scholarly journals Characterization of the Antidiabetic Role ofParkinsonia aculeata(Caesalpineaceae)

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Ana Catarina Rezende Leite ◽  
Tiago Gomes Araújo ◽  
Bruno de Melo Carvalho ◽  
Maria Bernadete Souza Maia ◽  
Vera Lúcia de Menezes Lima
Keyword(s):  
Glucose Tolerance ◽  
Serum Triglyceride ◽  
Diabetic Rats ◽  
Epididymal Adipose Tissue ◽  
Hepatic Glycogen ◽  
Oral Glucose ◽  
Phytochemical Analysis ◽  
Oral Glucose Tolerance ◽  
Urinary Glucose

This paper reports the characterization of the antidiabetic role of a hydroethanolic extract fromParkinsoniaaerial parts (HEPA), in normal and alloxan-induced diabetic rats, treated with HEPA (125 and 250 mg/kg; p.o.). Oral glucose tolerance test, acute oral toxicity test and preliminary phytochemical analyses were performed. The diabetic rats treated with HEPA showed a significant reduction in serum and urinary glucose, urinary urea and triglyceride levels, as compared to the diabetic untreated group. However, in the normal treated groups, a significant reduction was found only in serum triglyceride levels. In all treated diabetic groups, an improvement in hepatic glycogen was observed, as well as a decrease in liquid intake and urinary volume, and an enhancement in the weight of skeletal muscles (soleusandextensor digitorum longus), kidneys and epididymal adipose tissue. Nevertheless, body and liver weights were ameliorated only in the diabetic group treated with HEPA (250 mg/kg). Moreover, oral glucose tolerance was higher in animals treated with HEPA, while results also showed that HEPA could be considered toxicologically safe. Phytochemical analysis revealed the presence of tanins, flavonoids and steroids in HEPA. In conclusion,P. aculeatapresents an antidiabetic activity and other beneficial effects that ameliorate diabetes and associated complications.

Preliminary Phytochemical Analysis of Extract of Spermadictyon suaveolens and its Effect on Oral Glucose Tolerance in Streptozotocin Induced Diabetic Rats

2017 ◽  
Vol 9 (03) ◽  
Author(s):  
Anuradha Vivekanand Phatak ◽  
Kedar Sudhir Prabhavalkar
Keyword(s):  
Glucose Tolerance ◽  
Glucose Tolerance Test ◽  
Tolerance Test ◽  
Diabetic Rats ◽  
Herbal Extract ◽  
Oral Glucose ◽  
Phytochemical Analysis ◽  
Oral Glucose Tolerance ◽  
Streptozotocin Diabetic Rats ◽  
Fasted Rats

The preliminary phytochemical analysis of extract of Spermadictyon suaveolens was performed and its effect on oral glucose tolerance in streptozotocin induced diabetic rats studied. 1 week after the administration of streptozotocin, diabetic rats received herbal extract orally at dose 500 mg/kg body weight for 28 consecutive days. Oral glucose tolerance test was performed on overnight fasted rats on day 28. Blood samples were collected prior to glucose administration and at 30, 60, 90, 120 minutes after glucose loading and plasma glucose level was measured for all the samples. The preliminary phytochemical analysis of extract of roots of Spermadictyon suaveolens showed the presence of carbohydrates, flavonoids, glycosides, tannins and phenolic compounds. Extract of roots of Spermadictyon suaveolens improved oral glucose tolerance in streptozotocin induced diabetic rats.

scholarly journals Pharmacological potential of methanol extract of Anacardium occidentale stem bark on alloxan-induced diabetic rats

2018 ◽  
Vol 5 (7) ◽  
pp. 2440-2454
Author(s):  
D. A. Omoboyowa ◽  
F. O. Afolabi ◽  
T. C. Aribigbola
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance ◽  
Glucose Tolerance Test ◽  
Methanol Extract ◽  
Stem Bark ◽  
Tolerance Test ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Oral Glucose Tolerance ◽  
Β Cells

Background: The anti-hyperglycemic potential of methanol stem bark extract of Anacardium occidentale (MSBEAO) was investigated using an alloxan-induced diabetic rat model. Alloxan administration induces the generation of free radicals which can affect antioxidant status resulting in the disruption of the β-cells of the pancreas. Therefore, this study examines the antioxidant potential of the plant extract and the ameliorating effect on the pancreas of alloxan-induced diabetic rats. Methods: Diabetes was induced by intraperitoneal injection of 150 mg/kg body weight of alloxan monohydrate. MSBEAO, at a concentration of 100 or 200 mg/kg b.w. was orally administered to alloxan-induced diabetic rats and normal rats. The hypoglycemic effect, oral glucose tolerance test, and biochemical assay of alloxan-induced diabetic rats were assayed using standard procedures. Results: Preliminary phytochemical screening of the extract revealed the presence of alkaloids, tannins, saponins, terpenoids, carbohydrates, and phenols at moderate concentrations. The lethality dose (LD50) of the plant extract was found to be equal to or less than 5000 mg/kg b.w. The hypoglycemic effect of the extract on the non-diabetic rats revealed a significant (p<0.05) decrease in the blood glucose concentration of animals administered with 1 g/kg b.w. of the extract, compared to normal control rats administered with normal saline. In the oral glucose tolerance test, the methanol extract exerted the highest response, similar to glibenclamide after 15 and 30 minutes of administration, compared to the control rats. The methanol extract yielded the highest blood glucose lowering effects after 9 days of treatment (p<0.05), compared to diabetic rats administered with normal saline and 0.3 mg/kg b.w. of glibenclamide. Administration of the extract at 200 mg/kg b.w. showed improved pancreas architecture and regeneration of the β-cells, compared with the pancreas of animals in the other groups. Conclusion: The results of this study suggest that MSBEAO is a potentially effective agent for the management of diabetes which might result from the antioxidant-generating capacity of the stem bark.

Effect of exercise training on glucose homeostasis in normal and insulin-deficient diabetic rats

1988 ◽  
Vol 65 (2) ◽  
pp. 844-851 ◽  
Author(s):  
L. J. Goodyear ◽  
M. F. Hirshman ◽  
S. M. Knutson ◽  
E. D. Horton ◽  
E. S. Horton
Keyword(s):  
Skeletal Muscle ◽  
Exercise Training ◽  
Glucose Tolerance ◽  
Glucose Uptake ◽  
Glucose Homeostasis ◽  
Plasma Glucose ◽  
Citrate Synthase ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Oral Glucose Tolerance

The effect of 8-wk of treadmill training on plasma glucose, insulin, and lipid concentrations, oral glucose tolerance, and glucose uptake in the perfused hindquarter of normal and streptozocin-treated, diabetic Sprague-Dawley rats was studied. Diabetic rats with initial plasma glucose concentrations of 200-450 mg/dl and control rats were divided into trained and sedentary subgroups. Training resulted in lower plasma free fatty acid concentrations and increased triceps muscle citrate synthase activity in both the control and diabetic rats; triglyceride concentrations were lowered by training only in the diabetic animals. Oral glucose tolerance and both basal and insulin-stimulated glucose uptake in hindquarter skeletal muscle were impaired in the diabetic rats, and plasma glucose concentrations (measured weekly) gradually increased during the experiment. Training did not improve the hyperglycemia, impaired glucose tolerance, or decreased skeletal muscle glucose uptake in the diabetic rats, nor did it alter these parameters in the normal control animals. In considering our results and those of previous studies in diabetic rats, we propose that exercise training may improve glucose homeostasis in animals with milder degrees of diabetes but fails to cause improvement in the more severely insulin-deficient, diabetic rat.

Antidiabetic and in vitro antioxidant effects of hydromethanol extract of Paullinia pinnata root bark in alloxan-induced diabetic rat

2017 ◽  
Vol 15 (2) ◽  
Author(s):  
Onoja Samuel Okwudili ◽  
Nwachukwu Glory Chimaobi ◽  
Ezeja Maxwell Ikechukwu ◽  
Omeh Yusuf Ndukaku
Keyword(s):  
Glucose Tolerance ◽  
Antioxidant Activities ◽  
Post Treatment ◽  
Oral Glucose ◽  
Phytochemical Analysis ◽  
Root Bark ◽  
Distilled Water ◽  
Oral Glucose Tolerance ◽  
Reference Drug

Abstract Background The study evaluated phytochemical composition, antidiabetic, oral glucose tolerance test and in vitro antioxidant activities of hydromethanol extract of Paullinia pinnata root bark. Methods Cold maceration method was used in extract preparation and scavenging of 2,2-diphenyl-1-picrylhydrazyl radicals was used to evaluate antioxidant properties of the extract. Diabetes was induced with alloxan at the dose of 160 mg/kg. The antidiabetic activity of the extract was tested at doses of 50, 100 and 200 mg/kg, and glibenclamide was used as reference drug. Results Phytochemical analysis of the extract showed the presence of alkaloids, flavonoids, glycosides, tannins, saponins and terpenes/sterols. The extract produced a significant (p<0.05) time-dependent decrease in the fasting blood glucose (FBG) levels in the treated rats when compared with the distilled water treated rats, but did not produce dose-dependent effects. The extract 50, 100 and 200 mg/kg and glibenclamide (2 mg/kg) caused 83.62 %, 60.66 %, 47.77 % and 68.52 % reduction respectively in FBG at 6 h post-treatment while the distilled water (5 mL/kg) produced 8.12 % reduction in FBG at 6 h post treatment. The extract (50 mg/kg) and glibenclamide (2 mg/kg) produced a significant (p<0.05) oral glucose tolerance effect in both normoglycemic and diabetic rats. The extract produced concentration-dependent increase in antioxidant activity and had its optimum effect at 400 µg/mL concentration. Conclusions This study suggests that P. pinnata root bark has potent antidiabetic and antioxidant activities and also validates its use in folkloric medicine in the management of diabetes-related conditions.

scholarly journals BrazilianMorus nigraAttenuated Hyperglycemia, Dyslipidemia, and Prooxidant Status in Alloxan-Induced Diabetic Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Ivanildo I. da S. Júnior ◽  
Humberto de Moura Barbosa ◽  
Débora C. R. Carvalho ◽  
Ruideglan de Alencar Barros ◽  
Flávia Peixoto Albuquerque ◽  
...  
Keyword(s):  
Glucose Tolerance ◽  
Chromatographic Analysis ◽  
Ethanolic Extract ◽  
Tolerance Test ◽  
Diabetic Rats ◽  
Atherogenic Index ◽  
Blood Levels ◽  
Oral Glucose ◽  
Oral Glucose Tolerance ◽  
Morus Nigra

Morus nigrahas been used popularly for several proposes, including diabetic. In an attempt to support medicinal value, the acute hypoglycemic, hypolipidemic, and antioxidant effects of the ethanolic extract ofMorus nigra(EEMn 200 or 400 mg/kg b.w.) were evaluated in normal and alloxan-induced diabetic treated for 14 days. Serum biochemical and antioxidant analysis were performed at the end of experiment. Oral glucose tolerance test was performed at 10th and 15th days. Chromatographic analysis by HPLC-DAD of EEMn was performed. Insulin was used as positive control to glycemic metabolism as well as fenofibrate to lipid metabolism. EEMn (400 mg/kg/day) reduced fasting and postprandial glycaemia, improved oral glucose tolerance, and reduced lipolysis and proteolysis in diabetic rats. EEMn decreased the blood levels of total cholesterol and increased HDL level when compared to the diabetic control rats. At higher levels, EEMn reduced triglycerides and VLDL levels in diabetic rats. Also, EEMn reduced malondialdehyde and increased the reduced glutathione levels in liver of diabetic rats. Chromatographic analysis identified the presence of the flavonoids rutin, isoquercetin, and kaempferitrin. Acute EEMn treatment reduced hyperglycemia, improved oral glucose tolerance, and minimized dyslipidemia and oxidative stress leading to a reduction in atherogenic index in alloxan-induced diabetic rats.

Effect ofMomordica charantia on lipid pro?le and oral glucose tolerance in diabetic rats

2004 ◽  
Vol 18 (11) ◽  
pp. 954-956 ◽  
Author(s):  
P. Chaturvedi ◽  
S. George ◽  
M. Milinganyo ◽  
Y. B. Tripathi
Keyword(s):  
Glucose Tolerance ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Oral Glucose Tolerance

Anti-hyperglycemic activity of Aegle marmelos (L.) corr. is partly mediated by increased insulin secretion, α-amylase inhibition, and retardation of glucose absorption

2017 ◽  
Vol 30 (1) ◽  
Author(s):  
Prawej Ansari ◽  
Nadia Afroz ◽  
Shahnaz Jalil ◽  
Sohel Bin Azad ◽  
Md. Gazi Mustakim ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Oral Glucose Tolerance ◽  
Plasma Insulin Concentration ◽  
Oral Sucrose ◽  
Type 2 Diabetic

AbstractBackground:(commonly known as Bael, golden apple) was formerly described to have anti-hyperglycemic activity. The present study aimed to explore the possible effects, in depth, ofMethods:This research begins with fasting blood glucose and oral glucose tolerance test (OGTT) to evaluate the primary anti-hyperglycemic effect in chemically induced type 2 diabetic rats. Furthermore, the plasma insulin concentration and serum glucose level were studied, which include measuring the sucrose content in six different segments of the gastrointestinal (GI) tract of the rats following oral sucrose feeding. An in situ, perfused, intestinal model in rats and glucose-fiber binding assay were conducted to find the effects ofResults:Treatment of extracts suppressed blood glucose elevation after oral sucrose (2.5 g/kg) administration and significantly (p<0.05) improved oral glucose tolerance in type 2 diabetic rats.Conclusions:The findings demonstrate that anti-hyperglycemic activity of

scholarly journals Antidiabetic potentials of Syzygium guineense methanol leaf extract

2016 ◽  
Vol 5 (4) ◽  
pp. 150-156
Author(s):  
Ifeoma Chinwude Ezenyi ◽  
◽  
Oluchi Nneka Mbamalu ◽  
Lucy Balogun ◽  
Liberty Omorogbe ◽  
...  
Keyword(s):  
Glucose Tolerance ◽  
Leaf Extract ◽  
Density Lipoprotein ◽  
Hepatic Glycogen ◽  
Oral Glucose ◽  
Dependent Manner ◽  
Oral Glucose Tolerance ◽  
Control Serum ◽  
Syzygium Guineense ◽  
Alpha Glucosidase

This study examines the effects of a methanol extract of Syzygium guineense leaves in streptozotocin (STZ) - induced diabetes, evaluates its effect on alpha glucosidase and 2, 2-diphenyl-1-picrylhydrazyl radical. Diabetes was induced in rats by a single intraperitoneal injection of streptozotocin (60 mg/kg). An oral glucose tolerance test was performed after diabetes induction and repeated after 14 days of treatment with the extract. The extract elicited antihyperglycemic action in diabetic rats evidenced by an improved oral glucose tolerance. A dose of 250 mg/kg of extract significantly (P<0.01, 0.001) enhanced glucose clearance at the end of treatment period and was comparable with metformin, the group also showed increase in hepatic glycogen content by 33.9% relative to the diabetic control. Serum biochemical analysis showed that the extract improved indices of renal and hepatic function by reduction in serum albumin, creatinine, liver enzymes, total and direct bilirubin. Similarly, the extract reduced serum cholesterol, triglycerides and high density lipoprotein (HDL) in a non-dose dependent manner; treatment with 250 mg/kg extract caused significant (P<0.05) reduction of HDL. Groups which received 250 and 500 mg/kg of extract showed reversal of glomerular damage compared with the diabetic untreated group. The extract also exhibited concentration-dependent antioxidant activity (EC50= 0.2 mg/ml) and statistically significant (P<0.01, 0.001) alpha glucosidase inhibitory effect (IC50= 6.15 mg/ml). These findings show the antidiabetic potential of S. guineense leaf extract, likely mediated through its ability to inhibit alpha glucosidase, scavenge free radicals and increase intrahepatic glucose uptake and storage.

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