Effect of Zebularine in Comparison to and in Combination with Trichostatin A on CIP/KIP Family (p21Cip1/Waf1/Sdi1, p27Kip1, and p57Kip2), DNMTs (DNMT1, DNMT3a, and DNMT3b), Class I HDACs (HDACs 1, 2, 3) and Class II HDACs (HDACs 4, 5, 6) Gene Expression, Cell Growth Inhibition and Apoptosis Induction in Colon Cancer LS 174T Cell Line

Background: Dysregulation of the cell cycle has been reported in various cancers. Inactivation of the cyclin-dependent kinases inhibitors (CDKIs), CIP/KIP family, such as p21Cip1/Waf1/Sdi1, p27Kip1, and p57Kip2 genes because of hypermethylation has been shown in several cancers. Treatment with DNA demethylating agent 5-aza-2ˈ-deoxycytidine (5-Aza-CdR) has been indicated that affect genomic methylation and resulting in silenced genes reactivation in colon cancer. Previously, we evaluated the effect of 5-Aza-CdR on DNA methyltransferase 1 (DNMT1) gene expression in hepatocellular carcinoma (HCC) which encouraged us to design the current study. The present study aimed to evaluate the effect of 5-Aza-CdR on p21Cip1/Waf1/Sdi1, p27Kip1, p57Kip2, and DNAT1 genes expression, cell growth inhibition and apoptosis induction in colon cancer SW 480 and SW 948 cell lines. Materials and Methods: The effect of 5-aza-CdR on the SW 480 and SW 948 cells growth, apoptosis induction and genes expression were assessed by MTT assay, flow cytometry, and real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis respectively. Results: 5-aza-CdR inhibited cell growth as time- and dose-dependent manner significantly (P<0.001). The agent reactivated p15INK4, p16INK4, p18INK4, and p19INK4 genes expression and induced apoptosis at a concentration of 5 μM significantly. Besides, 5-aza-CdR had a more significant effect on the SW 480 cell line in comparison to SW 948 cell line. Conclusion: 5-Aza-CdR plays a key role in the up-regulation of p21Cip1/Waf1/Sdi1, p27Kip1, and p57Kip2 and down-regulation of DNMT1 genes resulting in cell growth inhibition and apoptosis induction. [GMJ.2020;9:e1899] DOI:10.31661/gmj.v9i0.1899

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Investigation of the effect of 5-Aza-2′-deoxycytidine on p15INK4, p16INK4, p18INK4, and p19INK4 genes expression, cell growth inhibition, and apoptosis induction in hepatocellular carcinoma PLC/PRF/5 cell line

Advanced Biomedical Research ◽

10.4103/abr.abr_68_20 ◽

2020 ◽

Vol 9 (1) ◽

pp. 33

Author(s):

Fraidoon Kavoosi ◽

Masumeh Sanaei ◽

Ali Ghasemi

Keyword(s):

Hepatocellular Carcinoma ◽

Cell Growth ◽

Growth Inhibition ◽

Cell Line ◽

Apoptosis Induction ◽

Cell Growth Inhibition ◽

Genes Expression

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EFFECT OF VALPROIC ACID IN COMPARISON WITH VORINOSTAT ON CELL GROWTH INHIBITION AND APOPTOSIS INDUCTION IN THE HUMAN COLON CANCER SW48 CELLS IN VITRO

Experimental Oncology ◽

10.31768/2312-8852.2018.40(2):95-100 ◽

2018 ◽

Vol 40 (2) ◽

pp. 95-100 ◽

Cited By ~ 2

Author(s):

M Sanaei ◽

F Kavoosi ◽

O Mansoori

Keyword(s):

Colon Cancer ◽

Valproic Acid ◽

Cell Growth ◽

Growth Inhibition ◽

Histone Deacetylases ◽

Regulation Of Gene Expression ◽

Human Colon ◽

Apoptosis Induction ◽

Cell Growth Inhibition ◽

Human Colon Cancer

Aim: Acetylation levels of histones are the result of the balance between histone acetyltransfrases and histone deacetylases activities, which plays an important role in chromatin remodeling and regulation of gene expression. Histone deacetylases inhibitors such as valproic acid, vorinostat have attracted interest because of their ability to induce differentiation and apoptosis of cancer cells. The current study was designed to assess the effect of valproic acid in comparison to and in combination with vorinostat on cell growth inhibition and apoptosis induction in the human colon cancer SW48 cells. Materials and Methods: The colon cancer SW48 cells were seeded and treated with various doses of valproic acid and vorinostat and MTT assay and flow cytometric assay were done to determine cell viability and cell apoptosis, respectively. Results: All concentrations of both agents reduced viability significantly in a dose- and time-dependent fashion (p < 0.004). Both compounds, either single or combined agents, induced apoptosis significantly, whereas the ratio of the apoptotic cells treated with combined agents was more significant than the single. Conclusion: Our findings suggest that vaproic acid and vorinostat can significantly inhibit cell growth and induce apoptosis in colon cancer SW48 cells.

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Effect of Curcumin in Comparison with Trichostatin A on the Reactivation of Estrogen Receptor Alpha gene Expression, Cell Growth Inhibition and Apoptosis Induction in Hepatocellular Carcinoma Hepa 1-6 Cell lLine

Asian Pacific Journal of Cancer Prevention ◽

10.31557/apjcp.2020.21.4.1045 ◽

2020 ◽

Vol 21 (4) ◽

pp. 1045-1050

Author(s):

Masumeh Sanaei ◽

Fraidoon Kavoosi ◽

Mehrnoosh Arabloo

Keyword(s):

Gene Expression ◽

Hepatocellular Carcinoma ◽

Estrogen Receptor ◽

Cell Growth ◽

Estrogen Receptor Alpha ◽

Trichostatin A ◽

Apoptosis Induction ◽

Cell Growth Inhibition ◽

Estrogen Receptor Alpha Gene ◽

Alpha Gene

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In VivoAnticancer Activity ofBasella albaLeaf and Seed Extracts against Ehrlich's Ascites Carcinoma (EAC) Cell Line

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2018/1537896 ◽

2018 ◽

Vol 2018 ◽

pp. 1-11 ◽

Cited By ~ 2

Author(s):

Md. Shihabul Islam ◽

Md. Sifat Rahi ◽

Chowdhury Arif Jahangir ◽

Md Habibur Rahman ◽

Israt Jerin ◽

...

Keyword(s):

Side Effects ◽

Cell Growth ◽

Growth Inhibition ◽

Cell Line ◽

Seed Extract ◽

Morphological Alteration ◽

Pcr Analysis ◽

Apoptosis Induction ◽

Cell Growth Inhibition ◽

Seed Extracts

Cancer is a class of diseases characterized by uncontrolled cell growth. The current treatment options of cancer are radiotherapy, chemotherapy, hormone therapy, and surgery, where all of them have unpleasant side effects. Due to their adverse side effects, it is challenging to develop new drug for cancer treatment. Hence, the scientists are trying to seek for noble compounds from natural sources to treat cancer. Therefore, in the present investigation, a widely consumable vegetableBasella albawas subjected to evaluate its antiproliferative effect along with molecular signaling of apoptosis in Ehrlich ascites carcinoma (EAC) cell line. Cell growth inhibition was determined by haemocytometer whereas apoptosis of cancer cells were studied by florescence microscope using Hoechst-33342 stain and result was supported by DNA fragmentation and certain cancer related genes expression through PCR analysis.B. albaleaf and seed extract exhibit a considerable scavenging activity in comparison to a standard antioxidant BHT. Moreover, the leaf and seed extracts were able to agglutinate 2% RBC of goat blood at minimum 12.5μg/ml and 50.0μg/ml concentration, respectively. A significant cytotoxic activity was also found in both leaf and seed extract. In haemocytometic observation, the leaf and seed extracts exhibit about 62.54±2.41% and 53.96±2.34% cell growth inhibition, respectively, whereas standard anticancer drug Bleomycin showed 79.43±1.92% growth inhibition. Morphological alteration under fluorescence microscope showed nuclear condensation and fragmentation which is the sign of apoptosis. Apoptosis induction was also confirmed by DNA laddering in leaf and seed treated EAC cells. Upregulation of the tumor suppressor gene P53and downregulation of antiapoptotic gene Bcl-2 enumerate apoptosis induction. Therefore, current study manifested that leaf and seed extracts ofB. albahave antiproliferative activity against EAC cell line and can be a potent source of anticancer agents to treat cancer.

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