Use of chemometric techniques to design a microbiological method for sulfonamide detection in milk

We proposed an experimental design of a microbial bioassay of dichotomous response (positive or negative) using Bacillus subtilis BGA for the detection of sulfonamide residues. In the first stage, the bioassay response time was reduced to 6 h by increasing the spore concentration of B. subtilis. Then, the effects of spore, indicator, trimethoprim (TMP) concentration, and volume of the culture medium were examined with a Plackett Burman design (2<sup>4-1</sup>). Finally, the effect of TMP concentration on the method detection capabilities and specificity was analysed using a logistic model with interaction. The detection capabilities of sulfonamides in milk are close to the MRLs when using 500 mg/l of TMP in the culture medium of the bioassay. It is concluded that the experimental design techniques and a logistic regression model can be used to design successfully a dichotomous response bioassay.

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Optimization of Selenium Yeild by Saccharomyces sp. III Using Statistical Designs

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.781-784.1278 ◽

2013 ◽

Vol 781-784 ◽

pp. 1278-1283

Author(s):

Na Xin Sun ◽

Gui Xiang Zhang ◽

Yue Hui Liu ◽

Yuan Xiu Wang

Keyword(s):

Sodium Selenite ◽

Culture Medium ◽

Screening Method ◽

Liquid Volume ◽

Statistical Experimental Design ◽

Box Behnken Design ◽

Burman Design ◽

Statistical Designs ◽

Plackett Burman Design ◽

Dry Cell

The objective of the study was to optimize the conditions in a culture medium for the selenium yeild enriched by Saccharomyces sp. III using Plackett-Burman design and Box-Behnken design. The Plackett-Burman multifactorial design was first employed to screen the significant factors in the fermentation for the selenium yeild, and subsequent use of the response surface methodology was further optimized for the selenium yeild by Box-Behnken design. The important factors in the culture medium, identified by the initial screening method of Placket-Burman, were sodium selenite, glucose and the liquid volume. The optimal amounts for maximum selenium yeild were: sodium selenite 15.8 mg/L; glucose 40.2 g/L; the liquid volume 120 mL in 250 mL flask. Using this statistical experimental design, the selenium yeild under optimal condition reached about 1679.32 μg selenium /g dry cell.

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Optimization of Factorial Design with the type of Plackett-Burman Design to Study the Effects of Organic Rice Production Process: First Step Experiment

International Journal of Advanced Trends in Computer Science and Engineering ◽

10.30534/ijatcse/2021/831032021 ◽

2021 ◽

Vol 10 (3) ◽

pp. 2080-2084

Keyword(s):

Experimental Design ◽

Statistical Model ◽

Production Process ◽

Factorial Design ◽

Process Model ◽

Basic Principles ◽

Organic Rice ◽

Burman Design ◽

Design Type ◽

Plackett Burman Design

The research aims at the optimization to determine factors production process of the of the output organic rice so that the best possible set of parameters affecting it can be selected to get the desired output. For this purpose, the effect of various parameters on the organic rice output is studied. To facilitate the study and detailed analysis, a statistical model is constructed which is used to predict and optimize the performance of the system. Efficient organic rice optimization determines the input variable settings to adjust the organic rice of the organic rice according to the consumption of the parts in the next phase of production process.To study the interaction among the factors a 36 factorial experiment approach has been adopted using the two basic principles of experimental design replication and randomization. The process model was formulated based on analysis of variance (ANOVA) and regression of coefficient using Minitab Release 19.00. The output is represented graphically and in the form of empirical model which defines the performance characteristics of the production process of organic rice by factorial design type of Placket-Burman design

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Optimization of protease production by the fungusMonacrosporium thaumasium and its action againstAngiostrongylus vasorum larvae

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612013000200048 ◽

2013 ◽

Vol 22 (2) ◽

pp. 285-288 ◽

Cited By ~ 4

Author(s):

Filippe Elias de Freitas Soares ◽

Fabio Ribeiro Braga ◽

Jackson Victor de Araújo ◽

Walter dos Santos Lima ◽

Lanuze Rose Mozzer ◽

...

Keyword(s):

Larvicidal Activity ◽

Culture Medium ◽

Nematophagous Fungus ◽

Protease Production ◽

Control Groups ◽

Vitro Assay ◽

Burman Design ◽

The Mean ◽

Plackett Burman Design

The objectives of this study were to optimize protease production from the nematophagous fungus Monacrosporium thaumasium (NF34a) and evaluate its larvicidal activity and biological stability. An isolate of the nematophagous fungus Monacrosporium thaumasium (NF34a) was used to produce the enzyme. The Plackett-Burman design was used in order to scan which components of the culture medium could have a significant influence on protease production by the fungus NF34a. An in vitro assay was also performed to evaluate the larvicidal activity of NF34a. It was observed that only one component of the culture medium (yeast extract), at the levels studied, had any significant effect (p < 0.05) on protease production. There was a reduction (p < 0.01) in the mean number of larvae recovered from the treated groups, compared with the control groups. The results confirm previous reports on the efficiency of nematophagous fungi for controlling nematode larvae that are potentially zoonotic. Thus, given the importance of biological control, we suggest that further studies should be conducted on the protease produced by the fungus Monacrosporium thaumasium.

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