Phytochemical Screening of Plant Extracts and GC-MS Analysis of n-Hexane Extract of the Leaves of Cassia alata Linn

2020 ◽  
Vol 9 (5) ◽  
pp. 342-347
Author(s):  
Koushik Saha ◽  
◽  
Rezwana Zaman Proma ◽  
Nasima Khan ◽  
◽  
...  

The purpose of this investigation was to undergo the preliminary phytochemical screening of the plant extracts and identify the presence of pharmacologically bioactive constituents in the leaves of Cassia alata by using gas chromatography-mass spectrometry (GC-MS) technique. The preliminary phytochemical screening of different leaf extracts of Cassia alata revealed the presence of various phytochemical compounds such as terpenoids, steroids, flavonoids, phenolic compounds, quinones, carbohydrates, tannins and alkaloids. Qualitative and quantitative determination of different biologically active compounds from the crude n-hexane extract using gas chromatography-mass spectrometry disclosed 20 compounds with varying amounts where main components were identified as palmitic acid (26.65%), stearic acid (14.27%), (E)-9-octadecadienoic acid (11.40%), erucylamide (8.34%), 1,19-eicosadiene (5.15%), stigmasterol (4.68%), linoleic acid (4.06%), vitamin E (3.97%), methyl palmitate (3.93%) and methyl 11-octadecenoate (3.32%). The compounds identified through this investigation may be responsible for any of the pharmacological properties of Cassia alata and could be of considerable interests for the development of new drug leads.

10.17158/233 ◽  
2012 ◽  
Vol 18 (1) ◽  
Author(s):  
Ma. Eva C. San Juan ◽  
Ramchand S. Jumala ◽  
Karen Hope G. Niasca

Nowadays, plant is being used as a biofuel, a probable source for a pesticide and it is valued for its pharmacological effects. Hence, the researchers engaged in the scientific approach to provide explanation to these claims. The study focused on the phytochemical screening of the ethanolic extract of radish bulbs (Raphanus sativus). It further dealt on the isolation and determination of the molecular masses to provide probable structures and associated molecular properties for its constituents.<br />Alkaloids, flavonoids, saponins and tannins were analyzed. Further, this effort involved analysis through Thin Layer Chromatography (TLC) using the mobile phase of Toluene: Chloroform (9:11) and Gas Chromatography- Mass Spectrometry (GC-MS) to analyze the first spot (Retention Factor= 0.667).<br />Phytochemical analysis showed no tannins. Using TLC, it revealed two spots having Rf values of 0.667 and 0.507, respectively. Using GC-MS method, 24 different molecules were isolated and analyzed of which only 23 were given probable structures. The 23 molecules constituted 11 long-chain alkanes, 4 long-chain alkyl esters, 2 aromatic ketones, a benzyl halide, an aryl ether, a quinone, an aromatic aldehyde, a long chain aromatic alkane, and a long-chain alkyl nitrile. With this finding, the bulb extract may be a good source of potential biofuel (mono-alkyl ester), UV A, B and C blockers in the form of diphenyl-ketone, oviposition attractants (long-chain alkanes) and also a source of bioactive molecules such as estragole which shows antispasmodic effect, methyl palmitate with immunosuppressive effect in semi-allografts and hepatoprotective activity in rats, and an analogue of the antidepressant alfetamine.


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