scholarly journals Molecular Identification and Analysis of Multi-Drug Resistant Klebsiella pneumonia

2018 ◽  
Vol 6 (3) ◽  
pp. 279-284
Author(s):  
Rajeswari Ramasamy ◽  
Subha Ashley Thanga Subramanian ◽  
R. Abinaya Rajagopal ◽  
Karthikadevi Muthusamy ◽  
Jesteena Johney ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Bacterial Infections ◽  
Genetic Material ◽  
Multidrug Resistant ◽  
Klebsiella Pneumonia ◽  
Drug Resistant ◽  
Remedial Measures ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Susceptibility Tests

Multidrug resistant Klebsiella pneumoniae was resistant to various antibiotics which are commonly used to treat   against the bacterial infections, and is now emerged as a great risk.  Antibiotic susceptibility tests were performed to determine the scope of drug resistance of the bacteria. Further studies regarding the responsible genetic material were performed by Polymerase Chain Reaction and RFLP techniques. The remedial measures for treating these bacteria were studied with the help of metabolites obtained from various strains.Int. J. Appl. Sci. Biotechnol. Vol 6(3): 279-284

scholarly journals Biofilm Forming Multi Drug Resistant Staphylococcus spp. among Patients with Conjunctivitis

2010 ◽  
Vol 59 (4) ◽  
pp. 233-239 ◽  
Author(s):  
K. MURUGAN ◽  
M. USHA ◽  
P. MALATHI ◽  
A. SALEH AL-SOHAIBANI ◽  
M. CHANDRASEKARAN
Keyword(s):  
Polymerase Chain Reaction ◽  
Biofilm Formation ◽  
Multidrug Resistant ◽  
Penicillin G ◽  
Drug Resistant ◽  
Chain Reaction ◽  
Antibiotic Resistance Profile ◽  
Polymerase Chain ◽  
Staphylococcus Spp ◽  
Close Genetic Relationship

Biofilm forming multidrug resistant Staphylococcus spp. are major reservoirs for transmission of ophthalmic infections. They were isolated from ocular patients suffering from conjunctivitis. In this study we analyzed biofilm forming ability, antibiotic resistance profile of the Staphylococcus spp. isolated from clinical ocular patients, and their phylogenetic relationship with other community MRSA. Sixty Staphylococcus spp. strains isolated from clinical subjects were evaluated for their ability to form biofilm and express biofilm encoding ica gene. Among them 93% were slime producers and 87% were slime positive. Staphylococcus aureus and S. epidermidis were dominant strains among the isolates obtained from ocular patients. The strains also exhibited a differential biofilm formation quantitatively. Antibiotic susceptibility of the strains tested with Penicillin G, Ciprofloxacin, Ofloxacin, Methicillin, Amikacin, and Gentamicin indicated that they were resistant to more than one antibiotic. The amplicon of ica gene of strong biofilm producing S. aureus strains, obtained by polymerase chain reaction, was sequenced and their close genetic relationship with community acquired MRSA was analyzed based on phylogenetic tree. Our results indicate that they are genetically close to other community acquired MRSA.

scholarly journals 484. DNA analysis with polymerase chain reaction (PCR) of bacterial infections

1992 ◽  
Vol 48 (8) ◽  
pp. 1567
Author(s):  
Takashi Arai ◽  
Hideaki Yamamoto ◽  
Satoshi Kobayashi ◽  
Akira Yamaguchi ◽  
Katsumi Ikei
Keyword(s):  
Polymerase Chain Reaction ◽  
Bacterial Infections ◽  
Dna Analysis ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Occurrence of virulent multidrug-resistantEnterococcus faecalisandEnterococcus faeciumin the pigs, farmers and farm environments in Malaysia

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5353 ◽  
Author(s):  
Shiang Chiet Tan ◽  
Chun Wie Chong ◽  
Cindy Shuan Ju Teh ◽  
Peck Toung Ooi ◽  
Kwai Lin Thong
Keyword(s):  
Polymerase Chain Reaction ◽  
Antibiotic Resistance ◽  
Environmental Factors ◽  
Environmental Samples ◽  
Virulence Genes ◽  
Multidrug Resistant ◽  
Peninsular Malaysia ◽  
Chain Reaction ◽  
Swine Farms ◽  
Polymerase Chain

BackgroundEnterococcus faecalisandEnterococcus faeciumare ubiquitous opportunistic pathogens found in the guts of humans and farmed animals. This study aimed to determine the occurrence, antimicrobial resistance, virulence, biofilm-forming ability and genotypes ofE. faecalisandE. faeciumfrom swine farms. Correlations between the genotypes, virulotypes, antibiotic resistance, and the environmental factors such as locality of farms and farm hygiene practice were explored.MethodsE. faecalisandE. faeciumstrains were isolated from the oral, rectal and fecal samples of 140 pigs; nasal, urine and fecal samples of 34 farmers working in the farms and 42 environmental samples collected from seven swine farms located in Peninsular Malaysia. Antibiotic susceptibility test was performed using the disk diffusion method, and the antibiotic resistance and virulence genes were detected by Polymerase Chain Reaction. Repetitive Extragenic Palindromic-Polymerase Chain Reaction and Pulsed-Field Gel Electrophoresis were performed to determine the clonality of the strains. Crosstab/Chi-square test and DistLM statistical analyses methods were used to determine the correlations between the genotypes, virulence factors, antibiotic resistance, and the environmental factors.ResultsA total of 211E. faecalisand 42E. faeciumwere recovered from 140 pigs, 34 farmers and 42 environmental samples collected from seven swine farms in Peninsular Malaysia. Ninety-eight percent of the strains were multidrug-resistant (resistant to chloramphenicol, tetracycline, ciprofloxacin and erythromycin). Fifty-two percent of the strains formed biofilms. Virulence genesefa, asaI, gelE,esp,cylandacegenes were detected. Virulence genesefaandasaI were most prevalent inE. faecalis(90%) andE. faecium(43%), respectively. Cluster analyses based on REP-PCR and PFGE showed the strains were genetically diverse. Overall, the strains isolated from pigs and farmers were distinct, except for three highly similar strains found in pigs and farmers. The strains were regional- and host-specific.DiscussionThis study revealed alarming high frequencies of multidrug-resistant enterococci in pigs and swine farmers. The presence of resistance and virulence genes and the ability to form biofilm further enhance the persistence and pathogenicity of the strains. Although the overall clonality of the strains were regionals and host-specific, strains with high similarity were found in different hosts. This study reiterates a need of a more stringent regulation to ensure the proper use of antibiotics in swine husbandry to reduce the wide spread of multidrug-resistant strains.

scholarly journals Epidemiology of epizootic lymphangitis of carthorses in northern Ethiopia using conventional diagnostic methods and nested polymerase chain reaction

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Birhanu Hadush ◽  
Molla Michaelay ◽  
Habtamu Taddele Menghistu ◽  
Nigus Abebe ◽  
Abreha Tesfaye Genzebu ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Examination ◽  
Nested Pcr ◽  
Control Measure ◽  
Genetic Material ◽  
Diagnostic Methods ◽  
Northern Ethiopia ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

Abstract Background Epizootic lymphangitis (EL), caused by Histoplasma capsulatum variety farciminosum (HCF) is a contagious, chronic disease of equines, characterized by development of nodular lesions in the lymph nodes, lymphatic vessels and skin. It is one of the most important diseases of equines in Ethiopia, causing significant economic loss, particularly in the livelihood of carthorse owners. To date there is neither effective diagnostic nor control measure implemented in the country. Furthermore, there is a shortage of data on the epidemiology of the disease in different regions of this country. The aim of this study was to investigate epidemiology of EL in northern Ethiopia, using the conventional methods as well as nested polymerase chain reaction (PCR). Results The presence of HCF genetic material was confirmed in 44% (84/191) of the carthorses. Subclinical infection was observed in 18.2% (22/121) of the apparently healthy carthorses. Considering the nested PCR as a gold standard, sensitivity and specificity of clinical examination were 74% and 92.5%, respectively, while the area under the ROC curve (AUR) was 0.83 (95% confidence interval, 0.77, 0.896). Moreover, a moderate (k = 0.675) agreement observed between the nested PCR and clinical examination. Conclusions This study demonstrated widespread occurrence of EL in northern Ethiopia, and the advantage of the nested PCR in detecting infection of HCF, even before the clinical symptoms became apparent.

Tea: An anti-bacterial agent against drug resistant bacteria

2021 ◽  
Vol 15 (10) ◽  
pp. 2506-2511
Author(s):  
Nayyab Sultan ◽  
Sabahat Javaid Butt ◽  
Wajeeha Mehak ◽  
Samreen Qureshi ◽  
Syed Hamza Abbas ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Salmonella Typhi ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Klebsiella Pneumonia ◽  
Drug Resistant ◽  
Antibiotic Resistant ◽  
Bacterial Agent ◽  
Drug Resistant Bacteria ◽  
Scientific World

Antibiotics have played a crucial role in the treatment of bacterial infections. Past few decades are marked with advancement of multidrug resistant (MDR) pathogens, which have endangered antibiotic’s therapeutic efficacy. Scientific world is now struggling with the crisis of MDR pathogens. This supreme matter demands careful attention or otherwise it would jeopardize clinical management of infectious diseases. Implication of alternative approaches can pave a new way in the treatment of these troublesome bacteria. Tea leaves are known to pose antibacterial activity against many pathogenic microorganisms. This review has summarized the antibacterial potential of tea leave’s extracts against resistant bacterial pathogens such as Staphylococcus aureus, Pseudomonas aeruginosa, Helicobacter pylori, Escherichia coli, Klebsiella pneumonia, Salmonella typhi, Acenitobacter spp, Campylobacter spp. Consumption of natural products such as tea may very well replace, minimize or obliterate this complicated situation. Keywords: Anti-bacterial, Tea, Camellia sinensis, Drug resistant bacteria, Antibiotic resistant bacteria, Synergism, Polyphenols.

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