Interaction of Hematological Analysis and α-globin Genotypes among Eligible Blood Donors

2020 ◽  
Vol 19 (3) ◽  
Author(s):  
Lai Kuan Teh ◽  
Li Fang Lim ◽  
Yu Leong Teh ◽  
Elizabeth George ◽  
TzeYan Lee ◽  
...  
Keyword(s):  
Blood Count ◽  
Blood Donors ◽  
Full Blood Count ◽  
Haematological Parameter ◽  
Molecular Characterisation ◽  
Globin Chain ◽  
Significant Difference ◽  
Hematological Analysis ◽  
Alpha Thalassaemia ◽  
Rule Out

Introduction: Alpha thalassaemia is one of the haemoglobin disorders in which the carriers of alpha thalassaemia may have normal haemoglobin level and are eligible to donate blood which may bring complications. This study is to investigate the interaction of haematological parameter with α-globin genotypes among eligible blood donors. Materials & Methods: A cohort study with 270 eligible blood donors were analysed for red cell indices. Alpha-globin (α-globin) genotyping was performed for seven deletions, six point mutations and two triplications. Statistical analyses were performed to compare the α-globin genotypes with haematological data. Results: High prevalence of α-thalassaemia carriers (7.7%, 21/270) was found among blood donors. All of them did not show anaemic pictures with a normal Hb level (>12 gm/dl). Five genotypes were identified consisting of 249 αα/αα (92.2%), nine -α 3.7/αα (3.3%), nine-- SEA/αα (3.3%), two -α 4.2/αα (0.7%) and one ααCS/αα (0.4%). Different α-globin genotypes showed a significant difference in RBC, MCV, MCH, MCHC, RDW, and Hct/Hb ratio (p<0.05) due to the different extent of α-globin chain reduction. Conclusion: Our study concluded that by using Hb level alone is not sufficient to screen for the eligibility of blood donors. Full blood count (FBC) screening with borderline MCV and MCH levels might be able to rule out silent α-thalassemia carriers. FBC and molecular characterisation should be incorporated together to properly rule out α-thalassaemia carriers.><0.05) due to the different extent of α-globin chain reduction. Conclusion: Our study concluded that by using Hb level alone is not sufficient to screen for the eligibility of blood donors. Full blood count (FBC) screening with borderline MCV and MCH levels might be able to rule out silent α-thalassemia carriers. FBC and molecular characterisation should be incorporated together to properly rule out α-thalassaemia carriers.

scholarly journals Can Haematological Indices Predict Positive Findings at Endoscopy in Anaemic Patients?

2007 ◽  
Vol 89 (3) ◽  
pp. 221-225
Author(s):  
AMP Schizas ◽  
R Reid ◽  
ML George
Keyword(s):  
Cell Volume ◽  
Blood Cells ◽  
Blood Count ◽  
Haematological Parameters ◽  
Full Blood Count ◽  
Mean Cell Volume ◽  
Upper Gi ◽  
Significant Difference ◽  
Significant Pathology ◽  
Haematological Indices

INTRODUCTION Patients with anaemia are commonly referred for bidirectional endoscopy. The aim of this study was to determine if any haematological parameters could predict positive findings at endoscopy. PATIENTS AND METHODS A total of 209 patients had bidirectional endoscopies performed for anaemia between September 2002 and March 2004. The endoscopy reports, histology and full blood count results (haemoglobin [Hb], red blood cells [RBCs], packed cell volume [PCV], mean cell volume [MCV] and mean cell haemoglobin [MCH]) were then reviewed. Statistical analysis was performed using non-parametric tests. RESULTS Overall, 197 patients had successful bidirectional endoscopies with 12 requiring completion barium enema. In 48 (23%) of these patients, a cause of anaemia was found with 15 (7.2%) carcinomas detected (2 upper GI and 13 lower GI). There was a significant difference in haemoglobin (9.2 g/dl versus 10.1 g/dl; P = 0.0044), RBCs (3.56 × 1012/l versus 3.83 × 1012/l; P = 0.0325) and PCV (0.279 l/l versus 0.31 l/l; P = 0.0112) between patients with positive findings at endoscopy and those with a normal investigation. Cancer patients had significantly lower haemoglobin (8.65 g/dl versus 10.1 g/dl; P = 0.0103), RBCs (3.45 × 1012/l versus 3.83 × 1012/l; P = 0.0179) and PCV (0.27 l/l versus 0.31 l/l; P = 0.0298) compared with patients with normal endoscopies. There was no significant difference in the other haematological parameters between those found to have positive findings and those that had normal endoscopies. CONCLUSIONS Based on this study, the yield of bidirectional endoscopy is low, with haemoglobin and PCV being the most useful haematological indices of significant pathology. Ferritin and MCV did not predict the likelihood of finding a gastrointestinal cause for the anaemia.

scholarly journals The use of peripheral blood cells as an assessment of inflammation in prostate cancer in patients attending in surgery department at ESUT teaching hospital, Parklane, Enugu, Nigeria

2020 ◽  
Vol 9 (1) ◽  
pp. 1
Author(s):  
Clara N. Soronnadi ◽  
Nancy C. Ibeh ◽  
Francis O. Ugwene ◽  
Grace I. Amilo ◽  
Anthony J. Ede
Keyword(s):  
Prostate Cancer ◽  
Blood Cells ◽  
Blood Count ◽  
Age Groups ◽  
Post Treatment ◽  
Full Blood Count ◽  
Treatment Results ◽  
Lymphocyte Ratio ◽  
Significant Difference ◽  
Pre Treatment

Background: Full blood count (FBC) is a prerequisite investigation requested from all prostate cancer (PCa) patients pre and post treatment, poor parameter influences the outcome of cancers.Methods: Total subjects consisted of 84 male subjects between the ages 41 to >80 years. Longitudinal study was conducted. Controls and test samples were collected at diagnosis and at different stages of the treatment. Demographic information was obtained using a questionnaire. The data was analyzed using IBM statistical package for social sciences (SPSS) PC, version 20.0; SPSS Inc., Chicago, III., USA; the receiver operating characteristic curve (ROC) curve was obtained via neutrophil-to-lymphocyte ratio (NLR), lymphocyte-to-monocyte ratio (LMR) and platelet-to-lymphocyte ratio (PLR) ratios cut-off determinations. Cox proportional-hazards regression analyses the prognostic factors (duration, ratios) and overall survival (diagnosis to death or last follow-up). A paired sample t-test compared test of significance in pre/post treatment results. The analysis of variance (ANOVA) and Tukey HSD post-hoc, test susceptibility within age groups was done.Results: Increased NLR and LMR were significantly associated with increased hazard ratio (HR) and OS at p<0.05 while PLR, no significant difference at P>0.05 in PCa. In complete blood count (CBC) and erythrocytic sedimentation rate (ESR), control and treatment period, all red blood cell (RBC) parameters showed a significant decrease at p<0.05 in treatment results compared to the pre-treatment results while total platelet (TPLT), total white blood cells (TWBC), NC, LC, ESR showed significant increase at p<0.05 in treatment results compared to pre-treatment results. Age group 41-50 years showed more susceptibility than other age groups with significant decrease at p<0.05 in NC, LC and increased MC.Conclusions: This study supports CBC and ESR biomarkers as a prognostic tool in early detection, treatment and monitoring of disease progression in these subjects.

scholarly journals Prevalence of Iron Deficiency, Iron Deficiency Anaemia and General Anaemia in Male Gambian Blood Donors Residing in Greater Banjul Region

2021 ◽  
Author(s):  
Mustapha Dibbasey ◽  
Bolarinde Lawal ◽  
Solomon Umukoro ◽  
Peter Mitchel
Keyword(s):  
Iron Deficiency ◽  
Blood Donor ◽  
Iron Deficiency Anaemia ◽  
Blood Count ◽  
Blood Donors ◽  
Blood Donation ◽  
Full Blood Count ◽  
Serum Samples ◽  
Deficiency Anaemia ◽  
Donor Population

AbstractObjectiveThe objective of this study is to determine the prevalence of iron deficiency (ID) and iron deficiency anaemia (IDA) as well as general anaemia in male blood donors and their association with ageing process.Methodology and ResultsA total of two hundred and one (201) serum samples were analysed for ferritin in male Gambian blood donors. The ferritin measurement was achieved with COBAS® INTEGRA 400 plus. At the same time, haemoglobin values were retrospectively obtained from the archived haematological full blood count result in the GARIS database. IDA was defined as (Haemoglobin <13.0g/dL+ Ferritin<15ng/ml) whilst ID was defined as (Haemoglobin ≥13.0g/dL+ Ferritin<15ng/ml) and general anaemia was defined as haemoglobin <13.0g/dL in males. The prevalence of anaemia (20%, n=41), ID (22%, n=44) and IDA (10%, n=21), were recorded in male donors. The results show no relationship between ferritin and haemoglobin among the blood donors (collection coefficient (r) = 0.04). Besides, no linear association of having anaemia and ID with ageing was reported among the blood donor population.Conclusion and potential application of findingsID and IDA as well as general anaemia are highly prevalent among blood donors in the Gambia. Besides, no predisposition to ID and anaemia was observed in term of age, thus all blood donors from 18-60 should be considered for blood donation without any age preference.

scholarly journals Neutrophil to Lymphocyte ratio versus Monocyte to Lymphocyte ratio in predicting hypertensive diseases of pregnancy

2021 ◽  
Vol 1 (1) ◽  
pp. 8-17
Author(s):  
Moses Banyeh
Keyword(s):  
Pregnant Women ◽  
Blood Count ◽  
Area Under The Curve ◽  
Positive Likelihood Ratio ◽  
Predictive Ability ◽  
Neutrophil To Lymphocyte Ratio ◽  
Full Blood Count ◽  
Lymphocyte Ratio ◽  
Significant Difference ◽  
Hypertensive Diseases

Background: The neutrophil to lymphocyte ratio (NLR) and the monocyte to lymphocyte ratio (MLR) are two systemic inflammatory indices with promising prognostic and predictive abilities for HDP. The study aimed to determine the abilities of the NLR and MLR in predicting HDP among pregnant women in Ghana. Methods: This was a case-control study that was carried out between September 2015 and May 2016 at the Bolgatanga regional hospital. The study involved 50 pregnant women of whom 60% (30/50) had normotensive pregnancies (controls) and 40% (20/50) were confirmed to have HDP (cases). The cases were compared with the controls in terms of their socio-demographic characteristics, full blood count parameters, NLR and MLR. Probability value <0.05 was considered statistically significant. Results: The chances of developing HDP is more likely when lymphocyte count is increased [OR:1.126(95%CI:1.028-1.233)] but less likely with increased NLR [OR: 0.776(95%CI:0.651-0.926)] and MLR [OR: 0.039(95%CI: 0.003-0.469)]. There was no significant difference in the area under the curve (AUC) between NLR and MLR (0.77 vs 0.76, p>0.05). The sensitivities of NLR and MLR were 95.0% and 70.0%, while their specificities were 56.7% and 73.3%, respectively. The positive likelihood ratio (+LR) of MLR was higher than that of NLR (2.6 vs. 2.2). Conclusion: Both the NLR and MLR have moderate predictive ability for hypertensive diseases of pregnancy (HDP). However, the MLR will be a better predictor for HDP than the NLR. We recommend the addition of NLR and MLR when reporting full blood count results for pregnant women.

F.VIII R:Ag in Hepatitis

1979 ◽  
Author(s):  
R. Kotitschke ◽  
J. Scharrer
Keyword(s):  
Chronic Hepatitis ◽  
Blood Donors ◽  
Blood Donation ◽  
Normal Population ◽  
Rabbit Antiserum ◽  
Two Dimensional ◽  
Plastic Plate ◽  
Significant Difference ◽  
Acute Hepatitis B ◽  
The One

F.VIII R:Ag was determined by quantitative immunelectrophoresis (I.E.) with a prefabricated system. The prefabricated system consists of a monospecific f.VIII rabbit antiserum in agarose on a plastic plate for the one and two dimensional immunelectrophoresis. The lognormal distribution of the f.VIII R:Ag concentration in the normal population was confirmed (for n=70 the f.VIII R:Ag in % of normal is = 95.4 ± 31.9). Among the normal population there was no significant difference between blood donors (one blood donation in 8 weeks; for n=43 the f.VIII R:Ag in % of normal is = 95.9 ± 34.0) and non blood donors (n=27;f.VIII R:Ag = 94.6 ± 28.4 %). The f.VIII R:Ag concentration in acute hepatitis B ranged from normal to raised values (for n=10, a factor of 1.8 times of normal was found) and was normal again after health recovery (n=10, the factor was 1.0). in chronic hepatitis the f.VIII R:Ag concentration was raised in the majority of the cases (for n=10, the factor was 3.8). Out of 22 carrier sera 20 showed reduced, 2 elevated levels of the f.VIII R:Ag concentration. in 5 sera no f.VIII R:Ag could be demonstrated. The f.VIII R:Ag concentration was normal for n=10, reduced for n=20 and elevated for n=6 in non A-non B hepatitis (n=36). Contrary to results found in the literature no difference in the electrophoretic mobility of the f.VIII R:Ag was found between hepatitis patients sera and normal sera.

scholarly journals Can Hematological Parameters Play a Role in the Differential Diagnosis of Adrenal Tumors?

Uro ◽  
2021 ◽  
Vol 1 (2) ◽  
pp. 39-44
Author(s):  
Mehmet Gürkan Arıkan ◽  
Göktan Altuğ Öz ◽  
Nur Gülce İşkan ◽  
Necdet Süt ◽  
İlkan Yüksel ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Inflammatory Markers ◽  
Blood Count ◽  
Complete Blood Count ◽  
Low Cost ◽  
Easy Access ◽  
Adrenal Tumors ◽  
Tumor Diameter ◽  
Lymphocyte Ratio ◽  
Significant Difference

There have been few studies reported with conflicting results in the use of neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR), redcell-distribution-width (RDW), etc. for predicting prognosis and differential diagnosis of adrenal tumors. The aim of this study is to investigate the role of inflammatory markers through a complete blood count, which is an easy access low-cost method, for the differential diagnosis of adrenocortical adenoma (ACA), adrenocortical carcinoma (ACC), and pheochromocytoma. The data of patients who underwent adrenalectomy between the years of 2010–2020 were retrospectively analyzed. Systemic hematologic inflammatory markers based on a complete blood count such as neutrophil ratio (NR), lymphocyte ratio (LR), NLR, PLR, RDW, mean platelet volume (MPV), and maximum tumor diameter (MTD) were compared between the groups. A statistically significant difference was found between the three groups in terms of PLR, RDW, and MTD. With post-hoc tests, a statistically significant difference was found in PLR and MTD between the ACA and ACC groups. A statistically significant difference was found between the ACA and pheochromocytoma groups in PLR and RDW values. In conclusion, it could be possible to plan a more accurate medical and surgical approach using PLR and RDW, which are easily calculated through an easy access low-cost method such as a complete blood count, together with MTD in the differential diagnosis of ACC, ACA, and pheochromocytoma.

Full blood count as an ancillary test to support the diagnosis of giant cell arteritis

2018 ◽  
Vol 48 (4) ◽  
pp. 408-413 ◽  
Author(s):  
Lawrence J. Oh ◽  
Eugene Wong ◽  
Juliana Andrici ◽  
Peter McCluskey ◽  
James E. H. Smith ◽  
...  
Keyword(s):  
Giant Cell Arteritis ◽  
Giant Cell ◽  
Blood Count ◽  
Full Blood Count ◽  
Ancillary Test

Morphological and molecular characterisation of Aporcelaimellus simplex (Thorne & Swanger, 1936) Loof & Coomans, 1970 and a new concept for Aporcella Andrássy, 2002 (Dorylaimida: Aporcelaimidae)

Nematology ◽  
2013 ◽  
Vol 15 (2) ◽  
pp. 165-178 ◽  
Author(s):  
Sergio Álvarez-Ortega ◽  
Sergei A. Subbotin ◽  
Reyes Peña-Santiago
Keyword(s):  
Molecular Data ◽  
Evolutionary Tree ◽  
Valid Species ◽  
Molecular Characterisation ◽  
New Combinations ◽  
Terminal Portion ◽  
Significant Difference ◽  
European Populations ◽  
Emended Diagnosis

This contribution presents a study of Iberian and Californian populations of Aporcelaimellus simplex, including morphological, morphometric and molecular data. The species is characterised and distinguished by its lip region offset by an expansion, pars refringens vaginae absent, and conical tail with a small but distinct hyaline terminal portion. No significant difference exists between American and European populations. Molecular data and the derived evolutionary tree show a topology in which this species forms a well-supported group with members of Discolaiminae, far from other representatives of Aporcelaimellus. Putting special emphasis in the absence of pars refringens vaginae, A. simplex is transferred to the genus Aporcella. The taxonomy of this genus is revised in depth, with the proposal of an emended diagnosis, the provision of a list of 13 valid species (mostly new combinations from Aporcelaimellus) as well as a key to their identification and a compendium of their main morphometrics. Aporcella debruinae sp. n. is proposed for Aporcelaimellus papillatus apud de Bruin & Heyns, 1992.

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