.

Ultrastructural Lesions Produced by Alcohol and Sucrose in the Heart and Liver of C57BL Mice

1970 ◽  
Vol 28 ◽  
pp. 208-209
Author(s):  
K.K. SEKHRI ◽  
C.S. ALEXANDER ◽  
H.T. NAGASAWA
Keyword(s):  
Osmium Tetroxide ◽  
Male Mice ◽  
Alcohol Group ◽  
Group Iii ◽  
Group I ◽  
C57bl Mice ◽  
Group Ii

C57BL male mice (Jackson Lab., Bar Harbor, Maine) weighing about 18 gms were randomly divided into three groups: group I was fed sweetened liquid alcohol diet (modified Schenkl) in which 36% of the calories were derived from alcohol; group II was maintained on a similar diet but alcohol was isocalorically substituted by sucrose; group III was fed regular mouse chow ad lib for five months. Liver and heart tissues were fixed in 2.5% cacodylate buffered glutaraldehyde, post-fixed in 2% osmium tetroxide and embedded in Epon-araldite.

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