scholarly journals Isolation and Identification of Biosurfactant Producing Bacteria From Workshop Wastewater

2021 ◽  
Vol 9 (1) ◽  
pp. 31
Author(s):  
Asri Ainul ◽  
M. Hasbi ◽  
Eko Purwanto
Keyword(s):  
Polluted Water ◽  
Isolation And Identification ◽  
Biochemical Methods ◽  
Emulsification Index ◽  
Agar Media

Automotive workshop activities produce oil ills that may pollute waters around the workshop area. The oil-polluted water may inhabit biosurfactant producing bacteria that are able to degrade the oil. A study aimed to isolate and identify the bacteria has been conducted from July to September 2020. The bacteria samples were sampled from workshop wastewater at Kubang Raya street KM 2,5 Pekanbaru, Riau Province and sampling were conducted three times. The bacteria were isolated using TSB (Tryptone Soy Broth) and TSA (Tryptone Soy Agar) media and were identified by using biochemical methods. Results showed that there were seven types of bacteria, namely Providencia, Proteus, Acinetobacter, Bacillus, Aeromonas, Proteus and Serratia. The Emulsification index of  Providencia was 38.8%, Proteus 50%, Acinetobacter 48.8%, Bacillus 52,1%, Aeromonas 47,6%, Proteus 54,7% and Serratia 48,8%. Data obtained from this study showed that all of the identified bacteria are able to produce biosurfactants.

scholarly journals Evaluation of stx1, stx2, hlyA, and eaeA Virulence Genes in Escherichia coli O157:H7 Isolated from Meat (Beef and Mutton) in Hamedan, Iran, During 2015-2016

2020 ◽  
Vol 8 (2) ◽  
pp. 55-59
Author(s):  
Fatemeh Binandeh ◽  
Mohammadreza Pajohi-Alamoti ◽  
Pezhman Mahmoodi ◽  
Azam Ahangari
Keyword(s):  
Escherichia Coli ◽  
Virulence Genes ◽  
Escherichia Coli O157 ◽  
Isolation And Identification ◽  
E Coli ◽  
Multiplex Pcr Assay ◽  
Agar Media ◽  
Cattle Meat ◽  
Meat Samples ◽  
Encoding Genes

Background and Objectives: Consuming raw or undercooked cattle meat is the most common transmission way of infection with Escherichia coli O157:H7. The present study aimed to identify virulence genes stx1, stx2, hlyA, and eaeA in E. coli isolated from meat samples (beef and mutton) in Hamedan during 2015 and 2016. Materials and Methods: For this purpose, the swabs were randomly taken from 160 meat samples including 80 beef samples and 80 mutton samples from butcher shops. Isolation and identification of E. coli cells were conducted by culturing the swab samples on MacConkey agar and Eosin methylene blue agar media. Then, the identity of the suspected E. coli O157:H7 colonies was investigated by a multiplex PCR assay and eventually, the isolates were evaluated for the presence of stx1, stx2, hlyA, eaeA virulence genes. Results: The results showed that out of 160 cultured samples on the selective media, 60 samples (37.5%) were contaminated with E. coli. O157:H7, O157, and H7 strains were identified using PCR, among which only E. coli O157:H7 possessed all four virulence factor encoding genes. Conclusion: The results of this study showed that beef could be a reservoir for E. coli O157:H7, and it may be involved in the transmission of this pathogen to humans.

scholarly journals Identification of Malassezia species from suspected Pityriasis (versicolor) patients.

2017 ◽  
Vol 9 (2) ◽  
pp. 17-19 ◽  
Author(s):  
S.M. Ali Ahmed ◽  
Chandan Kumar Roy ◽  
Qamrul Hassan Jaigirdar ◽  
Rehana Razzak Khan ◽  
Ismet Nigar ◽  
...  
Keyword(s):  
Superficial Layer ◽  
Pityriasis Versicolor ◽  
Malassezia Furfur ◽  
Isolation And Identification ◽  
Direct Microscopy ◽  
Malassezia Species ◽  
Urease Test ◽  
Malassezia Globosa ◽  
Agar Media ◽  
Medical University

Pityriasis versicolor is a chronic, superficial fungal infection affecting the superficial layer of a stratum corneum. Malassezia furfur is the major species involved in pityriasis versicolor. Currently many researchers reported increase in the incidence of other species as a causative agent of pityriasis versicolor. Isolation and identification of Malassezia species from suspected Pityriasis versicolor patients was conducted in the Department of Microbiology and immunology Bangabandhu Sheikh Mujib Medical University (BSMMU) from September 2013 to August 2014. Ninety two clinically diagnosed patients of Pityriasis versicolor were studied and samples from skin lesion were processed for direct microscopy and culture. Species of Malassezia were identified by cultural characteristics in Dixon's agar media by macro and microscopic observation of the colonies and by catalase test, urease test, esculin test and tween assimilation test. A totalof 92 cases 70(70.08%) were positive by direct microscopy and 50(54.34%) were positive by culture. Malassezia globosa was found in 38(76%) cases as the commonest etiological agent and Malassezia furfur was found in 10(20%) cases and Malassezia obtusa in 2 (4%) cases respectively.Bangladesh J Med Microbiol 2015; 9 (2): 17-19

scholarly journals Preliminary Screening of Bio-surfactant Producing Bacteria Isolated from an Oil Contaminated Soil

2018 ◽  
pp. 1-9
Author(s):  
Kawo Abdullahi Hassan ◽  
Yahaya Sani ◽  
Olawore Yemisi Ajoke
Keyword(s):  
Optical Density ◽  
Engine Oil ◽  
Assay Method ◽  
Good Growth ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Preliminary Screening ◽  
Emulsification Index ◽  
Hemolytic Assay ◽  
Agar Media

Spent motor oil represents one of the most prominent and tenacious contaminants of soil within mechanic workshops across Nigeria. Adaptation and natural evolution proffer a rich array of a microorganism capable of producing bio-surfactants which are of high value to industry, particularly in hydrocarbon degradation. This study was undertaken to isolate and screen for bio-surfactant producing bacterial strains isolated from engine oil contaminated mechanic sites. Data obtained revealed that using 0.3% Fluconazole supplemented nutrient agar media, bacterial isolates were obtained from soil samples within Apo mechanic village, Abuja, Nigeria. Hemolytic assay method and foam capacity test were used for screening. The positive strains were grown in liquid medium and the emulsification index (EI24) was determined. A total of 3 bacterial isolates referred to as A2, B1 and C8 were positive for the hemolytic and foam capacity tests, with an emulsification index (EI24) of 40.0, 11.7 and 36.7 respectively. Growth measurements were determined by measuring optical density of the cells in broth using spectrophotometer at 605nm over a 96 hour incubatory period in nutrient broth at 4ºC, 37ºC, 42ºC. All isolates displayed mesophilic characteristics withprogressive growth. At 37ºC, isolate A2 had the highest growth rate via optical density readout 1.95, 2.11, 2.32, 2.55, measured at 605nm. Isolate A2 also the best performing isolate at 42ºC with optical density readings of 1.32, 1.00, 0.91 and 1.68, thereby suggestive of thermotolerant ability. All isolates demonstrated good growth in broth medium with pH ranging from 8.97-9.16 at 37ºC. All isolates were positive for catalase and citrate, negative for methyl red, Voges-Proskauer and indole tests. Isolate A2 was the only gram-positive, oxidase negative and non-motile bacteria. The bio-surfactants produced by the three different bacterial isolates would possess chemically distinct signatures that can be harnessed for multiple applications ranging from bioremediation to degradable detergent uses.

scholarly journals Investigation on Growth of Oil Degrading Thermophilic Bacteria Isolated from Hot Spring

2018 ◽  
Vol 15 (4) ◽  
pp. 805-815 ◽  
Author(s):  
Iqbal Ansari ◽  
Ritesh Kumar ◽  
Muniyan Sundararajan ◽  
Deblina Maiti ◽  
Mohd Ashraf Rather ◽  
...  
Keyword(s):  
Olive Oil ◽  
Oil Spills ◽  
Hot Spring ◽  
Thermophilic Bacteria ◽  
Marine Ecosystem ◽  
Polluted Water ◽  
Tween 20 ◽  
Agar Media ◽  
Offshore Oil ◽  
Petroleum Oil

Oil spills are one of the key concerns of oil industry. The main distress of environmental specialists is the hazard to the marine ecosystem, caused due to offshore oil spills. In the present study the oil degrading potential of isolated bacteria on different media composition was carried out. From the study, it has been found that oil degrading thermophilic bacteria are capable of degrading soyabean oil, olive oil, tween 20, glycerol and crude petroleum oil. The culture of thermophilic was performed in thermus agar media and it was observed from the experimental study that the growth of thermophilic bacteria was moderately good at the range of 50oC to 60oC and 60oC to 70oC but declines after 70oC; no growth was observed in the range of 25oC to 50oC. It has also detected that the decaying capability of the thermophilic bacteria in Olive oil is initially better than soyabean oil during first six hours culture afterward its performance is better in soyabean oil than Olive oil. The present study is of special environmental significance as it can be efficiently used for bioremediation of oil polluted water.

scholarly journals Salmonella from Farm to Table: Isolation, Characterization, and Antimicrobial Resistance of Salmonella from Commercial Broiler Supply Chain and Its Environment

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
M. Nasim Sohail ◽  
D. Rathnamma ◽  
S. Chandra Priya ◽  
S. Isloor ◽  
H. D. Naryanaswamy ◽  
...  
Keyword(s):  
Supply Chain ◽  
Antimicrobial Resistance ◽  
Enrichment Culture ◽  
Poultry Production ◽  
Isolation And Identification ◽  
Production Chain ◽  
Selective Enrichment ◽  
Commercial Broiler ◽  
Retail Meat ◽  
Agar Media

Antimicrobial resistance (AMR) in poultry production chain is one of the major food safety concerns due to indiscriminate usage of antibiotics and the presence of pathogens such as Salmonella which causes infections in various stages of production. In the present study, 182 samples were collected from commercial broiler supply chain, viz., three hatcheries ( n = 29 ), three commercial broiler farms (CBF; n = 99 ), and three retail meat shops (RMS; n = 54 ), and used for isolation and identification of Salmonella using three different selective agar media and a selective enrichment medium followed by PCR confirmation targeting the hilA gene. The overall prevalence of Salmonella was 47/182 (25.82%), and a significantly higher ( P < 0.05 ) prevalence was observed in retail meat shops (46.29%), CBF (19.19%), and hatcheries (10.34%). Comparison of three agar media for isolation of Salmonella revealed that all the media were equally selective. However, PCR amplification of hilA gene fragment was significantly higher ( P < 0.01 ) in selective enrichment culture tetrathionate brilliant green bile broth (TTB) as compared to all solid (agar-based) media. Susceptibility pattern against most frequently used antibiotics revealed that 100% of the isolates were resistant to at least one antibiotic. High resistance was observed for doxycycline (94.34%), followed by cefpodoxime (84.91%), ciprofloxacin (72.64%), gentamicin (65.09%), enrofloxacin (61.32%), colistin sulphate (40.42%), amikacin (34.91%), ampicillin (33.96%), neomycin (33.02), cefotaxime (30.19%), ceftazidime (29.25%), trimethoprim-sulfamethoxazole (23.58%), amoxicillin+clavulanic acid (21.70%), and chloramphenicol (12.26%); 16.98% of the isolates were ex-tended spectrum β-lactamase (ESBL) producers, and 76.41% were multidrug resistant (MDR). MDR Salmonella were significantly higher ( P < 0.01 ) in RMS (91.66%) followed by CBF (82.75%), whereas no MDR isolates were present in the isolates from hatcheries. The results indicated a higher prevalence of Salmonella and AMR for commonly used antibiotics in the complete broiler supply chain, especially RMS and CBF. Also, this study idicated that TTB enrichment followed by PCR and colony PCR was found to be rapid, specific and time-saving method.

scholarly journals Isolation and Identification of Biosurfactant Producing Bacteria from Slaughterhouse Wastewater

2021 ◽  
Vol 9 (1) ◽  
pp. 49
Author(s):  
Kristina Sinaga ◽  
M. Hasbi ◽  
Eko Purwanto
Keyword(s):  
Polymerase Chain Reaction ◽  
Polluted Area ◽  
Proteus Vulgaris ◽  
Chain Reaction ◽  
Isolation And Identification ◽  
Slaughterhouse Wastewater ◽  
Pcr Polymerase Chain Reaction ◽  
Emulsification Index ◽  
Polymerase Chain

The slaughterhouse wastewater is rich of oil and fat and it may inhabit by biosurfactant producing bacteria. Isolation of the bacteria is needed in order to find out a biosurfactant producing bacteria that can be used to maintain the oil and fat polluted area through bioremediation process. To isolate and identify the biosurfactant-producing bacteria, a study has been conducted on July to September 2020. The samples were obtained from slaughterhouse wastewater at Cipta Karya street Pekanbaru, Riau Province and samplings were conducted three times. The isolation was conducted by TSB (Tryptic Soy Broth)  and incubated for 24 hours. Then the bacterial samples were planted by TSA (Tryptic Soy Agar)  and isolated for 24 hours. Then, it was identified using biochemical and morphological tests were carried out to find out the types of bacteria. The Emulsification Index was then calculated by using TSB (Tryptic Soy Broth) and kerosene. Results shown that the emulsification index were Flavobacterium 53%, Agrobacterium 53%, Serratia 60%, Salmonella 52. 5%, Salmonella 52.4%, Proteus 65.7%, Clostridium 62% and Aeromonas 59.5%. As Proteus is shown the highest Emulsification Index, it was check using a PCR (Polymerase Chain Reaction) and it is proved that the species is Proteus vulgaris

scholarly journals EVALUATION OF THE EFFECTIVENESS OF COMMERCIALLY AVAILABLE DISINFECTANTS AGAINST SALMONELLAE ISOLATED FROM INTERNAL ORGANS OF DEAD CHICKENS

2012 ◽  
Vol 9 (1) ◽  
pp. 43-52 ◽  
Author(s):  
NA Rumi ◽  
MM Rahman ◽  
MR Akter ◽  
M Fakhruzzaman ◽  
MK Hossain
Keyword(s):  
Dead Layer ◽  
Salmonella Infection ◽  
Zone Of Inhibition ◽  
Internal Organs ◽  
Biochemical Methods ◽  
Agar Media ◽  
Layer Chickens

The effectiveness of commercially available disinfectants was evaluated against Salmonellae isolated from different internal organs of 52 numbers of dead layer chickens in the district of Dinajpur during the period from July 2009 to June 2010. Bacterio-biochemical methods were used to isolate and identify the Salmonella organisms from 154 samples of liver, spleen, heart and lungs of birds, of which 36 (23.38%) samples had Salmonella infection. Organ-wise prevalence of Salmonella infection showed highest prevalence in liver ( 34.62%), followed by spleen (23.087%) , heart (20.00%) and lowest in lungs (4.00%). Effectiveness of disinfectants was tested at different concentration on Salmonella culture on SS agar media and measured by zone of inhibition incubated for 24 hours. Highest average zone of inhibition was recorded with Desinkap® (12.50 ± 2.08), followed by GPC (8) TM ® (11.33 ± 1.53) , and more or less similar patterns with TH 4 ® ( 10.50 ± 0.71), Virocid ® ( 10.50 ± 0.71) and Lysol ® ( 10.00 ± 0.00). It may be concluded from these results that Desinkap® is the most effective disinfectant against Salmonellae as it contains multiple ingredients.DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11211 Bangl. J. Vet. Med. (2011). 9(1): 43-52 

scholarly journals Significance of appropriate techniques and media for isolation and identification of Ureaplasma urealyticum from clinical specimens

1978 ◽  
Vol 8 (4) ◽  
pp. 445-453
Author(s):  
R B Kundsin ◽  
A Parreno ◽  
S Poulin
Keyword(s):  
Epithelial Cells ◽  
Ureaplasma Urealyticum ◽  
Agar Medium ◽  
Clinical Material ◽  
Manganese Sulfate ◽  
Isolation And Identification ◽  
Urine Sediment ◽  
Clinical Specimens ◽  
Standard Textbook ◽  
Agar Media

Controversy over the association of Ureaplasma urealyticum with reproductive failure may be due to methods used to isolate the microorganism. U. urealyticum isolations from clinical material should be done simultaneously in broth and on Shepard's differential agar medium (A7) containing manganese sulfate. Urine sediments result in a 9% (P = 0.0002) higher rate of isolation than than cervical and urethral swabs. Primary isolations may not display standard textbook morphology. Isolated colonies may be present, but brown streaks in cervical mucus or a coalescent haze around epithelial cells in urine sediment may also be seen in areas of concentrated growth. The broth and agar media used, method of incubation, type of specimen, and method of storing specimens before culture are all factors which influence the recovery of U. urealyticum.

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