The economics of reducing sulfonamide residues in pork

1992 ◽  
Author(s):  
Virginia Marie Berger
Keyword(s):  
Sulfonamide Residues

scholarly journals Use of chemometric techniques to design a microbiological method for sulfonamide detection in milk

2013 ◽  
Vol 31 (No. 6) ◽  
pp. 627-632 ◽  
Author(s):  
O.G. Nagel ◽  
M.P. Molina ◽  
R.L. Althaus
Keyword(s):  
Experimental Design ◽  
Logistic Model ◽  
Logistic Regression Model ◽  
Culture Medium ◽  
Spore Concentration ◽  
Microbiological Method ◽  
Burman Design ◽  
Detection Capabilities ◽  
Sulfonamide Residues ◽  
Plackett Burman Design

We proposed an experimental design of a microbial bioassay of dichotomous response (positive or negative) using Bacillus subtilis BGA for the detection of sulfonamide residues. In the first stage, the bioassay response time was reduced to 6 h by increasing the spore concentration of B. subtilis. Then, the effects of spore, indicator, trimethoprim (TMP) concentration, and volume of the culture medium were examined with a Plackett Burman design (2<sup>4-1</sup>). Finally, the effect of TMP concentration on the method detection capabilities and specificity was analysed using a logistic model with interaction. The detection capabilities of sulfonamides in milk are close to the MRLs when using 500 mg/l of TMP in the culture medium of the bioassay. It is concluded that the experimental design techniques and a logistic regression model can be used to design successfully a dichotomous response bioassay.

Comparison of Sulfadimethoxine Residue Analyses in Salmon Muscle Using HPLC and Charm II Test

1995 ◽  
Vol 58 (6) ◽  
pp. 678-682 ◽  
Author(s):  
DAVID D. KITTS ◽  
MING ZHENG ◽  
ERIN BURNS-FLETT ◽  
KEITH M. McERLANE
Keyword(s):  
Muscle Tissue ◽  
Chinook Salmon ◽  
High Performance ◽  
Fish Muscle ◽  
Hplc Method ◽  
Screening Strategies ◽  
Antibiotic Screening ◽  
Sulfonamide Residues ◽  
Tissue Matrix ◽  
Good Agreement

Sulfadimethoxine (SDM) residues in chinook salmon muscle were measured by high-performance liquid chromatography (HPLC) and the Charm II test to evaluate the efficacy of the Charm test for the measurement of sulfonamide residues in fish muscle. Both HPLC and Charm analyses were performed in the presence of salmon tissue matrix for the purpose of evaluating the results in field conditions. The sensitivity of the HPLC method was 0.05 μg/g (0.05 ppm) for SDM, while the sensitivity of the Charm II test was at least 0.01 ppm. SDM analysis from salmon fed Romet-30® performed using the Charm assay gave consistently lower values compared to HPLC; however, good agreement was obtained between both methods (r2 = 0.944) over a concentration range of 0.1 to 13.0 ppm in muscle tissue. The large variation in SDM concentrations between fish sampled on common days suggests that a larger number of fish are required for more accurate risk assessment. In the current investigation, the Charm II test was shown to be effective in measuring SDM in salmon muscle tissue for the large sample throughput common with antibiotic-screening strategies.

Gas Chromatographic-Mass Spectrometric Determination of Six Sulfonamide Residues in Egg and Animal Tissues

1990 ◽  
Vol 73 (6) ◽  
pp. 886-892 ◽  
Author(s):  
Keigo Takatsuki ◽  
Tadashi Kikuchi
Keyword(s):  
Methylene Chloride ◽  
Mass Spectrometric ◽  
Gas Chromatography Mass Spectrometry ◽  
Spectrometric Method ◽  
Mass Spectrometric Method ◽  
Animal Tissues ◽  
Selected Ion Monitoring ◽  
Ether Solution ◽  
Sulfonamide Residues

Abstract A gas chromatographlc-mass spectrometric method using selected Ion monitoring mode for simultaneous determination of 6 sulfonamides in egg and edible animal tissues has been developed. Sulfonamides are extracted from a sample with acetonltrlle. The extract Is passed through a silica cartridge column and concentrated. Dlazomethane in ether Is added to methylate sulfonamides. After evaporation, the residue Is dissolved In methylene chloride and cleaned up by silica gel column chromatography. The methylene chloride eluate containing sulfonamide-methyl derivatives Is evaporated to dryness, redlssolved In ether and partitioned between 6N hydrochloric acid. The acid phase is made alkaline, extracted with ether, and the ether solution, after concentration, Is analyzed by gas chromatography-mass spectrometry in selected Ion monitoring mode. Average recoveries from egg and silver salmon fortified at 1 and 0.2 ppm levels with 6 sulfonamides are 99.2 and 84.3%, respectively; coefficients of variation are 7.03 and 11.20%, respectively. Detection limits are 0.01-0.05 ppm.

scholarly journals Planar Chromatography for the Multiclass, Multiresidue Screening of Chloramphenicol, Nitrofuran, and Sulfonamide Residues in Pork and Beef

1997 ◽  
Vol 80 (4) ◽  
pp. 737-740 ◽  
Author(s):  
Jean-Pierre Abjean
Keyword(s):  
High Performance ◽  
Thin Layer Chromatography Plate ◽  
Amino Derivative ◽  
Residue Level ◽  
Long Wave ◽  
Qualitative Detection ◽  
Animal Muscle ◽  
Chromatography Plate ◽  
Sulfonamide Residues ◽  
Layer Chromatography

Abstract A method is described for multiclass and multiresidue qualitative detection of chloramphenicol, nitrofuran, and sulfonamide residues in animal muscle. The drugs are extracted from 1 g tissue with 2 mL ethyl acetate and purified by silica solidphase extraction. After elution of the cartridge, the collected solution is evaporated, and the residue is dissolved in methanol and chromatographed on a Si60 high-performance thin-layer chromatography plate. After evaporation of solvent, nitrofurans are visualized first by their specific UV photochemical reaction with pyridine. Then chloramphenicol is reduced to its amino derivative, and this derivative and the sulfonamides are visualized by long-wave UV after reaction with fluorescamine. Chloramphenicol, nitrofurans, and sulfonamides are detected at residue level of 10, 5, and 100 μg/kg, respectively, or less in pork and beef.

Thin Layer Chromatographic Screening Method for Sulfadiazine Residues in Calf Tissues, Plasma, and Urine

1978 ◽  
Vol 61 (3) ◽  
pp. 545-549
Author(s):  
Joseph L Woolley ◽  
Oliver Murch ◽  
Carl W Sigel
Keyword(s):  
Thin Layer ◽  
Screening Method ◽  
Target Tissue ◽  
Highly Sensitive ◽  
Tissue Homogenates ◽  
Specific Tissue ◽  
Sulfonamide Residues ◽  
Kidney Liver ◽  
Layer Chromatography ◽  
Endogenous Substances

Abstract Because of the lack of specificity of the Bratton-Marshall procedure for assaying sulfonamides, a sensitive, specific tissue residue assay for sulfadiazine (SDZ) was developed. The methodology has been extended to provide a highly sensitive screen for sulfonamide residues, which employs 2-dimensional thin layer chromatography in conjunction with fluorescamine derivatization. The procedure described, which has been developed for SDZ in calf tissues, involves direct ethyl acetate extraction of tissue homogenates. Following evaporation of the organic phase, a portion of the residue is spotted on a 20x20 cm silica gel 60 plate, which is then developed in 2 dimensions with solvent systems devised to separate SDZ from endogenous substances as well as from 12 other sulfonamides that might be present in calf tissues. The presence of SDZ at a concentration of 0.1 ppm or its absence is easily demonstrated in calf kidney, liver, muscle, plasma, and urine. The basic method can be modified for a particular sulfonamide in a target tissue and can be used as a quantitative assay for sulfonamide residues.

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