scholarly journals Morphological potential of the gene pool of radish

2020 ◽  
Vol 1 (97) ◽  
pp. 260-268
Author(s):  
O.P. Ovchinnikova
Keyword(s):  
Raphanus Sativus ◽  
Quantitative Traits ◽  
Geographical Origin ◽  
Genetic Material ◽  
Root Crop ◽  
Raphanus Sativus L ◽  
Quantitative Characteristics ◽  
Red Variety ◽  
Efficiency Of Selection ◽  
Selection Of

The article presents the research of samples of the collection material of radish sowing Raphanus sativus L. Ukrainian and foreign selection of different ecological and geographical origin. Weather conditions during the research period (2015–2017) were characterized by a high variability of hydrothermal conditions, which manifested itself in the lack of moisture at different stages of ontogenesis of the.radish. Subsequently, the uneven amount of precipitation during the growing season led to a decrease in yield and a change in the biometric indicators of the rosette of leaves and root crops. As you know, varieties of radish Raphanus sativus L. are complex populations with a variety of genetic material, especially in terms of quantitative characteristics. The quantitative traits of a plant organism are subject to strong modification variability, which is the main obstacle to increasing the efficiency of selection of the required selection genotypes. One of the criteria for the variability of quantitative traits is the coefficient of variation (V, %). This statistical indicator allows one to obtain information about the characteristics of the reaction rate of various quantitative traits of a plant, while ensuring the comparability of the results obtained. The conducted studies of biometric indicators of sowing radish made it possible to analyze the variation of its main quantitative characteristics. In the course of the research, a description of the variability of the morphological characteristics of the samples of sowing radish of various breeding and geographical origin was carried out. The revealed features make it possible to analyze the nature of variability of quantitative traits and contribute to the widespread involvement of the best varieties in the breeding process as donors of valuable traits. According to the research results, it was allocated for mechanized harvesting on the basis of the «smallest rosette of leaves» varieties of sowing radish: 18 days (Russia) a variety of pink-red with a white tip and a cylindrical root crop, Zarya (Russia) — a dark red variety with a rounded root crop, Duet (Italy) is a pink-red variety with an oval root crop. And also varieties White cylindrical and Ice icicle — a variety of white cylindrical Ukrainian selection. During the study, it was found that the size of the root crop is controlled by the genotype, but its formation is more dependent on external environmental factors. For selection according to the parameter «length of the root crop», the following samples were selected: 18 days (Russia) and White brekfast (Germany), according to the «width of the root crop» — Belaya (Czech Republic), Zhara (Netherlands)

MANIFESTATION OF TRANSGRESSION BY MAIN QUANTITATIVE CHARACTERISTICS OF WINTER TRITICAL PRODUCTIVITY IN F2 HYBRID POPULATIONS

2021 ◽  
Vol 1 ◽  
pp. 3-7
Author(s):  
В. Д. Тромсюк ◽  
◽  
В. Д. Бугайов ◽  
Keyword(s):  
Maximum Degree ◽  
Geographical Origin ◽  
Selection Process ◽  
Diallel Analysis ◽  
Grain Weight ◽  
Maximum Frequency ◽  
Winter Triticale ◽  
Research Material ◽  
Quantitative Characteristics ◽  
Selection Of

The research was conducted in 2014-2017 in the department of selection of fodder, grain ears, and industrial crops in the fields of scientific crop rotation of the Institute of Fodder and Agriculture of Podillya NAAS of Ukraine. A collection of 114 hexaploid specimens of different ecological and geographical origins was used as research material. Six varieties of winter triticale of different ecological and geographical origin and the manifestation of valuable economic features were used in crosses according to the scheme of full diallel analysis: Polovetske (UA0602494), Amos (UA0602627) originating from Ukraine; Kapryz (UA0601781), Tsekad 90 (UA0602066) - Russia; Dubrava (UA0602222) - Belarus and Pawo (UA0602555) - Poland. Only the best plants, which did not lag in growth and were normally developed, were selected for hybridization. The obtained generation F0 was sown in 2016, and generation F1 – 2017 manually in an experimental field with the same depth, which provides plants with the same area of nutrition. After full maturation, the plants were also collected manually in sheaves, structural analysis was performed on the main indicators of productivity. The frequency and degree of manifestation of positive transgressions in hybrids of the second generation (F2) of winter triticale were evaluated based on productivity: productive bushiness, number and weight of ear grains and grain weight from the plant. The best hybrids by degree and frequency of manifestation of positive transgression are selected. The degree of transgressions in productive bushiness averaged 65.3%, frequency - 43%; number of grains in the main ear - 7.4%, frequency - 15.3%; weight of grain in the ear - 13.8% at a frequency of 27, 7%; weight of grain from the plant - 91.5% with a transgression frequency of 52.7%. The best hybrid combinations of winter triticale on the basis of "productive bushiness" - Polovtsian / Caprice and Dubrava / Polovtsian (Tc = 100%), the maximum frequency of transgressions on this basis - hybrids Polovtsian / Caprice / Pawos and Pawo are selected and offered for use in the selection process. TC = 60); "Number of grains from the ear" - the maximum degree of transgressions was found in the hybrid Pawo / Polovtsian (Tc = 18.1%), the maximum frequency - in the hybrid Caprich / Pawo (Tch = 40); "Ear grain mass" - Pawo / Amos (Tc = 37.0%), the highest frequency of transgressions - in hybrids Amos / Dubrava and Kaprikh / Pawo (Tch = 50); "Grain weight from the plant" - Polovtsian / Amos (Tc = 37.0%), the frequency of transgressions - hybrids Polovtsian / Amos and Polovtsian / Caprice (Tc = 85%).

scholarly journals Korelasi Genotipe dan Fenotipe Antar Sifat Kuantitatif Pada Populasi Segregasi Transgresif Kacang Hijau

2020 ◽  
Vol 16 (1) ◽  
pp. 21-30
Author(s):  
Julian Timisela ◽  
Aca A Anakotta ◽  
Adriana Hiariej ◽  
Edizon Jambormias
Keyword(s):  
Vigna Radiata ◽  
Variance Components ◽  
Quantitative Traits ◽  
Genetic Material ◽  
Transgressive Segregation ◽  
Food Plants ◽  
Direct Selection ◽  
Selection Of ◽  
Genotype Correlation ◽  
Selection Generation

Mung bean (Vigna radiata L. Wilczek) is one of the food plants as a source of vegetable protein for humans. One of these is a population of transgressive segregation resulting from crossing in mung beans. This research aims to estimate the correlation between genotype and phenotype values ​​between quantitative traits in the transgressive segregation population of mung beans. Genetic material was 11 lines of transgressive segregated in the selection generation S2:2 of varieties Mamasa Lere Butnem × Lasafu Lere Butnem crosses. Genotype correlation analysis was obtained from the decomposition of genetic variance components from the results of the analysis of variance according to a randomized complete design with sub-sampling, consisting of 13 genotypes (including check varieties) which were two replications. The results showed a genotype correlation between the quantitative traits of mung beans that indicated the possibility of direct selection of the populations of transgressive segregation lines on yields and indirectly through easily observable traits such as harvest age, number of pods, and number of seeds. Keywords: genetic correlation, mungbeans, quantitative traits   ABSTRAK Kacang hijau (Vigna radiata L. Wilczek) merupakan salah satu tanaman pangan sebagai sumber protein nabati bagi manusia. Salah satu diantaranya adalah populasi segregasi transgresif hasil persilangan pada kacang hijau. Penelitian ini bertujuan untuk menduga nilai korelasi genotipe dan fenotipe antar sifat-sifat kuantitatif pada populasi segregasi transgresif kacang hijau. Bahan genetik adalah 11 galur segregan transgresif pada generasi seleksi S2:2 zuriat persilangan varietas Mamasa Lere Butnem × Lasafu Lere Butnem. Analisis korelasi genotipe diperoleh dari penguraian komponen ragam-peragam genetik dari hasil analisis peragam sesuai model acak rancangan acak lengkap dengan anak contoh, terdiri atas 13 genotipe (termasuk varietas penguji) yang diulang dua kali. Hasil penelitian memperlihatkan adanya korelasi genotipe antar sifat-sifat kuantitatif kacang hijau yang mengindikasikan kemungkinan seleksi langsung populasi galur segregasi transgresif terhadap hasil maupun tak-langsung melalui sifat yang mudah diamati seperti umur panen, jumlah polong dan jumlah biji. Kata Kunci: kacang hijau, korelasi genetik, sifat kuantitatif

scholarly journals The effect of temperature on growth and development of cultivars of radish (Raphanus sativus L. var. radicula Pers.) under summer conditions.

1978 ◽  
Vol 26 (1) ◽  
pp. 68-75
Author(s):  
M. Nieuwhof
Keyword(s):  
Root Growth ◽  
Raphanus Sativus ◽  
Dry Matter ◽  
Cultivar Differences ◽  
Effect Of Temperature ◽  
Root Weight ◽  
Matter Production ◽  
Raphanus Sativus L ◽  
Maximum Root ◽  
Selection Of

During 1977, seven cultivars and strains were sown in glasshouses at temperatures of 10, 14, 17, 20, 23 and 26 deg C and harvested at five dates. Maximum leaf and dry-matter production occurred at 20 and 23 deg C, and maximum root growth initially at 20 and 23 deg C, but later at 17 deg C. Cultivar differences in rate of leaf and root growth were observed, and cultivar X environment interactions were apparent for leaf and root growth under summer conditions. The overall order of cultivars for leaf and root weight was similar to that for plants grown under autumn conditions [see PBA 47, 5915]; hence it is suggested that selection of plants for growing in winter could be performed during the summer. (Abstract retrieved from CAB Abstracts by CABI’s permission)

Development of mutant RsF3′H allele-based marker for selection of purple and red root in radish (Raphanus sativus L. var. longipinnatus L. H. Bailey)

Euphytica ◽  
2019 ◽  
Vol 215 (7) ◽  
Author(s):  
Takanori Masukawa ◽  
Kyeong-Seong Cheon ◽  
Daiki Mizuta ◽  
Masayuki Kadowaki ◽  
Akira Nakatsuka ◽  
...  
Keyword(s):  
Raphanus Sativus ◽  
Raphanus Sativus L ◽  
Selection Of

Correlation of morphological traits with molecular markers in radish (Raphanus sativus)

2004 ◽  
Vol 44 (8) ◽  
pp. 813 ◽  
Author(s):  
A. Pradhan ◽  
G. Yan ◽  
J. A. Plummer
Keyword(s):  
Molecular Markers ◽  
Raphanus Sativus ◽  
Morphological Traits ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Morphological Characters ◽  
Marker Assisted Breeding ◽  
Seedling Length ◽  
Raphanus Sativus L ◽  
Selection Of

Radish (Raphanus sativus L.) is an important vegetable crop in Asia. Lack of uniformity in the crop at harvest is due to genetic variability and environmental conditions. Molecular markers associated with morphological traits of seed and seedlings were identified. Seed (100) of radish cvv. Fire Ball and Long White Chinese from Australia and Mino Early, Pyuthane Red, Tokinasi, White Neck and 40 Days from Nepal. The seed was germinated between paper (20°C, 6 days) and seedlings were grown in pots under glasshouse conditions (20°C, further 3 weeks). Morphological characters were measured in seed and seedlings at 6 days and 4 weeks after germination. Deoxyribonucleic acid was extracted from selected samples and Random Amplified Polymorphic DNA (RAPD) markers were identified using 10 primers. Multivariate analysis based on principle coordinates analysis was used to correlate morphological traits with molecular markers within and across cultivars. Several markers associated with high or low seed weight, germination proportion, seedling length and fresh weight were identified. This new method for identifying potential RAPD markers may be useful for marker assisted breeding and selection of improved radish varieties.

scholarly journals Variability of the effects of combining ability in the breeding of F1 radish hybrids

2021 ◽  
Vol 845 (1) ◽  
pp. 012024
Author(s):  
A A Mironov ◽  
A A Ushanov ◽  
A V Voronina ◽  
M V Vorobyev
Keyword(s):  
Combining Ability ◽  
Quantitative Traits ◽  
Root Crop ◽  
Economically Valuable Traits ◽  
Stable Performance ◽  
Consistent Performance ◽  
Relationship Of ◽  
The Relationship ◽  
Second Period ◽  
Selection Of

Abstract Based on homozygous lines, three sterile and seven fertile, radishes were crossed by the method of two groups of genotypes. The resulting offspring and parental lines were tested when growing in soil, in a film greenhouse, using traditional techniques. The experiment was repeated twice to identify hybrid combinations showing consistent performance. The grown plants were evaluated according to economically valuable traits: the weight of the root crop and the aboveground vegetative part, the length, and diameter of the root crop. In the method proposed by V.K. Savchenko, statistical indicators of the combining ability of lines were calculated, including the effects of GCA. When tested at different times, we identified lines that changed the direction of the action of genes on the manifestation of quantitative traits. For the selection of lines for future breeding programs, lines were selected that showed stable performance. The test took the indicators of the inbred lines themselves, which made it possible to assess the relationship of the phenotypic manifestation of the trait with the effects of GCA of the same lines. The presence of close relationships allows simplifying the work of breeders, namely, to assess the manifestation of a trait in plants, without evaluating the offspring. For the same purpose, the presence of relationships between quantitative signs was assessed; during the test, close correlations were found in the first period, while in the second period they were absent. The data obtained indicate the unreliability of even close correlations obtained from the results of single tests. The indicators of the GCA effects of the studied lines are applicable for the selection of only a specific breeding institution, while the comparison of indicators for two or more test periods helps to identify patterns in the control of the inheritance of the studied quantitative traits.

Isolation of the constituents and evaluation of allelopathic potential of Raphanus sativus L. (Brassicaceae)

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
LLR Fiorucci ◽  
GC Mantovanelli ◽  
DI Bernardi ◽  
AA Silva ◽  
RS de Oliveira Jr ◽  
...  
Keyword(s):  
Raphanus Sativus ◽  
Allelopathic Potential ◽  
Raphanus Sativus L

Combination of ribosome and phage display for fast selection of high affinity VHH antibody fragments

2019 ◽  
pp. 27-33
Author(s):  
YuE Kravchenko ◽  
SV Ivanov ◽  
DS Kravchenko ◽  
EI Frolova ◽  
SP Chumakov
Keyword(s):  
Phage Display ◽  
Selection Procedure ◽  
Free System ◽  
Phage Displays ◽  
High Affinity ◽  
Binding Domains ◽  
A Cell ◽  
Vhh Antibodies ◽  
Efficiency Of Selection ◽  
Selection Of

Selection of antibodies using phage display involves the preliminary cloning of the repertoire of sequences encoding antigen-binding domains into phagemid, which is considered the bottleneck of the method, limiting the resulting diversity of libraries and leading to the loss of poorly represented variants before the start of the selection procedure. Selection in cell-free conditions using a ribosomal display is devoid from this drawback, however is highly sensitive to PCR artifacts and the RNase contamination. The aim of the study was to test the efficiency of a combination of both methods, including pre-selection in a cell-free system to enrich the source library, followed by cloning and final selection using phage display. This approach may eliminate the shortcomings of each method and increase the efficiency of selection. For selection, alpaca VHH antibody sequences suitable for building an immune library were used due to the lack of VL domains. Analysis of immune libraries from the genes of the VH3, VHH3 and VH4 families showed that the VHH antibodies share in the VH3 and VH4 gene groups is insignificant, and selection from the combined library is less effective than from the VHH3 family of sequences. We found that the combination of ribosomal and phage displays leads to a higher enrichment of high-affinity fragments and avoids the loss of the original diversity during cloning. The combined method allowed us to obtain a greater number of different high-affinity sequences, and all the tested VHH fragments were able to specifically recognize the target, including the total protein extracts of cell cultures.

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