STUDY OF THE INFLUENCE OF BACTERIAL LYSATE ON INDICATORS OF LYSOSOMAL PHOSPHATASE ACTIVITY IN EXPERIMENTAL PERIODONTITIS IN RATS

Introduction. One of the stages of the pathophysiological study of the parameters of mineral metabolism of alkaline (ALP) and acidic (AP) phosphatases – enzymes of bone tissue of osteoblast and osteoblast membranes in experimental periodontitis against the background of the use of bacterial lysate Respibron® was carried out. Materials and methods. Experimental periodontitis in rats was in the modification of O.I. Sukmansky. There were rats with experimental periodontitis and rats treated by Respibron (10 mg/kg 1 time per day) or Imudon for 30 days. Determination of alkaline (ALP) and acidic (AP) phosphatases activity (pH 4.8 and 10.5) was performed according to the methodological recommendations of AP Levitsky et al. Discussion of results. It was found that by the end of the 90-day period of the experiment, a statistically stable course of chronic damage to the periodontium was observed in terms of ALP and AP, which exceeded the intact ones by 1.93 and 1.4 times, respectively, which reflects the hypoergic state of chronic periodontal inflammation. A powerful corrective effect of the drugs Respibron® and Imudon® on markers of osteoblast and osteoblast activity in experimental periodontitis has been proven. Established a stimulating effect on osteoregeneration Respibron® in the hypoergic course of the inflammatory process. The therapeutic effect of Respibron® is more expressive and powerful compared to Imudon®. Conclusions.The results obtained make it possible to continue the study of the course of experimental periodontitis in order to establish the mechanisms of development, protection, and restoration of tissues under the conditions of the pharmacological correction Respibron®.

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STUDY OF THE INFLUENCE OF BACTERIAL LYSATE ON INDICATORS OF LYSOSOMAL PHOSPHATASE ACTIVITY IN EXPERIMENTAL PERIODONTITIS IN RATS

Likarska sprava ◽

10.31640/jvd.1-2.2021(8) ◽

2021 ◽

pp. 51-56

Author(s):

M. S. Evtushenko ◽

О. Yu. Koshоva ◽

S. I. Kryzhna ◽

T. I. Tyupka

Keyword(s):

Phosphatase Activity ◽

Inflammatory Process ◽

Mineral Metabolism ◽

Bacterial Lysate ◽

Corrective Effect ◽

Periodontal Inflammation ◽

Osteoblast Activity ◽

Experimental Periodontitis ◽

Mechanisms Of Development

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STUDY OF THE INFLUENCE OF BACTERIAL LYSATE ON OXIDATIVE STRESS INDICATORS IN EXPERIMENTAL PERIODONTIS IN RATS

Likarska sprava ◽

10.31640/jvd.7-8.2020(6) ◽

2020 ◽

pp. 50-55

Author(s):

M. S. Ievtushenko ◽

A. Yu. Koshоva ◽

S. I. Kryzhna ◽

T. I. Tyupka

Keyword(s):

Oxidative Stress ◽

Point Of View ◽

Bacterial Lysate ◽

Compensatory Mechanisms ◽

Stress Indicators ◽

Experimental Periodontitis ◽

Pharmacological Correction ◽

Mechanisms Of Development ◽

High Degree ◽

Oxidative Stress Indicators

One of the stages of the pathophysiological study of the prooxidant-antioxidant state in terms of oxidative stress of the oral mucosa in rats with experimental periodontitis and pharmacological correction with bacterial lysate was carried out. Reproduction of periodontitis reliably led to a high degree of impairment of the lipid peroxidation (LPO) and antioxidant system (AOS), the red/ox-balance coefficient. It was proved that by the end of the 90-day period of observation of the course of periodontitis, the power of natural compensatory mechanisms acquired a state of decompensation, which was manifested by a high red/ox-balance ratio, inhibition of the functional activity of both non-enzymatic and enzymatic AOS links in the homogenate of gum tissue of control animal pathology: K (LPO/AOS) = 0.39, in intact animals = 1. The use of Respibron for 30 days contributed to the normalization of the antioxidant-prooxidant index, equal to 0.9 in this group and was statistically significantly higher than that in the Imudon group (0.64). The results obtained make it possible to continue studies of the course of experimental periodontitis from the point of view of establishing the mechanisms of development, protection, and restoration of tissues under conditions of pharmacological correction with the bacterial lysate Respibron®.

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Study of influence of bacterial lysate on cytokine indicators in experimental periodontis in rats

EUREKA Health Sciences ◽

10.21303/2504-5679.2021.001970 ◽

2021 ◽

pp. 73-80

Author(s):

Mariia Ievtushenko ◽

Elena Kosheva ◽

Svitlana Kryzhna

Keyword(s):

Immune Response ◽

Point Of View ◽

C Reactive Protein ◽

Bacterial Lysate ◽

Reactive Protein ◽

Immunological Markers ◽

Experimental Periodontitis ◽

Anti Inflammatory ◽

Bacterial Lysates ◽

Mechanisms Of Development

The aim of the research: to optimize the treatment of patients with a disturbed cytokine profile by a bacterial lysate in an experimental model of periodontitis against the background of changes in the local and systemic immune response. Materials and methods: prospective study has been conducted on experimental periodontitis in 42 rats for 90 days. The animals were treated by solution of «Respibron» and the reference drug «Imudon». The concentration of cytokines in the blood serum was determined by the enzyme immunoassay, circulating immune complexes – precipitation method is measured on a spectrophotometer, C-reactive protein – using a latex diagnosticum. All interventions and euthanasia of animals were carried out in compliance with the European principles. Results: the obtained data from the study indicate a high degree a state of decompensation of experimental periodontitis. An immuno-inflammatory reaction preceding periodontitis is accompanied by the disturbances of the cytokine balance, local and systemic immune response. Applying of bacterial lysates led to the compensation of bacterial dysbiosis, restoration of the balance of the activity of pro- and anti-inflammatory cytokines, and a decrease the hyperactivity of immunological markers. Conclusion: the studies carried out have shown that the use of bacterial lysate is a pathogenetically substantiated component of therapeutic tactics. The normalizing effect of the lysate on the parameters of the immune response and cytokine profile has been reliably shown. Considering the immuno-inflammatory reaction at the experimental periodontitis, accompanied by the cytokine imbalance, study the pharmacological correction by bacterial lysate should continue from the point of view of establishing the mechanisms of development, protection, and restoration of tissues. It has been proved that by the end of the 90-day period of observation of the course of experimental periodontitis, the power of natural compensatory mechanisms acquired a state of decompensation. It has manifested by increased pro-inflammatory cytokine IL-1β in 1.7 times, and TNF-α in 3 times, decreased anti-inflammatory cytokine IL-10 in 1.6 times; increased the concentration of CIC in the blood in 2 times, C-reactive protein in 4.4 times at the local level in the gum tissue homogenate of the control pathology group of animals. The powerful corrective effect of bacterial lysates «Respibron» and «Imudon» on the indicators of the cytokine series on the model of chronic experimental periodontitis has been reliably established. The normalizing effect is due to the compensation of bacterial dysbiosis, restoration of the balance of the activity of pro- and anti-inflammatory cytokines, and a decrease in the hyperactivity of immunological markers. The therapeutic effect of «Respibron» (bacterial lysate of 13 strains of microorganisms) can be assessed as expressive and more powerful in comparison with «Imudon». The obtained results make it possible to continue the study of the course of experimental periodontitis from the point of view of establishing the mechanisms of development, protection, and restoration of tissues under conditions of pharmacological correction with bacterial lysate «Respibron».

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Histological study of the effect of bacterial lysates on the state of periodontal tissue in experimental periodontitis in rats

ScienceRise Medical Science ◽

10.15587/2519-4798.2021.241983 ◽

2021 ◽

pp. 41-47

Author(s):

Mariia Ievtushenko ◽

Olena Koshova ◽

Svitlana Kryzhna

Keyword(s):

Histological Study ◽

Local Level ◽

The Body ◽

Control Group ◽

Central Research ◽

Bacterial Lysate ◽

Reference Drug ◽

Corrective Effect ◽

Experimental Periodontitis ◽

Bacterial Lysates

The aim of the research: to experimentally study at the histological and morphological level the degree of the corrective effect of bacterial lysate of the disturbed non-specific defense of the body on the model of periodontitis based on the Central Research Laboratory of the National University of Pharmacy. Materials and methods: prospective study has been conducted on experimental periodontitis in 42 rats for 90 days. The animals were treated with «Respibron» and the reference drug «Imudon». Histological and morphometric studies were carried out according to standard methods. Micropreparations were viewed under a Granum DCM 310 digital video camera. All interventions and euthanasia of animals were carried out in compliance with the European principles. Results: by the end of 90 days of experimental periodontitis at the local level in the homogenate of animal gum tissue compensatory mechanisms are depleted and differed from the norm by 397 times. The dynamics of the studied morphometric and histological parameters of "Respibron" was similar to the "Imudon", but the magnitude of destruction was less pronounced and differed at the end of the experiment by 17.2 times in comparison with the intact control, and in the control group the results improved by 23.1 times. Conclusion: the obtained data from the study indicate a high decompensation of experimental periodontitis. It is characterized by the formation of periodontal pockets and inflammatory bone loss. The magnitude of destruction differed from the norm by 397 times. Applying of bacterial lysates led to the compensation of bacterial dysbiosis, restoration of the tissues of paradont. The therapeutic effect of "Respibron" can be assessed as more powerful in comparison with "Imudon" in terms of the studied morphometric and histological parameters: the magnitude of improvement "Respibron" was 3.72 times higher than the indicators of "Imudon". We should continue the study of experimental periodontitis as mechanisms of development, protection, and restoration of tissues under conditions of pharmacological correction by bacterial lysate "Respibron"

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Determination of clinical efficiency of deproteinized dialysate from blood of milk-fed calves after endoprosthetics in patients with ventral hernias

I P Pavlov Russian Medical Biological Herald ◽

10.23888/pavlovj2020283323-333 ◽

2020 ◽

Vol 28 (3) ◽

pp. 323-333

Author(s):

Sergej V. Ivanov ◽

Ilya S. Ivanov ◽

Evgenij G. Obyedkov ◽

Liliya P. Popova

Keyword(s):

Abdominal Wall ◽

Inflammatory Reaction ◽

Inflammatory Process ◽

Anterior Abdominal Wall ◽

Dynamic Change ◽

Main Group ◽

Control Group ◽

Surgical Department ◽

Regeneration Stage

Aim. To study the influence of deproteinized dialysate from blood of milk-fed calves on the type of exudative discharge and dynamics of inflammatory reaction after hernia repair with plastics of the anterior abdominal wall with hernioendoprosthesis of polypropylene. Materials and Methods. The study involved 59 patients being on stationary treatment in the surgical department of Kursk Regional Clinical Hospital. Patients were hospitalized for the herniation of small or medium dimension. The patients were divided to two groups: the main (n=30) and control (n=29) groups. After endoprosthetics, the patients of the control group were given complex conservative treatment. The patients of the main group, besides standard treatment, were administered deproteinized dialysate from blood of milk-fed calves intravenously in drips 10 ml + 200 ml of 0.9% sodium chloride solution within 7 days. For cytological examination and determination of the type of cytograms, the traumatic discharge was collected and analyzed using the method of M.F. Kamaev and M.A. Palthsev. Results. Cytomorphometric examination was conducted on the third, fifth and seventh day after endoprosthetics with the aim of studying dynamic changes. Determination of cell composition and also of its changes characteristic of each studied period, was necessary for obtaining further information characterizing inflammatory process in the region of placement of the endoprosthesis. After endoprosthetics in patients who were administered hemodialysate, the inflammatory reaction was less pronounced than in patients who did not receive the preparation. This was associated with a more dynamic change of stages of the inflammatory process. In patients of the main group who received deproteinized dialysate, regenerative type of inflammation first appeared on the fifth day and made 6.9%; by the seventh day the share of patients with the regenerative type rose to 17.5%, while in the patients of the control group no regeneration stage was observed in both periods. Conclusion. Analysis of the efficiency of influence of hemodialysate on the inflammatory reaction in plastics of the anterior abdominal wall with polypropylene endoprosthesis evidences faster course of all stages of inflammation and reduction of its intensity by 10%.

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