scholarly journals MORPHOLOGICAL CHARACTERISTICS OF PERIPHERAL BLOOD OF COWS INFECTED WITH BLV AND THE FREQUENCY OF REGISTRATION OF LEUKEMOID REACTIONS IN COWS WITH LEUKEMIA-AFFECTED HERDS

2020 ◽  
pp. 66-72
Author(s):  
P. N. Smirnov ◽  
S. M. Chudum ◽  
I. V. Trostyansky ◽  
O. S. Kotlyarova
Keyword(s):  
Successful Treatment ◽  
Peripheral Blood ◽  
Serum Proteins ◽  
Morphological Characteristics ◽  
Controlled Experiments ◽  
Individual Analysis ◽  
Morphological Composition ◽  
Inflammatory Processes

In controlled experiments during planned studies of cattle for leukemia, animals that showed leukemoid changes in blood – quantitative redistribution of granulocytes and agranulocytes-were identified. Individual analysis revealed that granulocytosis was detected in animals with inflammatory processes. In addition, the article presents comparative indicators of serum proteins in cows at the hematological stage of the leukemic process, with the manifestation of leukemoid reactions and in clinically healthy cows. Characteristic changes in the synthesis of immunoglobulins in cows with leukemia and leukemoid changes in the morphological composition of blood were established. With successful treatment of inflammatory processes, the hematological status of cows is restored to the initial indicators.

Nutritive value and morphological characteristics of Mombaça grass managed with different rotational grazing strategies

2019 ◽  
Vol 157 (7-8) ◽  
pp. 592-598
Author(s):  
S. C. Da Silva ◽  
A. A. O. Bueno ◽  
R. A. Carnevalli ◽  
G. P. Silva ◽  
M. B. Chiavegato
Keyword(s):  
Nutritive Value ◽  
Morphological Characteristics ◽  
Statistical Design ◽  
Panicum Maximum ◽  
Forage Production ◽  
Light Competition ◽  
Area Index ◽  
Light Utilization ◽  
Morphological Composition ◽  
Acid Detergent Fibre

AbstractLight competition increases and plants’ growth pattern change to optimize light utilization when the leaf area index increases. It has been previously shown that using 95% canopy light interception (LI) as a grazing frequency criterion resulted in a greater proportion of leaves and a lower proportion of stem. The objective of the study was to characterize the forage production, morphological composition and nutritive value of Panicum maximum cv Mombaça. The experiment was carried out during summer, autumn–winter and spring. Treatments corresponded to combinations of two pre-grazing conditions (95% and maximum LI at pre-grazing; LI95% and LIMax, respectively) and two post-grazing heights (PGHs; 30 and 50 cm). The statistical design was a randomized complete block, with a 2 × 2 factorial arrangement. Swards managed with LI95% had greater proportions of leaves and lower proportions of stems compared to LIMax. Leaf proportion was lower during autumn–winter compared to summer and spring. The LI95% had greater crude protein (CP) and digestibility (IVOMD), and lower acid detergent fibre (ADF) concentrations than LIMax. The 50 cm PGH pastures had greater CP content and IVOMD, and lower ADF content than 30 cm PGH pastures. Lower IVOMD was observed during autumn–winter than summer and spring. The variability observed on morphological characteristics was primarily associated with seasonality, whilst the nutritive value was primarily affected by grazing management. The pre-grazing target of LI95% combined with 50 cm PGH was the combination that resulted in an increased proportion of leaves, decreased stems in basal stratum and the greatest nutritive value of the produced forage.

scholarly journals Blood proteome profiling using aptamer-based technology for rejection biomarker discovery in transplantation

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Andrey V. Shubin ◽  
Branislav Kollar ◽  
Simon T. Dillon ◽  
Bohdan Pomahac ◽  
Towia A. Libermann ◽  
...  
Keyword(s):  
Acute Rejection ◽  
Skin Biopsy ◽  
Peripheral Blood ◽  
Biomarker Discovery ◽  
Serum Proteins ◽  
Transplant Rejection ◽  
Treatment Options ◽  
Face Transplantation ◽  
Reasonable Solution ◽  
Promising Solution

AbstractFace transplantation is a promising solution for patients with devastating facial injuries who lack other satisfactory treatment options. At the same time, this type of transplantation is accompanied with high risks of acute transplant rejection. The limitations of traditional skin biopsy and the need to frequently monitor the condition of face transplant call for less invasive biomarkers to better diagnose and treat acute rejection. Discovery of peripheral serum proteins accurately reflecting the transplant status would represent a reasonable solution to meet this demand. However, to date, there is no clinical data available to address the feasibility of this approach. In this study, we used the next generation aptamer-based SOMAscan proteomics platform to profile 1305 proteins of peripheral blood serum in twenty-four samples taken from 6 patients during no-rejection, nonsevere rejection, and severe rejection episodes. Also, we provide a detailed description of biosample processing and all steps to generate and analyze the SOMAscan dataset with hope it will assist in performing biomarker discovery in other transplantation centers using this platform.

scholarly journals Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers

2017 ◽  
Vol 67 (2) ◽  
pp. 201-221 ◽  
Author(s):  
Marijana Kovačić ◽  
Dragana Marković ◽  
Irina Maslovarić ◽  
Sonja Obrenović ◽  
Jelica Grujić-Milanović ◽  
...  
Keyword(s):  
Acute Phase ◽  
Peripheral Blood ◽  
Pasteurella Multocida ◽  
Serum Proteins ◽  
Acute Phase Proteins ◽  
Clinical Signs ◽  
Hdl Cholesterol ◽  
Matrix Metalloproteases ◽  
Γ Globulins ◽  
Biochemical Analyses

Abstract Calf bronchopneumonia is complex multifactorial disease and for its accurate diagnosis and therapy, besides clinical examination, microbiologic, hematologic and biochemical analyses could be necessary. In general, additional analyses are not implemented, mainly because the disease biomarkers are not defined. To establish which analysis might be useful for determining the severity of the disease, we analyzed 23 three-month old calves with mild clinical signs of bronchopneumonia and 15 age-matched healthy calves. Pasteurella multocida was isolated from deep nasal swabs of diseased calves. Peripheral blood erythrocyte and leukocyte count of bronchopneumonic and healthy calves showed no difference. Serum proteins, lipoproteins and lipids were analyzed with spectrophotometry, agarose gel electrophoresis, non-reducing SDS-PAGE, gel zymography, and thin-layer chromatography. The bronchopneumonic calves had an increased level of circulating immune complexes and α globulins, which contain some of the positive acute phase proteins. In diseased calves the increased concentration of total γ globulins (IgG), due to an increased concentration of anionic γ globulins (predominately IgG1), was detected. The increased concentration of anionic γ globulins followed by increased concentration of transferrin (negative acute phase protein) and HDL cholesterol, decreased concentration of LDL-cholesterol, unchanged activity of matrix metalloproteases and leukocyte counts might reflect the obvious absence of generalized inflammation. A positive correlation was found between the acquired results and the appearance of mild clinical signs. Therefore, we believe that the parameters analyzed in the peripheral blood could be applied as reliable disease markers to distinguish between severe (inflammatory) and mild forms of calf bronchopneumonia and to predict a better outcome for these calves.

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