Antimicrobial resistance and ability to form biofilm of coagulase-negative staphylococci isolated from touch surfaces in Polish hospital wards

Linezolid is currently used to treat infections caused by multidrug-resistant Gram-positive cocci. Both linezolid-resistant S. aureus (LRSA) and coagulase-negative staphylococci (CoNS) strains have been collected worldwide. Two isolates carrying linezolid resistance genes were recovered from laryngological patients and characterized by determining their antimicrobial resistance patterns and using molecular methods such as spa typing, MLST, SCCmec typing, detection of virulence genes and ica operon expression, and analysis of antimicrobial resistance determinants. Both isolates were multidrug resistant, including resistance to methicillin. The S. aureus strain was identified as ST-398/t4474/SCCmec IVe, harboring adhesin, hemolysin genes, and the ica operon. The S. haemolyticus strain was identified as ST-42/mecA-positive and harbored hemolysin genes. Linezolid resistance in S. aureus strain was associated with the mutations in the ribosomal proteins L3 and L4, and in S. haemolyticus, resistance was associated with the presence of cfr gene. Moreover, S. aureus strain harbored optrA and poxtA genes. We identified the first case of staphylococci carrying linezolid resistance genes from patients with chronic sinusitis in Poland. Since both S. aureus and CoNS are the most common etiological factors in laryngological infections, monitoring of such infections combined with surveillance and infection prevention programs is important to decrease the number of linezolid-resistant staphylococcal strains.

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Antimicrobial resistance profiles of microorganisms isolated from hospitalized patients in Dominican Republic

Revista Panamericana de Salud Pública ◽

10.26633/rpsp.2020.36 ◽

2020 ◽

Vol 44 ◽

pp. 1

Author(s):

David de Luna ◽

José Javier Sánchez ◽

Miguel Peguero ◽

Wilmary García ◽

Sylmari Liciaga ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Dominican Republic ◽

Methicillin Resistance ◽

Carbapenem Resistance ◽

Generation Cephalosporin ◽

Cross Sectional Study ◽

Hospitalized Patients ◽

Coagulase Negative Staphylococci ◽

Cross Sectional ◽

Acinetobacter Spp

Objective. To define the antimicrobial resistance profiles of the microorganisms most commonly isolated from hospitalized adult patients in Dominican Republic (DR). Methods. A retrospective, cross-sectional study of phenotypic antimicrobial susceptibility patterns was conducted using data from 3 802 clinical microbiology reports specifying positive bacterial cultures in samples collected from patients admitted to the clinical, surgery, and intensive care units (ICU) at three tertiary-level care hospitals in the city of Santiago de los Caballeros from 1 January 2016 – 31 December 2017. Descriptive statistics and chi-square test (P ≤ 0.05) were used to analyze the qualitative variables. Results. At the three hospitals, there were 932, 1 090, and 1 780 microbiology reports analyzed. Of the total, 1274 were from the ICU, 1 042 from the surgery unit, and 1 486 from the clinical unit. Methicillin resistance was found in 57.3% of the Staphylococcus aureus isolates and 75.3% of the coagulase-negative staphylococci. Third-generation cephalosporin resistance was detected in 54.4% of isolates identified as members of the Enterobacteriaceae family, 67.3% of the Acinetobacter spp., and 91.7% of the Pseudomonas, while carbapenem resistance was shown by 8.0%, 23.8%, and 51.0% of these, respectively. Most of the resistant Acinetobacter spp. isolates were found in just one hospital and the prevalence of Enterobacteriaceae resistant to carbapenems was highest in the ICU. Conclusion. Antimicrobial resistance levels are high among hospitalized patients in Dominican Republic and may cause enhanced risk factors that impact clinical outcomes. Urgent measures are needed to address antimicrobial resistance in DR.

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Antimicrobial Resistance in Coagulase-Negative Staphylococci

Journal of Medical Microbiology ◽

10.1099/00222615-19-2-217 ◽

1985 ◽

Vol 19 (2) ◽

pp. 217-226 ◽

Cited By ~ 26

Author(s):

J. M. T. Hamilton-Miller ◽

A. Iliffe

Keyword(s):

Antimicrobial Resistance ◽

Coagulase Negative Staphylococci

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Identification of hemolysin encoding genes and their association with antimicrobial resistance pattern among clinical isolates of coagulase-negative Staphylococci

BMC Research Notes ◽

10.1186/s13104-020-4938-0 ◽

2020 ◽

Vol 13 (1) ◽

Cited By ~ 2

Author(s):

Mona Nasaj ◽

Zahra Saeidi ◽

Babak Asghari ◽

Ghodratollah Roshanaei ◽

Mohammad Reza Arabestani

Keyword(s):

Antimicrobial Resistance ◽

Clinical Isolates ◽

Resistance Pattern ◽

Coagulase Negative Staphylococci ◽

Encoding Genes

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Environmental organisms from different hospital wards

British Journal of Infection Control ◽

10.1177/175717740200300405 ◽

2002 ◽

Vol 3 (4) ◽

pp. 10-14 ◽

Cited By ~ 5

Author(s):

S Dancer ◽

J Raeside ◽

M Boothma

Keyword(s):

The Elderly ◽

Antibiotic Consumption ◽

Coagulase Negative Staphylococci ◽

Chi Square ◽

Gram Negative ◽

Defined Daily Doses ◽

High Dependency ◽

Significant Difference ◽

Bacillus Spp ◽

Hospital Wards

T hree clinical wards in the same hospital, High Dependency (HDU), Care of the Elderly (CE) and Acute Psychiatry (AP), were screened for environmental organisms. The screening programme ran for four weeks and targeted comparable sites from all three units. Floor areas were similar, as were staffing levels, cleaning schedules, infection control policies and patient numbers. Organisms such as coagulase-negative staphylococci, Bacillus spp., coliforms, oxidase-positive Gram-negative bacilli, Clostridium difficile, enterococci, various fungi and Staphylococcus aureus were isolated. Staphylococci and Gram-negative bacilli were tested against clinically appropriate antibiotics. There was little variation in diversity or density of organisms from any of the wards, except for significant differences in antibiotic susceptibilities of the organisms (P<0.0001 HDU v AP, P=0.0057 CE v AP and P=0.0365 HDU v CE). From HDU, 49% (of 43) isolates were resistant to four or more antibiotics and from CE, 37% (of 54) isolates were resistant to four or more. From AP, just 2% (of 52) were resistant to four or more antibiotics. 9% HDU organisms were fully susceptible, as compared with 20% of those from CE and 27% from AP. Antibiotic data (in Defined Daily Doses (g)/100 bed-days) showed that HDU consumed over 12 times more antibiotics than CE, which in turn consumed twice as much as AP; these were mostly intravenous broad-spectrum agents for HDU, as opposed to oral preparations for the other two wards (Chi-square for each ward for linear trends by level of antibiotic intake were all P<0.0001). It was concluded that the only significant difference between environmental bacteria from wards of varying specialities in this hospital is their resistance to antibiotics. Heavy use of antibiotics in a hospital unit, as demonstrated by antibiotic consumption data, may be associated with increased antibiotic resistance in environmental organisms originating from that unit.

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Antimicrobial resistance and genetic characterization of coagulase-negative staphylococci from bovine mastitis milk samples in Korea

Journal of Dairy Science ◽

10.3168/jds.2019-17028 ◽

2019 ◽

Vol 102 (12) ◽

pp. 11439-11448 ◽

Cited By ~ 2

Author(s):

Su-Jeong Kim ◽

Dong Chan Moon ◽

Seung-Chun Park ◽

Hee Young Kang ◽

Seok Hyeon Na ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Genetic Characterization ◽

Bovine Mastitis ◽

Coagulase Negative Staphylococci ◽

Milk Samples

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Presence and antimicrobial resistance of coagulase-negative staphylococci isolated from animals in a Veterinary Teaching Hospital in Cyprus

Veterinární Medicína ◽

10.17221/166/2019-vetmed ◽

2020 ◽

Vol 65 (No. 5) ◽

pp. 191-198

Author(s):

H Sukur ◽

OM Esendal

Keyword(s):

Antimicrobial Resistance ◽

Teaching Hospital ◽

Treatment Options ◽

Companion Animals ◽

Clinical Importance ◽

Scientific Data ◽

Multiple Drug ◽

Negative Effects ◽

Coagulase Negative Staphylococci ◽

Resistance Patterns

Coagulase-negative staphylococci (CoNS) are a group of commensal microorganisms residing on the skin and mucous membranes of both humans and animals. Until recently, they have been regarded as non-pathogenic to livestock and companion animals, but since then, their clinical importance in veterinary medicine has increased with the discovery of their potential pathogenic roles in animals causing skin and soft tissue infections together with spontaneous abortions and mastitis. Scientific data concerning the presence of CoNS in North Cyprus are very limited. Therefore, the purpose of the study reported herein was to investigate the presence and antimicrobial resistance patterns of CoNS species isolated from various animals presented at the Veterinary Teaching Hospital in North Cyprus between July 2018 and 2019. Staphylococci were isolated from 37.0% (87/235) of the samples submitted, within which 60.9% (53/87) and 39.1% (34/87) were identified as coagulase-positive staphylococci (CoPS) and coagulase-negative staphylococci (CoNS), respectively. Among the CoNS, S. chromogenes was the most predominantly isolated species (14/34, 41.2%), followed by S. capitis (5/34, 14.7%) and S. simulans (4/34, 11.8%). Of these 34 CoNS, 24 (70.6%) and 10 (29.4%) were identified as MRCoNS and MSCoNS, respectively. The CoNS isolates showed relatively high levels of resistance towards amoxicillin/clavulanic acid (19/34, 55.9%), tetracycline (14/34, 41.2%) and penicillin (13/34, 38.2%). In conclusion, the presence of CoNS, especially MRCoNS, and the detection of multiple drug resistant (MDR) species with a high prevalence were regarded as being important since they might limit and have negative effects on the therapeutic treatment options of staphylococcal infections in animals, and might have both public and veterinary concerns.

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