scholarly journals Evaluation of maize genotypes against post flowering stalk rot under terai region of Nepal

2021 ◽  
Vol 4 (1) ◽  
pp. 239-247
Author(s):  
Subash Subedi ◽  
Saraswati Neupane ◽  
Keshab Babu Koirala ◽  
Lokendra Oli
Keyword(s):  
Hot Spot ◽  
Block Design ◽  
Grain Filling ◽  
Summer Season ◽  
Biotic Stresses ◽  
Stalk Rot ◽  
Maize Genotypes ◽  
Maize Varieties ◽  
Rot Disease ◽  
Serious Disease

The inadequate source of resistance materials in maize against major biotic stresses is one of the main reasons for considerable loss of grain yield in Nepal. Post flowering stalk rot disease caused by Fusarium moniliforme is a serious disease that exposes high incidence at grain filling stage of maize in terai region of Nepal during summer season. This study was done to evaluate level of resistance, or tolerance in selected genotypes against the post flowering stalk rot disease of maize. Accordingly, thirty maize genotypes were tested for maize stalk rot resistance during summer season of 2016 and 2017 at National Maize Research Program, Rampur (NMRP), Chitwan. The experiment was done under natural epiphytotic condition at hot spot of the disease by using Randomized Complete Block design with 2 replications for each treatment. The package of practices was followed as per national recommendation. The summer season of 2016 and 2017 were affable for post flowering stalk rot of maize at NMRP, Rampur. Out of 30 genotypes, most of the tested entries showed susceptible reaction during both the years; however, RML-95/RML-96, Across-9942/Across-9944, ZM-401, Rampur 34, RamS03F08 and TLBRS07F16 showed resistant reaction against the disease and might be useful for the development of post flowering stalk rot resistant maize varieties for terai region of Nepal.

scholarly journals Evaluation of white grain maize varieties for growth, yield and yield components

2021 ◽  
Vol 4 (1) ◽  
pp. 265-272
Author(s):  
Arjun Bastola ◽  
Ankit Soti ◽  
Utshav Pandey ◽  
Mausami Rana ◽  
Manoj Kandel ◽  
...  
Keyword(s):  
Plant Height ◽  
Block Design ◽  
Growth Yield ◽  
Research Field ◽  
Stay Green ◽  
Kernel Weights ◽  
Hill Region ◽  
Maize Genotypes ◽  
Maize Varieties ◽  
White Grain

Maize (Zea mays L.)  is one of the most commonly cultivated crop after rice in Nepal. The present study was done to evaluate and recommend the best performing white maize genotypes in mid hill region of Nepal. This study was conducted at research field of Kavre, Nepal during the rainy season of 2019. Five white maize genotypes were evaluated in randomized complete block design with four replications where Deuti used as standard check. Ear and plant height of plant, days to 50% silking and tasseling, count of leaf above and below main cob, total number of leaf, cob length, cob diameter, kernel rows per cob, kernels count per row, thousand kernels weight, shelling and sterility percentage, stay green and grain yield parameters were observed. Deuti and DMH-7314 had good stay green and husk cover rating. Plant height (282.6 cm) and ear height (162.4 cm) was more in HB-008. Number of kernels per row was more in HB-008 (36.5) and HB-007 (36.5) and thousand kernel weights was more in DMH-7314 (386.3 g) followed by Deuti (353.9 g). DMH-7314 was late in tasseling (86 days) and silking (89 days) but shelling percentage was lowest in DMH-7314 (70.8) than other varieties. Analysis of variance reveled that genotype HB-008 (9.70 t/ha) as compared to standard check Deuti (7.80 t/ha). Thus genotype HB-008 perform better in mid hill region of Kavre, Nepal.

scholarly journals Performance evaluation of quality protein maize genotypes across various maize production agro ecologies of Nepal

2015 ◽  
Vol 1 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Jiban Shrestha ◽  
Keshab B Koirala ◽  
Ram B. Katuwal ◽  
Narayan B Dhami ◽  
Bhanu B Pokhrel ◽  
...  
Keyword(s):  
Performance Evaluation ◽  
Block Design ◽  
Field Trials ◽  
Climatic Conditions ◽  
Summer Season ◽  
Quality Protein Maize ◽  
Maize Production ◽  
Maize Genotypes ◽  
Randomized Complete Block Design ◽  
Superior Genotypes

To identify superior quality protein maize genotypes for grain yield under different agro climatic conditions of terai and hill districts in Nepal, the coordinated varietal trials (CVT) were conducted at Dailekh, Doti, Salyan, Lumle and Pakhribas in 2013 and Salyan, Pakhribas and Kabre in 2014 during summer season and coordinated farmer’s field trials (CFFT) at Surkhet and Dailekh in 2013 and Salyan, Pakhribas and Khumaltar in 2014 during summer season. The experiment was carried out using randomized complete block design with three replications for CVT and CFFT. Across the locations and years the superior genotypes found under CVT were S01SIYQ, S01SIWQ-2 and Poshilo Makai-1 where as S99TLYQ-HG-AB, S99TLYQ-B and Poshilo Makai-1 were found superior genotypes under CFFT. The superior genotypes derived from CFFT will be promoted further for similar environments across the country.Journal of Maize Research and Development (2015) 1(1):21-27DOI: http://dx.doi.org/10.5281/zenodo.34282

scholarly journals Productivity of winter maize as affected by varieties and fertilizer levels

2020 ◽  
Vol 4 (1) ◽  
pp. 85-93
Author(s):  
Bishnu Hari Adhikary ◽  
Bandhu Raj Baral ◽  
Jiban Shrestha
Keyword(s):  
Grain Yield ◽  
Research Program ◽  
Block Design ◽  
Yield Production ◽  
Maize Genotypes ◽  
Maize Varieties ◽  
Randomized Complete Block Design ◽  
Nitrogen Phosphorus

Grain yield production of maize is greatly affected by varieties and fertilizer levels. This study was conducted to determine the effects of different rates of fertilizers (nitrogen, phosphorus, potassium fertilizers and farmyard manures) on grain yield and yield attributing traits of different maize varieties during winter seasons of 2009/10 and 2010/011 at the research farm of National Maize Research Program, Rampur, Chitwan, Nepal. Six levels of fertilizers [Control (Zero fertilizer), FYM @ 10 t/ha, FYM@ 10 t/ha plus 60:30 20 kg NPK/ha, FYM@ 10 t/ha plus 120: 60: 40 kg NPK/ha, FYM@ 10 t/ha plus 180: 90: 60 kg NPK/ha, and 120: 60: 40 kg NPK/ha] and four maize varieties (Rampur Composite, Manakamana-4, Across9942 × Across 9944 and S99TLYQ-B) were evaluated in randomized complete block design with three replications. The results showed that grain yield was non-significant for maize genotypes but the fertilizers levels were highly significant for grain yield. Rampur Composite produced the highest grain yield (5195 kg/ha), followed by Manakamana-4 (5074 kg/ha), Across9942 × Across9944 (5052 kg/ha) and S99TLYQ-B (4789 kg/ha) with the application of NPK 180: 90: 60 kg/ha plus FYM 10 t/ha. This information is useful in generating suitable fertilization packages for obtaining higher grain yield of maize varieties.

scholarly journals Raman-Based Diagnostics of Stalk Rot Disease of Maize Caused by Colletotrichum graminicola

2021 ◽  
Vol 12 ◽  
Author(s):  
Charles Farber ◽  
John S. Bennett ◽  
Tianyi Dou ◽  
Yousef Abugalyon ◽  
Dillon Humpal ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Spectroscopic Method ◽  
High Accuracy ◽  
Diagnostic Methods ◽  
Colletotrichum Graminicola ◽  
Stalk Rot ◽  
Non Invasive ◽  
Associated Lesions ◽  
Maize Genotypes ◽  
Rot Disease

Stalk rot caused by Colletotrichum graminicola is a disease of worldwide importance. Stalk rot is difficult to detect at the early stages of infection because the fungus colonizes the tissues inside the maize stem. Current diagnostic methods are time-consuming, laborious, and destructive to the stem tissue. We utilized Raman spectroscopy to follow the development of stalk rot in three different maize genotypes grown either in the field or the greenhouse. We then used the acquired spectra to calibrate statistical models to differentiate amongst the different disease timepoints and the genotypes themselves. This non-invasive spectroscopic method enabled high-accuracy identification of stalk rot based on both stalk and leaf spectra. We additionally found that leaf spectra were favorable for identifying maize by genotype. Finally, we identified Raman bands that showed correlation with the sizes of stalk rot-associated lesions in the stems. We demonstrated that Raman spectroscopy is a viable tool for detection of stalk rot disease, as well as potent for the differentiation of maize genotypes.

Association among Agro-morphological Traits by Correlations and Path in Selection of Maize Genotypes

2019 ◽  
pp. 1-12
Author(s):  
Richardson Sales Rocha ◽  
Maxwel Rodrigues Nascimento ◽  
José Tiago Barroso Chagas ◽  
Rafael Nunes De Almeida ◽  
Paulo Ricardo Dos Santos ◽  
...  
Keyword(s):  
Plant Height ◽  
Morphological Traits ◽  
Smallholder Farmers ◽  
Block Design ◽  
Yield Increase ◽  
Maize Populations ◽  
Maize Genotypes ◽  
Maize Varieties ◽  
Plant Stand

In the State of Espírito Santo (Brazil), family farmers have grown a number of maize varieties for decades, consisting of open-pollinated populations with valuable importance for the livelihood of smallholder farmers. The study aimed to analyze the cause and effect of associations between agro-morphological traits for increasing yields in maize populations cultivated. A total of 16 maize accessions of in vivo conservation works in eight different locations were evaluated, in a randomized complete block design with three replicates, totaling 48 experimental units. Genetic parameters, genotypic, phenotypic, and environmental correlation were estimated, and a path analysis was conducted. In general, accessions presented enough genetic variability, showing significant differences for all traits analyzed statistically (P≤0.05). The first cob insertion height with plant height and final plant stand variables presented heritability (h2) higher than 80%, suggesting that superior genotypes could be determined. Highest estimates of phenotypic correlations (rp) were found between the first cob insertion height with plant height and total number of cobs with number of cobs per plant (0.85). For estimates of genotypic correlations (rg), the highest were between (i) the first cob insertion height and plant height; (ii) number of grains per row and number of cobs per plant; (iii) total number of cobs and final plant stand. Maize final plant stand and one thousand seed weight were observed to be traits that could be determinant in grain yield increase.

scholarly journals Characterization and Molecular Mapping of Two Novel Genes Resistant to Pythium Stalk Rot in Maize

2019 ◽  
Vol 109 (5) ◽  
pp. 804-809
Author(s):  
Canxing Duan ◽  
Fengjing Song ◽  
Suli Sun ◽  
Cheng Guo ◽  
Zhendong Zhu ◽  
...  
Keyword(s):  
Yield Loss ◽  
Molecular Mapping ◽  
Physical Distance ◽  
Genetic Distances ◽  
Target Region ◽  
Stalk Rot ◽  
Maize Germplasm ◽  
Maize Varieties ◽  
Serious Disease ◽  
Dominant Genes

Pythium stalk rot caused by Pythium inflatum is becoming a more and more serious disease in maize, and it has caused severe yield loss in China in recent years. Deployment of resistant maize varieties is the most effective way to control this disease. Searching for the resistant maize germplasm and identifying the resistance genes are the vital processes in the breeding program. The maize inbred line X178 previously showed high resistance to Pythium stalk rot. Thus, this study aimed to reveal the gene(s) resistance to Pythium stalk rot in X178 by resistance inheritance analysis using the derived F2 and F2:3 genetic populations. The results showed that two independently inherited dominant genes, designated RpiX178-1 and RpiX178-2, carried by X178 are responsible for its resistance relative to the susceptible parent Ye107; they are located on regions of maize chromosome (chr.) 1 bin 1.09 and chr. 4 bin 4.08, respectively, and flanked by markers umc2047 and bnlg1671 as well as bnlg1444 and umc1313, respectively, by linkage analysis. Subsequently, RpiX178-1 was finely mapped between markers SSRZ8 and IDP2347, with genetic distances of 0.6 and 1.1 cM, respectively, and the physical distance of the target region was about 700 kb. RpiX178-2 was also further located between markers bnlg1444 and umc2041, with a genetic distance of 2.4 cM. Moreover, we confirmed that the two genes RpiX178-1 and RpiX178-2 were newly identified and different from those genes known on chrs. 1 and 4 according to results of allelism testing. Herein, we newly identified two genes resistant to P. inflatum, which provided important genetic information for resistance to Pythium stalk rot in maize.

scholarly journals Environmental impact and genetic expressions of new drought tolerant maize genotypes in derived savannah agro-ecology

2021 ◽  
Vol 13 (1) ◽  
pp. 10691
Author(s):  
Paul INYANG ◽  
Chikezie O. ENE ◽  
Ankrumah EMMANUEL ◽  
Uchechukwu P. CHUKWUDI ◽  
Ugochukwu N. IKEOGU
Keyword(s):  
Food Security ◽  
Block Design ◽  
Sub Saharan Africa ◽  
International Institute ◽  
Improvement Program ◽  
Yield Data ◽  
Drought Tolerant ◽  
Maize Genotypes ◽  
Maize Varieties ◽  
Sub Saharan

Reduced water resources in sub-Saharan Africa will not only pose threat to the livelihood of poor resource farmers, but also food security in the region. Drought tolerant (DT) maize varieties hold promise to reducing poor resourced farmers’ vulnerability and improve food security in sub-Saharan Africa. Ten maize genotypes obtained from the International Institute of Tropical Agriculture (IITA), were evaluated in 2015 and 2016 using a randomized complete block design experiment with three replications to estimate their genetic variability and predict their genetic advances in the derived savannah agro-ecology. Growth, phenological and yield data were collected from 10 middle row plants. Genetic advance, genotypic, phenotypic and environmental coefficients of variations and their variances were estimated. Principal component and hierarchical cluster analyses were also performed. The dendrogram showed that at 80% dissimilarity point, the genotypes were grouped into clusters A, B and C in both years. The first two principal components explained 91.8% and 93.3% of the total variation in 2015 and 2016, respectively. Number of grains cob-1, plant height and number of days to physiological maturity were consistent in explaining the variations observed in the maize population. Heritability estimates in broad sense ranged from 1.35% for number of leaves to 87.43% for grain yield per hectare. The genetic parameters studied showed significant variations among the growth, phenological and yield data collected that warrants selection and maize improvement program using the DT maize inbred lines in derived savannah agro-ecology.

scholarly journals Studies on the Fusarium stalk rot infection of the maize genotypes using the Findex percentage and a computerised image analysis program

2011 ◽  
pp. 45-51
Author(s):  
Csaba Szőke ◽  
István Virág ◽  
Donát Magyar ◽  
Ferenc Rácz ◽  
Csaba L. Marton
Keyword(s):  
Image Analysis ◽  
Natural Infection ◽  
Grain Filling ◽  
Inbred Lines ◽  
Stalk Rot ◽  
Maize Genotypes ◽  
Accurate Picture ◽  
Computerised Image Analysis ◽  
Continental Climate ◽  
Plot Design

In a continental climate, the pathogens causing the most serious problems are species belonging to the Fusarium genus. When the pathogen attacks the stalk, the plant dies earlier, reducing grain filling and resulting in small, light ears. In addition, the stalks break or lodge, resulting in further yield losses from ears that cannot be harvested. During the three years of the experiment, 14 inbred lines were examined. The genotypes were sown in a two-factor split-plot design with four replications, with the genotypes in the main plots and four treatments in the subplots: two Fusarium graminearum isolates (1. FG36, 2. FGH4), 3. sterile kernels, 4. untreated control. The results experiments showed significant differences between the genotypes for resistance to fusarium stalk rot. Among the inbred lines the best resistance to fusarium stalk rot was exhibited by P06 and P07, both of which were related to ISSS. The precision and sensitivity of disease evaluations carried out visually and using image analysis software were compared in the experiment, and with two exceptions the CV values were lower for the image analysis. As the CV for measurements can be considered as a relative error, it can be stated that image analysis is the more precise of the two methods, so this technique gives a more accurate picture of the extent of stalk rot. The extent of stalk rot developing in response to natural infection is extremely environment-dependent, so the use of artificial inoculation is recommended for selection trials. 

scholarly journals Use of Fertilizers and Lime for Enhancing Productivity of Maize Genotypes in Western Hill of Nepal

2014 ◽  
Vol 8 ◽  
pp. 42-49
Author(s):  
Bishnu H. Adhikary ◽  
Krishna B. Karki
Keyword(s):  
Experimental Study ◽  
Plant Growth ◽  
Grain Yield ◽  
Summer Season ◽  
Grain Production ◽  
Maize Productivity ◽  
Maize Genotypes ◽  
Maize Varieties ◽  
Maize Variety ◽  
Yield Result

Farmers' field experiment was conducted at Outreach sites in Tanahun and Palpa districts of Western Nepal with the objectives of evaluating the effects of fertilizers and agricultural lime on grain production of the local and improved (Manakamana-1) maize varieties during summer season of year 2003 and 2004. Farmer's practice (without fertilizers) and with   fertilizer 60: 30: 30 kg (N: P2O5: K2O) per hectare with or without agricultural lime (4 t ha-1), were the treatments in experimental study that was conducted in factorial RCB design with four farmers as replications. The results revealed that response of fertilizers and agricultural lime was observed significant plant growth and kernels production including varieties in both sites of Palpa and Tanahun districts. The highest grain yield of 5.1 t ha-1 was produced by the local variety when the crop was supplied with 60:30:30 kg N, P2O5 and K2O ha-1 along with 4 t ha-1 of agricultural lime in Tanahun. Manakamana-1 variety produced the maximum grain yield (4.45 t ha-1) with the same level of fertilization. The two district mean grain yield result indicated that the highest grain yield of 3.9 t ha-1 was produced when the crop was supplied with 60: 30: 30 kg N, P2O5 and K2O ha-1 with agri-lime. Hence it is suggested that to increase maize productivity in the hills irrespective of the maize variety application of fertilizers (60: 30: 30) with agricultural lime (4 t ha-1) is needed.Nepal Agric. Res. J. Vol. 8, 2007, pp. 42-49DOI: http://dx.doi.org/10.3126/narj.v8i0.11578

scholarly journals Breeding Resistance for Post Flowering Stalk rot (Macrophomina phaseolina) in Maize Identification of Resistance against Post Flowering Stalk Rot (Macrophomina phaseolina) in Maize

2021 ◽  
pp. 44-55
Author(s):  
M. Banoth ◽  
K. Prabhavathi ◽  
D. Bhadru ◽  
B. Mallaiah
Keyword(s):  
Rating Scale ◽  
Block Design ◽  
Macrophomina Phaseolina ◽  
Yield Reduction ◽  
Stalk Rot ◽  
Randomized Block Design ◽  
Maize Genotypes ◽  
Additive Component ◽  
High Yields

Maize is one of the most important staple food crops in the World. However, the yields of maize have been affected by various fungal infestations. Post flowering stalk rot is one of the devastating diseases and so, we planned our study to order to identify suitable resistance maize genotypes against post flowering stalk rot (PFSR) complex caused by Macrophomina phaseolina through in-vivo screening and toothpick method for creating artificial epiphytotics. A total of 20 maize inbreds were screened and crossed in Line × Tester mating design (15 × 5) during Kharif 2019, Six resistant inbred lines were identified and generated the 75 F1s (SCHs) at MRC, ARI, Rajendranagar, Hyderabad. All these 20 parents and 75 F1s along with three checks were evaluated by raising the crop in disease sick plot accompanied by toothpick inoculation during Rabi, 2019-20, in a Randomized Block Design with two replications. The field screening of maize genotypes by the standard toothpick method which needs about 40 days for expression of plant drying symptoms due to PFSR and data are possible to record only at the time of crop harvesting using 1-9 rating scale of PFSR for scoring disease severity in-vivo condition by splitting the stem of each plant. As a result, most of the genotypes were exhibited disease reaction varying from resistant (score 2) to moderately resistant (score 5) against M. phaseolina. While studying the genetics of PFSR, we found that interaction of lines and testers were proportionally contributed towards resistant, and degree of dominance is preferably non-additive gene action, it shows that the magnitude of dominance was higher than additive effect indicating that PFSR resistance is largely governed by dominance effect i.e., non additive component is not fixable for resistance. It is also found that the resistant genotypes also exhibited highest significant positive heterosis and combining ability effects (GCA and SCA). A considerable yield reduction in grain yield (10.5 to 28.3%) over checks was observed in susceptible lines. Most of the genotypes were found resistant as the reduction in yield is low. Hybrids developed using such lines exhibited high yields which are promoted for extensive testing to know their stability before release as commercial hybrids.

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