scholarly journals Field Evaluation of Native B. thuringiensis Isolates Against Aphids (Aphis fabae)

2020 ◽  
Vol 7 ◽  
pp. 115-122
Author(s):  
Ganga GC ◽  
Charu Arjyal
Keyword(s):  
Bacillus Thuringiensis ◽  
Field Evaluation ◽  
Staining Technique ◽  
Selection Method ◽  
Aphis Fabae ◽  
Preliminary Screening ◽  
Before And After ◽  
Phaseolus Species ◽  
Mortality Percentage ◽  
Aphicidal Activity

Objectives: The purpose of this study was to evaluate the aphicidal activity of native Bacillus thuringiensis (Bt) strains. Methods: Soil samples of Provinces 2 and 3 of Nepal were collected randomly for isolation of Bt by acetate selection method. Bt were identified by observing insecticidal crystal proteins (ICPs) by Commassie Brilliant Blue (CBB) staining technique. Aphicidal activity of 12 B. thuringiensis isolates was evaluated by two processes. The preliminary screening was done by spraying the suspension containing the spore and ICPs mixture in Phaseolus species heavily infested with black aphids (Aphis fabae) in fi elds. The second process (selective bioassay) was done by counting the number of aphids (nymphs, instar, winged, wingless) before and after spraying 5ml of suspension containing the spore and ICPs mixture on the leaf or on the beans pods surface infested by Aphids. The mortality percentage of Aphids after treatment was calculated on the 4th day, by counting the live aphids and the result was recorded. Results: Preliminary screening for aphicidal activity revealed that 4 isolates ML5(1), CW1(1), SN2(1) and MP2(1) producing spherical crystal protein, showed 100% mortality against nymphs, instar, winged and wingless Aphids. Isolates were effective in controlling the Aphid (Aphis fabae) within 4 days and the part of the plant that was sprayed becomes free of Aphids. Selective bioassay of native isolate MP3(3) was most effective in killing 95.83% of aphids followed by CW2(1), 85.71%, ML5(1), 77.34%, SN3(1), 72.72%, CW1(1), 70.21%. Conclusion: This study revealed that indigenous Bacillus thuringiensis of Terai region of Nepal are effective in controlling Aphids.  

scholarly journals Pengembangan bahan ajar berbasis muatan lokal pada mata kuliah Pendidikan Kewarganegaraan

2016 ◽  
Vol 13 (2) ◽  
pp. 113-126
Author(s):  
Emil El Faisal ◽  
Sulkipani Sulkipani
Keyword(s):  
Student Achievement ◽  
Civic Education ◽  
Field Evaluation ◽  
Pilot Project ◽  
Local Content ◽  
Before And After ◽  
The Subject ◽  
The Mean ◽  
Potential Impact ◽  
History Department

This research aimed at (1) developing teaching materials based on local content for Civic Education in University; and (2) describing the potential impact of local content on student consciousness on local culture. It was a developmental research. The subject were student registered in Civic Education Course (Mata Kuliah PKn) at History Department. Pilot project was validated through one to one, small group, and field evaluation. The analyzed result indicates that the developed instrument was valid and has potential impact. Supported data shows that the mean score prior to implementation was 6.86 and after implementation was 7.73. In other words, there was an increasing student achievement before and after its implementation. Accordingly, we suggest that this developed material could be implemented widely.

scholarly journals Evaluation of dry beans for resistance to ashy stem blight

1969 ◽  
Vol 74 (4) ◽  
pp. 349-355
Author(s):  
James S. Beaver ◽  
Miguel Martínez ◽  
Graciela Godoy
Keyword(s):  
Field Evaluation ◽  
Growing Season ◽  
Macrophomina Phaseolina ◽  
Percentage Germination ◽  
Dry Beans ◽  
Preliminary Screening ◽  
Spearman Rank ◽  
Inoculation Methods ◽  
Stem Blight ◽  
Seed Inoculation

Experiments were conducted to determine the effectiveness of two inoculation methods in the field evaluation of beans for resistance to ashy stem blight. Seed inoculation resulted in lower germination and greater infection by Macrophomina phaseolina than that in nontreated plots. Stem inoculation did not significantly differ from the control in percentage disease. Least significant differences among genotypes were smaller toward the end of the growing season. Therefore, bean genotypes should be evaluated for ashy stem blight infection near senescence. Spearman rank correlations between percentage germination and percentage infection near senescence were significant in 2 years. Seed inoculation combined with germination tests may be useful in the preliminary screening of bean genotypes for resistance to ashy stem blight.

scholarly journals Laboratory and field evaluation of certain wild plant extracts against Aphis fabae Scop. (Homoptera: Aphididae) and its predators

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Ragab Shaker Abdel-Rahman ◽  
Ismail Abd elkhalek Ismail ◽  
Tarik Abdelhalim Mohamed ◽  
Mohamed Elamir F. Hegazy ◽  
Khaled Abdelhady Abdelshafeek
Keyword(s):  
Plant Extracts ◽  
Field Evaluation ◽  
Aphis Fabae ◽  
Wild Plant

scholarly journals Field evaluation of Fipronil 3G, Fipronil 50 SC and Bacillus thuringiensis H-14 against the dengue vector Aedes albopictus in tires

1997 ◽  
Vol 48 (4) ◽  
pp. 351-354 ◽  
Author(s):  
Sallehudin SULAIMAN ◽  
Zainol Ariffin PAWANCHEE ◽  
Ahmad WAHAB ◽  
Jefri JAMAL ◽  
Abdul Rahim SOHADI ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Albopictus ◽  
Field Evaluation ◽  
Dengue Vector

FIELD EVALUATION OF INSECTICIDES FOR THE CONTROL OF THE EUROPEAN SKIPPER, THYMELICUS LINEOLA (LEPIDOPTERA: HESPERIIDAE), IN QUEBEC

1979 ◽  
Vol 111 (12) ◽  
pp. 1313-1317 ◽  
Author(s):  
Michel Letendre ◽  
Jeremy N. McNeil
Keyword(s):  
Bacillus Thuringiensis ◽  
Larval Mortality ◽  
Field Evaluation ◽  
Field Conditions ◽  
Target Effects

AbstractThe efficacy of seven chemical insecticides, five commercial preparations of Bacillus thuringiensis, and two preparations containing diatom dust were tested against the European skipper, Thymelicus lineola (Ochs.), under field conditions. Satisfactory control was obtained with carbaryl, carbofuran, methomyl, permethrin, trichlorfon, Dipel 45B(=Dipel SC), Dipel WP, Thuricide 26B, Thuricide HPC, and Novabac 3 while dimethoate, chlorpyrifos, and the diatom dusts afforded little or no foliage protection. While larval mortality occurred less rapidly when B. thuringiensis preparations were applied, their use is strongly recommended as they afford the same degree of foliage protection as chemical insecticides and diminish possible undesirable off-target effects.

Chant and Be Happy: The Effects of Chanting on Respiratory Function and General Well-Being in Individuals Diagnosed with Depression

2005 ◽  
Vol 15 (1) ◽  
pp. 61-64
Author(s):  
Molly Kenny ◽  
Raphael Bernier ◽  
Carey DeMartini
Keyword(s):  
Pilot Study ◽  
Beck Depression Inventory ◽  
Respiratory Function ◽  
Effective Means ◽  
Severe Depression ◽  
Extended Period ◽  
Well Being ◽  
Output Level ◽  
Preliminary Screening ◽  
Before And After

The effects of chanting on respiratory function and general well-being in individuals with mild to severe depression were tested in this pilot study. A total of 10 women and 1 man participated in this 8-week-long chanting program. All of the participants had been previously diagnosed with mild to severe depression. Several testing measures were employed. All subjects filled out a preliminary screening questionnaire and took the Beck Depression Inventory at the beginning of the study prior to their first chanting class and at the last meeting before chanting started. A 5-point Likert Scale Questionnaire was administered before and after each session. Spirometry was used to evaluate subjects' FVC, FEV, and FET. Each subject also self-administered pre and post peak/flow tests, taking the best of three forced exhalations before and after each chanting session. At the end of 8 weeks, our results showed that participants increased their breath control and overall expiratory output level. Additionally, 10 of our 11 subjects surveyed showed an increase in their overall mood on the Beck Depression Inventory. The score for one participant remained the same. Preliminary results from this pilot study indicate that chanting is an effective means of increasing people's moods in the immediate present, as well as over an extended period of time in which chanting is performed at least once per week.

Field Evaluation of Transgenic Corn Containing a Bacillus thuringiensis Berliner Insecticidal Protein Gene Against Helicoverpa zea (Lepidoptera: Noctuidae)

1996 ◽  
Vol 31 (3) ◽  
pp. 340-346 ◽  
Author(s):  
Steven R. Sims ◽  
Jay C. Pershing ◽  
Barbara J. Reich
Keyword(s):  
Bacillus Thuringiensis ◽  
Helicoverpa Zea ◽  
Negative Relationship ◽  
Field Evaluation ◽  
Corn Earworm ◽  
Insecticidal Protein ◽  
Transgenic Corn ◽  
First Instar ◽  
Transgenic Lines ◽  
Lepidoptera Noctuidae

Twelve independently transformed lines of transgenic corn (Zea mays L.) expressing the CryIA(b) insecticidal protein from Bacillus thuringiensis var. kurstaki were field tested to evaluate their resistance to the corn earworm, Helicoverpa zea (Boddie). Silks of the primary (=top) ears of transgenic [CryIA(b) positive] and isoline control plants [no CryIA(b) protein] were artificially infested with first-instar H. zea larvae and the length of ear penetration was measured after 19 d. Eight of the 12 lines had significantly less ear damage than their respective isoline controls; 3 transgenic lines reduced H. zea feeding damage by > 75% and stunted surviving H. zea larvae. Concentration of the CryIA(b) protein (μg/g fresh weight) in silks of the transgenic lines, determined using ELISA, ranged from 0.0 to 1.28 μg/g. Within transgenic lines, there was a weak (P < 0.06) negative relationship between the concentration of CryIA(b) protein in fresh silks and the length of H. zea ear penetration.

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