scholarly journals In vitro Cultivation of Newly Reported Wild Edible Mushroom Volvariella bomybycina from Nepal

2017 ◽  
Vol 5 (1) ◽  
pp. 27-31
Author(s):  
Pradip Kumar Chaudhary ◽  
Mitesh Shrestha ◽  
Bal Hari Poudel ◽  
Mahesh Kumar Adhikari

Wild edible mushrooms are becoming endangered all over the world. Very few wild edible mushrooms are found in natural habitat. Volvariella bombycina is an edible and medicinal mushroom. The mushroom was collected in natural habitat growing on Populus tree. Mycelium of the mushroom was developed in PDA slant tubes by tissue culture method, incubated at 25°C for 1-2 weeks. Spawn was developed in wheat grains after incubation at 25°C for 2-3 weeks. Substrates were formulated for the development of fruiting bodies by combination of paddy straw, saw dust and rice husk. Fruiting bodies of V. bombycina was cultivated in these substrates after incubation at 28 ± 2°C for 2-4 weeks. The work describes the optimized process for in vitro culture of wild edible mushroom Volvariella bomybycina.Nepal Journal of Biotechnology. Dec. 2017 Vol. 5, No. 1: 27-31

2021 ◽  
Vol 27 (4) ◽  
pp. 4157-4163
Author(s):  
Tsonka Dimitrova ◽  
◽  
Petko Marinov ◽  
Galina Yaneva ◽  
Dobri Ivanov ◽  
...  

Purpose: The interest in evaluation of real knowledge of wild edible mushrooms at individual and population level increases mainly in developing countries. The purpose of our inquiry study was to assess the level of knowledge and awareness of the wild mushrooms in a random sample from the population of Varna region in Bulgaria. Material/Methods: We performed an anonymous questionnaire investigation of 200 adult individuals, 100 males and 100 females, from Varna region, including 17 potential opinions from their personal knowledge of wild edible mushroom usage. Statistical data processing was performed using descriptive methods, variation and correlation analyses. Results: Most respondents acquired their knowledge of wild edible mushrooms from their relatives. From all 200 persons investigated 78 respondents were not capable of recognizing at least one wild edible mushroom at all. More respondents trusted other people, who have very good experience with preparation of wild mushrooms, thus relying on differentiation of a mushroom poison. Their awareness was associated with their capacity to properly recognize wild mushrooms as well as their long-term practice to recognize toxic mushrooms. Just a minority of respondents shared that wild mushrooms in their vicinity could be a problem and had a slight idea of mycotherapy. Only two females had heard about training for wild mushroom recognition. There were statistically significant correlation dependences between respondent’s awareness of wild edible mushrooms, on one hand, and individual sex status, educational level and main residence, on the other hand. Conclusion: Our study identified two most recognized wild edible mushroom species in Varna region, edible boletus (Boletus edulis Bull) and parasol mushroom (Macrolepiota procera S. F. Gray). Most respondents acquired their knowledge of wild edible mushrooms from relatives. A problem-oriented training of the population on this topic should be performed to improve awareness of common mushroom features and avoid mushroom poisoning.


Jurnal Wasian ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 121-135
Author(s):  
Ivan Permana ◽  

Belitong Island, The Province of Bangka Belitung is one of the exotic places with the biodiversity which has not been optimally inventoried. One of the potential germplasm that has not been recorded properly on Belitong Island is the mushrooms diversity and their potential uses. Mushroom are known as non-timber forestry product that is often sought after by local ethnic of Belitong Island and used as food. Apart from being consumed, these wild mushrooms are also traded, so they are one of the important seasonal commodities for the local community. This research aimed to provide the basic taxonomical information of wild edible mushroom in Belitong island as well as the biological characters. Observations were conducted 2 times in 2018 – 2019 using opportunistic sampling method.The utilization data of wild edible mushroom were collected to complete the macrofungi description in this study. A number of 5 edible wild mushrooms with th eeconomic value reported in this study were: Amanita sect. caesarea (kulat pelandok), Heimioporus sp. (kulat pelawan), Hygrocybe cf. conica (kulat tiong), Phylloporus sp. (kulat sukatan), and Volvariella sp. (kulat sawit). Three species of fungi are ectomycorrhizal forming fungi, namely kulat pelandok with Schima wallichii, kulat pelawan with Tristaniopsis merguensis, and kulat sukatan which host is still unclear. Meanwhile the rest are saprophytic macrofungi. All wild edible mushrooms are members of the phylum Basidiomycota which are divided into 2 orders and 4 families. Heimioporus sp. is known to be the highest selling price mushroom compared to the others, which indicated the potential prospect to proceed to the cultivation stage. In addition, conservation management of plants that are hosts of ectomycorrhizal fungi need to be an important concern on Belitong Island.


2018 ◽  
Vol 35 (1) ◽  
pp. 121-125
Author(s):  
K. K. Dahal ◽  
S. D. Joshi

Asparagus racemosus Willd. locally known as Kurilo or Shatavari is found throughout the tropical and subtropical regions of Nepal. It is a high value herb because of its medicinal and nutritional values which is leading this species decrease in its natural habitat. Hence, to conserve this species, an in vitro tissue culture method of its multiplication has been applied as an effective method of ex situ conservation. In the experiment, although most of the treatments induced highly insignificant number of adventitious shoots, low concentration of IBA (0.1-0.5 mg/l) in combination with relatively higher concentrations of Kinetin (1.0-2.0 mg/l) in MS medium were found to be significantly inducing up to 8.33±1.308 shoots/node. Among the treatments where hormones were used singly, IAA 0.5 and Kinetin 1.0 induced 4.83±1.08 and 4.66±1.43 shots/node respectively. Hence, a protocol for the effective multiplication of this species has been developed which can be used accordingly.


2021 ◽  
Vol 10 (3) ◽  
pp. 180-184
Author(s):  
Odangowei Inetiminebi Ogidi ◽  
◽  
Lilian Maureen Oluchi Oguoma ◽  
Patrick Chukwudi Adigwe ◽  
Blessing Bumein Anthony ◽  
...  

Microorganism resistance to synthetic antibiotics is an increasing public health challenge, therefore, new antimicrobial agents from different plant and biological sources are sought-after. This research was aimed at evaluating the phytochemical properties and antimicrobial potency of wild edible mushrooms. Qualitative and quantitative phytochemical analysis was done using the standard method of Association of Official Analytical Chemist and agar well diffusion method was used for antimicrobial analysis. Phytochemical results of wild edible mushroom Pleurotus ostreatus obtained from Yenagoa, shows varying quantities of tannins, terpenoids, alkaloids, flavonoids, saponins, glycosides and steroids. The methanol and aqueous extracts of the samples reveals different degree of inhibition on both bacteria and fungi (Shigella sp., Staphylococcus sp., Vibrio sp., Escherichia coli, Penicillium sp., Yeast and Moulds). Aqueous extracts were more effective on the test organisms when compared to methanol extracts. P. ostreatus showed good antimicrobial potency against all the microorganisms tested. The findings from this study show the efficacy of wild edible mushroom as a potent antimicrobial agent


2021 ◽  
Vol 27 (4) ◽  
pp. 4151-4156
Author(s):  
Tsonka Dimitrova ◽  
◽  
Petko Marinov ◽  
Galina Yaneva ◽  
Dobri Ivanov ◽  
...  

Purpose: Wild edible mushrooms are collected and consumed for centuries worldwide and in Bulgaria as well. This questionnaire investigation aimed at assessing some common mushroom usage practices in a random sample from the population in Varna region, Bulgaria. Material/Methods: We performed a study of 200 adult individuals, 100 males and 100 females, from Varna region using anonymous questionnaire with 12 basic items about wild edible mushroom usage. Statistical data processing was performed using descriptive methods and correlation analysis. Results: There was a prevalence of male and female individuals with secondary and higher education. Most respondents preferred wild edible mushroom gathering in contrast to purchasing. The positive attitude towards wild edible mushroom usage as food is more common in males than in females. Most respondents consumed wild edible mushroom several times during any year. There were statistically significant correlations between urban main residence, on one hand, and wild edible mushroom non-nutritional usage, cooking, drying, preservation and freezing, on the other hand; between secondary educational level, on one hand, and positive attitude towards nutritional usage of wild edible mushrooms, wild edible mushroom cooking, preservation, freezing and drying and applications as therapeutic means; as well as between individual female sex belonging and wild edible mushroom applications as therapeutic means, on the other hand. Conclusion: This questionnaire investigation revealed several interesting peculiarities of the attitudes towards wild edible mushrooms among the population of Varna region and the extent of usage of common mushrooms depending on sex, educational level and main residence of respondents.


2020 ◽  
Vol 26 (39) ◽  
pp. 4970-4981
Author(s):  
Yu-Tang Tung ◽  
Chun-Hsu Pan ◽  
Yi-Wen Chien ◽  
Hui-Yu Huang

Metabolic syndrome is an aggregation of conditions and associated with an increased risk of developing diabetes, obesity and cardiovascular diseases (CVD). Edible mushrooms are widely consumed in many countries and are valuable components of the diet because of their attractive taste, aroma, and nutritional value. Medicinal mushrooms are higher fungi with additional nutraceutical attributes having low-fat content and a transisomer of unsaturated fatty acids along with high fiber content, biologically active compounds such as polysaccharides or polysaccharide β-glucans, alkaloids, steroids, polyphenols and terpenoids. In vitro experiments, animal models, and even human studies have demonstrated not only fresh edible mushroom but also mushroom extract that has great therapeutic applications in human health as they possess many properties such as antiobesity, cardioprotective and anti-diabetic effect. They are considered as the unmatched source of healthy foods and drugs. The focus of this report was to provide a concise and complete review of the novel medicinal properties of fresh or dry mushroom and extracts, fruiting body or mycelium and its extracts, fiber, polysaccharides, beta-glucan, triterpenes, fucoidan, ergothioneine from edible mushrooms that may help to prevent or treat metabolic syndrome and associated diseases.


Blood ◽  
1967 ◽  
Vol 29 (6) ◽  
pp. 852-858
Author(s):  
YASUSADA MIURA ◽  
FUMIMARO TAKAKU ◽  
KIKU NAKAO

Abstract 1. An in vitro method to observe radiosensitivity of stem cells was developed in the present study. In vivo and in vitro effect of 60Co irradiation on the erythropoietin-induced stem cell differentiation into erythroblasts was observed, using a tissue culture method of polycythemic mouse spleen. Response to erythropoietin was demonstrated by an appearance of heme synthesis and erythroblasts in spleen fragments. 2. A significant correlation between the rate of appearance of erythroblasts and heme synthesis of the spleen fragments was observed. 3. After irradiation, marked impairment of both heme synthesis and production of erythroblasts was observed, yielding D37 values in the vicinity of 70 r in vivo and 120 r in vitro irradiation, respectively. 4. Marked recovery of erythropoietin-induced heme synthesis in the polycythemic mouse spleen was observed 9 days after 300 r irradiation, with an "overshooting" phenomenon on the 12th day.


2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Madhavi Joshi ◽  
Anand Sagar

Bioactive compounds and antioxidant activity of methanolic extract of Sparassis crispa collected from North Western Himalayan region of India were analyzed. Phenolic content 11.14±0.08 mg tannic acid equivalent per g of the extract and flavonoids 1.96±04 mg catechin equivalent per g of the extract were recorded to be the major antioxidant components in this wild edible mushroom. Significant antioxidant efficiency on inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH) was observed when compared to standard antioxidant like L-ascorbic acid. IC50 value of the extract was 2.11 mg/mL. The findings suggest S. crispa as an easily accessible source of natural antioxidants.


2021 ◽  
Author(s):  
Praxedis Dube ◽  
Godwil Madamombe ◽  
Linda Tapfumaneyi ◽  
Wonder Ngezimana ◽  
Kennedy Simango

Abstract This study evaluates the importance of wild edible mushrooms as food in three villages of the Binga, Zimbabwe. A purposive sampling method was employed to select three villages from two (Local Government Area) wards, 25 households per each village randomly selected from the total household list provided by the district administration department, with a total of 75 households. An open ended, structured semi structured interview guide was used to provide a preliminary list of wild edible mushrooms in the area and the identification methods used, using seven households from the three villages. Structured, semi-structured and guided forest walks with some of the informants and forest picking observation was carried out with local guide assistants who have the skills of local (Tonga) language and Shona language. An econometric model was used to identify the factors influencing wild edible mushroom consumption in the study area. Descriptive statistics was used to describe the socio-economic characteristics of the respondents. Results from purposive sampling showed that seven ethnospecies of wild edible mushrooms were listed (in Tonga and scientific names in parenthesis: Bakapyapya (Cantharellus spp), Bakayanda (Amanita loosii (zambiana)), Bwabbaya (Termitomyces spp), Indyuu (Termitomyces titanicus), Madongila (Amanita spp), Mbuse (Termitomyces clypeatus), Nowa (Lactarius kabansus) and Indyuu, Madongila, Nowa) and of these three were noted as extinct species (Indyuu, Madongila, Nowa). About 43% of the participants consumed wild edible mushrooms once every day, 19% twice every day and 14% every second day during the rainy season, underpinning the potential importance of wild edible mushrooms for food security. Around 52% of the participants collected wild edible mushrooms once every day, 28.6% of the every second day while around 16% collected once a week, during the rainy season. Ethnobotanical knowledge of wild edible mushrooms was mainly passed orally from elderly to young people (72.9% of the participants), while 15.7% passed by mothers to their children and 11% learned from female elders to young women and girls. Identification techniques such as texture feeling, colour underneath and on top, breaking and observing milk like liquid oozing out and points of collection were used. Women (21.4%), women accompanied by girl child (24.3%) are especially involved in wild edible mushrooms collection, while a minority 1.4% men, women accompanied by boy child (7.1%) did the collection. The mean monthly income per household is US$108.57, largest in Simandala and lowest in Dumbwe. The average age of the participants is 45.37 years. Of the 70 participants, 61.4 were female and 38.6 were male. Average consumption of wild edible mushrooms per meal per household is 1.436kg. About 95.7% of the participant served wild edible mushrooms as main relish, accompanying the staple. The mostly consumed were Indyuu (Zhouchuru) and Bakayanda (Nhedzi), consumed by 100% of the participants. Linear regression showed that the coefficients were positive and not significant at p<0.05 level, only significant on household size.


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