scholarly journals Detection of Pyuria versus Bacteriuria in Suspected Patients of Urinary Tract Infection

2015 ◽  
Vol 15 (1) ◽  
pp. 129-132
Author(s):  
Anjila Dongol ◽  
Durga Man Joshi ◽  
Avishekh Gautam
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Culture Technique ◽  
Urine Samples ◽  
Suspected Case ◽  
Urine Sediment ◽  
Mortality And Morbidity ◽  
Significant Bacteriuria ◽  
Culture Positive

Urinary tract infection (UTI) is one of the most important causes of mortality and morbidity in the world affecting all age groups across the life span. The aim of this study is to determine incidence of bacteriuria and its relation with pyuria in suspected urinary tract infection. This crosssectional-descriptive-analytical study was conducted in microbiology section of Kathmandu Hospital, Tripureshwor from May to August 2011. During this period, 412 midstream urine samples collected were investigated by microscopic examination of centrifuged urine sediment and conventional semi-quantitative culture technique. Out of 412 MSU samples, 120 (29.13%) were culture positive. The remaining 22 (18.33%) samples showed low count significant bacteriuria (i.e 104-105 CFU/ml) and 98(81.67%) showed significant bacteriuria (i.e. ≥ 105 CFU/ml). Among 88 urine samples with significant pyuria, only 74   (84.09%) showed culture positive. Out of 324 urine samples without significant pyuria, 46 (14.19%) urine samples showed culture positive result. Significant pyuria was found to have sensitivity of 61.67%, specificity of 95.21% and the test has 84.09% positive predictive value in detecting bacteriuria. Pyuria and bacteriuria may not always correlate in the suspected case of UTI.DOI: http://dx.doi.org/10.3126/njst.v15i1.12029Nepal Journal of Science and TechnologyVol. 15, No.1 (2014) 129-132

scholarly journals PSEUDOMONAS SPECIES CAUSING URINARY TRACT INFECTION AND ITS ANTIBIOGRAM AT A TERTIARY CARE HOSPITAL

2017 ◽  
Vol 10 (11) ◽  
pp. 50
Author(s):  
Shobha Kl ◽  
Ramachandra L ◽  
Amita Shobha Rao ◽  
Anand Km ◽  
Gowrish Rao S
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Urine Samples ◽  
Automated System ◽  
Care Hospital ◽  
Pseudomonas Species ◽  
Significant Bacteriuria

  Objectives: Pseudomonas species cause urinary tract infection (UTI). This study was conducted to isolate Pseudomonas species causing significant bacteriuria and to analyze its antibiogram at a tertiary care hospital.Methods: About 107 urine samples received in the laboratory from August 2015 to July 2016 having significant bacteriuria with Pseudomonas species were tested for antimicrobial activity. Samples were from both inpatients and patients attending as outpatients to a tertiary care hospital with different age groups and gender. Midstream urine samples were inoculated using a standard calibrated loop into blood agar and MacConkey agar following semi quantitative technique using Kass concept. MALD (Biomerio, ElToile, France) was used for speciation, and Vitex automated system (Biomerio, ElToile, France) was used for antibacterial sensitivity testing.Results: About 107 urine samples contained Pseudomonas species by MALDI obtained from 69 male (64.48%) and 38 (35.51%) female patients. Inpatients were 90 (84.11%) and 17 (15.88%) outpatient department (OPD) patients. Isolation of Pseudomonas aeruginosa was from 90 samples (84.11%) and Pseudomonas putida in 17 (15.88%). Significant bacteriuria was more in the age group of ˃60 years in both genders. Susceptibility to gentamicin was 52 (48.59%), ceftazidime 56 (52.33%), and imipenem was 58 (54.20%). Organism was multidrug resistant in 49 (45.79%) samples.Conclusion: P. aeruginosa remains the most common uropathogen among Pseudomonas species. Drug of choice for inpatients suffering from UTI by Pseudomonas species can be gentamicin and for patients attending OPD can be ceftazidime. Imipenem can be the reserve drug. Periodic antibiotic review is required for proper treatment of UTI.

scholarly journals Asymptomatic Bacteriuria among Pregnant Women Attending a Tertiary Care Hospital in Western Nepal: A Cross-sectional Prospective Study

2021 ◽  
Vol 9 (01) ◽  
pp. 38-42
Author(s):  
Shristi Raut ◽  
Sulochana Khatiwada ◽  
Narayan GC
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Pregnant Women ◽  
Asymptomatic Bacteriuria ◽  
Urine Samples ◽  
Diffusion Method ◽  
Care Hospital ◽  
Significant Bacteriuria

INTRODUCTION Urinary tract of female undergoes tremendous changes during pregnancy which increases their risk of acquiring infection. Asymptomatic bacteriuria is a common entity among pregnant women which refers to significant bacteriuria (>105 bacteria per ml of urine) without any typical symptoms of urinary tract infection. Asymptomatic bacteriuria can lead to various maternal and fetal complications if not detected and treated on time. MATERIAL AND METHODS Total 280 urine samples were collected in sterile universal containers from pregnant women not showing typical symptoms of urinary tract infection at the time of sample collection. Urine samples were inoculated in both MacConkey agar and Blood agar by semi quantitative culture method. Culture plates were reported after 24 hours of incubation at 370C. Bacteria isolated were subjected to antibacterial susceptibility testing by modified Kirby-Bauer disc diffusion method. RESULTS Out of 280 urine cultures, 213 samples were sterile. Significant bacteriuria was seen in 25 cases (8.9%) followed by insignificant bacteriuria (20, 7.14%) and contamination (17, 6.10%). Highest number of cultures positive were in age group 21-30 years (19, 9.1%,). Out of 25 cases of significant bacteriuria, 60% were primigravida and 40% were multigravida. The most common organism isolated was Escherichia coli (10, 60%) followed by Klebsiella pneumoniae (5, 40%). CONCLUSION Screening of all pregnant women for asymptomatic bacteriuria is essential during their antenatal checkup. Escherichia coli is the commonest organism that cause asymptomatic bacteriuria. Appropriate antibiotic therapy is absolutely necessary for positive cases on the basis of antimicrobial susceptibility result to prevent unwanted obstetric complications.

scholarly journals Urinary tract bacterial infection of local Iraqi buffaloes (Bubalus Bubalis) in Mosul city

2017 ◽  
Vol 40 (2) ◽  
pp. 124-130 ◽  
Author(s):  
O. M. Al-Iraqi
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Bubalus Bubalis ◽  
Urine Samples ◽  
Buffalo Calves ◽  
Urine Ph ◽  
Urine Sediment ◽  
Chemical Examination ◽  
Normal Urine

     This study is conducted on the samples of urine obtained from buffaloes. Eighty six urine samples were obtained from local buffaloes of different ages, of both sex in Mosul abattoir. The results showed that urine of infected animals was cloudy, dark yellow to reddish color with putrid odor of low specific gravity. Microscopic examination of urine sediment demonstrated increased number of pus cell, erythrocytes, epithelial cell, presence of casts and crystals/ high power filed. Chemical examination revealed elevated urine pH and protein content. Serum examination displayed increase levels of urea and creatinine compered to normal urine samples. Culture of urine samples confirmed 24 positive samples (27.90%). Corynebacterium renale being the most isolated organisms 12(50%), Trueperella pyogens 5(20.83%), Staphylococcus aureus 4(16.66%) and 1(4.16%) for each of Acinetobacter lwoffii, Corynebacterium pilosum and Corynebacterium cystitidis. Male buffalo calves at age 1-3 years revealed high percentage (18.60%) of urinary tract infection, while low percentage of urinary tract infection existed by female buffaloes age more than 3 years (6.98%).        

scholarly journals Drug Susceptibility Pattern and Associated Factors of Bacteria Isolated from Urinary Tract Infection among Pregnant Women Attending Antenatal Care in Teaching Hospital, Southeast Ethiopia

2021 ◽  
Author(s):  
Eshetu Nigussie ◽  
Meseret Mitiku ◽  
Alelign Tasew ◽  
Biniyam sahiledengel ◽  
Damtew Solomon ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Pregnant Women ◽  
Previous History ◽  
Urine Samples ◽  
P Value ◽  
Susceptibility Pattern ◽  
Southeast Ethiopia ◽  
Significant Bacteriuria

Abstract Background: Urinary tract infection is caused by bacteria from the digestive tract which climbs the opening of the urethra and begins to multiply to cause infection. This study aimed to assess the bacterial profile and antibiotic susceptibility pattern and associated risk factors among pregnant women in Madda Walabu University Goba Referral Hospital, Southeast Ethiopia. Methods: An institution-based cross-sectional study was conducted from June-August, 2019. A total of 234 pregnant women were enrolled; data were collected using a structured questionnaire by a trained interviewer. Urine samples were collected from all pregnant women and culture on cysteine lysine electrolytes deficiency medium. Data were analyzed using SPSS 20.0. Descriptive statistics were used to explain the study participants with relevant variables. Logistic regression was used for data comparison. P-value <0.05 was accepted as statistically significant. Results: The overall prevalence of UTI was 23.9% among both asymptomatic and symptomatic groups. Of this screened midstream urine samples showed that 16% and 32.2%, had significant bacteriuria in the asymptomatic and symptomatic groups respectively. The prevalence of UTI was significantly associated with previous history of catheterization and urinary tract infection. E. coli was the most frequently isolated organism (42.9%) followed by coagulase-negative Staphylococcus (26.8%), and S. aureus (12.5%). Gram-negative and Gram-positive bacteria accounted for (59%) and (41%) respectively.Conclusion: Significant bacteriuria has been observed from both symptomatic and asymptomatic pregnant women. The majority of the isolates were resistant to commonly prescribed antibiotics. This calls for an early screening of all pregnant women for UTI and those found to be infected need treatment.

CORRELATION OF ABDOMINAL ULTRASONOGRAPHY FINDINGS WITH THE AGE OF CHILDREN WITH CULTURE POSITIVE URINARY TRACT INFECTION

2021 ◽  
pp. 46-48
Author(s):  
Murchana Khound ◽  
Sekharjyoti Sharma
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Culture ◽  
Laboratory Data ◽  
Abdominal Ultrasonography ◽  
Female Ratio ◽  
Mortality And Morbidity ◽  
Male Female Ratio ◽  
Culture Positive

Background: Urinary tract infection(UTI) is an important cause of mortality and morbidity in children, especially complicated UTI. In this study we have tried to see the correlation of Ultrasound abdomen ndings with age of the culture positive UTI patients. Objectives: to look at the age and sex distribution of patients with culture positive UTI and to look for the correlation of ultrasound abdomen ndings with the age of culture positive UTI patients Methods: It was a hospital based observational study conducted in one of the busiest hospitals of Jorhat over a period of 12 months. Urine culture reports of all patients were traced from the hospital laboratory data. Ultrasound abdomen was done in patients with positive urine culture report . Results: Total 206 urine samples were tested from suspected cases out of which 43(21%) were culture positive urinary tract infection. UTI was more in girls as compared to boys. The male : female ratio was found to be 3:1 in children less than 2 years and in children more than 2 years female outnumbered males(1:2 , 2:3 and 1:6 in children between the age group of 2 to 5 years, 6-9years and 10 to 14 years respectively). Cystitis(p=0.22) and hydronephrosis(0.27) had no signicant correlation with the age of the UTI patients whereas Pyelonephritis(p=0.03) had signicant correlation with age of UTI patients. All the patients who had pyelonephritis were less than 5 years of age. Conclusion: UTI is an important cause of mortality and morbidity in children and younger children are more prone to complications. So a collective effort should be made to diagnose such patients earlier and treat them adequately with judicious use of antibiotics.

scholarly journals Non-Fermenting Gram Negative Bacteria as Uropathogens in Causing Urinary Tract Infection and its Antimicrobial Susceptibility Pattern at A Tertiary Care Centre of South India

2020 ◽  
Vol 14 (3) ◽  
pp. 2033-2038
Author(s):  
Anupam Berwal ◽  
K.L. Shobha ◽  
Rohit Gupta ◽  
Kriti Gupta ◽  
Asem Ali Ashraf ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Tertiary Care ◽  
Urine Samples ◽  
Age Group ◽  
Susceptibility Pattern ◽  
Comorbid Conditions ◽  
Gram Negative ◽  
Significant Bacteriuria

Non fermenting gram-negative bacilli (NFGNB) are recently striving as uropathogens. The present study was conducted to isolate the common species of bacteria in NFGNB causing urinary tract infection (UTI) and its correlation with comorbid conditions and to study the antibacterial susceptibility pattern. This retrospective study was done at the diagnostic Microbiology laboratory of a tertiary care hospital. Urine samples were collected for the period of six months. These samples were plated on blood agar and MacConkey agar and incubated at 37°C for 18–24 hr under aerobic conditions. Identification of NFGNB was done by Gram staining and MALDI-TOF (Matrix- Assisted Laser Desorption/ Ionization- Time of Flight, Biomerieux- Diagnostics). Antibiotic sensitivity testing was done by Vitek® 2 system (Biomerieux- Diagnostics) using N 281 card. Data was analyzed using SPSS IBM version 16. Out of the total 16,413 non repetitive urine samples that were received in the laboratory, 318 had significant bacteriuria. NFGNB were identified in 108 (33.9%) of all the urine samples with significant bacteriuria. Prevalence of non-fermenters in our study was 0.6%. NFGNB were more frequently isolated in the females and also in the age group of more than 50 years. Eighty five (78.70%) had comorbid conditions. P. aeruginosa and A. baumannii were the most common organism isolated among NFGNB. Pseudomonas aeruginosa isolates showed high susceptibility to imipenem (80.2%) and amikacin (66.6%). NFGNB although seen frequently in females and in age group of 50 years and above, clinical correlation with comorbid condition is essential to label it as uropathogens. Amikacin or imipenem may be the empirical drug of choice.

scholarly journals Culture-positive urinary tract infection following micturating cystourethrogram in children

2021 ◽  
Author(s):  
Ngweso Simeon ◽  
Nyandoro Munyaradzi ◽  
Nzenza Tatenda ◽  
Cheow Ting Yi ◽  
Bettenay Fiona ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Micturating Cystourethrogram ◽  
Culture Positive

scholarly journals Purification, Extraction and Visualization of Lipopolysaccharide of Escherichia coli From Urine Samples of Patients With Urinary Tract Infection

2015 ◽  
Vol 2 (4) ◽  
pp. 31990-31990 ◽  
Author(s):  
Fariha Akhter Chowdhury ◽  
Mohammad Nurul Islam ◽  
Anamika Saha ◽  
Sabrina Mahboob ◽  
Abu Syed Md. Mosaddek ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Samples

scholarly journals Clinical, Epidemiological and Bacteriological Profile of Culture Positive Urinary Tract Infections in Febrile Children – A Cross Sectional Study

2021 ◽  
Vol 8 (10) ◽  
pp. 522-526
Author(s):  
Bhavani Shankar Rokkam ◽  
Chowdary Babu Menni ◽  
Ramu Pedada ◽  
Deepak Kumar Alikana
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urinary Tract Infections ◽  
Urine Culture ◽  
Signs And Symptoms ◽  
Cross Sectional ◽  
Bacteriological Profile ◽  
Tract Infections ◽  
Culture Positive

BACKGROUND Urinary tract infections (UTI) constitute a common cause of morbidity in infants and children. When associated with abnormalities of urinary tract, they may lead to long-term complications including renal scarring, loss of function and hypertension. Most urinary tract infections remain undiagnosed if investigations are not routinely performed to detect them. Prompt detection and treatment of urinary tract infections and any complicating factors are important. The objective of the study is to know the clinical, epidemiological and bacteriological profile (i.e. clinical signs and symptoms, age, sex, family history, associated urinary tract abnormalities, & causative organisms) of urinary tract infections in febrile children with culture positive urinary tract infection. METHODS This descriptive, cross sectional observational study was conducted at outpatient clinics of our “child health clinics” between May 2016 and April 2017 (one year). All children aged 0 to 12 years with culture positive urinary tract infections were included in this study to evaluate the clinical, epidemiological and bacteriological profile. RESULTS A total of 69 children with culture positive urinary tract infections were included in this study. Out of 69 children included in this study, 36 (52.2 %) were females and 33 (47.8 %) were males. Overall female preponderance was seen and the M: F ratio was 0.9:1. But during first year of life in our study group we had more boys (10, 14.49 %) affected with urinary tract infection than girls. 49.3 % of urinary tract infections in the present study belonged to lower socio-economic status. Most common organism causing urinary tract infection in our group was E. coli (56.5 %). Fever (100 %), anorexia or refusal of feeds (52.2 %), dysuria (46.4 %), vomiting (46.4 %) and abdominal pain (39.1 %) were the predominant clinical manifestations observed in our study. CONCLUSIONS Urinary tract infection is a common medical problem in children and it should be considered as a potential cause of fever in children. As febrile children with urinary tract infection usually present with non-specific signs and symptoms, urine culture should be considered as a part of diagnostic evaluation. KEYWORDS Urinary Tract Infections (UTI), Febrile Children, Bacteriological Profile, Urine Culture

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