scholarly journals Identification of Important Rice Cultivars in Akita Prefecture with One Grain by STS-PCR Method.

2000 ◽  
Vol 47 (8) ◽  
pp. 632-637 ◽  
Author(s):  
Hironobu OGASAWARA ◽  
Saori TAKAHASHI
Keyword(s):  
Rice Cultivars ◽  
Akita Prefecture ◽  
Pcr Method

scholarly journals Identification of Glutinous Rice Cultivars Using Rice Cake as Samples by the PCR Method.

2001 ◽  
Vol 48 (4) ◽  
pp. 306-310 ◽  
Author(s):  
Ken'ichi OHTSUBO ◽  
Sumiko NAKAMURA ◽  
Koichi YOZA ◽  
Koichi SHISHIDO
Keyword(s):  
Rice Cultivars ◽  
Rice Cake ◽  
Glutinous Rice ◽  
Pcr Method

scholarly journals Cultivar Identification and Palatability Estimation of 14 Typical Philippine Rice Cultivars by the PCR Method

2006 ◽  
Vol 53 (12) ◽  
pp. 634-643 ◽  
Author(s):  
Sumiko Nakamura ◽  
Keitaro Suzuki ◽  
Kazutomo Haraguchi ◽  
Yoko Takemoto ◽  
Bienvenido O. Juliano ◽  
...  
Keyword(s):  
Cultivar Identification ◽  
Rice Cultivars ◽  
Pcr Method

scholarly journals Differentiation of Sake Material Rice Cultivars by PCR Method

2007 ◽  
Vol 54 (5) ◽  
pp. 233-236 ◽  
Author(s):  
Sumiko Nakamura ◽  
Keitaro Suzuki ◽  
Kazutomo Haraguchi ◽  
Ken'ichi Ohtsubo
Keyword(s):  
Rice Cultivars ◽  
Pcr Method

Investigation of duck plague virus in hoar areas of Bangladesh

2020 ◽  
Vol 26 (1-2) ◽  
pp. 73-78
Author(s):  
A Hossen ◽  
MH Rahman ◽  
MZ Ali ◽  
MA Yousuf ◽  
MZ Hassan ◽  
...  
Keyword(s):  
Cytopathic Effect ◽  
Clinical Sample ◽  
Chorioallantoic Membrane ◽  
Clinical Samples ◽  
Isolation And Identification ◽  
Duck Plague Virus ◽  
Pcr Method ◽  
Polymerase Chain ◽  
The Individual ◽  
Free Ranging

Duck plague (DP) is the most important infectious disease of geese, ducks and free-ranging water birds. The present study was conducted to determine the prevalence of duck plague virus followed by isolation and identification. For these purposes, a total of 155 cloacal swabs samples were collected randomly from duck of different haor areas of Bangladesh including 45 (41 surveillance and 4 clinical) samples from Netrokona; 42 (40 surveillance and 2 clinical) samples from Kishoregonj; 30 samples from Brahmanbaria and 38 samples from Sunamganj. The samples were processed and pooled (1:5 ratio) for initial screening of target polymerase gene of duck plague virus by polymerase chain reaction (PCR) method. All the samples of a positive pool were then tested individually for identifying the individual positive samples. The result showed that out of 155 samples, 41 (26.45%) were found positive in which 17 were from Netrokona, where 15 (36.58%) were from surveillance samples and 2 (50%) were from clinical sample; 16 were from Kishoregonj, where 14 (35%) were from surveillance samples and 2 (100%) were from clinical sample; 2 (6.6%) were from Brahmanbaria and 5 (13.15%) were from Sunamganj. These positive samples were inoculated into 9-10 days embryonated duck eggs (EDE) through chorioallantoic membrane (CAM) route for the isolation of virus. The EDE died earlier was also chilled, and in a similar way, the CAMs were collected and again performed PCR for id entification of virus. Out of 41 PCR positive samples, 26 samples were isolated and reconfirmed by PCR. Subsequently, DPV was isolated in primary duck embryo fibroblasts cell culture and confirmed by observing cytopathic effect (CPE). Bang. J. Livs. Res. Vol. 26 (1&2), 2019: P. 73-78

USING DNA MARKERS ENCODING P24 AND GP51 PROTEINS IN PCR-RT TO INCREASE SENSITIVITY AND SPECIFICITY OF THE PCR METHOD FOR INDICATION OF BLV

2019 ◽  
pp. 8-14
Author(s):  
R.F. Safina ◽  
◽  
G.R. Lukmanova ◽  
K.V. Usoltsev ◽  
N.I. Khammadov ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Dna Markers ◽  
Pcr Method

Diagnosis of helicobacter pylori infection: problems and prospects

2020 ◽  
pp. 54-59
Author(s):  
A. S. Molostova ◽  
N. S. Gladyshev ◽  
A. V. Svarval ◽  
R. S. Ferman ◽  
A. B. Karasyova ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Urease Activity ◽  
Diagnostic Methods ◽  
Study Group ◽  
Biochemical Method ◽  
Antimicrobial Drugs ◽  
Non Invasive ◽  
Number Of Patients ◽  
Pcr Method ◽  
Positive Results

(HP) infection was performed using invasive and non-invasive methods. The study group consisted of 95 patients with dyspepsia. HP infection was detected in 47 patients (49.4 %). The expediency of using a set of diagnostic methods for detecting HP (PCR, immunochromatographic, bacteriological and method for determining urease activity) is proved. Most often (100 %) in patients HP infection was detected in biopsies using the PCR method. Somewhat less frequently it was detected when examining biopsies with an invasive biochemical method (AMA RUT Reader) (82 %) and fecal immunochromatographic method (83 %). Despite the fact that helicobacteriosis was detected bacteriologically in a small number of patients (24 %), this method is of particular value, since it allows you to assess the sensitivity to antimicrobial drugs and probiotics, and does not give false positive results.

WEEDS IN POTATO CULTURE AND THEIR OUTCOME IN SPREADING OF Alternaria spp.

2018 ◽  
Vol 17 (6) ◽  
pp. 159-166
Author(s):  
Halina Kurzawińska ◽  
Stanisław Mazur ◽  
Małgorzata Nadziakiewicz ◽  
Jacek Nawrocki
Keyword(s):  
Chenopodium Album ◽  
Potato Cultivar ◽  
Cirsium Arvense ◽  
Potato Plants ◽  
Potato Culture ◽  
Rapd Pcr ◽  
Microscopic Features ◽  
Weed Infestation ◽  
Pcr Method ◽  
Potato Crops

The aim of this study was to determine whether the weeds accompanying potato crops can be a source of Alternaria spp. causing Alternaria leaf blight and to determine the genetic similarities of Alternaria alternata isolates infecting selected weeds: Chenopodium album, Cirsium arvense and tested potato cultivar. Three-year field experiment was conducted on the potato cultivar ‘Vineta N’. The isolates were classified into different species on the basis of macro- and microscopic features. In each year of the study, A. alternata dominated among the isolated fungi colonizing the leaves of potato plants and the selected weeds. The genetic similarities of A. alternata isolates was determined by the RAPD-PCR method. Tested genetic forms of A. alternata were closely related; only small differences in the pattern of the separated amplification products was evidenced. The dominance of A. alternata on the weeds accompanying potato crops suggests that if weed infestation is extensive, the pathogen is very likely to spread and its population to increase.

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