scholarly journals Ecophysiology of Mimosa pudica L. at Biratnagar, Eastern Nepal

Our Nature ◽  
1970 ◽  
Vol 7 (1) ◽  
pp. 177-181 ◽  
Author(s):  
B. Niroula ◽  
D. Parajuli ◽  
S. Jha
Keyword(s):  
Seed Coat ◽  
Crude Protein ◽  
Dry Weight ◽  
Germination Percentage ◽  
Mimosa Pudica ◽  
Crude Fibre ◽  
Hard Seed ◽  
Total Plant ◽  
Mature Seeds ◽  
Hard Seed Coat

All the freshly collected mature seeds of M. pudica were greenish in colour, whereas 7 year old stored seeds were a mixture of greenish and brownish seeds. The greenish seeds had hard seed coat and acid treated as well as sand rubbing for 6-10 min greatly enhanced their germination percentage. The brownish seeds were simply the deteriorated form of greenish seeds. Leaves and pods had higher contribution to total plant dry weight in ungrazed field and in pot-cultured plants clipped a month before flowering. The young shoots of the plant had 18.9% crude protein, 0.46% phosphorus and 25.4% crude fibre on dry weight basis.Key words: Hard seed coat, Mimosa pudica, scarification, dry matter productionDOI: 10.3126/on.v7i1.2568Our Nature (2009) 7:177-181  

scholarly journals Propagation of threatened climber species Entada rheedii Spreng. – a medicinal plant with extremely thick and hard seed coat

Dendrobiology ◽  
2021 ◽  
Vol 85 ◽  
pp. 92-104
Author(s):  
Md. Aktar Hossain ◽  
Joyeta Dey ◽  
Mohammed Abdur Rahman
Keyword(s):  
Medicinal Plant ◽  
Growth Performance ◽  
Seed Coat ◽  
Dry Mass ◽  
Germination Percentage ◽  
Stem Cutting ◽  
Hard Seed ◽  
Collar Diameter ◽  
Survival And Growth ◽  
Hard Seed Coat

The study explored propagation techniques of Entada rheedii Spreng., a threatened medicinal climber species with extremely hard seed coat. Propagation trials included both pre-sowing treatments of seeds for germination and clonal propagation by stem cutting. Pre-sowing treatments included (a) soaking of both cut (notched) and uncut (intact) seeds in water for 0 h, 24 h, 48 h, and 72 h and (b) immersion of intact seeds in 5% acetone solution for 5 min, 10 min, and 20 min before sowing in germination media in polybags. On the other hand, stem cutting involved treating the summer or autumn cuttings with 0%, 0.4%, and 0.8% IBA solution before rooting in non-mist propagator. Notched seeds soaked in water for 48 h showed the fastest seed germination with the highest germination percentages (73.3) and better seedling growth in terms of plant height, collar diameter, leaf number and total dry mass followed by notched seeds soaked in water for 72 h. The slowest germination and the lowest germination percentage (3.3), as well as the poorest growth performance was for intact seeds without any treatment. The highest rooting percent­age with maximum number of roots (36.6) was obtained from the summer cuttings treated with 0.4% IBA solution followed by autumn cuttings with 0.8% IBA and the lowest (43.3% and 8.3 roots) was for summer cuttings in control. The factors also dictated the survival and growth performance of rooted cuttings in the nursery conditions. The outcomes of these trials i.e., notched seeds soaking in water for 48 h will help to enhance the propagation of this valuable medicinal plant species.

Dormancy breakage in Cercis chinensis seeds

2020 ◽  
Vol 100 (6) ◽  
pp. 666-673
Author(s):  
Yunpeng Gao ◽  
Mingwei Zhu ◽  
Qiuyue Ma ◽  
Shuxian Li
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Seed Coat ◽  
Germination Percentage ◽  
Physical Dormancy ◽  
Optimal Method ◽  
Hard Seed ◽  
Positive Effects ◽  
Physiological Dormancy ◽  
Hard Seed Coat

The seeds of Cercis chinensis Bunge are important for reproduction and propagation, but strong dormancy controls their germination. To elucidate the causes of seed dormancy in C. chinensis, we investigated the permeability of the hard seed coat and the contribution of the endosperm to physical dormancy, and we examined the effect of extracts from the seed coat and endosperm. In addition, the effectiveness of scarification methods to break seed dormancy was compared. Cercis chinensis seeds exhibited physical and physiological dormancy. The hard seed coat played an important role in limiting water uptake, and the endosperm acted as a physical barrier that restricted embryo development in imbibed seeds. Germination percentage of Chinese cabbage [Brassica rapa subsp. chinensis (L.) Hanelt] seeds was reduced from 98% (control) to 28.3% and 56.7% with a seed-coat extract and an endosperm extract, respectively. This demonstrated that both the seed coat and endosperm contained endogenous inhibitors, but the seed-coat extract resulted in stronger inhibition. Mechanical scarification, thermal scarification, and chemical scarification had positive effects on C. chinensis seed germination. Soaking non-scarified seeds in gibberellic acid (GA3) solution did not promote germination; however, treatment with exogenous GA3 following scarification significantly improved germination. The optimal method for promoting C. chinensis seed germination was soaking scarified seeds in 500 mg·L−1 GA3 for 24 h followed by cold stratification at 5 °C for 2 mo.

scholarly journals Perkecambahan Benih Aren (Arenga Pinnata) Setelah Diskarifikasi Dengan Giberelin Pada Berbagai Konsentrasi

2014 ◽  
Vol 2 (2) ◽  
pp. 71
Author(s):  
Oktoviani Purba ◽  
Indriyanto . ◽  
Afif Bintoro
Keyword(s):  
Seed Coat ◽  
Chemical Treatment ◽  
Germination Percentage ◽  
Percentage Germination ◽  
Physical Treatment ◽  
Random Design ◽  
Hard Seed ◽  
Hard Seed Coat

Dormancy of sugar palm seed (Arenga pinnata) was caused of hard seed coat structure, making its difficult to absorb water during of germinating. Dormancy in seed can be resolved by giving of treatmeant physically, mechanically, or chemically. This research aimed to determine the effect of physical treatment and chemical treatment on the germination of sugar palm seeds and determine which affect gibberellin concentration most favorable to the germination of sugar palm seeds. In this research, dormancy in seed be resolved in chemically that way with soaking of water with temperature early 75 0 C let to be chilled during 15 minute, and then soaking in condensation of giberelin with concentration of giberellins is 0 ppm, 50 ppm, 100 ppm, 150 ppm, and 200 ppm for 24 hours.  The method used in this research is Complete Random Design (CRD) which consisting of five treatments and four replications. The results showed that the treatment accorded significant effect on germination percentage, germination, and the average days to germinate. Addition of soaking in a solution of 150 ppm giberellin for 24 hours gives the best effect with an average germination percentage by 65%, compared with addition of soaking in giberellin solution of 0 ppm, 50 ppm, 100 ppm, 200 ppm giberellins for 24 hours with an average germination percentage by 15%, 34,5%, 53,125%, and 26,875%.

scholarly journals Asymbiotic germination of Vanilla planifolia in relation to the timing of seed collection and seed pretreatments

2021 ◽  
Vol 62 (1) ◽  
Author(s):  
Chih-Hsin Yeh ◽  
Kai-Yi Chen ◽  
Yung-I. Lee
Keyword(s):  
Seed Germination ◽  
Seed Development ◽  
Seed Coat ◽  
Germination Percentage ◽  
Vanilla Planifolia ◽  
Outermost Layer ◽  
Seed Collection ◽  
Seed Pretreatment ◽  
Immature Seeds ◽  
Mature Seeds

Abstract Background Vanilla planifolia is an important tropical orchid for production of natural vanilla flavor. Traditionally, V. planifolia is propagated by stem cuttings, which produces identical genotype that are sensitive to virulent pathogens. However, propagation with seed germination of V. planifolia is intricate and unstable because the seed coat is extremely hard with strong hydrophobic nature. A better understanding of seed development, especially the formation of impermeable seed coat would provide insights into seed propagation and conservation of genetic resources of Vanilla. Results We found that soaking mature seeds in 4% sodium hypochlorite solution from 75 to 90 min significantly increased germination. For the culture of immature seeds, the seed collection at 45 days after pollination (DAP) had the highest germination percentage. We then investigated the anatomical features during seed development that associated with the effect of seed pretreatment on raising seed germination percentage. The 45-DAP immature seeds have developed globular embryos and the thickened non-lignified cell wall at the outermost layer of the outer seed coat. Seeds at 60 DAP and subsequent stages germinated poorly. As the seed approached maturity, the cell wall of the outermost layer of the outer seed coat became lignified and finally compressed into a thick envelope at maturity. On toluidine blue O staining, the wall of outer seed coat stained greenish blue, indicating the presence of phenolic compounds. As well, on Nile red staining, a cuticular substance was detected in the surface wall of the embryo proper and the innermost wall of the inner seed coat. Conclusion We report a reliable protocol for seed pretreatment of mature seeds and for immature seeds culture based on a defined time schedule of V. plantifolia seed development. The window for successful germination of culturing immature seed was short. The quick accumulation of lignin, phenolics and/or phytomelanins in the seed coat may seriously inhibit seed germination after 45 DAP. As seeds matured, the thickened and lignified seed coat formed an impermeable envelope surrounding the embryo, which may play an important role in inducing dormancy. Further studies covering different maturity of green capsules are required to understand the optimal seed maturity and germination of seeds.

scholarly journals Asymbiotic Germination of Mature Seeds and the Seed Development of Vanilla Planifolia

2021 ◽  
Author(s):  
Chih-Hsin Yeh ◽  
Kai-Yi Chen ◽  
Yung-I Lee
Keyword(s):  
Cell Wall ◽  
Seed Germination ◽  
Seed Development ◽  
Seed Coat ◽  
Germination Percentage ◽  
Vanilla Planifolia ◽  
Outermost Layer ◽  
Seed Pretreatment ◽  
Immature Seeds ◽  
Mature Seeds

Abstract Background: Vanilla planifolia is an important tropical orchid for production of natural vanilla flavor. Traditionally, V. planifolia is propagated by stem cuttings, which produces identical genotype that are sensitive to virulent pathogens. However, sexual propagation with seed germination of V. planifolia is intricate and unstable because of the extremely hard seed coat. A better understanding of seed development, especially the formation of impermeable seed coat would provide insights into seed propagation and conservation of genetic resources of Vanilla.Results: We found that soaking mature seeds in 4 % sodium hypochlorite solution from 75 to 90 min significantly increased germination and that immature seeds collected at 45 days after pollination (DAP) had the highest germination percentage. We then investigated the anatomical features during seed development that associated with the effect of seed pretreatment on raising seed germination percentage. The 45-DAP immature seeds have developed globular embryos and the thickened non-lignified cell wall at the outermost layer of the outer seed coat. After 60 DAP, the cell wall of the outermost layer of the outer seed coat became lignified and finally compressed into a thick envelope. These features matches the significant decreases of immature seed germination percentage after 60 DAP. Conclusion: We report a reliable protocol for seed pretreatment of mature seeds and for immature seeds culture based on a defined time schedule of V. plantifolia seed development. The thickened and lignified seed coat formed an impermeable envelope surrounding the embryo, and might play an important role in seed dormancy of V. plantifolia.

The pattern of seed development and maturation in beach pea (Lathyrus maritimus)

2003 ◽  
Vol 81 (6) ◽  
pp. 531-540 ◽  
Author(s):  
Gurusamy Chinnasamy ◽  
Arya Kumar Bal
Keyword(s):  
Seed Development ◽  
Seed Coat ◽  
Dry Weight ◽  
Growth Stages ◽  
Protein Accumulation ◽  
Grass Pea ◽  
Hard Seed ◽  
Pea Seeds ◽  
Lathyrus Maritimus ◽  
Beach Pea

The developmental patterns of seed, seed coat, and hardseededness were studied in naturally growing crop plants of beach pea (Lathyrus maritimus (L.) Bigel.) at six reproductive growth stages (S1–S6). Grass pea (Lathyrus sativus L.) seeds were used for comparison in some experiments. The accumulation of fresh and dry weight in pod shell and seed of beach pea and pod shell of grass pea followed an almost sigmoidal pattern. However, grass pea seed showed a linear pattern of weight accumulation. During maturation, moisture content of pod shells and seeds decreased because of dehydration. Beach pea seeds were able to germinate precociously at S4. Seeds collected between S1 and S3 failed to germinate because of immaturity, whereas the development of hard seed coats prevented germination in seeds gathered at S5 and S6. An imbibition test revealed that hardseededness completely prevented water absorption of S5 and S6 seeds even after 24 days of soaking. In grass pea, precocious seed germination was observed at S3. However, speed of germination, germination percentage, seedling length and dry weight increased as seeds approached maturity. Lipid and protein accumulation in seeds of both species increased progressively with maturity and showed a positive correlation with seed weight accumulation. In both beach pea and grass pea seeds, S6 was identified as a physiological maturity stage.Key words: beach pea, grass pea, hard seed, imbibition, Lathyrus, seed coat, seed development, water impermeability.

scholarly journals Chemical composition of bambara groundnut (V. subterranea L. Verdc) seed parts

2013 ◽  
Vol 48 (3) ◽  
pp. 167-178 ◽  
Author(s):  
AA Olaleye ◽  
EI Adeyeye ◽  
AJ Adesina
Keyword(s):  
Chemical Composition ◽  
Human Health ◽  
Proximate Composition ◽  
Crude Protein ◽  
Dry Weight ◽  
Bambara Groundnut ◽  
Weight Basis ◽  
Crude Fibre ◽  
Mineral Bioavailability ◽  
Good For

The levels of proximate composition, minerals, antinutrients, fibre components and calculated parameters for mineral bioavailability were determined in the testa, dehulled and whole seeds of Bambara groundnut on dry weight basis. Proximate levels were (g/100 g): ash (2.46- 4.36); crude fat (2.47-6.99); crude protein (15.2-22.2); crude fibre (1.03-22.9) and carbohydrate (51.6-61.9). The non-starch polysaccharide (NSP) components were (%): ADF, 7.13-29.0 (or 16.1 %-65.5 %); NDF, 1.77-23.6 (or 6.28 %-83.7 %); ADL, 6.15-28.0 (or 14.9 %-67.8 %); cellulose, 1.36-23.3 (or 5.02 %-86.0 %) and hemicellulose, 0.84-26.5 (or 2.86 %-90.1 %). In minerals (mg/100 g): Mn, Co and Cu were not detected; Na, K, Ca, Mg, Fe and P were low in values whereas Zn was high at 11.2-40.2. These parameters were also good for human health: Na/K (0.47-0.51) and Ca/Mg (2.58-4.36). Antinutrient values showed that Phy was high (14.4-29.2 mg/g); oxalate was high (5.02- 8.59 mg/g) and unavailable phosphorus as Pp % of P (10.2-49.3 %). The mineral bioavailability showed Ca/Phy to be good at 0.20-0.89 and [Ca] [Phy]/[Zn] to be good at 0.09-0.23 thereby making Zn bioavailable in all the samples. DOI: http://dx.doi.org/10.3329/bjsir.v48i3.17325 Bangladesh J. Sci. Ind. Res. 48(3), 167-178, 2013

Anatomical structure and nutritive value of lupin seed coats

2001 ◽  
Vol 52 (10) ◽  
pp. 985 ◽  
Author(s):  
Z. H. Miao ◽  
J. A. Fortune ◽  
J. Gallagher
Keyword(s):  
Seed Coat ◽  
Nutritive Value ◽  
Crude Fibre ◽  
Hard Seed ◽  
Anatomical Analysis ◽  
Lupin Seed ◽  
Series Of Experiments ◽  
Selection For ◽  
Growing Conditions ◽  
Seed Coats

Selection and breeding for yield and adaptation to environmental conditions often changes a number of characteristics of crops, and may influence the value of seed for animals. A series of experiments was conducted to evaluate the effect of breeding and growing conditions on the structure and degradability of lupin seed coats. Breeding has had significant influences on both seed size and seed coat structure of lupins. For instance, cultivars of Lupinus angustifolius released in 1987 and 1988 tended to have smaller seeds with a thicker seed coat than those released in 1971 (P < 0.05). Selection for soft seeds has resulted in a reduction of seed coat thickness in L. angustifolius. Hardseeded and roughseeded lines of L. cosentinii had thicker coats (P < 0.05) than softseeded and smoothseeded, respectively. The main contributor to the thick seed coat of hardseeded lines was a layer of cells known as the hourglass layer, which is located between the outer palisade and inner parenchyma. Anatomical analysis revealed that the soft seed coat tended to have short and round cells, whereas the hard seed tended to have long cells in the palisade layer. Smooth seeds had round cells in the subpalisade, but rough seeds had long cells in this layer. Although the seed coats of lupins contained about 80% crude fibre, with L. cosentinii and L. pilosus having more fibre than L. angustifolius, the fibre in lupin seed coats was highly digestible by sheep.

Dormancy in Pinusmonticola seed related to stratification time, seed coat, and genetics

1987 ◽  
Vol 17 (4) ◽  
pp. 294-298 ◽  
Author(s):  
R. J. Hoff
Keyword(s):  
Seed Coat ◽  
White Pine ◽  
Hard Seed ◽  
Physiological Conditions ◽  
Western White Pine ◽  
Minor Site ◽  
Coat Layer ◽  
A Minor ◽  
Hard Seed Coat

The inner layer of the seed coat, a papery membrane, and physiological conditions of the gametophyte–embryo were shown to be major sites of dormancy in seed of western white pine. The hard seed coat layer was a minor site. With no stratification, 7% of the seeds were not dormant, 9% were dormant because of the hard seed coat, 34% were dormant because of the inner seed coat, and 50% were dormant because of physiological conditions of the gametophyte–embryo. These values varied with stratification times and seed lots (families).

scholarly journals Emergence of `Genesis' Triploid Watermelon following Mechanical Scarification

1999 ◽  
Vol 124 (4) ◽  
pp. 430-432 ◽  
Author(s):  
John R. Duval ◽  
D. Scott NeSmith
Keyword(s):  
Seed Coat ◽  
Citrullus Lanatus ◽  
Crop Improvement ◽  
Coarse Sand ◽  
Financial Risks ◽  
Hard Seed ◽  
Series Of Experiments ◽  
Triploid Watermelon ◽  
Slow Growing ◽  
Hard Seed Coat

Production of triploid watermelon [Citrullus lanatus (Thunb.) Matsum & Nakai] transplants is hindered by poor, inconsistent emergence, and frequent seed coat adherence to cotyledons. Seed coat adherence leads to weakened and slow growing plants. High seed costs, coupled with stand establishment problems, discourages transplant producers from growing this crop. Improvement of triploid watermelon emergence will lessen financial risks to growers and transplant producers and will provide a more reliable production system. Mechanical scarification was evaluated as a means to overcome inconsistent emergence and seed coat adherence. Seeds of `Genesis' triploid watermelon were placed in a cylinder with 100 g of very coarse sand (1.0 to 2.0 mm diameter) and rotated at 60 rpm for 0, 6, 12, 24, and 48 hours in a series of experiments. Number of emerged seed was recorded daily, to obtain emergence dynamics. No significant differences were observed in seed coat adherence among treatments. The longest duration of scarification However, enhanced emergence as compared to the control in three of four experiments. These data support earlier suggestions that a thick or hard seed coat is a factor contributing to poor germination and emergence of triploid watermelons.

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