Assessment of Crude Oil Degradation by Mixed Culture of Bacillus subtilis and Pseudomonas aeruginosa at Different Concentrations

2020 ◽  
Vol 9 (2) ◽  
pp. 33-50
Author(s):  
A.A. Faggo ◽  
A.H. Kawo ◽  
B.H. Gulumbe ◽  
U.J.J. Ijah
Keyword(s):  
Crude Oil ◽  
Mixed Culture ◽  
Control Sample ◽  
Gas Chromatography Mass Spectrometry ◽  
Oil Degradation ◽  
Pollution Levels ◽  
Degrading Bacteria ◽  
Crude Oil Degradation ◽  
Light Oil ◽  
Oil Concentration

Petroleum hydrocarbon (PHCs) contamination of soil, freshwater and air is of global concern. The aim of this study was to assess the extent of crude oil degradation by mixed bacterial culture of different crude oil concentrations using gas chromatography-mass spectrometry (GC-MS). Seven oil samples were collected from petroleum-contaminated fields in Kano state, Nigeria, and screened for crude oil utilizing bacteria. A control sample of soil from an ecological garden (control soil) was also analyzed. Crude oil-degrading bacteria were isolated, enumerated and identified using cultural, morphological and biochemical characteristics, and screened for their ability to utilize Bonny Light Oil as a source of carbon and energy. Bacteria with the highest potential to utilize crude oil were selected and subjected to bioremediation studies at three different pollution levels (5%, 10% and 15%) for 56 days. The residual crude oil was assessed using GC-MS. The results revealed that the mixed culture completely degraded eighteen components ranging from C10 to C25 at 5% crude oil concentration while only C8 to C11 and C8 to C9 were degraded at 10 and 15% respectively. The results of this study indicated the potential of B. subtilis and P. aeruginosa in bioremediation of crude oil contaminated soil.

scholarly journals Biodegradable Capability of the Indigenous Micrococcus sp. Oil Degrading Bacteria Isolated from Oil Contaminated Soil, Motor Workshop Area of Bahror, Alwar, Rajasthan, India.

2020 ◽  
Vol 4 (4) ◽  
pp. 1-4
Keyword(s):  
Crude Oil ◽  
Growth Conditions ◽  
Soil Samples ◽  
Oil Degradation ◽  
Environment Pollution ◽  
Physical Chemical ◽  
Degrading Bacteria ◽  
Crude Oil Degradation ◽  
Bacillus Spp ◽  
Biological Methods

Crude oil contamination is one of the important issue in the current environment pollution. Physical, chemical and biological methods are applied for bioremediation. Use of microorganisms is one of the most popular methods among them. In this experiment, soil samples were collected from the different motor workshop areas to isolate potential microbes capable of crude oil degradation. Isolation of the crude oil degrading bacteria was followed by enriching the microorganisms by providing suitable growth conditions. The microorganisms those were capable of degrading the crude oil were identified as Bacillus spp., Pseudoxanthomonas spp., Phenylobacterium spp. and Micrococcus spp. by morphological and biological methods. Among them, biodegradation capability of Micrococcus sp. was studied at different oil concentrations.

scholarly journals Biofilm-Mediated Enhanced Crude Oil Degradation by Newly Isolated Pseudomonas Species

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Debdeep Dasgupta ◽  
Ritabrata Ghosh ◽  
Tapas K. Sengupta
Keyword(s):  
Carbon Source ◽  
Crude Oil ◽  
Sole Carbon Source ◽  
River Estuary ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Oil Degradation ◽  
Topographic Analysis ◽  
Degrading Bacteria ◽  
Crude Oil Degradation

The bioavailability of organic contaminants to the degrading bacteria is a major limitation to efficient bioremediation of sites contaminated with hydrophobic pollutants. Such limitation of bioavailability can be overcome by steady-state biofilm-based reactor. The aim of this study was to examine the effect of such multicellular aggregation by naturally existing oil-degrading bacteria on crude oil degradation. Microorganisms, capable of utilizing crude oil as sole carbon source, were isolated from river, estuary and sea-water samples. Biochemical and 16S rDNA analysis of the best degraders of the three sources was found to belong to the Pseudomonas species. Interestingly, one of the isolates was found to be close to Pseudomonas otitidis family which is not reported yet as a degrader of crude oil. Biodegradation of crude oil was estimated by gas chromatography, and biofilm formation near oil-water interface was quantified by confocal laser scanning microscopy. Biofilm supported batches of the isolated Pseudomonas species were able to degrade crude oil much readily and extensively than the planktonic counterparts. Volumetric and topographic analysis revealed that biofilms formed in presence of crude oil accumulate higher biomass with greater thickness compared to the biofilms produced in presence of glucose as sole carbon source.

MICROBIAL COMMUNITY DURING BIOREMEDIATION EXPERIMENTAL ON OIL SPILL IN COASTAL OF PARI ISLAND

2011 ◽  
Vol 3 (1) ◽  
Author(s):  
Lies Indah Sutiknowati
Keyword(s):  
Oil Spill ◽  
Deoxyribonucleic Acid ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Gas Chromatography Mass Spectrometry ◽  
Oil Degradation ◽  
Sequence Determination ◽  
Blast Search ◽  
Degrading Bacteria ◽  
Gradient Gel Electrophoresis

There is an information how to identify hydrocarbon degrading bacteria for bioremediation of marine oil spill. We have Bioremediation treatment for degradation of oil spill on Pari island and need two kind of experiment there are tanks experiment (sampling 0 to 90 days) and semi enclosed system (sampling 0 to 150 days). Biostimulation with nutrients (N and P) was done to analyze biodegradation of hydrocarbon compounds. Experiment design using fertilizer Super IB and Linstar will stimulate bacteria can degrade oil, n-alkane, and alkane as poly aromatic hydrocarbon. The bacteria communities were monitored and analyzed by Denaturing Gradient Gel Electrophoresis (DGGE) and Clone Library; oil chemistry was analyzed by Gas Chromatography Mass Spectrometry (GCMS). DNA (deoxyribonucleic acid) was extracted from colonies of bacteria and sequence determination of the 16S rDNA was amplified by primers U515f and U1492r. Strains had been sequence and had similarity about 90-99% to their closest taxa by homology Blast search and few of them suspected as new species. The results showed that fertilizers gave a significant effect on alkane, PAH and oil degradation in tanks experiment but not in the field test. Dominant of the specific bacteria on this experiment were Alcanivorax, Marinobacter and Prosthecochloris. Keywords: Bioremediation, Biostimulation, DGGE, PAH, Pari Island

Methanogenic crude oil degradation induced by an exogenous microbial community and nutrient injections

2021 ◽  
Vol 201 ◽  
pp. 108458
Author(s):  
Konomi Suda ◽  
Masayuki Ikarashi ◽  
Hideyuki Tamaki ◽  
Satoshi Tamazawa ◽  
Susumu Sakata ◽  
...  
Keyword(s):  
Microbial Community ◽  
Crude Oil ◽  
Oil Degradation ◽  
Crude Oil Degradation

Comparison of surficial CO2 efflux to other measures of subsurface crude oil degradation

2014 ◽  
Vol 164 ◽  
pp. 275-284 ◽  
Author(s):  
Ean Warren ◽  
Natasha J. Sihota ◽  
Frances D. Hostettler ◽  
Barbara A. Bekins
Keyword(s):  
Crude Oil ◽  
Co2 Efflux ◽  
Oil Degradation ◽  
Crude Oil Degradation

scholarly journals MICROBIAL COMMUNITY DURING BIOREMEDIATION EXPERIMENTAL ON OIL SPILL IN COASTAL OF PARI ISLAND

2011 ◽  
Vol 3 (1) ◽  
Author(s):  
Lies Indah Sutiknowati
Keyword(s):  
Oil Spill ◽  
Deoxyribonucleic Acid ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Gas Chromatography Mass Spectrometry ◽  
Oil Degradation ◽  
Sequence Determination ◽  
Blast Search ◽  
Degrading Bacteria ◽  
Gradient Gel Electrophoresis

There is an information how to identify hydrocarbon degrading bacteria for bioremediation of marine oil spill. We have Bioremediation treatment for degradation of oil spill on Pari island and need two kind of experiment there are tanks experiment (sampling 0 to 90 days) and semi enclosed system (sampling 0 to 150 days). Biostimulation with nutrients (N and P) was done to analyze biodegradation of hydrocarbon compounds. Experiment design using fertilizer Super IB and Linstar will stimulate bacteria can degrade oil, n-alkane, and alkane as poly aromatic hydrocarbon. The bacteria communities were monitored and analyzed by Denaturing Gradient Gel Electrophoresis (DGGE) and Clone Library; oil chemistry was analyzed by Gas Chromatography Mass Spectrometry (GCMS). DNA (deoxyribonucleic acid) was extracted from colonies of bacteria and sequence determination of the 16S rDNA was amplified by primers U515f and U1492r. Strains had been sequence and had similarity about 90-99% to their closest taxa by homology Blast search and few of them suspected as new species. The results showed that fertilizers gave a significant effect on alkane, PAH and oil degradation in tanks experiment but not in the field test. Dominant of the specific bacteria on this experiment were Alcanivorax, Marinobacter and Prosthecochloris. Keywords: Bioremediation, Biostimulation, DGGE, PAH, Pari Island

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