scholarly journals Antioxidant effects of antioxidant biofactor on reactive oxygen species in human gingival fibroblasts

2011 ◽  
Vol 48 (3) ◽  
pp. 209-213 ◽  
Author(s):  
Satoshi Matsui ◽  
Yasuhisa Tsujimoto ◽  
Toshihiko Ozawa ◽  
Kiyoshi Matsushima
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Human Gingival Fibroblasts ◽  
Oxygen Species ◽  
Gingival Fibroblasts ◽  
Antioxidant Effects

PRDX6 Alleviates Lipopolysaccharide-induced Inflammation in Human Gingival Fibroblasts by Regulation of NRF2

2021 ◽  
Author(s):  
Wen-ying Yang ◽  
Xiang Meng ◽  
Yue-rong Wang ◽  
Qing-qing Wang ◽  
Xin He ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Phospholipase A2 ◽  
Inflammatory Cytokines ◽  
Redox Balance ◽  
Human Gingival Fibroblasts ◽  
Oral Disease ◽  
Oxygen Species ◽  
Gingival Fibroblasts ◽  
Peroxiredoxin 6 ◽  
Mechanistic Insight

Abstract Purpose: Periodontitis is a progressive and inflammatory oral disease and results in the damage of the supporting tissues of teeth. Peroxiredoxin 6 (PRDX6) is an antioxidant enzyme identified as a redox balance regulator. This study aimed to investigate whether PRDX6 could protect human gingival fibroblasts (HGFs) from lipopolysaccharide (LPS) induced inflammation and its mechanisms.Methods: Both inflamed and non-inflamed human gingival tissues were collected to assess the expression of PRDX6 and NRF2 by Immunohistochemistry and Western blotting. Furthermore, HGFs were stimulated with LPS, MJ33 (PRDX6 phospholipase A2 inhibitor), or ML385 (NRF2 inhibitor). The expression levels of inflammatory cytokines were measured by RT-qPCR and ELISA, and reactive oxygen species (ROS) were detected using DCFH-DA.Results: PRDX6 was downregulated in inflamed gingival tissues. In HGFs, LPS induced inflammatory cytokines and ROS was upregulated in PRDX6 knockdown cells. Furthermore, co-treatment with MJ33 alleviated LPS-induced inflammatory cytokines and ROS while inhibiting NRF2 upregulated those in HGFs.Conclusion: Therefore, this study provided a new mechanistic insight that PRDX6, regulated by the NRF2 signaling, alleviates LPS-induced periodontitis in human gingival fibroblasts.

scholarly journals Reactivity of Several Reactive Oxygen Species (ROS) with the Sesquiterpene Cacalol

2008 ◽  
Vol 3 (4) ◽  
pp. 1934578X0800300
Author(s):  
Manuel Jiménez-Estrada ◽  
Ricardo Reyes-Chilpa ◽  
Arturo Navarro-Ocaña ◽  
Daniel Arrieta-Báez
Keyword(s):  
Reactive Oxygen Species ◽  
Hydroxyl Radical ◽  
Singlet Oxygen ◽  
Furan Ring ◽  
Reactive Oxygen ◽  
Antioxidant Effect ◽  
Oxygen Species ◽  
Site Reaction ◽  
Hexanedioic Acid ◽  
Antioxidant Effects

To analyze the antioxidant effects of cacalol we determined its reactivity with different reactive oxygen species (ROS). Cacalol gave rise to cacalone by a specific site reaction with a hydroxyl radical. Singlet oxygen reacted only with the double bond of the furan ring, causing its rupture. On the other hand, ozone reacted with all double bonds in cacalol affording 2-methyl-hexanedioic acid as an end product. No reaction was observed with either superoxide or hydrogen peroxide. The potential antioxidant effect of cacalol as a scavenger of hydroxyl radical and singlet oxygen could be related to its function in the plant roots.

scholarly journals PRDX6 Alleviates Lipopolysaccharide-Induced Inflammation in Human Gingival Fibroblasts by Regulation of NRF2

2021 ◽  
Author(s):  
Wen-ying Yang ◽  
Xiang Meng ◽  
Yue-rong Wang ◽  
Qing-qing Wang ◽  
Xin He ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Phospholipase A2 ◽  
Antioxidant Enzyme ◽  
Inflammatory Cytokines ◽  
Redox Balance ◽  
Human Gingival Fibroblasts ◽  
Oral Disease ◽  
Oxygen Species ◽  
Gingival Fibroblasts ◽  
Mechanistic Insight

Periodontitis is a progressive and inflammatory oral disease and results in the damage of the supporting tissues of teeth. Peroxiredoxin6 (PRDX6) is an antioxidant enzyme and has been identified as a regulator in redox balance. This study aimed to investigate whether PRDX6 could protect human gingival fibroblasts (HGFs) from lipopolysaccharide (LPS) induced inflammation and its mechanisms. Here, both inflamed and non-inflamed human gingival tissues were collected to assess the expression of PRDX6 and NRF2 by Immunohistochemistry and Western blotting. Furthermore, HGFs were stimulated with LPS, MJ33 (PRDX6 phospholipase A2 inhibitor), or ML385 (NRF2 inhibitor). The expression levels of inflammatory cytokines were measured by RT-qPCR and ELISA, and reactive oxygen species (ROS) was detected using DCFH-DA. PRDX6 was downregulated in inflamed gingival tissues. In HGFs, LPS induced inflammatory cytokines and ROS was upregulated in PRDX6 knockdown cells. Furthermore, co-treatment with MJ33 alleviated LPS-induced inflammatory cytokines and ROS, while inhibiting NRF2 upregulated those in HGFs. Therefore, this study provided a new mechanistic insight that PRDX6, regulated by the NRF2 signaling, alleviates LPS- induced periodontitis in human gingival fibroblasts.

Flow Injection Analysis for Monitoring Antioxidant Effects on Luminol Chemiluminescence of Reactive Oxygen Species

2003 ◽  
Vol 36 (4) ◽  
pp. 749-765 ◽  
Author(s):  
Meltem Sarıahmetoğlu ◽  
R. Alan Wheatley ◽  
İclal Çakıcı ◽  
İlker Kanzık ◽  
Alan Townshend
Keyword(s):  
Reactive Oxygen Species ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Luminol Chemiluminescence ◽  
Antioxidant Effects

Antioxidant Properties of Galantamine Hydrobromide

2003 ◽  
Vol 58 (5-6) ◽  
pp. 361-365 ◽  
Author(s):  
M. Traykova ◽  
T. Traykov ◽  
V. Hadjimitova ◽  
N. Bojadgieva
Keyword(s):  
Reactive Oxygen Species ◽  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Quaternary Nitrogen ◽  
Scavenging Effect ◽  
Brain Degeneration ◽  
Antioxidant Effects

Abstract The antioxidant properties of galantamine hydrobromide ((4α,6β)-4a,5,9,10,11,12-hexahydro- 3-methoxy-11-methyl-6H-benzofuro[3a,3,2-ef][2]benzazepin-6-ol hydrobromide) were studied in vitro, using luminol-dependent chemiluminescence and spectrophotometry. It was found that this compound was a scavenger of reactive oxygen species (ROS). By comparing the antioxidant effects of galantamine ((4α,6β)-4a,5,9,10,11,12-hexahydro-3-methoxy-11-methyl- 6H-benzofuro[3a,3,2-ef][2]benzazepin-6-ol), galantamine hydrobromide, narwedine (4a, 5,9,10,11,12-hexahydro-3-methoxy-11-methyl-6H-benzofuro[3a,3,2-ef][2]benzazepin-6-one), and narwedine hydrobromide it was found that the antioxidant activity depended on the enolic OH group in the molecule. The presence of a quaternary nitrogen in the compound increased the strength of the scavenging effect. It is proposed that the antioxidant properties observed in vitro may contribute to the therapeutical effect of galantamine hydrobromide on patients with brain degeneration.

Sign in / Sign up

Export Citation Format

Share Document