scholarly journals The role of electron microscopy in kidney lesions: A review of its diagnostic importance

2013 ◽  
Vol 3 (5) ◽  
pp. 411-415
Author(s):  
AD Pant ◽  
K Solez
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Renal Biopsy ◽  
Renal Pathology ◽  
Malignant Diseases ◽  
The World ◽  
Kidney Lesions ◽  
Diagnostic Importance

Electron microscopy is a technology which was at one time widely used for renal as well as non-renal benign and malignant diseases, but its use has been rapidly declining as hospitals all over the world cut down on expenses. This leaves the renal pathologist with only light microscopy and immunofl uorescence at his disposal to diagnose diseases. Few studies have stated the importance of electron microscopy in routine renal biopsy reporting. We look at different cases where electron microscopy has been helpful in diagnosis and review the literature to assess the role this investigative modality still has to play in modern renal pathology. Journal of Pathology of Nepal (2013) Vol. 3, No.1, Issue 5, 411-415 DOI: http://dx.doi.org/10.3126/jpn.v3i5.7871

Oncocytoma of the larynx

1990 ◽  
Vol 104 (4) ◽  
pp. 346-349 ◽  
Author(s):  
Anthony C. Robinson ◽  
Antonios Kaberos ◽  
Phillip M. Cox ◽  
Michael P. Stearns
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
World Literature ◽  
Histopathological Diagnosis ◽  
True Vocal Cord ◽  
Accurate Definition ◽  
Thyroid Glands ◽  
The World ◽  
Adequate Surgery ◽  
Definition Of

AbstractA case of oncocytoma of the larynx is presented. These lesions are rarely found in the larynx, and have been more commonly described in the salivary and thyroid glands. The histopathological diagnosis is often difficult and the light microscopy can be unreliable for oncocytic lesions. For a more accurate definition of these tumours, the use of histochemical stains together with electron microscopy are advocated. With adequate surgery, the prognosis is excellent, and tumours are unlikely to recur. This paper discusses the management of a patient with a benign oncocytoma of the true vocal cord, and presents a review of the world literature.

Role of EM in interface science and engineering

1992 ◽  
Vol 50 (1) ◽  
pp. 208-209
Author(s):  
Gareth Thomas
Keyword(s):  
Electron Microscopy ◽  
Phase Contrast ◽  
Displacement Vector ◽  
The Other ◽  
Science And Engineering ◽  
Cross Sectional ◽  
Important Group ◽  
The World ◽  
Interface Science

The world of materials is a world of interfaces. Indeed many technologically significant materials have properties both physical and mechanical which are determined by the structure, composition, and bonding of the interfaces within these materials. Thus, electron microscopy and microanalysis, with its high resolution and specificity of information, is one of the key methods needed for characterization. Imaging can be done by amplitude contrast but is limited by the factor g • R (where R is a displacement vector), or resolution in phase contrast, and in today's modern instruments atomic arrangements can be imaged directly, both in plan and cross-sectional views. Beautiful examples are now being published. However so far, few developments to utilize this information for materials design have been forthcoming. On the other hand, interface or intergranular phases are very important in many metallurgical and ceramic systems. In fact many materials are composites of one kind or another and composites involving intergranular phases are an important group of such materials.

scholarly journals Formaldehyde as a Fixative for Light and Electron Microscopy

2000 ◽  
Vol 8 (5) ◽  
pp. 30-31
Author(s):  
Freida L. Carson
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Light And Electron Microscopy ◽  
Parallel Study ◽  
Electron Microscopic ◽  
Dual Purpose ◽  
Ultrastructural Morphology ◽  
The World ◽  
Selection Of

Since Blum discovered its hardening properties in 1893, formaldehyde has become the most widely used fixative in the world for specimens to be examined by light microscopy. However, since most commercial preparations of formaldehyde contain methanol, a protein precipitant, formaldehyde has been considered an unsatisfactory fixative for tissues to be examined by electron microscopy. In 1973, Carson et al., described a parallel study comparing the electron microscopic results of fixation with paraformaidehyde vs. formaldehyde. They found that there was no difference in the preservation of ultrastructural morphology provided that the buffer systems were identical. In 1976, McDowell and Trump described a fixative combining commercial formaldehyde and glutaraldehyde (4CF-1G). Both of these fixatives are dual purpose fixatives and preclude the selection of tissue for electron microscopy prior to fixation. They can both be prepared in large quantities and used for routine surgical specimens. The fixative containing formaldehyde alone does not need to be refrigerated and is stable for months; whereas, the formaldehyde-glutaraldehyde mixture should be refrigerated.

scholarly journals Role of Electron Microscopy in Early Detection of Altered Epithelium During Experimental Oral Carcinogenesis

2019 ◽  
Vol 25 (6) ◽  
pp. 1367-1375
Author(s):  
Sharada Sawant ◽  
Harsh Dongre ◽  
Deepak Kanojia ◽  
Sayli Jamghare ◽  
Anita Borges ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Early Detection ◽  
Light Microscopy ◽  
Cancer Progression ◽  
High Resolution Electron Microscopy ◽  
Cellular Adhesion ◽  
Significant Loss ◽  
Resolution Electron ◽  
Rat Tongue

AbstractEarly detection of altered epithelium can help in controlling the further progression by timely intervention. Alterations in cellular adhesion are one of the hallmarks of cancer progression, which can be detected at the intracellular level using high-resolution electron microscopy. This study aimed to evaluate the role of electron microscopy in the establishment of ultrastructural markers for early detection of altered epithelium using tissues from 4-Nitroquinoline-1-Oxide (4NQO) induced rat tongue carcinogenesis. Our previous study using light microscopy displayed no histopathological alterations in 4NQO treated tissues until 40 days of treatment, while dysplasia, papilloma and carcinoma were detected at 80/120, 160 and 200 days, respectively. However, electron microscopy detected alterations such as detachment of desmosomes from cell membranes and their clustering in the cytoplasm, increased tonofilaments, keratohyaline granules and thickened corneum in 40 days treated corresponding tissues. These alterations are apparent with hyperkeratosis/hyperplasia but remained undetected using light microscopy. Further, in dysplasia, papilloma and carcinoma, gradual and significant loss of desmosomes, leading to the significant widening of intercellular spaces, was observed using iTEM software. These parameters may serve as indicators for progression of oral cancer. Our results highlight the importance of electron microscopy in the early detection of subcellular changes in the altered epithelium.

scholarly journals Assessing morphological congruence in Dinobryon species and their stomatocysts, including a newly established Dinobryon pediforme–stomatocyst connection

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jolanta Piątek ◽  
Joanna Lenarczyk ◽  
Marcin Piątek
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Phylogenetic Relationships ◽  
Morphological Characters ◽  
Marine Waters ◽  
Resting Stages ◽  
The World ◽  
Scanning Electron ◽  
Sem Analyses

Abstract The chrysophyte genus Dinobryon Ehrenberg consists of 44 taxa, which occur in freshwaters, rarely marine waters, mostly in temperate regions of the world. The taxa of Dinobryon produce characteristic solitary or dendroid colonies and resting stages called stomatocysts. Only 20 Dinobryon taxa have information on produced stomatocysts and only four stomatocysts are reliably linked with vegetative stages using modern identification standards employing scanning electron microscopy (SEM) analyses. In this study, an encysted material of Dinobryon pediforme (Lemmermann) Steinecke was collected in two lakes in contrasting regions of Poland. Light microscopy (LM) and scanning electron microscopy (SEM) analyses revealed that Dinobryon pediforme produces stomatocyst #61, Piątek J. that is described here as new morphotype following the International Statospore Working Group (ISWG) guidelines. This raises to five the number of reliable links between vegetative stages of Dinobryon species and corresponding stomatocysts. Phenotypic similarities between Dinobryon species and their stomatocysts, analysed for five reliably established links, showed no relationships in size and shape between loricas and stomatocysts belonging to the same species. The morphological characters of loricas and stomatocysts mapped onto the phylogenetic tree of the five Dinobryon species revealed only little congruence between their morphology and phylogenetic relationships.

scholarly journals Formaldehyde as a Fixative for Light and Electron Microscopy

2000 ◽  
Vol 8 (3) ◽  
pp. 34-35
Author(s):  
Freida L. Carson
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Light And Electron Microscopy ◽  
Parallel Study ◽  
Electron Microscopic ◽  
Dual Purpose ◽  
Ultrastructural Morphology ◽  
The World ◽  
Selection Of

Since Blum discovered its hardening properties in 1893, formaldehyde has become the most widely used fixative in the world for specimens to be examined by light microscopy. However, since most commercial preparations of formaldehyde contain methanol, a protein precipitant, formaldehyde has been considered an unsatisfactory fixative for tissues to be examined by electron microscopy.In 1973, Carson et a/, described a parallel study comparing the electron microscopic results of fixation with paraformaldehyde vs. formaldehyde. They found that there was no difference in the preservation of ultrastructural morphology provided that the buffer systems were identical. In 1976, McDowell and Trump described a fixative combining commercial formaldehyde and glutaraldehyde (4CF-1G). Both of these fixatives are dual purpose fixatives and preclude the selection of tissue for electron microscopy prior to fixation. They can both be prepared in large quantities and used for routine surgical specimens.

Role of Electron Microscopy in Transplant Renal Pathology

1997 ◽  
Vol 21 (6) ◽  
pp. 481-498 ◽  
Author(s):  
Guillermo A. Herrera ◽  
Jorge Isaac ◽  
Elba A. Turbat-Herrera
Keyword(s):  
Electron Microscopy ◽  
Renal Pathology

Minimal change disease

2019 ◽  
pp. 175-224
Author(s):  
Claudio Ponticelli ◽  
Richard J Glassock ◽  
Rosanna Coppo
Keyword(s):  
Electron Microscopy ◽  
Nephrotic Syndrome ◽  
Light Microscopy ◽  
Renal Biopsy ◽  
Minimal Change Disease ◽  
The Elderly ◽  
Minimal Change ◽  
Common Cause ◽  
Foot Process

This chapter discusses minimal change disease (MCD), which is chiefly characterized clinically by episodes of nephrotic syndrome (NS) and presents with massive proteinuria, hypo-albuminaemia, hyperlipidaemia, and generalized oedema, Morphologically, it is characterized by no or only minimal glomerular abnormalities in a renal biopsy examined by light microscopy and immunofluorescence, while there is diffuse effacement of the podocyte foot process by electron microscopy. MCD is the most common cause of NS in children but it may also develop at any age, including in the elderly. This chapter covers the pathology, presentation, and treatment of MCD, including practical tips for the practitioner.

scholarly journals The role of electron microscopy for the diagnosis of glomerulopathies

2004 ◽  
Vol 122 (3) ◽  
pp. 104-109 ◽  
Author(s):  
Angelo Sementilli ◽  
Luiz Antonio Moura ◽  
Marcello Fabiano Franco
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Laboratory Data ◽  
Final Diagnosis ◽  
Glomerular Diseases ◽  
Financial Pressure ◽  
Renal Biopsies ◽  
Morphological Diagnosis ◽  
Selection Of

CONTEXT: Electron microscopy has been used for the morphological diagnosis of glomerular diseases for more than three decades and its value has been widely emphasized. However, recent reports have analyzed the routine use of electron microscopy critically. Its use in other areas of diagnosis such as tumor diseases has declined considerably; in addition, in view of the unavoidable financial pressure for the reduction of costs due to investigations and diagnostic routines, the selection of cases for electron microscopy has been quite rigorous. OBJECTIVE: To identify the glomerular diseases that depend on electron microscopy for a final diagnosis, by means of reviewing renal biopsies performed over a 12-year period. DESIGN: Prospective SETTING: Hospital Ana Costa, Hospital Guilherme Álvaro and Serviço de Anatomia Patológica de Santos, Santos, São Paulo, Brazil. PARTICIPANTS: 200 consecutive renal biopsies obtained from private hospitals and the teaching hospital from 1979 to 1991 were studied. MAIN MEASUREMENTS: All cases were analyzed via light microscopy, immunofluorescence and electron microscopy. The diagnosis was first made via light microscopy plus immunofluorescence and then via electron microscopy. RESULTS: Electron microscopy was diagnostic or essential for diagnosis in 10.0% of the cases, corresponding to 3.4% of primary glomerulopathies and 100% of hereditary glomerulopathies. Electron microscopy was contributory (useful) to the diagnosis in 5.5% of the cases, confirming the preliminary diagnosis formulated on the basis of clinical and laboratory data and light microscopy plus immunofluorescence findings. We obtained a 7.5% rate of discordant immunofluorescence, which was considered as such when negative immunofluorescence findings were not confirmed by electron microscopy. The final diagnosis with the use of light microscopy plus immunofluorescence alone was 77.0%. CONCLUSIONS: It was possible to diagnose with certainty a great percentage of glomerulopathies (82.5-90% of the cases) based on the light microscopy and immunofluorescence findings alone. Electron microscopy was essential for the diagnosis of hereditary nephropathies.

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