scholarly journals Antidiabetic potentials of Syzygium guineense methanol leaf extract

2016 ◽  
Vol 5 (4) ◽  
pp. 150-156
Author(s):  
Ifeoma Chinwude Ezenyi ◽  
◽  
Oluchi Nneka Mbamalu ◽  
Lucy Balogun ◽  
Liberty Omorogbe ◽  
...  
Keyword(s):  
Glucose Tolerance ◽  
Leaf Extract ◽  
Density Lipoprotein ◽  
Hepatic Glycogen ◽  
Oral Glucose ◽  
Dependent Manner ◽  
Oral Glucose Tolerance ◽  
Control Serum ◽  
Syzygium Guineense ◽  
Alpha Glucosidase

This study examines the effects of a methanol extract of Syzygium guineense leaves in streptozotocin (STZ) - induced diabetes, evaluates its effect on alpha glucosidase and 2, 2-diphenyl-1-picrylhydrazyl radical. Diabetes was induced in rats by a single intraperitoneal injection of streptozotocin (60 mg/kg). An oral glucose tolerance test was performed after diabetes induction and repeated after 14 days of treatment with the extract. The extract elicited antihyperglycemic action in diabetic rats evidenced by an improved oral glucose tolerance. A dose of 250 mg/kg of extract significantly (P<0.01, 0.001) enhanced glucose clearance at the end of treatment period and was comparable with metformin, the group also showed increase in hepatic glycogen content by 33.9% relative to the diabetic control. Serum biochemical analysis showed that the extract improved indices of renal and hepatic function by reduction in serum albumin, creatinine, liver enzymes, total and direct bilirubin. Similarly, the extract reduced serum cholesterol, triglycerides and high density lipoprotein (HDL) in a non-dose dependent manner; treatment with 250 mg/kg extract caused significant (P<0.05) reduction of HDL. Groups which received 250 and 500 mg/kg of extract showed reversal of glomerular damage compared with the diabetic untreated group. The extract also exhibited concentration-dependent antioxidant activity (EC50= 0.2 mg/ml) and statistically significant (P<0.01, 0.001) alpha glucosidase inhibitory effect (IC50= 6.15 mg/ml). These findings show the antidiabetic potential of S. guineense leaf extract, likely mediated through its ability to inhibit alpha glucosidase, scavenge free radicals and increase intrahepatic glucose uptake and storage.

scholarly journals Characterization of the Antidiabetic Role ofParkinsonia aculeata(Caesalpineaceae)

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Ana Catarina Rezende Leite ◽  
Tiago Gomes Araújo ◽  
Bruno de Melo Carvalho ◽  
Maria Bernadete Souza Maia ◽  
Vera Lúcia de Menezes Lima
Keyword(s):  
Glucose Tolerance ◽  
Serum Triglyceride ◽  
Diabetic Rats ◽  
Epididymal Adipose Tissue ◽  
Hepatic Glycogen ◽  
Oral Glucose ◽  
Phytochemical Analysis ◽  
Oral Glucose Tolerance ◽  
Urinary Glucose

This paper reports the characterization of the antidiabetic role of a hydroethanolic extract fromParkinsoniaaerial parts (HEPA), in normal and alloxan-induced diabetic rats, treated with HEPA (125 and 250 mg/kg; p.o.). Oral glucose tolerance test, acute oral toxicity test and preliminary phytochemical analyses were performed. The diabetic rats treated with HEPA showed a significant reduction in serum and urinary glucose, urinary urea and triglyceride levels, as compared to the diabetic untreated group. However, in the normal treated groups, a significant reduction was found only in serum triglyceride levels. In all treated diabetic groups, an improvement in hepatic glycogen was observed, as well as a decrease in liquid intake and urinary volume, and an enhancement in the weight of skeletal muscles (soleusandextensor digitorum longus), kidneys and epididymal adipose tissue. Nevertheless, body and liver weights were ameliorated only in the diabetic group treated with HEPA (250 mg/kg). Moreover, oral glucose tolerance was higher in animals treated with HEPA, while results also showed that HEPA could be considered toxicologically safe. Phytochemical analysis revealed the presence of tanins, flavonoids and steroids in HEPA. In conclusion,P. aculeatapresents an antidiabetic activity and other beneficial effects that ameliorate diabetes and associated complications.

scholarly journals GC–MS analysis, toxicological and oral glucose tolerance assessments of methanolic leaf extract of Eucalyptus globulus

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Bamidele Stephen Ajilore ◽  
Tolulope Olamide Oluwadairo ◽  
Olubukola Sinbad Olorunnisola ◽  
Olumide Samuel Fadahunsi ◽  
Peter Ifeoluwa Adegbola
Keyword(s):  
Glucose Tolerance ◽  
Eucalyptus Globulus ◽  
Leaf Extract ◽  
Extraction Procedure ◽  
Toxic Effects ◽  
Gas Chromatography Mass Spectrometry ◽  
Oral Glucose ◽  
Oral Glucose Tolerance ◽  
Liver And Kidney

Abstract Background Eucalyptus globulus leaf has shown promising potential in its efficacy to manage some diseases but little is known about its safety and its use in the management of diabetes. This study was designed to identify the bioactive compounds present in Eucalyptus globulus leaf extract (EGLEX), assess its toxic effects and its oral glucose tolerance ability. Powdered Eucalyptus globulus leaf was extracted with methanol using standard extraction procedure. Preliminary phytochemistry, gas chromatography–mass spectrometry (GCMS) analysis of the extract, its acute and subacute toxic effects and on its glucose tolerance (in-vivo) capability were assessed using standard laboratory techniques. Results EGLEX was tested positive for the presence of alkaloids, cardiac glycosides, flavonoids, tannins, phlobatannins and terpenoids. Nine compounds were identified by GCMS analysis of the leaf extract. EGLEX (up to 300 mg/kg bwt) showed no toxicity in all the rats dosed for the period of 14 days. The histomorphological study of the liver and kidney tissues harvested from rats dosed with 2000 mg/kg bwt showed features of histoarchitectural distortions in the two tissues. EGLEX (200 mg/kg bwt) further demonstrated effective glucose utilization as insulin and metformin. Conclusions The results obtained deduced that EGLEX is safe at a lower dose of 300 mg/kg bwt but toxic at higher dose of 2000 mg/kg bwt, and that single dose (200 mg/kg bwt) of the plant extract prevented hyperglycemia in normal rats.

scholarly journals Antidiabetic Activity of Picris japonica Thunb Aqueous Extract in Diabetic KK-Ay Mice

2018 ◽  
Vol 2018 ◽  
pp. 1-5
Author(s):  
Yanmei Jia ◽  
Lirong Chen
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance ◽  
Aqueous Extract ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Hypoglycemic Effect ◽  
Oral Glucose ◽  
Oral Glucose Tolerance ◽  
Ay Mice

Objective. To evaluate the hypoglycemic effect of Picris japonica Thunb (Asteraceae) on KK-Ay mice. Methods. The hypoglycemic effect of Picris japonica aqueous extract (PJE) in a spontaneous type 2 diabetic model (KK-Ay mice) was studied in the present research. PJE was administrated at doses of 700 mg/kg and 350 mg/kg (calculated as crude herb) for 14 days and blood glucose, oral glucose tolerance test, plasma insulin level, and blood lipid were evaluated. Meanwhile, Rosiglitazone was used for the positive control. Results. It was found the PJE treatment significantly reduced blood glucose level and improved oral glucose tolerance ability (p < 0.01 or p < 0.05) in a dose-dependent manner compared to the control diabetic mice. The blood insulin levels were significantly reduced in PJE-treated mice (700 mg/kg) and Rosiglitazone compared with the diabetic control (p < 0.01). Compared with the control diabetic group, the serum total cholesterol, triglyceride, and low density lipoprotein cholesterol were reduced by PJE (700 mg/kg) and Rosiglitazone (p < 0.05), and the serum high density lipoprotein cholesterol was significantly increased only by Rosiglitazone (p < 0.01). Conclusions. The findings demonstrate that Picris japonica has remarkable antidiabetic effect in diabetic KK-Ay mice, which suggests that Picris japonica may be beneficial to the treatment of type 2 diabetes mellitus.

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