scholarly journals Histological study of the effect of bacterial lysates on the state of periodontal tissue in experimental periodontitis in rats

2021 ◽  
pp. 41-47
Author(s):  
Mariia Ievtushenko ◽  
Olena Koshova ◽  
Svitlana Kryzhna
Keyword(s):  
Histological Study ◽  
Local Level ◽  
The Body ◽  
Control Group ◽  
Central Research ◽  
Bacterial Lysate ◽  
Reference Drug ◽  
Corrective Effect ◽  
Experimental Periodontitis ◽  
Bacterial Lysates

The aim of the research: to experimentally study at the histological and morphological level the degree of the corrective effect of bacterial lysate of the disturbed non-specific defense of the body on the model of periodontitis based on the Central Research Laboratory of the National University of Pharmacy. Materials and methods: prospective study has been conducted on experimental periodontitis in 42 rats for 90 days. The animals were treated with «Respibron» and the reference drug «Imudon». Histological and morphometric studies were carried out according to standard methods. Micropreparations were viewed under a Granum DCM 310 digital video camera. All interventions and euthanasia of animals were carried out in compliance with the European principles. Results: by the end of 90 days of experimental periodontitis at the local level in the homogenate of animal gum tissue compensatory mechanisms are depleted and differed from the norm by 397 times. The dynamics of the studied morphometric and histological parameters of "Respibron" was similar to the "Imudon", but the magnitude of destruction was less pronounced and differed at the end of the experiment by 17.2 times in comparison with the intact control, and in the control group the results improved by 23.1 times. Conclusion: the obtained data from the study indicate a high decompensation of experimental periodontitis. It is characterized by the formation of periodontal pockets and inflammatory bone loss. The magnitude of destruction differed from the norm by 397 times. Applying of bacterial lysates led to the compensation of bacterial dysbiosis, restoration of the tissues of paradont. The therapeutic effect of "Respibron" can be assessed as more powerful in comparison with "Imudon" in terms of the studied morphometric and histological parameters: the magnitude of improvement "Respibron" was 3.72 times higher than the indicators of "Imudon". We should continue the study of experimental periodontitis as mechanisms of development, protection, and restoration of tissues under conditions of pharmacological correction by bacterial lysate "Respibron"

scholarly journals Prevention of subinvolution of the uterus of cows using acidum succinicum

2021 ◽  
pp. 49-52
Author(s):  
Larysa Fedonyuk ◽  
Yaroslav Stravskyy ◽  
Vira Khavtur ◽  
Roman Sachuk
Keyword(s):  
Animal Experiments ◽  
Reproductive Function ◽  
The Body ◽  
Control Group ◽  
Central Research ◽  
National Medical ◽  
Uterine Involution ◽  
Service Period ◽  
Activity Of Enzymes ◽  
Medical University

Aim. To establish the effect of acidum succinicum on the activity of enzymes in the blood of cows after calving and the course of uterine involution. Materials and Methods. The study of enzyme activity was carried out in the Central Research Laboratory of I. Horbachevsky Ternopil National Medical University (Certificate of technical competence № 001/18, issued on September 26, 2018, valid until September 25, 2023). Results of the research. After the use of acidum succinicum in the body of cows of the experimental group, the activity of alanine aminotransferase increased by 12,0 % (p≤0,05), and the activity of aspartate aminotransferase, on the contrary, decreased by 30,0 % (p≤0,001), which indicates a positive effect on hepatocytes. Confirmation of this is the Ritis factor, which increased 1,4 times (p≤0,05). All animal experiments were conducted according to with the recommendations of ARRIVE and the British Animal Act 1986 and Directive 2010/63 / EU on the protection of animals for scientific investigation. In the body of cows of the control group, the activity of alanine and aspartateaminotransferases increased, respectively, by 14,0 % (p≤0,05) and 47,0 % (p≤0,001) against a background of a 1,2-fold decrease (p≤0,05) Ritis factor. A decrease in bilirubin content of 56,0 % (p≤0,001) in the body of cows after the use of acidum succinicum and an increase in its content by 2,2 times (p≤0,001) in the body of control cows indicates liver disease, which is accompanied by affection of its parenchyma, which may be a consequence of intoxication of their body in the last trimester of pregnancy. Conclusion. Acidum succinicum, applied to cows after calving, prevents the development of inflammation of the reproductive system, helps to restore reproductive function, reduces the duration of the service period to 80,0±2,0 days, and the insemination index to 1,3

scholarly journals Cafeteria Diet-Induced Metabolic and Cardiovascular Changes in Rats: The Role of Piper nigrum Leaf Extract

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Dorothee L. E. Mballa ◽  
Fanta S. A. Yadang ◽  
Armelle D. Tchamgoue ◽  
Jean R. Mba ◽  
Lauve R. Y. Tchokouaha ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Weight Gain ◽  
Relative Weight ◽  
The Body ◽  
Cafeteria Diet ◽  
Male Rats ◽  
Piper Nigrum ◽  
Control Group ◽  
Reference Drug ◽  
Cardiovascular Changes

Background. Cafeteria diet is known to induce excessive body fat accumulation (obesity) that could cause metabolic and cardiovascular changes and even death. The increase in prevalence over time and the failure in treatment options make obesity a real public health problem. The present study assessed the preventive effect of the hydro-ethanolic extract of the Piper nigrum leaf on the development of metabolic and cardiovascular changes in cafeteria diet fed Wistar rats. Methods. Thirty-six male rats were divided into 5 groups of 6 rats each: a normal control group (Nor.), a negative control group (Neg.), two groups administered different doses of extract in mg/kg (E250 and E500), and a group administered atorvastatin 10 mg/kg (Ator., reference drug). The animals were fed with experimental diets (standard and cafeteria) for a period of 5 weeks. Food and water intake were assessed daily, and the body weight assessed weekly. At the end of the feeding, plasma lipid profile and markers of hepatic and renal function were assessed. Furthermore, the relative weights of the adipose tissue and the organs were assessed. The liver, kidneys, and heart homogenates were assessed for markers of oxidative stress while the aorta was histopathologically examined. Results. Cafeteria diet-induced weight gain of 30% and increased triglyceride, total cholesterol, and low-density lipoprotein cholesterol level of more than 50%. Equally, an increase in the relative weight of accumulated adipose tissues of more than 90%, oxidative stress, and alteration in the organ structure were visible in cafeteria diet fed rats (Neg). Treatment with P. nigrum extract significantly prevented weight gain, dyslipidemia, oxidative stress, and alteration in the architecture of the aorta. The effect of P. nigrum extract was comparable to that of the reference drug. Conclusion. Piper nigrum leaf may prevent weight gain and possess cardioprotective activity with a strong antioxidant activity.

scholarly journals Effect of Diabetes on the Male Reproductive System—A Histomorphological Study

2019 ◽  
Vol 36 (01) ◽  
pp. 017-023 ◽  
Author(s):  
Sushma Kotian ◽  
Ashok Kumar ◽  
Sanchari Mallik ◽  
Nandini Bhat ◽  
Anne Souza ◽  
...  
Keyword(s):  
Reproductive System ◽  
Histological Study ◽  
Autoimmune Disorder ◽  
Diabetic Rats ◽  
The Body ◽  
Male Reproductive System ◽  
Diabetic Group ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Minimum Diameter

Introduction Type 1 diabetes is an autoimmune disorder characterized by lack of insulin production by the β cells of the pancreas. This lack of insulin causes a variety of systemic effects on the metabolism of the body, one of which is reproductive dysfunction. The present study investigates the effects of diabetes on the male reproductive system of streptozotocin (STZ)-induced diabetic rats. Material and Methods A total of 18 adult male Wistar rats weighing ∼ between 250 and 300 g were included in the present study. The animals were divided into normal and diabetic groups. The diabetic group was further subdivided into 2 subgroups with durations of 24 and 48 days. A single dose of STZ (40 mg/kg body weight) was administrated intraperitoneally to the animals of the diabetic group. After the planned duration, the testes and epididymides were dissected, and their gross weight was measured. The tissues were then processed for histological study. Results The gross weight of the testes and epididymides in diabetic rats at 24 and 48 days showed a decrease in comparison to the control. (p < 0.01 for testes and epididymides).Diabetic animals presented a significant decrease in the diameter of the seminiferous tubules compared with the control group (p < 0.01). The epididymides in the diabetic groups showed a considerable reduction in the tubular surface area compared with the control group (p < 0.01). There was also a reduction in the mean diameter, which was measured using the maximum and minimum diameter of the tubules (p < 0.01). Conclusion The present study is an insight into the adverse effects that diabetes can have on the tissue structure of the testes, of the epididymides, and ultimately on the process of spermatogenesis.

scholarly journals AMELIORATIVE EFFECTS OF MELATONIN AGAINST 2,4-DICHLOROPHENOXYACETIC ACID TOXICITY IN KIDNEY OF MICE – A HISTOLOGICAL STUDY.

2018 ◽  
Vol 11 (1) ◽  
pp. 78 ◽  
Author(s):  
Ashlesh M Upadhyaya ◽  
Mandava V Rao ◽  
Devendrasinh D Jhala
Keyword(s):  
Body Weight ◽  
Histological Study ◽  
Group Iv ◽  
The Body ◽  
Control Group ◽  
Dichlorophenoxyacetic Acid ◽  
Group Iii ◽  
Group I ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Acid Toxicity

 Objective: The compound, 2,4-dichlorophenoxyacetic acid (2,4-D), is a systemic phenoxy herbicide and a plant growth regulator. Histopathological effects of 2,4-D alone and in combination with melatonin on the kidney in adult albino male mice of the Swiss strain were evaluated in the present study.Methods: Twenty adult male albino mice weighing between 30 and 40 g were assigned into four groups with five mice in each group. Group I served as control; Group II - melatonin alone (10 mg/kg body weight); Group III - 2,4-D alone (50 mg/kg body weight); and Group IV - 2,4-D + melatonin was given orally for 45 days.Results: It revealed a significant reduction in the body and kidney weights (p<0.001 and p<0.01 respectively) of mice treated with 2,4-D, while the body and kidney weights after treatment of melatonin and 2,4-D + melatonin were comparable to control. Histological study revealed that 2,4-D induced nephrotoxicity evidenced by various histological changes including shrinkage of the glomerulus, tubular dilation, hypertrophy of tubular epithelium, and degeneration of renal tubules.Conclusion: Treating animals with the melatonin along with 2,4-D showed mitigative effects of melatonin and minimized the structural alteration in the kidney of mice appeared due to 2,4-D toxicity. 

scholarly journals STUDY OF THE INFLUENCE OF BACTERIAL LYSATE ON INDICATORS OF LYSOSOMAL PHOSPHATASE ACTIVITY IN EXPERIMENTAL PERIODONTITIS IN RATS

2021 ◽  
pp. 51-56
Author(s):  
M. S. Evtushenko ◽  
О. Yu. Koshоva ◽  
S. I. Kryzhna ◽  
T. I. Tyupka
Keyword(s):  
Phosphatase Activity ◽  
Inflammatory Process ◽  
Mineral Metabolism ◽  
Bacterial Lysate ◽  
Corrective Effect ◽  
Periodontal Inflammation ◽  
Osteoblast Activity ◽  
Experimental Periodontitis ◽  
Mechanisms Of Development

Introduction. One of the stages of the pathophysiological study of the parameters of mineral metabolism of alkaline (ALP) and acidic (AP) phosphatases – enzymes of bone tissue of osteoblast and osteoblast membranes in experimental periodontitis against the background of the use of bacterial lysate Respibron® was carried out. Materials and methods. Experimental periodontitis in rats was in the modification of O.I. Sukmansky. There were rats with experimental periodontitis and rats treated by Respibron (10 mg/kg 1 time per day) or Imudon for 30 days. Determination of alkaline (ALP) and acidic (AP) phosphatases activity (pH 4.8 and 10.5) was performed according to the methodological recommendations of AP Levitsky et al. Discussion of results. It was found that by the end of the 90-day period of the experiment, a statistically stable course of chronic damage to the periodontium was observed in terms of ALP and AP, which exceeded the intact ones by 1.93 and 1.4 times, respectively, which reflects the hypoergic state of chronic periodontal inflammation. A powerful corrective effect of the drugs Respibron® and Imudon® on markers of osteoblast and osteoblast activity in experimental periodontitis has been proven. Established a stimulating effect on osteoregeneration Respibron® in the hypoergic course of the inflammatory process. The therapeutic effect of Respibron® is more expressive and powerful compared to Imudon®. Conclusions.The results obtained make it possible to continue the study of the course of experimental periodontitis in order to establish the mechanisms of development, protection, and restoration of tissues under the conditions of the pharmacological correction Respibron®.

scholarly journals Study of influence of bacterial lysate on cytokine indicators in experimental periodontis in rats

2021 ◽  
pp. 73-80
Author(s):  
Mariia Ievtushenko ◽  
Elena Kosheva ◽  
Svitlana Kryzhna
Keyword(s):  
Immune Response ◽  
Point Of View ◽  
C Reactive Protein ◽  
Bacterial Lysate ◽  
Reactive Protein ◽  
Immunological Markers ◽  
Experimental Periodontitis ◽  
Anti Inflammatory ◽  
Bacterial Lysates ◽  
Mechanisms Of Development

The aim of the research: to optimize the treatment of patients with a disturbed cytokine profile by a bacterial lysate in an experimental model of periodontitis against the background of changes in the local and systemic immune response. Materials and methods: prospective study has been conducted on experimental periodontitis in 42 rats for 90 days. The animals were treated by solution of «Respibron» and the reference drug «Imudon». The concentration of cytokines in the blood serum was determined by the enzyme immunoassay, circulating immune complexes – precipitation method is measured on a spectrophotometer, C-reactive protein – using a latex diagnosticum. All interventions and euthanasia of animals were carried out in compliance with the European principles. Results: the obtained data from the study indicate a high degree a state of decompensation of experimental periodontitis. An immuno-inflammatory reaction preceding periodontitis is accompanied by the disturbances of the cytokine balance, local and systemic immune response. Applying of bacterial lysates led to the compensation of bacterial dysbiosis, restoration of the balance of the activity of pro- and anti-inflammatory cytokines, and a decrease the hyperactivity of immunological markers. Conclusion: the studies carried out have shown that the use of bacterial lysate is a pathogenetically substantiated component of therapeutic tactics. The normalizing effect of the lysate on the parameters of the immune response and cytokine profile has been reliably shown. Considering the immuno-inflammatory reaction at the experimental periodontitis, accompanied by the cytokine imbalance, study the pharmacological correction by bacterial lysate should continue from the point of view of establishing the mechanisms of development, protection, and restoration of tissues. It has been proved that by the end of the 90-day period of observation of the course of experimental periodontitis, the power of natural compensatory mechanisms acquired a state of decompensation. It has manifested by increased pro-inflammatory cytokine IL-1β in 1.7 times, and TNF-α in 3 times, decreased anti-inflammatory cytokine IL-10 in 1.6 times; increased the concentration of CIC in the blood in 2 times, C-reactive protein in 4.4 times at the local level in the gum tissue homogenate of the control pathology group of animals. The powerful corrective effect of bacterial lysates «Respibron» and «Imudon» on the indicators of the cytokine series on the model of chronic experimental periodontitis has been reliably established. The normalizing effect is due to the compensation of bacterial dysbiosis, restoration of the balance of the activity of pro- and anti-inflammatory cytokines, and a decrease in the hyperactivity of immunological markers. The therapeutic effect of «Respibron» (bacterial lysate of 13 strains of microorganisms) can be assessed as expressive and more powerful in comparison with «Imudon». The obtained results make it possible to continue the study of the course of experimental periodontitis from the point of view of establishing the mechanisms of development, protection, and restoration of tissues under conditions of pharmacological correction with bacterial lysate «Respibron».

scholarly journals ANTIDIABETIC POTENTIAL OF PERILLYL ALCOHOL, A MONOTERPENE ON HIGH-FAT DIET LOW-DOSE STREPTOZOTOCIN-INDUCED DIABETES IN EXPERIMENTAL RATS

2019 ◽  
pp. 230-235
Author(s):  
TOWSEEF HASSAN ◽  
ELANCHEZHIYAN C ◽  
INSHA NASEER
Keyword(s):  
Blood Glucose ◽  
High Fat Diet ◽  
Low Dose ◽  
Histological Study ◽  
Perillyl Alcohol ◽  
Diabetic Rats ◽  
Control Group ◽  
High Fat ◽  
Plasma Parameters ◽  
Reference Drug

Objective: The aim of the study was to evaluate the antidiabetic potential of Perillyl alcohol (POH) in high-fat diet (HFD) and low-dose streptozotocin (STZ)-induced diabetic Wistar rats. Methods: In experimental rats fed with HFD for 4 weeks, diabetes was induced by single intraperitoneal injection of STZ (35 mg/kg body weight [BW]). Three days after STZ induction, the hyperglycemic rats were treated with POH orally at the doses of 50 and 100 mg/kg BW daily for 30 days. Glibenclamide (6 mg/kg BW) was used as reference drug. Blood glucose level, BW, fluid intake, and food intake such as parameters were analyzed throughout the experiment period. Serum and plasma biochemical parameters were also estimated. Assay on insulin resistance was also done. Histological study of the pancreas and liver was also performed. Results: POH orally at the doses of 50 and 100 mg/kg BW for 30 days significantly (p<0.05) and dose-dependently reduced and normalized blood glucose levels as compared to that of HFD+STZ control group. Serum and plasma parameters were significantly (p<0.05) restored toward the normal levels in POH-treated rats as compared to HFD-STZ control animals. Histological study of liver and pancreas also shows the prominent regeneration in POH and glibenclamide treated rats. Conclusion: The present study concludes that POH demonstrated promising antidiabetic action in HFD STZ-induced diabetic rats.

scholarly journals Hepatoprotective potentials of Picralima nitida against in vivo carbon tetrachloride-mediated hepatotoxicity

2016 ◽  
Vol 5 (1) ◽  
pp. 6-9
Author(s):  
Idu MacDonald ◽  
◽  
Ovuakporie-Uvo Oghale ◽  
Eze Gerald Ikechi ◽  
Okoro Amarachi Orji ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Negative Control ◽  
Seed Extract ◽  
The Body ◽  
Control Group ◽  
Reference Drug ◽  
Antioxidant Agents

This research aimed at investigating the in vivo Carbon tetrachloride (CCl4)-mediated hepatotoxicity of methanolic seed extract of Picralima nitida (P. nitida) using Wistar rats. Twenty five (25) rats randomly selected into five groups of five animals were used in this research. Group 1 was administered Normal saline (Negative control); Group II was administered 1 ml of Carbon tetrachloride only (Positive control/ Reference drug); Group III, IV and V got 10 ml P. nitida extract + 1ml Carbon tetrachloride; 100 ml P. nitida extract + 1ml Carbon tetrachloride and 1000 ml P. nitida extract + 1ml Carbon tetrachloride respectively. Results show that treatment with P. nitida extract had no adverse effect on the body weight of Wistar rats. Biochemical analysis show increase in CAT and GSH which are good antioxidant agents. Photomicrographs show moderate amelioration from steatosis caused by Carbon tetrachloride in the treatment groups. Further study is recommended to verify if P. nitida seed extract can completely ameliorate and possibly reverse fat degeneration of liver cells induced by Carbon tetrachloride.

scholarly journals Стан системи нітроген (ІІ) оксиду в щурів з пародонтитом на фоні гіпер- та гіпотиреозу

2018 ◽  
Author(s):  
V. V. Shcherba ◽  
M. M. Korda
Keyword(s):  
Periodontal Disease ◽  
No Synthase ◽  
The Body ◽  
Male Rats ◽  
Tissue Homogenate ◽  
Control Group ◽  
Periodontal Tissue ◽  
Social Significance ◽  
Periodontal Tissues ◽  
Experimental Periodontitis

Introduction. Inflammatory periodontal disease is one of the most urgent problems of dentistry, which has a social significance due to the high prevalence, pronounced changes in the tissues of the periodontal disease and the body of the patient as a whole, and the defeat of young people.The aim of the study – to investigate the functional state of the nitrogen (II) oxide system in rats with periodontitis without concomitant pathology and against the background of hyper- and hypothyroidism.Research Methods. The study was carried out on 48 white non-linear male rats. The total activity of NO-synthase (NOS) was determined colorimetrically by the number of formed nitrates and nitrites in the incubation medium. The total content of nitrates and nitrites (NOx) was determined by the Gris method.Results and Discussion. Experimental periodontitis is accompanied by increased general activity of NO-synthase in periodontal tissue homogenate by 2.2 times vs control. NOx content in the serum of animals with periodontitis increased by 46.2% and in the periodontal tissue homogenate – by 74.7% compared with the control. In rats with periodontitis against hyperthyroidism, NOS activity increased by 3.9 times relative to the control group of animals and by 75.9% exceeded the rate of rats with periodontitis without concomitant pathology. In rats with periodontitis, against the background of hypothyroidism, the activity of NOS was 29.6% higher than that of rats with periodontitis without concomitant pathology and 2.9-fold of control.Conclusions. Experimental periodontitis is accompanied by a marked increase in the intensity of nitroxidergic processes both in the homogenate of periodontal tissues and in the blood. The imbalance of thyroid hormones increases the synthesis of nitrogen (II) oxide in the experimental periodontitis, especially expressed in hyperthyroidism.

scholarly journals The efficiency of using of Alfasorb in piglets feeding

2018 ◽  
Vol 20 (88) ◽  
pp. 120-124
Author(s):  
O. P. Reshetnychenko ◽  
O. T. Piven ◽  
E. U. Rozum ◽  
V. I. Savshenko ◽  
A. I. Levinsky ◽  
...  
Keyword(s):  
Histological Study ◽  
Physiological State ◽  
Serum Levels ◽  
The Body ◽  
Daily Weight Gain ◽  
Feed Additive ◽  
Economic Damage ◽  
Control Group ◽  
Large White ◽  
Experimental Group

Mycotoxins cause severe diseases in organisms of human and animals. They are difficult to diagnose, cause great economic damage. Sorbents are able to effectively bind and remove from the body through the digestive tract with a therapeutic or prophylactic purpose various endogenous and ekzogenous substances, microorganisms and they toxins, supermolecular structures and cells. The aim of the work is to study the effectiveness of use of Alfasorb for the neutralization of mixed fodders affected by mycotoxins and they impact on blood’s indexes and productive qualities of piglets of Large White Breed. Experience was conducted on 30 ty piglets. Animals of the control group received mixed feed that was produced in the conditions of the farm. The pigs of experimental group in addition to the basic diet received Alfasorb. We determined blood’s indexes for study the effect of feed additive Alfasorb on the physiological state of animals at 4 months of age. A morphological and histological study of liver was carried out. The analysis of the average daily weight gain of piglets showed that it was higher in period of growth in the animals of experimental group. In the animals of the experimental groups there was an increase in the number of red blood cells in comparison with the control. An unreliable decrease in blood serum levels which characterize the level of intermediate metabolism of proteins in the body (content of urea, creatinine and common bilirubin) was established in the animals of the experimental group as compared to the control group respectively by 22.83% ( td = 0.98, P ≤ 0.05), 7.12% (td = 1.92, P ≤ 0.05) and by 20.54% (td = 0.86, P ≤ 0.05). Most hepatocytes had large nucleus and intense color of the cytoplasm. This indicates the absence of alterative processes in the body. The results of the studies make it possible to assert that the inclusion the 0.2% Alfasorb to the compound of feed which contaminated with mycotoxins significantly reduces their negative effect on the piglets organism, has a positive effect on the growth rate, on the course of metabolic processes, also it promotes an increase in the liver of medium-sized hepatocyte nuclei, stimulates biosynthetic processes in that cells and ensures the formation of a full structure of histohematological barriers in the organism.

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