Expression Of Gamma Glutamyl Hydrolase And Carbonic Anhydrase 9 In Oral Squamous Cell Carcinoma And Their Association With Extracapsular Spread

Introduction: Extracapsular spread (ECS) is one of the most important prognostic factors in oral squamous cell carcinoma (OSCC). Gamma Glutamyl Hydrolase (GGH) is a lysosomal enzyme which is involved in folate homeostasis. It is overexpressed in several human malignancies but its role in OSCC has never been reported. Carbonic Anhydrase 9 (CA9) is a transmembrane glycoprotein and is related to hypoxia. High expression of CA9 has been associated with poor prognosis in several tumours including OSCC. This study was aimed to investigate the expression of GGH and CA9 in OSCC and their potential use as biomarkers to predict ECS. Materials and Methods: Immunohistochemical staining with GGH and CA9 markers were performed in 35 cases of OSCC (19 with ECS and 16 without ECS) and 5 cases of normal mucosa. A semi-quantative index was used for immunoscoring. Association between the expression of these markers and ECS status were analyzed using chi-square test. Results: Immunohistochemical results indicated that GGH and CA9 were upregulated in OSCC. High GGH and CA9 expression were significantly associated with ECS (p<0.05). Combination of GGH and CA9 showed greater prognostic accuracy as compared to the individual markers. Conclusion(s): Both separately and in combination GGH and CA9 offer potential as prognostic biomarkers in OSCC and was thus reliable in segregating the OSCC cases based on ECS status.

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P237 The expression of carbonic anhydrase 9 (CA9) is associated with postoperative recurrence and poor prognosis in surgically treated oral squamous cell carcinoma

Oral Oncology Supplement ◽

10.1016/s1744-7895(07)70514-x ◽

2007 ◽

Vol 2 (1) ◽

pp. 202

Author(s):

S.W. Choi ◽

J.Y. Park ◽

J.H. Shin ◽

S. Lee ◽

I.H. Cha ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Carbonic Anhydrase ◽

Cell Carcinoma ◽

Squamous Cell ◽

Poor Prognosis ◽

Postoperative Recurrence ◽

Carbonic Anhydrase 9

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Relationship Between mir15b and Sal-Like Protein 4 and Biological Behavior of Oral Squamous Cell Carcinoma

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2021.2548 ◽

2021 ◽

Vol 11 (2) ◽

pp. 308-314

Author(s):

Zengbo Wu ◽

Yan Yan ◽

Xianzhuo Chen ◽

Yanling Liu ◽

Dinggen Chen

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Normal Mucosa ◽

Clinicopathological Features ◽

Biological Behavior ◽

Control Group ◽

Tumor Tissues ◽

Proliferation And Invasion

miR15b and SALL4 are involved in a variety of tumor progression. The roles of miR15b and SALL4 in oral squamous cell carcinoma (OSCC) remains unclear. The tumors and normal mucosa of OSCC patients were collected to detect miR15b and SALL4 level by Real-time PCR and analyze their correlation with OSCC clinicopathological features. Oral cancer Tca8113 cells were separated into control group; miR15b mimics group and miR15b inhibitor group followed by analysis of SALL4 expression, cell survival by MTT assay; cell invasion by Transwell chamber assay, as well as expression of N-cadherin and Vimentin and correlated with TNM stage, tumor volume and metastasis, and positively with differentiation TGF-β by Western blot. miR15b expression was decreased and SALL4 expression was increased in OSCC tumor tissues. miR15b was negatively degree (P < 0.05), whereas, opposite correlation of SALL4 with the above parameters was found (P < 0.05). miR15b and SALL4 were negatively correlated. MiR15b mimics significantly up-regulated MiR15b, decreased SALL4 expression, inhibited Tca8113 cell proliferation and invasion, as well as reduced N-cadherin, Vimentin and TGF-βexpression (P < 0.05). Opposite results were found in MiR15b inhibitor group. MiR15b expression is decreased and SALL 4 is increased in OSCC tumor tissues. MiR15b and SALL4 is closely related to OSCC clinicopathological features. MiR15b regulates the expression of EMT-related genes and TGF-β, thereby altering the proliferation and invasion of OSCC cells.

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Expression of Extracellular Matrix—Laminin in Oral Squamous Cell Carcinoma: An Immunohistochemical Study

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1120 ◽

2012 ◽

Vol 13 (2) ◽

pp. 194-200

Author(s):

Sonam C Kapse ◽

Ajit V Koshy ◽

Nirmala N Rao ◽

Sushant S Kamat ◽

Kamal Kiswani ◽

...

Keyword(s):

Extracellular Matrix ◽

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Immunohistochemical Study ◽

Tumor Development ◽

Treatment Strategies ◽

Prognostic Indicators ◽

Chi Square

ABSTRACT Aim To evaluate the expression of laminin in various grades of oral squamous cell carcinoma (OSCC) in order to determine whether this protein can be used as a marker for early detection and elucidation of oral cancer. Materials and methods Immunohistochemical staining for laminin was done on 60 selected archival blocks of histopathologically diagnosed cases of primary OSCC and the laminin expression was compared between the different histopathological grades of primary OSCC. The statistical analysis was performed by using Chi-square (÷ square) test and Gaussiantest with a probability of p < 0.05 was considered as significant. Results It was observed that laminin expression decreased with tumor progression which may be correlated to the tumor aggressiveness. Conclusion There was a gradual decrease of laminin staining with decreasing cellular differentiation, with differentiated lesions showing a more conspicuous staining of basement membrane glycoprotein than less differentiated lesions. Clinical significance An understanding of how the extracellular matrix influences tumor development and invasion is fundamental in the development of new prognostic indicators and treatment strategies for oral squamous cell carcinoma. How to cite this article Koshy AV, Rao NN, Kamat SS, Kiswani K, Kapse SC, Shaikh NA. Expression of Extracellular Matrix— Laminin in Oral Squamous Cell Carcinoma: An Immunohistochemical Study. J Contemp Dent Pract 2012;13(2):194-200.

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Survivin Expression in Metastatic and Nonmetastatic Oral Squamous Cell Carcinoma: A Comparative Study

Journal of Advanced Oral Research ◽

10.1177/2320206820968448 ◽

2020 ◽

pp. 232020682096844

Author(s):

B. Sekar ◽

K. Indrapriyadharshini ◽

M. Ambika ◽

R. Saranyan ◽

Madhavan Nirmal ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Survivin Expression ◽

Staining Intensity ◽

Fetal Tissue ◽

Chi Square ◽

Multifunctional Protein ◽

Chi Square Test

Aim: Survivin is a multifunctional protein chiefly involved in apoptosis and cell cycle regulation. Increased expression of survivin in tumors and fetal tissue determines its antiapoptotic activity. The aim of the study is to identify the immunoexpression of survivin in metastatic and nonmetastatic oral squamous cell carcinoma (OSCC) and also to evaluate and compare the expression of survivin in metastatic and nonmetastatic OSCC of buccal mucosa. Materials and Methods: In total, 40 histopathologically proven cases of OSCC, including 20 metastatic and 20 nonmetastatic cases, are selected. Among the 20 metastatic and nonmetastatic cases, 10 well-differentiated and 10 moderately differentiated squamous cell carcinoma cases were included and were subjected to immunohistochemical staining for survivin expression. The results were analyzed by SPSS version 11.5 using chi-square test. Results: The expression of survivin in metastatic and nonmetastatic tumors is 15%–70% and 15%–60%, respectively. When comparing the cases of moderately differentiated squamous cell carcinoma in metastatic and nonmetastatic tumors, 70% cases show moderate staining intensity. Conclusion: The survivin expression was comparatively high in metastatic OSCC. Also based on the aforementioned results, survivin expression was high in increasing grades of OSCC.

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NOTCH1 mutations and extracapsular spread in oral squamous cell carcinoma lymph node metastasis

European Journal of Surgical Oncology ◽

10.1016/j.ejso.2016.07.061 ◽

2016 ◽

Vol 42 (11) ◽

pp. S230

Author(s):

Priyanka Bhattacharya ◽

Janet Risk ◽

Jagjit Dhanda ◽

Richard Shaw ◽

Bryony Lloyd ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Extracapsular Spread ◽

Node Metastasis ◽

Notch1 Mutations

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Expression of RAD51 and Its Clinical Impact in Oral Squamous Cell Carcinoma

Analytical Cellular Pathology ◽

10.1155/2020/1827676 ◽

2020 ◽

Vol 2020 ◽

pp. 1-6

Author(s):

Yuyang Li ◽

Jia Li ◽

Jingchun Sun ◽

Yingkun Liu ◽

Dingkun Liu ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Normal Skin ◽

Clinical Stage ◽

Prognostic Indicator ◽

Normal Mucosa ◽

Strong Positive Correlation ◽

Tissue Samples

Purpose. To examine the expression of RAD51 in oral squamous cell carcinoma (OSCC) and analyze its connection with pathological grade, clinical stage, and lymphatic metastasis potential. Methods. For this study, 74 OSCC samples, 15 normal mucosa tissues, and 11 normal skin tissue samples were collected. RAD51 expression was investigated using immunohistochemistry. A follow-up visit was used to assess the prognosis of each patient. We compared RAD51 expression in oral mucosa epithelial cells (OMECs), keratinocytes, and tongue squamous cell carcinoma cells (TSCCs) by Western blot analysis. Results. RAD51 expression was higher in tumor cells than in normal mucosal tissues. In addition, RAD51 expression was associated with higher tumor differentiation (P<0.05). Also, RAD51 expression was higher (P<0.05) in patients with lymphatic metastases, and relapse rates were also higher in patients with elevated RAD51 levels (P=0.052). In addition, RAD51 expression levels were highest in the skin keratinocytes, followed by the TSCCs and OMECs. Conclusion. A strong positive correlation was found between RAD51 expression and the degree of malignancy in OSCC patients, suggesting that RAD51 could be an excellent prognostic indicator for OSCC patients.

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