Drug sensitivity pattern of microorganisms of infected wounds at TUTH

2007 ◽  
Vol 28 (1) ◽  
Author(s):  
A. Singh ◽  
P. Vaidya ◽  
N.R. Tuladhar ◽  
S. Maharjan
Keyword(s):  
Drug Sensitivity ◽  
Sensitivity Pattern ◽  
Infected Wounds

scholarly journals Drug sensitivity pattern of Escherichia coli isolated from samples of different biological and environmental sources

1970 ◽  
Vol 6 (1) ◽  
pp. 13-18 ◽  
Author(s):  
MA Zinnah ◽  
MH Haque ◽  
MT Islam ◽  
MT Hossain ◽  
MR Bari ◽  
...  
Keyword(s):  
Drug Sensitivity ◽  
Clinical Laboratory ◽  
Intermediate Phase ◽  
Sensitivity Test ◽  
Diffusion Method ◽  
National Committee ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Human Faces

A total of 100 different E. coli isolates collected from 10 different biological and environmental sources (10 isolates from each source) such as human faces, human urine, cattle, sheep, goat, chicken, duck, pigeon, drain sewage and soil were used for in-vitro drug sensitivity test in the Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh during the period from January to May 2007. Ten different drugs such as Gentamicin (GM), Azithromycin (AZM), Erythromycin (E), Levofloxacin (LVX), Ciprofloxacin (CIP), Tetracycline (TE), Amoxicillin (A), Ampicillin (AP), Nalidixic acid (NA) and Metronidazole (MET) were used in this study. Sensitivity test was carried out by the Kirby-Bauer disc diffusion method as per recommendation of National Committee for Clinical Laboratory Standards and efficacy of a drug was determined by measuring the diameter of the zone of inhibition that results from diffusion of the agent in to the medium surrounding the disc. A high of 80% and 78% E. coli isolates collectively from all the selected sources were sensitive to LVX and CIP respectively, followed by GM (46%), AZM (45%), TE (30%), AP (29%), E (19%), NA (18%) and A (15%). No isolate was sensitive to MET (0%). Incase of resistance, 96% isolates were resistant to MET, followed by A (72%), E (69%), NA (67%), TE (60%), AP (59%), AZM (33%) and GM (32%), CIP (8%) and LVX (5%). A number of isolates showed intermediate reaction to GM (22%), AZM (22%), LVX (15%), NA (15%), CIP (14%), A(13%), AP (12%), E (12%), TE (10%) and MET (4%). This may be an intermediate phase for the conversion of E. coli isolates from sensitive to resistant form. From the research it may be concluded that E. coli infection of different animals and birds and also of human being may be treated effectively with LVX and CIP followed by GM and AZM. Key words: E. coli isolates, levofloxacin, ciprofloxacin, efficacy, resistance DOI = 10.3329/bjvm.v6i1.1332 Bangl. J. Vet. Med. (2008). 6 (1): 13-18

scholarly journals Study of Antibiotics Sensitivity Pattern And Molecular Characterization of Staphylococcus Aureus Isolated From Human And Animal Pyogenic Cases

2021 ◽  
Author(s):  
Jayshree Singh ◽  
Amit Kumar ◽  
Sharad K. Yadav ◽  
Ritika Yadav ◽  
Vinod K. Singh
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Characterization ◽  
Drug Sensitivity ◽  
Methicillin Resistance ◽  
Phenotypic Characterization ◽  
Resistance Pattern ◽  
Sensitivity Pattern ◽  
Drug Resistance Pattern ◽  
Animal Isolates

Abstract Staphylococcus aureus has been described as the most common cause of human and animal diseases and has emerged as superbug due to multidrug resistance. Considering these, a total of 175 samples were collected from pyogenic cases of humans (75) and animals (100), to establish the drug resistance pattern and also for molecular characterization of human and animal isolates. Thermonuclease (nuc) gene amplification was used to confirm all presumptive S. aureus isolates and then antibiotic sensitivity and slide coagulase tests were used for phenotypic characterization of isolates. Following that, all of the isolates were subjected to PCR amplification to detect the existence of the methicillin resistance (mecA) and coagulase (coa) genes. Lastly, typing was done by using the Randomly Amplified Polymorphic DNA-PCR. The overall prevalence of S. aureus in human and animal samples was found to be 39.4%. Drug sensitivity revealed the highest resistance against the β-lactam antibiotics such as ampicillin (94.8%) and penicillin (90.6%), followed by cephalosporin (cefixime-67.7%) and quinolone (ciprofloxacin-52.1%) group of drugs. The drug sensitivity was the highest against antibiotics like chloramphenicol (95%) followed by gentamicin (90%). Among the 69 S. aureus isolates, the overall presence of MRSA was 40.5% (27.5% and 50% in human and animal isolates respectively). Total 33 isolates exhibited coa genes amplification of more than one amplicons and variable in size of 250, 450, 800, and 1100 bp. The RAPD typing revealed amplification of 5 and 6 different band patterns in humans and animals, respectively, with two common patterns suggesting a common phylogenetic profile.

scholarly journals Tuberculous Meningitis: The Microbiological Laboratory Diagnosis and Its Drug Sensitivity Patterns

2019 ◽  
Vol 3 (2) ◽  
pp. 35
Author(s):  
Titiek Sulistyowati ◽  
Deby Kusumaningrum ◽  
Eko Budi Koendhori ◽  
Ni Made Mertaniasih
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Tuberculous Meningitis ◽  
Drug Sensitivity ◽  
Mycobacterium Tuberculosis Complex ◽  
Laboratory Diagnosis ◽  
Clinical Microbiology Laboratory ◽  
Tuberculosis Complex ◽  
First Line ◽  
Sensitivity Pattern ◽  
Microbiological Laboratory

Background: Tuberculosis continues one of the major challenges to global health. Mycobacterium tuberculosis complex can affect any organ other than the lung parenchyma, include central nervous system. The mortality rate of tuberculous meningitis (TBM) are high worldwide with up to half of survivors suffering irreversible sequelae. Diagnosis of TBM is difficult due to paucibacillary, various clinical manifestation, and invasive procedure to appropriate specimens. Objective: The objectiveis to study the positivity rate of microbiological laboratory diagnosis and its drug sensitivity patterns of TBM patients in Dr. Soetomo Hospital Surabaya during October 2015 until September 2016. Methods: Specimens were cerebrospinal fluids. Identification and drug anti TB sensitivity test were done by BACTEC MGIT 960 system in Clinical Microbiology Laboratory Dr. Soetomo Hospital Surabaya. Result: Most patients with TBM were women (54.29%). Based on age groups, most dominant was adult population (65.71%). Proportion percentage of positive M. tuberculosis complex among 180 specimens were 19.44%. First line anti TB drug sensitivity pattern of 35 isolates were 1 monoresistant, 1 poly-resistant, no multiple drug resistant (MDR), and 33 pan-susceptible. Conclusion: Positivity rate of Mycobacterium tuberculosis complex laboratory diagnosis from TBM suspect patients were low. There was no MDR TB in this study, but mono-resistant and poly-resistant. Microbiological diagnosis was important to give information of active disease and drug sensitivity pattern. Resistance to first line anti TB drugs is alarming to properly manage TBM patients.

scholarly journals Socio-demographic Profile and Drug Sensitivity Pattern of Suspected Drug Resistant Tuberculosis among Patients in Regional Tuberculosis Reference Laboratory (R.T.R.L) of a Tertiary Hospital

2017 ◽  
Vol 18 (2) ◽  
pp. 62-67 ◽  
Author(s):  
Ummay Fatema Khatun ◽  
Robed Amin ◽  
Muna Islam ◽  
Abdur Rob ◽  
Abdur Rahim
Keyword(s):  
Drug Resistance ◽  
Drug Sensitivity ◽  
Reference Laboratory ◽  
Drug Resistant ◽  
Suspected Drug ◽  
Drug Resistant Tuberculosis ◽  
Demographic Profile ◽  
Resistant Tuberculosis ◽  
Sensitivity Pattern ◽  
Mdr Tb

Background: Drug resistant tuberculosis has been reported in all regions of the world. In this study we address the socio-demographic profile and drug sensitivity pattern as well as prevalence of drug resistance tuberculosis in a tertiary center (regional TB reference laboratory) in Bangladesh.Method: This Study was carried out in R.T.R.L. (Regional TB Reference Laboratory) in 250 bedded Chittagong General Hospital. Patients who were referred to R.T.R.L during the period July 2012 to July 2013 were included in the study. Total 100 patients with suspected drug resistant tuberculosis (TB) who had any one of 9 criteria of NTP (National Tuberculosis Control Programme) were selected consecutively. Gene xpert MTB/RIF (Rifampicin resistance) test for all sputum positive cases were performed. Sputum sample of Patients with positive microscopy for AFB or positive Xpert/MTB was sent for culture. The samples with positive sputum culture were sent for drug sensitivity test for 1st line anti- tubercular drug.Result: Among 100 patients 78 were male and 22 were female, majority of the patients (64) were between 15-45 years with poor socio economic condition (73%) and primarily educated. Analysis of our Study result showed that 18% of patients were mono-drug resistant. Among them 13% showed resistance to isoniazid (INH), 4% to streptomycin and only 2% to rifampicin. No patient was found resistant to pyrazinamide (PNZ) and 38% of patient with suspected drug – resistant TB was found to have no drug resistance. 18% of patient had multidrug resistant tuberculosis (MDR-TB) among which 56% were relapse cases (48% after cat -I, 8% after cat II), 24% were non – converter of cat –I, 12% belonged to failure of cat –I, 3% failure of cat –II, 2% return after default and others. 1% of patient had history of contact with MDR –TB patient.Conclusion: Drug-resistance tuberculosis especially MDR-TB, was higher in patients with previously incomplete anti-tuberculosis treatment. A high level of drug resistance among the re-treatment TB patients poses a threat of transmission of resistant strains to susceptible persons in the community. For this reason proper counseling of patients and attention towards the completion of the anti-TB treatment are needed.J MEDICINE July 2017; 18 (2) : 62-67

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